RP-HPLC AND HPTLC METHODS FOR THE ESTIMATION OF LUPEOL IN. HEMIDESMUS INDICUS LINN. ROOT EXTRACTS AND POLYHERBAL FORMULATION USING STANDARD MA

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (07) ◽  
pp. 40-46
Author(s):  
Jayaprakasam Rajendran ◽  
Anita Elizabeth Chacko ◽  
Tresa Thomas ◽  
Neethu Johnson ◽  
Kochupapy Ravi Thengungal
Keyword(s):  
Correlation Coefficient ◽  
Chemical Constituents ◽  
Hemidesmus Indicus ◽  
Polyherbal Formulation ◽  
Ich Guidelines ◽  
Soxhlet Apparatus ◽  
The Family ◽  
Herbal Plants ◽  
Hptlc Method

The roots of Hemidesmus indicus, belonging to the family Ascelpidaceae, are used as blood purifier, antileucorrhoeic, galactogenic, antidiarrhoeal, antirheumatic, antisyphilitic, febrifuge and possesses chemical constituents like triterpenoids (lupeol), flavonoids, glycosides and tannins. Roots were dried and extracted with petroleum ether, chloroform and methanol by using Soxhlet apparatus. Two simple and sensitive chromatographic methods, namely, HPTLC and HPLC, were developed for determination of lupeol from the extract of H. indicus and its polyherbal formulation and validated according to ICH guidelines. The HPTLC method linear regression data for the standard lupeol a concentration range of 400-900ng/spot and correlation coefficient (r) was 0.9904. The HPLC calibration curves of standard lupeol showed good linearity range from 20 to 100μg/ml and correlation coefficient (r) was 0.9929. The HPTLC and HPLC methods are simple, precise, accurate and specific. Hence, these methods can be used for the standardization of lupeol in herbal plants and polyherbal formulations.

Comparative Studies of Chemical Constituents of Hibiscus rosa-sinensis Using Different Extraction Techniques

2020 ◽  
Vol 981 ◽  
pp. 296-301
Author(s):  
Nur Salsabila Ahmad Roslan ◽  
Seema Zareen ◽  
Normaiza Zamri
Keyword(s):  
Essential Oil ◽  
Bioactive Compounds ◽  
Capillary Column ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hibiscus Rosa Sinensis ◽  
Soxhlet Apparatus ◽  
The Family

Hibiscus rosa-sinensis which belongs to the family Malvaceae, is a national flower of Malaysia. This glabrous tree can usually grow around 1 to 3 meters tall. The objective of present study was to categorize the essential oil constituent of flowers and leaves extract using different extraction method namely Soxhlet and hydrodistillation. The essential oil obtained from Soxhlet (10.75%) and hydrodistillation (11.40%) were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) for determination of bioactive compounds. The GC-MS analysis was carried out by Agilent 7980A series GC instrument and DB-1MS capillary column with the dimensions of 30 m × 0.25 mm. A total of 21 and 20 compounds were identified for Soxhlet and hyrodistillation technique, respectively. Major compound found in essential oil for Soxhlet apparatus method was (Z,Z,Z)-9-12,15-Octadecatrienoic acid, methyl ester (23.59%) while for hydrodistillation was 1-Methylene-2b-hydroxymethyl-3,3-dimethyl-4b-(3-methylbut-2-enyl)-cyclohexane (16.57%). The presence of numerous bioactive compounds provides insights to the potential of the Hibiscus rosa-sinensis in pharmaceutical industry.

DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF SELEGILINE HYDROCHLORIDE LOADED SOLID LIPID NANOPARTICLES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 57-60
Author(s):  
J. B Prajapati ◽  
H Rao ◽  
H Shah ◽  
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Laboratory Scale ◽  
Solid Lipid ◽  
Ich Guidelines ◽  
Development And Validation

The present paper discusses about a simple, precise and validated method for the determination of selegiline loaded solid lipid nanoparticles. The study was carried as per the parameters laid down in ICH guidelines. Maximum wavelength of selegiline in 8:2 methanol: chloroform mixture was selected at 258nm. The method was found to be linear in the range of 200μg/mL to 1000μg/mL with correlation coefficient R2 of 0.994. Method was successfully validating as per ICH guidelines. Moreover, this method was simple, sensitive and easy to apply and can be performed at laboratory scale. Hence, the proposed method can be used for analysis of determination of selegiline loaded solid lipid nanoparticles.

scholarly journals IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

2017 ◽  
Vol 9 (9) ◽  
pp. 126
Author(s):  
Pratheema Philomindoss
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
Chromatography Method ◽  
Ich Guidelines ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

VALIDATED HPTLC METHOD FOR SIMULTANEOUS DETERMINATION OF OLMESARTAN MEDOXIMIL AND METOPROLOL SUCCINATE IN TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (10) ◽  
pp. 13-17
Author(s):  
V. V Kunjir ◽  
◽  
S. B. Jadhav ◽  
A. J Purkar ◽  
P. D. Chaudhari
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Metoprolol Succinate ◽  
Ich Guidelines ◽  
Tablet Dosage ◽  
Hptlc Method

A high performance thin layer chromatographic method has been developed for the simultaneous determination of olmesartan medoximil and metoprolol succinate from tablet dosage form. The mobile phase consisting of water-methanol-ammonium sulphate (4.5:4.5:1.5 v/v/v) and wavelength of detection 233 nm was used. The developed method was validated as per ICH guidelines.

Determination of Compounds inside Inoculated Aquilaria malaccensis Leaves by Soxhlet Extraction

2015 ◽  
Vol 1113 ◽  
pp. 267-272 ◽  
Author(s):  
Ku Halim Ku Hamid ◽  
Nur Suhada Mustafar ◽  
Pazilah Mohd Yusof ◽  
Siti Sofia Mohammad Shawal ◽  
Mohibah Musa ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Specific Detail ◽  
The Earth ◽  
Aquilaria Malaccensis ◽  
The Family ◽  
The World ◽  
Different Types

Aquilaria is a special tree that has been awarded to the earth. Aquilaria spp. which belongs to the family Thymelaeceae is one of the most precious trees is the world. Agarwood or gaharu is the name of the resin produced by Aquilaria spp. Historically, agarwood has widely been used as medicine. It was also being used for perfumes, incense and religious purposes in Muslim, Buddhist, Hindu, Christian, and Jewish societies. In order to discover the new uses of the agarwood, knowledge of the chemical constituents inside the plant need to be known. Aquilaria spp tree can be divided into two parts which is bark and leaves. Recently, several studies have been conducted to examine the chemical composition found in Aquilaria spp tree. However, there is still no specific detail documentation on the chemical compounds present in Aquilaria leaves. In this study, Soxhlet extraction method has been used to withdraw the compounds from the leaves. From the results obtained, it shows that extraction of compounds by using methanol was more effective. This was due to the higher polarity inside the methanol. Other than solvents, the samples preparation also gave an effect towards the final results. There were two different types of samples that have been used for this study which are fresh and dried A. malaccensis leaves. The obtaining results showed that more compounds have been extracted from the dried sample. It can be conclude that moisture content in the sample also plays a role in identification of compounds inside the A. malccensis leaves

scholarly journals A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation

2019 ◽  
Vol 7 (1) ◽  
pp. 7-13
Author(s):  
Vijaykumar K. Parmar ◽  
Deepika Mohanta ◽  
Harsh Shah
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Simultaneous Estimation ◽  
Berberine Chloride ◽  
Herbal Formulation ◽  
Ich Guidelines ◽  
System Suitability ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68 ± 0.02, respectively. The response in terms of peak area was found to be linear over the concentration range of 100-500 ng/spot for berberine chloride and 200-1000 ng/spot for guggulsterone Z with regression coefficient value greater than 0.995 for both the phytoconstituents. The method was validated by determining its accuracy, precision, robustness, specificity and system suitability. The method was found to be accurate, precise and robust to carry out the simultaneous estimation of berberine chloride and guggulsterone Z. The developed method was successfully applied for the simultaneous estimation of berberine chloride and guggulsterone Z in herbal formulation.

HPTLC Method for Simultaneous Determination of Piperine, Embeline, and Carvone in the Ayurvedic Formulation Catpusphadhya Churna

2014 ◽  
Vol 97 (3) ◽  
pp. 773-777 ◽  
Author(s):  
Kajal L Jain ◽  
Rakesh K Patel ◽  
Hardik P Patel
Keyword(s):  
Raw Materials ◽  
Important Method ◽  
Important Group ◽  
Therapeutic Value ◽  
Soxhlet Apparatus ◽  
Materials Used ◽  
Indian Medicine ◽  
Hptlc Method ◽  
Detection And Quantification

Abstract Churnas are an important group of formulations used by traditional physicians to treat various types of diseases. The principle of using a churna is based on the fact that the therapeutic value of most substances greatly increases when they are reduced to a very fine state of subdivision. Catpusphadhya churna, as per the Ayurvedic system of Indian medicine, is used for acute rheumatoid arthritis. In the present study, an attempt was made to develop an HPTLC method for the quantitative determination of piperine, embeline, and carvone in a laboratory-prepared formulation. Raw materials used in formulations were obtained from two different suppliers and were subjected to methanol extractions by using a Soxhlet apparatus. Piperine, embeline, and carvone were quantified in the extracts by using HPTLC. The detection and quantification were performed at 254 nm. The formulation contained 2.35% (w/w) of piperine, 4.86% (w/w) of embeline, and 1.48% (v/w) of carvone. Linearity studies indicated that piperine, embeline, and carvone were in the linear ranges, while the recovery studies revealed a recovery of 99.32% (w/w) of piperine, 101.82% (w/w) of embeline, and 100.09% (v/w) of carvone, thus proving the accuracy of the analysis. The developed HPTLC method resolved and quantified piperine, embeline, and carvone effectively, so it could be an important method for the QC of polyherbal formulations.

scholarly journals Quantification of Gymnemagenin and β-Sitosterol in Marketed Herbal Formulation by Validated Normal Phase HPTLC Method

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sachin E. Potawale ◽  
Satish Y. Gabhe ◽  
Kakasaheb R. Mahadik
Keyword(s):  
High Performance ◽  
Research Study ◽  
Retention Factor ◽  
Solvent System ◽  
Polyherbal Formulation ◽  
Herbal Formulation ◽  
Acid Reagent ◽  
Development And Validation ◽  
Hptlc Method

This research study describes development and validation of new, rapid, accurate, robust, and precise, high performance thin layer chromatographic (HPTLC) method for concurrent quantitative determination of gymnemagenin and β-sitosterol in herbal formulation with densitometric detection. Chromatographic separation was achieved on Merck aluminum HPTLC plates precoated with silica gel 60 F254. The optimized solvent system consisted of toluene : ethyl acetate : methanol (6.5 : 2.5 : 1.4, v/v/v). Developed plates were derivatized with 5% sulphuric acid reagent followed by heating at 110°C for 4 min in a preheated oven and scanned at 423 nm in reflectance-absorbance mode. The retention factor for gymnemagenin and β-sitosterol was found to be 0.27±0.02 and 0.78±0.02, respectively. The proposed densitometric method was validated according to ICH Q2 (R1) guidelines. Results were found to be linear over a range of 100–1200 ng band−1 and 200–1200 ng band−1 for gymnemagenin and β-sitosterol, respectively. The percent content of gymnemagenin and β-sitosterol in the marketed polyherbal formulation was found to be 0.0405% and 0.1377%, respectively. The proposed HPTLC method can be used for quantification of gymnemagenin and β-sitosterol in marketed polyherbal formulation used in the study in quality-control laboratories.

scholarly journals Pharmacognostical and phytochemical studies on leaves of Oxalis corniculata Linn

2016 ◽  
Vol 5 (6) ◽  
pp. 225-229
Author(s):  
Shalini Karunanithi ◽  
◽  
Vijaya Bharathi Rajkishore ◽  
Vandhana. Ganesh Pol ◽  
Sitharathul Mumthana. M Mumthana. M ◽  
...  
Keyword(s):  
Research Work ◽  
Fluorescence Analysis ◽  
Water Soluble ◽  
Physiochemical Parameters ◽  
Soxhlet Apparatus ◽  
The Family ◽  
Annual Herb ◽  
Cardiac Disorders ◽  
Phytochemical Studies

Oxalis corniculata Linn is an annual herb commonly known as Indian horrel belonging to the family Oxalidaceae. It is found to uses as, anthelminitic, astringent, diuretic, emmenagogue, febrifuge, cardiac disorders, diarrheoa, dysmenorrhoea, hepatic disorders, eye disease, scurvy and toxicity. This research work deals with the Pharmacognostical and Phytochemical studies on leaves of Oxalis corniculata Linn microscopic photographs of transverse section (T.S) of Oxalis corniculata should distinct presentation of epidermal trichomes, calcium oxalate crystals and stomata. Standardization of the plant were done with the help of extractive values [ alcohol soluble extractive (16%w/w), water soluble extractive (24%w/w)], total ash (15%w/w), acid insoluble ash (1.7%w/w), water soluble ash (1.8%w/w), sulphated ash (0.9%w/w). Pytochemical analysis showed the presence of carbohydrates, glycosides, flavanoids, phenols, tannins, volatile oils, aminoacids and proteins. The dried coarsely powdered Oxalis corniculata plant was extracted with ethanol 600C in soxhlet apparatus for 24 hours. Another extract is prepared in cold maceration method for 7 days using water. The both extract was collected and preserved in a vaccum desicator. Fluorescence analysis was carried out for the plant powder and extract. This study various Pharmacognostical and physiochemical parameters for the identification and standardization of the plant material and Phytochemical screening for determination of active constituents.

scholarly journals Feronia elephantum Correa: A Potential Traditional Drug

2021 ◽  
Vol 12 (3) ◽  
pp. 482-484
Author(s):  
Mrinal Sharma ◽  
Sanjay Nagdev ◽  
Mayur Bhurat ◽  
Amitkumar Dhankani ◽  
Mayur Jain ◽  
...  
Keyword(s):  
South India ◽  
Chemical Constituents ◽  
Wide Range ◽  
The Family ◽  
Traditional Drug ◽  
Herbal Plants ◽  
Ancient Times ◽  
Diseased Condition ◽  
Feronia Elephantum ◽  
Different Parts

There are number of herbal plants used as medicines from the ancient time. Chemical constituents from those plants isolated for wide range of diseased condition utilizes in from the ancient times in Ayurveda, Unani, Siddha etc. systems. Feronia elephantum Correa from the family Rutaceae also known as Kaith, Wood apple is one of them which have many chemical constituents with important medicinal purposes and diseases like Diabetes mellitus, ulcer, as diuretic, liver tonic etc. Leaves of the plant are astringent, carminative and prescribed for vomiting, dysentery, hiccough, indigestion and slight bowel affections of children. The fruits are used as dessert, source of beverages and juices. The fruit pulp is used externally as a remedy for certain insect bite. The bark is aromatic and cooling, useful in vitiated conditions of pitta. It is indigenous in South India, Java, Myanmar, Malaysia and Ceylon and cultivated in many parts of India. This plant is already used traditionally in Sri Lanka for many purposes like vomiting, dysentery etc. Chemical constituents isolated from the plant different parts already investigated for many pharmacological and pharmaceutical activities like Anti-ulcer, Antidiabetic, Antimicrobial, Antibacterial, Antioxidant, cytotoxic, Antitumor, Antihistaminic, Hepatoprotective, Antifungal, Antifertility and Anti-inflammatory. This article is an attempt to compile all the details of plant Feronia elephantum Correa.

Sign in / Sign up

Export Citation Format

Share Document