scholarly journals First outbreak of bovine haemorrhagic septicaemia caused by Pasteurella multocida type B in Spain – Short communication

2020 ◽  
Vol 68 (1) ◽  
pp. 8-11 ◽  
Author(s):  
Inmaculada Cuevas ◽  
Alfonso Carbonero ◽  
David Cano ◽  
Isabel L. Pacheco ◽  
Juan C. Marín ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Economic Losses ◽  
Type B ◽  
Beef Calves ◽  
Haemorrhagic Septicaemia ◽  
Source Of Infection ◽  
Initial Stage ◽  
Pure Cultures ◽  
Ventral Area ◽  
Autogenous Vaccine

AbstractThis paper describes the first documented outbreak of haemorrhagic septicaemia (HS) caused by Pasteurella multocida type B in cattle in Spain. This acute, highly fatal septicaemia causes major economic losses in cattle and buffaloes in many areas of Asia and Africa. In other species and in European countries it is an infrequently reported disease. Acute septicaemic pasteurellosis occurred in a free-range farm of 150 cattle and 70 beef calves in Southern Spain. Twenty-one calves and one cow were affected, of which three calves and the adult cow died. Postmortem examination revealed characteristic oedema in the ventral area of the neck and the brisket region, and widespread haemorrhages in all organs. Pure cultures of P. multocida were obtained from all tissues and organs studied. The aetiological agent was further confirmed by molecular and biochemical analysis as P. multocida capsular type B, biovar 3. Although the source of infection could not be determined, wildlife may play an important role. The use of tulathromycin in the initial stage of the disease might be related to the low morbidity and mortality of this outbreak. After using an autogenous vaccine no more cases of HS were observed.

scholarly journals Mass spectrometric analysis of lipid A obtained from the lipopolysaccharide of Pasteurella multocida

RSC Advances ◽  
2020 ◽  
Vol 10 (51) ◽  
pp. 30917-30933
Author(s):  
Abdul Tawab ◽  
Noor Akbar ◽  
Mujtaba Hasssan ◽  
Fazale Habib ◽  
Aamir Ali ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pasteurella Multocida ◽  
Lipid A ◽  
Water Buffalo ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Type B ◽  
Haemorrhagic Septicaemia

LC/MS-based variant profiling of lipid A component of endotoxic lipopolysaccharides of Pasteurella multocida type B:2, a causative agent of haemorrhagic septicaemia in water buffalo and cattle.

scholarly journals First detection of Pasteurella multocida type B:2 in Hungary associated with systemic pasteurellosis in backyard pigs

2015 ◽  
Vol 63 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Barbara Ujvári ◽  
Levente Szeredi ◽  
László Pertl ◽  
Gergely Tóth ◽  
Károly Erdélyi ◽  
...  
Keyword(s):  
Small Area ◽  
Pasteurella Multocida ◽  
Sequence Type ◽  
Type B ◽  
Chain Reaction ◽  
Haemorrhagic Septicaemia ◽  
Gene Typing ◽  
Polymerase Chain ◽  
Serotype B ◽  
Backyard Pigs

This is the first report of Pasteurella multocida type B in Hungarian pigs. This disease was observed in backyard-raised pigs in three households within a small area. Neither the source of the infection nor the epidemiological connection between any of the premises could be determined. The most consistent lesion was dark red discolouration of the skin of the ventral neck and brisket, with accompanying oedema and haemorrhages. The morbidity was low and lethality relatively high, with three dead (50%) and two euthanised (33%) out of six affected animals. A total of three isolates of P. multocida (P55, P56 and P57) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. All were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST61) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host. M13 polymerase chain reaction and virulence-associated gene typing also show that type B strains form a highly homogeneous, distinct phylogenic group within P. multocida.

scholarly journals Re-emergence of bovine haemorrhagic septicaemia in Hungary

2017 ◽  
Vol 65 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Tibor Magyar ◽  
Barbara Ujvári ◽  
Levente Szeredi ◽  
Norbert Virsinger ◽  
Ervin Albert ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Animal Health ◽  
Small Farm ◽  
Beef Calves ◽  
Haemorrhagic Septicaemia ◽  
Lymph Node Enlargement ◽  
Laboratory Examinations ◽  
Serotype B ◽  
Loss Of Appetite ◽  
World Organisation

This paper reports an outbreak of haemorrhagic septicaemia caused by Pasteurella multocida B:2 in beef calves, a disease that has not been described in the Hungarian literature since 1943, and has not been reported to the World Organisation For Animal Health (OIE) since 1970. Acute haemorrhagic septicaemia was confirmed in beef calves on one small farm, and was suspected on two further nearby holdings with concomitant unexplained losses. The source of the infection could not be determined. Apart from a short duration of depression and loss of appetite, the affected calves developed characteristic distal limb oedema. Gross findings in two calves submitted for laboratory examinations included subcutaneous oedema and haemorrhages on serous membranes, and in one case severe pharyngeal lymph node enlargement was observed. Histological examinations revealed lesions characteristic of septicaemia. Moderate to large amounts of Pasteurella antigens were detected in all organs tested by immunohistochemistry. Two isolates of P. multocida (Pm240, Pm241) were cultured from these cases and examined in detail. These were identified as P. multocida ssp. multocida biovar 3. Both were toxA negative and belonged to serotype B:2. Multilocus sequence typing was used to assign these to a new sequence type (ST64) that is closely related to other haemorrhagic septicaemia causing strains of P. multocida regardless of the host.

scholarly journals PREPARATION AND COMPARATIVE EVALUATION OF HAEMORRHAGIC SEPTICAEMIA VACCINES USING EOLANE-150 AND EOLANE-170 AS OIL ADJUVANTS FOR CATTLE AND BUFFALO

2020 ◽  
Vol 36 (1) ◽  
pp. 78-84
Author(s):  
W. Shahzad ◽  
B. Zameer ◽  
S. H. Sanghi ◽  
S. Hussain ◽  
N. Mustafa
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Dry Weight ◽  
Economic Losses ◽  
Bacterial Antigen ◽  
Serum Samples ◽  
Prophylactic Measure ◽  
Post Vaccination ◽  
Haemorrhagic Septicaemia ◽  
Serotype B

Haemorrhagic septicaemia (HS) caused by Pasteurella multocida serotype B:2 is an economically important disease of cattle and buffalo, causes heavy economic losses due to sudden death of animals in developing countries like Pakistan. In this country, animals are vaccinated by alum (adjuvant) precipitated vaccine twice a year. Immunity induced through this prophylactic measure lasts for 3-4 months only. Two new HS oil based vaccines were prepared by using two new oil adjuvants such as Eolane-150 and Eolane-170. The ratio of bacterial antigen and oil adjuvants was 1:1 while bacterial dry weight was adjusted to 2 mg/ml. The addition of enrichments and aeration resulted in dense bacterial growth of Pasteurella multocida. Both new vaccines passed sterility, safety and potency tests as per OIE, 2017. Active and passive Mouse Protection Tests were performed to evaluate its potency. Indirect Haem-Agglutination (IHA) test was conducted on serum samples of two rabbits, groups each of which was vaccinated with HS oil based vaccines adjuvanted with Eolane-150 and Eolane-170. IHA indicated that immune response was higher (GMT=32) initially on 45th day to 75th day post vaccination and then declined (GMT=16) in the rabbits vaccinated with HS vaccine adjuvanted with Eolane-150, while protective immune response remained constant (GMT=16) up to ninety days post vaccination in the rabbits vaccinated with HS vaccine adjuvanted with Eolane-170. Vaccines were easy to inject with no side effects, including swelling at the injection site and longer protection as well. That would hopefully motivate the livestock owners and farmers to use this new product to protect their animals against this fatal HS disease.

Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

2019 ◽  
Vol 15 (02) ◽  
pp. 22-25
Author(s):  
Sunaina Thakur ◽  
Subhash Verma ◽  
Prasenjit Dhar ◽  
Mandeep Sharma
Keyword(s):  
Pasteurella Multocida ◽  
Direct Detection ◽  
Bacterial Species ◽  
Economic Losses ◽  
Isolation And Identification ◽  
Sheep And Goats ◽  
Histophilus Somni ◽  
Nasal Swabs ◽  
Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

scholarly journals Mapping, cluster detection and evaluation of risk factors of ovine toxoplasmosis in Southern Italy

2016 ◽  
Vol 11 (2) ◽  
Author(s):  
Roberto Condoleo ◽  
Vincenzo Musella ◽  
Maria Paola Maurelli ◽  
Antonio Bosco ◽  
Giuseppe Cringoli ◽  
...  
Keyword(s):  
Risk Factors ◽  
Geographical Information Systems ◽  
Southern Italy ◽  
Geographical Information ◽  
Economic Losses ◽  
Common Source ◽  
High Exposure ◽  
Campania Region ◽  
Source Of Infection ◽  
Serological Data

Toxoplasmosis, an important cause of reproductive failure in sheep, is responsible for significant economic losses to the ovine industry worldwide. Moreover, ovine meat contaminated by the parasite <em>Toxoplasma gondii</em> is considered as a common source of infection for humans. The aim of this study was to develop point and risk profiling maps of <em>T. gondii</em> seroprevalence in sheep bred in Campania Region (Southern Italy) and analyse risk factors associated at the flock-level. We used serological data from a previous survey of 117 sheep flocks, while environmental and farm management information were obtained from an analysis based on geographical information systems and a questionnaire purveyance, respectively. An univariate Poisson regression model revealed that the type of farm production (milk and meat vs only meat) was the only independent variable associated with <em>T. gondii</em> positivity (P&lt;0.02); the higher within-flock seroprevalence in milking herds suggests that milking practices might influence the spread of the infection on the farm. Neither environmental nor other management variables were significant. Since a majority of flocks were seasonally or permanently on pasture, the animals have a high exposure to infectious <em>T. gondii</em> oocysts, so the high within-flock seroprevalence might derive from this management factor. However, further studies are needed to better assess the actual epidemiological situation of toxoplasmosis in sheep and to clarify the factors that influence its presence and distribution.

scholarly journals Molecular Identification and Epidemiological Tracing of Pasteurella multocida Meningitis in a Baby

2000 ◽  
Vol 38 (3) ◽  
pp. 1235-1237 ◽  
Author(s):  
Patrick Boerlin ◽  
Hans H. Siegrist ◽  
André P. Burnens ◽  
Peter Kuhnert ◽  
Purita Mendez ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Close Contact ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Source Of Infection ◽  
Subspecies Level ◽  
History Of ◽  
Rrna Gene Sequencing ◽  
The One

We report a case of Pasteurella multocida meningitis in a 1-month-old baby exposed to close contact with two dogs and a cat but without any known history of injury by these animals. 16S rRNA gene sequencing of the isolate from the baby allowed identification at the subspecies level and pointed to the cat as a possible source of infection. Molecular typing of Pasteurella isolates from the animals, from the baby, and from unrelated animals clearly confirmed that the cat harbored the same P. multocidasubsp. septica strain on its tonsils as the one isolated from the cerebrospinal fluid of the baby. This case stresses the necessity of informing susceptible hosts at risk of contracting zoonotic agents about some basic hygiene rules when keeping pets. In addition, this study illustrates the usefulness of molecular methods for identification and epidemiological tracing ofPasteurella isolates.

scholarly journals Molecular identification by multiplex polymerase chain reaction of Pasteurella multocida in cattle and buffaloes in Baghdad

2014 ◽  
Vol 38 (1) ◽  
pp. 99-106
Author(s):  
Ihab G. M. AL-Shemmari
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Multiplex Polymerase Chain Reaction ◽  
Pasteurella Multocida ◽  
Type B ◽  
Chain Reaction ◽  
Blood Samples ◽  
Nasal Swabs ◽  
Polymerase Chain ◽  
B 31

The aim of this study was to identify pasteurella multocida and their types by PCR in cattle’s and buffaloesi bagdad from March to August 2012 on 204 animals , including 102 cattle and 102 buffaloes at slaughter houses from Baghdad .Blood samples and nasal swaps were collected , before slaughtering and lung tissues of slaughtered animal , and from 54 clinically suspected cases of pasteurellosis , including 27 bovines ,and 27 buffaloes the samples taken included blood and nasal swabs . Pasteurellamultocida were isolated from 94 animals include 49 cattle 45 buffaloes. The typing of the isolates by multiplex PCR for genotyping Pasteuerllamultocida revealed 93 isolates of type B , 31 from cattle and 62 from buffaloes ,and 81 isolates of type A , 55 from cattle and 26 from buffaloes .

Sign in / Sign up

Export Citation Format

Share Document