Microbiological quality aspects of ready-to-eat foods with focus on antibiotic resistance and biofilm formation abilities of foodborne bacteria

2021 ◽  
Vol 50 (3) ◽  
pp. 433-441
Author(s):  
D. Aksoy
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Microbiological Quality ◽  
Staphylococcal Enterotoxin ◽  
Penicillin G ◽  
Aerobic Bacteria ◽  
Staphylococcal Enterotoxin A ◽  
Quality Aspects

AbstractIn this study, microbiological quality of 93 ready-to-eat food products was determined by enumeration of total aerobic bacteria and members of Enterobacteriaceae. Presence of Staphylococcus aureus and Listeria monocytogenes in the products were also investigated. Aerobic colony counts were between 1.9 × 102–3.4 × 108 CFU g−1 for 84.9% and Enterobacteriaceae counts were between 2 × 102 and 6.7 × 106 CFU g−1 for 43% of the samples. S. aureus was detected in 7.5% of the samples, but L. monocytogenes was not detected in any sample. 72.9% of the Enterobacteriaceae isolates showed resistance to at least one antibiotic tested, and 5 among the S. aureus isolates were found to be resistant to penicillin G and 2 against methicillin. Four S. aureus isolates produced only Staphylococcal enterotoxin A and 1 isolate produced both Staphylococcal enterotoxin A and B. Biofilm experiments revealed biofilm formation on polystyrene for 95.8%, 87.5%, and 91.6% of Enterobacteriaceae isolates at 4 °C, 22 °C, and 37 °C, respectively, whereas all S. aureus isolates formed biofilm at all temperatures.

Investigation of the antibiotic resistance and biofilm formation of Staphylococcus aureus strains isolated from gangrenous mastitis of ewes

2012 ◽  
Vol 60 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Osman Tel ◽  
Özkan Aslantaş ◽  
Oktay Keskin ◽  
Ebru Yilmaz ◽  
Cemil Demir
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Factor ◽  
Resistance Genes ◽  
Biofilm Formation ◽  
Antibiotic Resistance Genes ◽  
Penicillin G ◽  
Beta Lactamase ◽  
Potential Virulence Factor ◽  
Amoxicillin Clavulanic Acid

In this study,Staphylococcus aureusstrains (n = 110) isolated from seven ewe flocks in Sanliurfa, Turkey were screened for antibiotic resistance and biofilmforming ability as well as for genes associated with antibiotic resistance and biofilm-forming ability. All isolates were found to be susceptible to oxacillin, gentamicin, clindamycin, cefoxitin, tetracycline, vancomycin, amoxicillin-clavulanic acid, ciprofloxacin and sulphamethoxazole-trimethoprim. The percent proportions of strains resistant to penicillin G, ampicillin and erythromycin were 27.2% (n = 30), 25.4% (n = 28) and 6.3% (n = 7), respectively. Regarding the antibiotic resistance genes, 32 (29%) isolates carried theblaZ and 8 (7.2%) theermC gene. Other resistance genes were not detected in the isolates. All isolates showed biofilm-forming ability on Congo red agar (CRA), while 108 (98.18%) and 101 (91.81%) of them were identified as biofilm producers by the use of standard tube (ST) and microplate (MP) methods, respectively. All isolates carried theicaA andicaD genes but none of them harboured thebapgene. The results demonstrated thatS. aureusisolates from gangrenous mastitis were mainly resistant to penicillins (which are susceptible to the staphylococcal beta-lactamase enzyme), and less frequently to erythromycin. Furthermore, all of theS. aureusisolates produced biofilm which was considered a potential virulence factor in the pathogenesis of staphylococcal mastitis.

Microbiological Quality of Terrestrial Gastropods Prepared for Human Consumption

2004 ◽  
Vol 67 (8) ◽  
pp. 1779-1781 ◽  
Author(s):  
S. SERRANO ◽  
L. M. MEDINA ◽  
M. JURADO ◽  
M. JODRAL
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Aerobic Bacteria ◽  
Human Consumption ◽  
Terrestrial Gastropods ◽  
Hygienic Quality ◽  
And Staphylococcus Aureus ◽  
Spanish Legislation

Hygienic quality was investigated in 60 samples of ready-to-eat snails (Helix lactea, Helix nemoralis, and Helix hortensis) obtained from 30 in-season street stalls and 30 catering establishments (bars) in Córdoba. In accordance with Spanish legislation regarding hygiene in the preparation, distribution, and sale of prepared foods, counts were obtained for total mesophilic aerobic bacteria, coliforms, total Enterobacteriaceae, Escherichia coli, Salmonella, and Staphylococcus aureus. The results obtained showed that 33.3, 20.0, and 13.3% of the samples from stalls and 73.3, 53.3, and 6.7% of the samples from bars did not comply with the regulations with respect to the control of total mesophilic aerobic bacteria, Enterobacteriaceae, and Staphylococcus aureus, respectively. Coliforms were found in 53.3% of the samples from the bars (mean, 2.1 CFU/g) and in 33.3% of the stalls (mean, 1.1 CFU/g). Salmonella was not detected.

scholarly journals Microbiological Quality of Food and Ready Meals Sold in Fast Food Restaurants on the Campus of NANGUI ABROGOUA University in Abidjan (Ivory coast)

2021 ◽  
Vol 10 (11) ◽  
pp. 226-234
Author(s):  
YAO Kouadio DAN Chépo Ghislaine ◽  
NANGA Yessé Zinzendorf KOMADE Thierry ◽  
LOUKOU Yao Guillaume KOUAME Lucien Patrice
Keyword(s):  
Staphylococcus Aureus ◽  
Fast Food ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Aerobic Bacteria ◽  
Colony Forming Unit ◽  
Consumer Safety ◽  
Fast Food Restaurants ◽  
Quality Of Food

The objective of this study was to analyze the microbilogical quality of foods sold in fast food restaurants on the campus of NAGUI ABROGOUA University (UNA). The detection and enumeration of total mesophilic aerobic bacteria, total and fecal coliforms, Salmonella, Staphylococcus aureus, sulfite-reducing anaerobes (ASR) and Clostridium perfringens were carried out according to the standard methods in force. In 3 months, 36 samples of ready meals from 7 sites were collected and analyzed. The results obtained revealed that the germ loads (colony forming unit : CFU) vary according to the nature of the germ, its origin and the nature of the food sampled. The charges in GAM vary from 9.4 108 ± to 1.5 108 CFU/g to 6.3 101 ± 4.5 101 CFU/g. Overall, attiéké-garba and its condiments contain most of the germs sought with the exception of Salmonella which are absent in all the dishes analyzed. Thus, in terms of microbiological quality, 60 %, 33.3 %, 25 % and 13.3 % of the samples taken respectively from the school office aera, IREN - market and fixed restaurant sites are of non-microbiological quality satisfying the standards. This non-compliance is more attributable to attiéké-garba and its condiments contaminated with coliform strains, in particular Escherichia coli and strains of Staphylococcus aureus. It is therefore necessary to improve the hygienic quality of meals served in fast food restaurants on the campus of NANGUI ABROGOUA University (UNA), in particular attiéké-garba condiments and curdled milk to ensure better consumer safety.

Plasmid Profiles and Pulsed-Field Gel Electrophoresis for Type A Enterotoxigenic Staphylococcus aureus Isolated from Foods

1995 ◽  
Vol 58 (2) ◽  
pp. 147-153 ◽  
Author(s):  
HAU-YANG TSEN ◽  
GEE-KAITE YU ◽  
IN-TING LIN
Keyword(s):  
Staphylococcus Aureus ◽  
Gel Electrophoresis ◽  
Food Samples ◽  
Pulsed Field Gel Electrophoresis ◽  
Staphylococcal Enterotoxin ◽  
Penicillin G ◽  
Staphylococcal Enterotoxin A ◽  
Chromosomal Dna ◽  
Pulsed Field ◽  
Plasmid Profiles

Reports regarding the use of plasmid profile and pulsed-field gel electrophoresis (PFGE) in subtyping of specific enterotoxigenic Staphylococcus aureus isolated from food samples are limited. We isolated 176 strains of S. aureus from food samples and showed that 64 of these strains are enterotoxigenic as determined by staphylococcal enterotoxin A, B, C, D detection kit by reversed passive latex agglutination (SET-RPLA) method. Of these 64 enterotoxigenic strains, 45 by SET-RPLA or 44 by polymerase chain reaction (PCR) were identified to be staphylococcal enterotoxin A (SEA) producing strains. All these 64 enterotoxigenic strains could be grouped into three patterns of antibiogram when their antibiotic susceptibilities to penicillin G, oxacillin, vancomycin and methicillin were investigated. Strains resistant to penicillin G, but susceptible to the other three antibiotics were of the major pattern since 50 of the 64 enterotoxigenic strains tested showed such pattern of antibiotic susceptibility. Plasmid deoxyribonucleic acid (DNA) analysis for the 45 SEA strains showed that four types of plasmid profiles could be obtained. When chromosomal DNA of these SEA strains were digested with Smal and subjected to the PFGE analysis, a total of eight genome types were observed. In addition, 28 of these 45 SEA strains tested could be grouped into one specific type of these eight genome types. Results shown in this report may be useful for the epidemiological study and for the tracing of contamination source when a food poisoning outbreak occurs.

Determination of antibiotic resistance and enterotoxigenic potential of Staphylococcus aureus strains isolated from foods sold by street vendors in Gaborone, Botswana

2017 ◽  
Vol 6 (04) ◽  
pp. 5334 ◽  
Author(s):  
Daniel Loeto ◽  
Kabo Wale ◽  
Tidimalo Coetzee ◽  
Krishna B. Khare* ◽  
Thabang Carol Sigwele ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Food Poisoning ◽  
Food Samples ◽  
Latex Agglutination ◽  
Staphylococcal Enterotoxin ◽  
Penicillin G ◽  
Personal Hygiene ◽  
Street Vendors ◽  
Antibiotic Resistance Profile

Staphylococcus aureus is one of the causes of foodborne diseases worldwide. Staphylococcal food poisoning ensues after ingestion of contaminated food and results in symptoms of gastroenteritis such as vomiting, abdominal cramps and diarrhea. The present paper aims to isolate Staphylococcus aureus from foods sold by street vendors in Gaborone, Botswana, and to determine its enterotoxigenic potential and antibiotic resistance profile. One hundred eight food samples comprising starch, meat, salads and vegetables portions were collected from these vendors and tested for the presence of S. aureus. Identification of Staphylococcus aureus to the species level was performed using the Vitek 2 automated identification and susceptibility testing system (BioMerieux, Marcy-I’Etoile, France). Enterotoxins were detected by the Reversed Passive Latex Agglutination method (SET-RPLA). Results showed that 49 (45%) of the samples tested positive for Staphylococcus aureus. The organism was isolated at higher frequencies in vegetables and starchy foods (34.7%) than in meats (30.6%). These differences in isolation rates however, were not statistically significant (p> 0.05). Staphylococcus aureus isolates were found to be resistant to penicillin G (52.4%), tetracycline (38.1%), methicillin (26.2%) and vancomycin (11.9%). Four Staphylococcal enterotoxin types A-D, were detected among the isolates. Staphylococcal enterotoxin D was the most prevalent (52.9%), while enterotoxin C was produced by the least number of isolates (5.9%). Of note, five isolates simultaneously expressed two or more enterotoxin types in varying combinations. The present study underscores a potential risk of staphylococcal food poisoning and transmission of methicillin resistant S. aueus strains for consumers of street vended food products in Gaborone, Botswana especially in the absence of a quality assurance regulatory framework. As a mitigating factor, sensitization of street food vendors on the importance of food and personal hygiene is strongly recommended.

Microbiological Examination of Vegetable Seed Sprouts in Korea

2009 ◽  
Vol 72 (4) ◽  
pp. 856-859 ◽  
Author(s):  
HOIKYUNG KIM ◽  
YOUNGJUN LEE ◽  
LARRY R. BEUCHAT ◽  
BONG-JUNE YOON ◽  
JEE-HOON RYU
Keyword(s):  
Microbiological Quality ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Enterobacter Sakazakii ◽  
Department Stores ◽  
E Coli ◽  
Traditional Markets ◽  
Radish Sprouts ◽  
Online Stores

Sprouted vegetable seeds used as food have been implicated as sources of outbreaks of Salmonella and Escherichia coli O157:H7 infections. We profiled the microbiological quality of sprouts and seeds sold at retail shops in Seoul, Korea. Ninety samples of radish sprouts and mixed sprouts purchased at department stores, supermarkets, and traditional markets and 96 samples of radish, alfalfa, and turnip seeds purchased from online stores were analyzed to determine the number of total aerobic bacteria (TAB) and molds or yeasts (MY) and the incidence of Salmonella, E. coli O157:H7, and Enterobacter sakazakii. Significantly higher numbers of TAB (7.52 log CFU/g) and MY (7.36 log CFU/g) were present on mixed sprouts than on radish sprouts (6.97 and 6.50 CFU/g, respectively). Populations of TAB and MY on the sprouts were not significantly affected by location of purchase. Radish seeds contained TAB and MY populations of 4.08 and 2.42 log CFU/g, respectively, whereas populations of TAB were only 2.54 to 2.84 log CFU/g and populations of MY were 0.82 to 1.69 log CFU/g on alfalfa and turnip seeds, respectively. Salmonella and E. coli O157:H7 were not detected on any of the sprout and seed samples tested. E. sakazakii was not found on seeds, but 13.3% of the mixed sprout samples contained this potentially pathogenic bacterium.

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from <3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

scholarly journals Tokat Bez Sucuğunun Mikrobiyolojik Kalitesinin İncelenmesi

2020 ◽  
Vol 8 (12) ◽  
pp. 2683-2694
Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Microbiological Parameters

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform

scholarly journals Prevalence and Characterization of Antibiotic-Resistant Staphylococcus aureus Recovered from Pasteurized Cheese Commercialized in Panama City Markets

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Fermín Mejía ◽  
Nohelia Castro-del Campo ◽  
Arleny García ◽  
Katerine Rodríguez ◽  
Humberto Cornejo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Food Poisoning ◽  
Microbiological Quality ◽  
Gastrointestinal Diseases ◽  
Antibiotic Resistant ◽  
Panama City ◽  
Antibiotic Resistance Profile ◽  
White Cheese ◽  
High Degree

Foodborne bacteria, with a high degree of antibiotic resistance, play an important role in the morbidity and mortality of gastrointestinal diseases worldwide. Among 250 disease-causing bacteria, Staphylococcus aureus is one of the major causes of food poisoning, and its resistance to multiple antimicrobials remains of crucial concern. Cheese is often contaminated when proper sanitary procedures are not followed during its production and marketing. This work aimed to evaluate the microbiological quality of pasteurized white cheese commercialized in Panama City. Cheese from five different brands sold in local supermarkets were selected to determine the presence of S. aureus as well as its antibiotic resistance profile. The results showed significant contamination of S. aureus with a geometric median sample of 104–107 CFU/g. Four out of five (4/5) cheese brands analyzed presented risk of food poisoning by exceeding the allowed range of consumption with a geometric median sample of 1,8 × 106–1,4 × 107 CFU/g. Fourteen different resistance phenotypes were found. Fifty-five percent (55%) of the analyzed strains were resistant to erythromycin. The data confirm a relatively high prevalence and high levels of S. aureus, most likely originated during handling in Panama City retail markets. Further studies are needed to reduce bacterial contamination and to decrease the risk of food poisoning in the consumption of pasteurized cheese.

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