Transformation of Nitrogen Fertilizers in Greenhouse Experiments
The primary (1 st year) and the after-effects (2 nd , 3 rd year) of N fertilizers (KNO 3 , NH 4 Cl) on the soil-plant-atmosphere system were studied in a three-year greenhouse pot experiment with and without maize plants. The two- and three-year balances of the fertilizer N uptake and gaseous N losses were also analyzed. The cumulative values of the gaseous losses showed a similar trend in all years, significant differences were not obtained. On the basis of the three-year balance, the gaseous loss in the planted and unplanted pots was 18-22% and about 37-39%, respectively. Consequently, there was a 50% decrease in denitrificated gaseous losses of fertilizer N due to plant N uptake. The cumulative gaseous loss, calculated by the difference method, was significantly higher in cases of KNO 3 applications than in NH 4 Cl treatments, as an assumed consequence of the intensive denitrification. It was found that the gaseous loss was not influenced by soil moisture. In contrast to the gaseous losses, the values of plant N uptake and soil mineral N content showed significant differences in the years studied, as a result of the quick transformation of mineral N to organic N, the non-complete homogenization of the total soil amount, the seasonal climatic differences in the greenhouse during the years studied, and consequently the different microbiological activity. The plant N uptake was found to depend significantly on the fertilizer N form. Results obtained by the difference method and the 15 N-tracer technique were very similar. In the case of KNO 3 treatment and higher soil moisture (WHC = 80%) plant N uptake was more intensive, ranging between 48-57% (calculated by the difference method), and 35-51% (calculated by the 15 N- tracer method) in the first year (1993). It can be concluded that 60-100% of the fertilizer N was used from the soil by plant uptake and gaseous losses, which depends mainly on the treatments and the soil moisture during the first year. These values changed between 7-17% in the 1 st year after-effect and between 1-5% in the 2 nd year after-effect.