scholarly journals PRRS eradication from swine farms in five regions of Hungary

Author(s):  
István Szabó ◽  
Lajos Bognár ◽  
Tamás Molnár ◽  
Imre Nemes ◽  
Ádám Bálint

AbstractPorcine reproductive and respiratory syndrome (PRRS) causes significant losses to the swine industry worldwide, which leads to launching eradication programmes. The PRRS eradication programme in Hungary is based on the territorial principle, and it is obligatory for each swine farm irrespective of the number of animals kept there. Hungary has an exceptionally large herd size in large-scale pig farms. Large fattening farms operate as all-in/all-out or continuous flow systems. The large-scale breeding herds are predominantly farrow-to-finish types. In large-scale breeding farms, PRRS eradication was carried out by the depopulation-repopulation method in 33 farms, of which 23 received state compensation, 18 farm units either finished production or changed to producing fatteners only. Two farms used the test and removal method for eradication. One farm was classified as ‘vaccinated free’. At this farm the breeding animals are vaccinated continuously but there is no vaccination of the progeny at any age, and the PRRS-free status of the farm is strictly controlled and monitored. By 31 December 2019, all pigs in five euroregions of Hungary had become free from PRRS virus, while the PRRS eradication process is still ongoing in the remaining two regions.

2019 ◽  
Vol 67 (4) ◽  
pp. 543-552 ◽  
Author(s):  
Imre Nemes ◽  
Tamás Molnár ◽  
Tamás Abonyi ◽  
Zsolt Terjék ◽  
Ádám Bálint ◽  
...  

In the EU Member States with a traditionally significant pig industry, the prevalence of PRRS infections is high. Therefore, the Pig Strategy of the Government of Hungary prioritises eradication of PRRSV in Hungary. For the first time among the EU Member States, a National PRRS Eradication Programme was introduced in order to reach a more efficient, economical and competitive international market position. Although its significance has decreased in recent decades, 20% of the Hungarian pig population is still kept on small-scale (backyard) farms (< 100 animals). The prevalence of PRRSV in backyard farms was 3.9% at the beginning of the programme. The present paper details the measures applied during the different phases of the programme in backyard farms. During all the phases, serological testing of the breeding animals of the registered small-scale herds was performed, including the highest number of individual animals. Seropositive individuals were tested by PCR and were removed from the backyard farm within the framework of official measures. By sequencing the identified PRRS strains, the possible epidemic relationships between small-scale and large-scale farms were continuously monitored. As a result of the programme, PRRS-free status of the small-scale herds was achieved by the end of 2015, and this status was maintained in 2016-2018.


2014 ◽  
Vol 2 (1) ◽  
pp. 30-37
Author(s):  
Ashish Chandra ◽  
Dr. A. K. Dubey ◽  
Dr. Sachin Kumar Srivastava

This study covered 150 cooperative member milk producers and 150 non-member milk producers which were post- stratified into Landless, Marginal, small, medium and large herd size categories. Breakeven point is a point where no profit no loss status achieved where MR = MC. In this study breakeven point analysis was done to estimate the minimum quantity milk to be produced to cover the total cost on all categories (members and nonmembers) of households of milch animals (Cow and buffalo). And also in this study the researchers have find out the Total cost of milk production per liter for member and non member categories. This study is helpful to find out the total cost of milk production in all categories as well as members and nonmembers of dairy cooperative society are able to find out the breakeven point of the whole business.


2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Hongfang Ma ◽  
Rui Li ◽  
Longguang Jiang ◽  
Songlin Qiao ◽  
Xin-xin Chen ◽  
...  

AbstractPorcine reproductive and respiratory syndrome (PRRS) is a serious disease burdening global swine industry. Infection by its etiological agent, PRRS virus (PRRSV), shows a highly restricted tropism of host cells and has been demonstrated to be mediated by an essential scavenger receptor (SR) CD163. CD163 fifth SR cysteine-rich domain (SRCR5) is further proven to play a crucial role during viral infection. Despite intense research, the involvement of CD163 SRCR5 in PRRSV infection remains to be elucidated. In the current study, we prepared recombinant monkey CD163 (moCD163) SRCR5 and human CD163-like homolog (hCD163L1) SRCR8, and determined their crystal structures. After comparison with the previously reported crystal structure of porcine CD163 (pCD163) SRCR5, these structures showed almost identical structural folds but significantly different surface electrostatic potentials. Based on these differences, we carried out mutational research to identify that the charged residue at position 534 in association with the one at position 561 were important for PRRSV-2 infection in vitro. Altogether the current work sheds some light on CD163-mediated PRRSV-2 infection and deepens our understanding of the viral pathogenesis, which will provide clues for prevention and control of PRRS.


2012 ◽  
Vol 13 (1) ◽  
pp. 21-37 ◽  
Author(s):  
Gourapura J. Renukaradhya ◽  
Varun Dwivedi ◽  
Cordelia Manickam ◽  
Basavaraj Binjawadagi ◽  
David Benfield

AbstractPorcine reproductive and respiratory syndrome (PRRS) is an economically important infectious disease of swine. Constant emergence of variant strains of PRRS virus (PPRSV) and virus-mediated immune evasion followed by viral persistence result in increased incidence and recurrence of PRRS in swine herds. Current live and killed PRRSV vaccines administered by a parenteral route are ineffective in inducing complete protection. Thus, new approaches in design and delivery of PRRSV vaccines are needed to reduce the disease burden of the swine industry. Induction of an effective mucosal immunity to several respiratory pathogens by direct delivery of a vaccine to mucosal sites has proven to be effective in a mouse model. However, there are challenges in eliciting mucosal immunity to PRRS due to our limited understanding of safe and potent mucosal adjuvants, which could potentiate the mucosal immune response to PRRSV. The purpose of this review is to discuss methods for induction of protective mucosal immune responses in the respiratory tract of pigs. The manuscript also discusses how PRRSV modulates innate, adaptive and immunoregulatory responses at both mucosal and systemic sites of infected and/or vaccinated pigs. This information may help in the design of innovative mucosal vaccines to elicit superior cross-protective immunity against divergent field strains of PRRSV.


2017 ◽  
Vol 58 (2) ◽  
pp. 157 ◽  
Author(s):  
V. G. PAPATSIROS (Β.Γ. ΠΑΠΑΤΣΙΡΟΣ) ◽  
C. ALEXOPOULOS (Κ. ΑΛΕΞΟΠΟΥΛΟΣ) ◽  
S. C. KYRIAKIS (Σ.Κ. ΚΥΡΙΑΚΗΣ)

The clinical manifestations of PRRS virus infection vary from subclinical to severe reproductive failure and/or respiratory disease. Severity of PRRS virus associated disease may result from interactions among factors involving differences in virulence among PRRS virus isolates, probable recombination between the different isolates that are responsible for the clinical manifestation of PRRS in the same farm, differences in concurrent infections (other viruses and bacteria) and hygiene monitoring programme. It is known that viruses and bacteria interacted and the most common model of this interaction is that pathogens bacteria complicate the lesions that are due to viruses. PRRS virus induces predisposition to Streptococcus suis in nursery age pigs and increases susceptibility to Salmonella choleraesuis, Bordetella bronchiseptica and Mycoplasma hyopneumoniae. Furthermore, PRRS virus is involved in aetiology and predispose to appearance of Porcine Respiratory Disease Complex (PRDC) Postweaning Multisystemic Wasting Syndrome (PMWS) and Porcine Dermatitis and Nephropathy Syndrome (PDNS). The respiratory form of PRRS co-infection with Aujeszky' disease causes severe losses in swine herds due to respiratory problems. In cases of PRRS co-infections with other agents, the cost of pork meat production is raised from the treatments and other special management strategies. Up to date, in Greece, PRRS is appeared with the enzootic form (independendy of the season) with elevations and declines. In Greek swine industry, PRRS virus is involved as primary pathogen agent in cautions of mortality of growing/finishing pigs. During the last years, the presence of the new syndromes of PRDC, PMWS and PDNS causes additional economical losses and increases the cost of Greek pork meat production. The unpublished data of editors indicate that during last year, PRRS caused severe losses in Greek swine industry. Cases of PRRS were noticed in farms which purchased gilts or piglets without keeping out preventive facilities of biosecurity as quarantine, serology examinations etc. The syndrome was appeared with the respiratory form in growing/finishing pigs associated with severe losses, due to the entrance of «new» different isolates of PRRS virus from the purchase of gilts or piglets. It is probably that these «new» different isolates were more virulent than the preexisted isolates or/and predominated with them. In cases, where severe respiratory signs in growing/finishing pigs and no or moderate reproductive form of PRRS were noticed, it is probable that the vaccinations of sows against PRRS did not protect the growing/finishing pigs or the «new» different isolates of PRRS virus had more respiratory than reproductive tropism or coexistenced in all earlier circumstances. Today, PRRS is a dangerous risk factor for the Greek swine industry and for this reason it is important to apply all preventive facilities as: vaccinations, reduction of the introduction gilts and maintenance of a grandparent nucleus in the farm for producing gilts, quarantine and serological monitoring for all introduced animals (gilts and piglets), as well as the purchase of animals from PRRS-negative farms. The purchase of piglets has to be avoided because it induces negative effects on the clinical manifestation and control of PRRS.


2018 ◽  
Vol 51 ◽  
pp. 41-60 ◽  
Author(s):  
M. V. Gladiy ◽  
Yu. P. Polupan ◽  
I. V. Bazyshina ◽  
A. E. Pochukalyn ◽  
T. P. Koval ◽  
...  

Ukrainian population of red dairy cattle is genetically active, its gene pool is significantly improved in each of the next generations, mainly due to artificial selection. Currently breeding structure of Ukrainian Red Dairy breed has the prospect of future expansion due to creating Podolian zonal type. Features of its formation are to use as the original breed, which being improved, Red Polish cattle. This breed, as Red Steppe cattle, was listed as a breed to preserve the gene pool under pure breeding. However, gene pool preservation of these breeds is impossible through lack of sufficient number of purebred bulls or their semen at breeding enterprises in Ukraine. The aim of our research was to study genesis and development prospects of red dairy cattle in Ukraine. Materials and methods of research. The materials of the research were the data of identifying breed composition of cattle in Ukraine by January 1, 2015, State Breeding List for 2005, 2010 and 2015, the electronic information database of leading breeding farms for 2007 and 2011 and database of bulls admitted to using during 2005, 2010 and 2015 of Institute of Animal Breeding and Genetics named after M.V. Zubets of NAAS. Results. Currently the group of cows of red breeds is about 365 thousand head, including 97.9 thousand head concentrated in agricultural enterprises. The largest share of this group takes the Red Steppe cattle (84.5%), the smallest one –Ayrshire and Angler breeds (0.7-0.8%). In Ukraine, the total number of Ukrainian Red Dairy cattle is about 40 thousand head, including 21.4 thousand head of cattle concentrated in agricultural enterprises. The results of these annual reports have shown that the average milk yield of the group of cows of red breeds was 3328 kg of milk in 2001. Then milk yield increased with each subsequent studied period and reached the highest figure of 4681 kg in 2014. So, increasing milk yield of cows was by 1353 kg of milk or 29% during fourteen years (2001-2014). The number of breeding animals of Red Steppe, Red Polish, Ayrshire, and Ukrainian Red Dairy breeds significantly decreased in the controlled part of population during the past fourteen years. However, level of milk production and quality increased slightly, except for Red Polish breed. Thus, 4688 cows of Ukrainian Red Dairy breed had milk yield in 5981 kg and milk fat content in 3.88% according to the appraisal of 2014, whereas, in 2013, 4902 cows had milk yield in 5837 kg with milk fat content in 3.86%. Up to 90% of the realized genetic progress in large-scale breeding is provided by using proven bulls, resulting most current need is to restore the national system of selection and testing of sires. The results show that during the past ten years, the number of proven bulls, admitted to using within the red breeds decreased almost seven times with a simultaneous increase 2.2 times in breeding value. Among 410 proven bulls, admitted to using in 2005 (bulls which being tested by progeny), unreasonably disproportionate share was for Holstein sires – 245 (59.8%), whereas, the share of bulls of the red breeds – 13.7%. In 2010, 123 bulls (65.4%) were of Holstein breed and only 9 ones (4.8%) – of Ukrainian Red Dairy among 188 bulls, admitted to using. In 2014, a similar situation was observed; there were 93 bulls, admitted to using, including 48 ones (51.6%) of Holstein and only 3 ones (3.2%) of Ukrainian Red Dairy breed. Given the current state of livestock breeding base on all the red breeds (Red Steppe, Ukrainian Red Dairy, Angler, Red Polish) we developed possible options of parameters of large-scale breeding. During the planning period the number of the controlled cows of the red breeds (mainly Ukrainian Red Dairy cattle) should be significantly increased. Calculations show that in the first stage of the program available number of cows in breeding farms, even if 30% of cows allocated to mating with laid-off bulls, is less than a third of the minimum at pressure of selection among laid-off bulls 1: 4 by productivity of 50 daughters. At the end of the program it is planned to increase the pressure of selection among laid-off bulls to 1:5 under increase of the controlled livestock number to 43700 cows (nearly 4.4 times against currently available one) Conclusion. Our results showed that, the number of breeding farms, in which were bred red cattle, significantly declined and the livestock number also decreased whereas milk production increased during 2001-2015. The high level of genetic potential of Ukrainian Red Dairy cows indicates the presence of highest-yielding cows with yield more than 10 000 kg of milk. At the same time, such animals at breeding farms for breeding Red Polish, Steppe, Ayrshire and Angler cattle weren’t found. During the past ten years, the number of proven bulls of red breeds, admitted to using, decreased almost seven times with a simultaneous increase of breeding value in 2.2 times. Predominance of Holstein bulls remains indisputable on reliability of assessment of breeding value. It causes the biggest request and the widest offer on the market of semen from bulls of the breed. The actual destruction of domestic system of sires breeding caused significantly lower rate of repeatability of breeding values in bulls of the red breeds. This is a concern and threatens possible loss or significant restriction of the gene pool of red breeds.


2019 ◽  
Vol 67 (4) ◽  
pp. 529-542 ◽  
Author(s):  
István Szabó ◽  
Tamás Molnár ◽  
Imre Nemes ◽  
Tamás Abonyi ◽  
Zsolt Terjék ◽  
...  

Eradication of porcine reproductive and respiratory syndrome virus (PRRSV) from the pig population of Hungary started in 2014 on the basis of the territorial principle. In order to reach this goal it was crucial to render each fattening unit free of this disease, since fattening units play a significant role in spreading the virus all over the country. In 2015, 188 out of 307 large-scale fattening farms (61.2%) kept PRRS-positive animals. The main source of infection of these farms was the import of PRRS-infected fattening pigs. The following methods were used during the eradication from 2017: (1) Only pigs coming from PRRS-free farms were allowed to be used for fattening in Hungary; (2) Quarantine of all herds for 60 days; (3) PCR test for PRRS 48 hours after the arrival of the prefattening animals; (4) Serological test for PRRS at the end of the quarantine period. If any diagnostic test gave even one positive result and the result was confirmed by another test, the stock had to be sold for slaughter within 15 days or placed outside Hungary, so that the infected stock would not compromise the PRRS status of that area. PRRSV eradication on large-scale fattening units applying all-in/all-out operation was relatively simple, using the depopulation-repopulation method. On permanently operating farms, the infected herd was sold from time to time, without having to be repopulated until the last delivery. After cleaning, disinfection and restocking, the repopulation was done with PRRS-free animals. As the eradication progressed over the years, a ban on the import of infected fattening pigs was imposed. As a consequence of these measures, by the end of 2018, Hungarian large-scale fattening farms became free of PRRS. Maintaining the national-level PRRS-free status of large-scale pig fattening units contributes to eliminating a significant cost factor from the Hungarian pork production industry, and opens the way for a significant reduction in antibiotic consumption as well.


2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yuzo Koketsu ◽  
Ryosuke Iida

Abstract Our objectives in this review are 1) to define the four components of sow lifetime performance, 2) to organize the four components and other key measures in a lifetime performance tree, and 3) to compile information about sow and herd-level predictors for sow lifetime performance that can help producers or veterinarians improve their decision making. First, we defined the four components of sow lifetime performance: lifetime efficiency, sow longevity, fertility and prolificacy. We propose that lifetime efficiency should be measured as annualized piglets weaned or annualized piglets born alive which is an integrated measure for sow lifetime performance, whereas longevity should be measured as sow life days and herd-life days which are the number of days from birth to removal and the number of days from date of first-mating to removal, respectively. We also propose that fertility should be measured as lifetime non-productive days, whereas prolificacy should be measured as lifetime pigs born alive. Second, we propose two lifetime performance trees for annualized piglets weaned and annualized piglets born alive, respectively, and show inter-relationships between the four components of the lifetime performance in these trees. Third, we describe sow and herd-level predictors for high lifetime performance of sows. An example of a sow-level predictor is that gilts with lower age at first-mating are associated with higher lifetime performance in all four components. Other examples are that no re-service in parity 0 and shorter weaning-to-first-mating interval in parity 1 are associated with higher fertility, whereas more piglets born in parity 1 is associated with higher prolificacy. It appears that fertility and prolificacy are independent each other. Furthermore, sows with high prolificacy and high fertility are more likely to have high longevity and high efficiency. Also, an increased number of stillborn piglets indicates that sows have farrowing difficulty or a herd health problem. Regarding herd-level predictors, large herd size is associated with higher efficiency. Also, herd-level predictors can interact with sow level predictors for sow lifetime performance. For example, sow longevity decreases more in large herds than small-to-mid herds, whereas gilt age at first-mating increases. So, it appears that herd size alters the impact of delayed gilt age at first-mating on sow longevity. Increased knowledge of these four components of sow lifetime performance and their predictors should help producers and veterinarians maximize a sow’s potential and optimize her lifetime productivity in breeding herds.


2019 ◽  
Vol 18 (06) ◽  
pp. 8-13
Author(s):  
Phat X. Dinh

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important diseases to swine industry worldwide. Due to the heterogeneity of field isolates, accurate detection of the PRRS virus is a diagnostic challenge. Recently, co-infection with NA-PRRSV, EU-PRRSV and HP-PRRSV isolates continuously increases in many countries, resulting in a significant impact on PRRSV diagnostics and disease control on farms. To facilitate rapid diagnosis and reliable discrimination of NA-PRRSV, EU-PRRSV and HP-PRRSV, a multiplex RT-PCR assay was established with three pairs of primers targeting highly conservative regions of nsp2 gene with predicted multiplex RT-PCR products of 364 bp, 161 bp and 259 bp, respectively. The primer pairs were optimized to be highly specific for PRRSV genotypes and were able to detect the target gene at the limit of 102 copies/μL for each gene. Clinical samples were used to evaluate this multiplex RT-PCR in parallel with a commercial real-time RT-PCR kit. Results showed over 95.2% (20/21 samples) agreement between the mRT-PCR and the real-time RT-PCR kit. Hence, it indicated that this multiplex RT-PCR could be useful for rapid and deferential diagnosis of NA-PRRSV, EU-PRRSV and HP-PRRSV in swine farms.


2018 ◽  
Vol 40 (2) ◽  
pp. 177-183
Author(s):  
Dao Thi Sen ◽  
Chu Hoang Ha ◽  
Nguyen Tuong Van

PRRS (Porcine reproductive and respiratory syndrome) is the most significant infectious disease currently devastating the swine industry. In order to diversify antigen sources for vaccine production to against PRRSV, co-expressing LTB and PRRSV GP5- an important structure protein GP5 of PRRSV were expressed in tobacco. Genes encoded for GP5 of PRRSV isolated from pigs infected PRRSV in Vietnam in 2010 and was constructed in plant expression vector under the control of seed specific phaseolin promoter and constitutive promoter 35S. The genes were then transferred into the C9-1 tobacco using Agrobacterium. The stable insertion of LTB-GP5genes in genomeoftransgenic tobacco was confirmed by PCR and RT-PCR. Western hybridization indicated that fused protein LTB-GP5 was successful expressed in tobacco seeds. Analysis of transgenic tobaco seeds revealed that under regulation of promoter beta-phaseolin, the level expression of GP5 was significant improved as quantified by ELISA (2.2 % of total soluble protein). This preliminary result is a foundation for expression of GP5 in seeds of other plants in the future.


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