Investigation of the effect of supramaximal eccentric contractions on muscle damage and recovery between the dominant and non-dominant arm

Purpose: This research aimed to examine the effects of supramaximal eccentric contractions on the damage of muscle and recovery between the dominant and non-dominant arm. The study was participated by ten male volunteer students who do not have any musculoskeletal and metabolic compliant. This research designed as a cross-over type research. Material: Venous blood samples were drawn before the bout, immediately after the bout one min., moreover, at the 6th, 24th, 48th, and 72nd hours after training. Data were analyzed using the SPSS 21.0 statistical software package. After exercise, the non-parametric Wilcoxon Signed Ranks Test was used to decide the dominant and non-dominant arm influence. Statistical significance for all analyses was accepted at p< 0,05. Results: There was a significant statistical difference in the right, and left arm Creatine Kinase (CK) values at the time when exercise start, 48th and 72nd hours (p<0,05). There was no statistically significant difference in right and left arm Lactate Dehydrogenase values after exercise (p>0,05). Conclusions: There was an increase in muscle damage at 24th and 48th hours after supramaximal eccentric exercise, and it was turned back the start level at 72nd hours. While the arm which is non-dominant recovery is faster than the dominant arm.

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Cardio-respiratory and biochemical responses of military training event at Jungle environment

Asian Journal of Medical Sciences ◽

10.3126/ajms.v12i4.33923 ◽

2021 ◽

Vol 12 (4) ◽

pp. 15-23

Author(s):

Angad Yadav ◽

Tirthankar Chatterjee ◽

Debojyoti Bhattacharyya ◽

Somnath Singh ◽

Madhusudan Pal

Keyword(s):

Core Body Temperature ◽

Statistical Significance ◽

Military Training ◽

Venous Blood ◽

Post Exercise ◽

Biochemical Responses ◽

Significant Difference ◽

Training Event ◽

Training Activities ◽

Exercise Induced

Background: In military environment, soldiers regularly practice or undergo different types of extreme training activities. However, globally the literatures available on the physiological and biochemical demand of different extreme military training activities are very scanty and less reported. Aims and Objective: The present study was undertaken to quantify the cardio-respiratory and biochemical responses of military training event in jungle environment. Materials and Methods: Mathew’s Mad Mile (MMM) activity is a type of specialized run of 1.5 mile in jungle environment. This training activity was conducted on rugged jungle terrain comprised of undulated uphill, downhill, muddy surface. Twenty-five SHAPE-1 healthy soldiers were volunteered into training event. Cardiorespiratory data was recorded continuously throughout the event and venous blood sample was drawn before and immediately after completion of the event. Statistical significance was considered at p<0.05. Results: There was no significant difference observed in heart rate and breathing rate while core body temperature was significantly (p = 0.02) higher in slow finisher as compared to fast finisher. In fast finisher, post exercise level of BDNF, BNP, SDH, cortisol and UCP1 increased significantly (p<0.05), whereas, BHB (p<0.01) decreased significantly in comparison to pre-exercise. In slow finishers, post exercise level of cortisol, KYNA and UCP1 increased significantly (p<0.05), whereas, BDNF, BNP and SDH decreased significantly (p<0.05), in comparison to pre-exercise. Conclusion: The outcome of this study indicated that the slow finishers were more susceptible to risk of injury due to higher exercise induced thermogenesis and mental stress in comparison to fast finisher.

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High preanalytical non-compliance and samples rejection rate in clinical biochemistry laboratory are decreased by nurse staff training in phlebotomy and sample handling

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2020.13.3.0400 ◽

2020 ◽

Vol 13 (3) ◽

pp. 113-118

Author(s):

Modibo Coulibaly ◽

Abdelaye Keita ◽

Moussa Diawara ◽

Valentin Sagara ◽

Brehima Traoré ◽

...

Keyword(s):

Staff Training ◽

Clinical Biochemistry ◽

Statistical Significance ◽

Venous Blood ◽

Diagnostic Errors ◽

Hospital Staff ◽

Clinical Samples ◽

Sample Handling ◽

Significant Difference ◽

Clinical Biochemistry Laboratory

Background: Preanalytical phase of biomedical analysis remains an important source of diagnostic errors that deserves special attention. This study aims to evaluate the training in phlebotomy and sample handling impact on the preanalytical non-compliances. Material and Methods: we performed a prospective study before and after staff training in phlebotomy and sample handling by systematically recording all clinical samples non-compliances. First, we assessed and describe the non-compliance baseline rate from January to December 2017 in the clinical biochemistry laboratory of Hôpital Sominé DOLO de Mopti. After two sessions of one week staff training in January 2018, we performed the same study from January to December 2018. We compared the proportions of non-compliances between the two assessments. Data were collected on the case report forms, captured in Excel and analyzed by R software for (Mac) OS X version 4.0.3. Pearson Ch2 or Fisher exact tests were used for the comparison of proportions. The statistical significance was set at p < 5%. Results: a total of 27,810 venous blood samples were received during the study period; 48% was for biochemistry, 41% for immuno-serology, 9% for blood cell count and 2% for coagulation tests. There were 3,826 instances of preanalytical non-compliances (13.76%) identified that led to sample rejection. Out of the 11 types of non-compliances investigated, 5 (45.4%) accounted for nearly 91% of the problems: insufficient sample volume (28.9%), hemolyzed samples (20.5%), inappropriate collection time (17.8%), sample clot (12.9%), and inappropriate sample collection tube (10.8%). We observed a significant difference in rates of non-compliance between inpatients and outpatients samples (44.4% vs 7.3%; p < 0.001). The proportion of non-compliance have significatively decreased after the two training sessions of hospital staff in phlebotomy and sample handling 3,826/27,810 (13.8%) vs 3,009/32,476 (9.3%); p < 0.001. Conclusion: we report a significantly higher rate of non-compliance in inpatients. Hospital staff training in phlebotomy and sample handling reduce the proportion of preanalytical non-compliance and thereby improve patient management and safety.

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Sexual Dimorphism in Laryngeal Volumetric Measurements Using Magnetic Resonance Imaging

Ear Nose & Throat Journal ◽

10.1177/0145561319840568 ◽

2019 ◽

Vol 99 (2) ◽

pp. 132-136 ◽

Cited By ~ 1

Author(s):

Abdul-Latif Hamdan ◽

Elie Khalifee ◽

Georges Ziade ◽

Sahar Semaan

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Statistical Significance ◽

Resonance Imaging ◽

Men And Women ◽

Mean Width ◽

Significant Difference ◽

The Mean ◽

The Right ◽

Volumetric Measurements

The objective of this study is to investigate the dimensional and volumetric measurements in the thyroarytenoid (TA) muscle in men and women using magnetic resonance imaging (MRI). The hypothesis is that there is a gender-related difference in these measurements. A retrospective chart review of 76 patients who underwent MRI of the neck at the American University of Beirut Medical Center was conducted. The dimension and volume of the right and left TA muscle were measured on axial and coronal planes short tau inversion recovery images. Male and female groups were compared with respect to demographic data and MRI findings using parametric and nonparametric tests. The mean length of the thyro-arytenoid muscle in males was larger than that in females on the right (males 2.44 [0.29] cm vs females 1.70 [0.22] cm) and on the left (males 2.50 [0.28] cm vs females 1.72 [0.24] cm) reaching statistical significance ( P < .001). The mean width of the thyro-arytenoid muscle in males was larger than that in females on the right (males 0.68 [0.13] cm vs females 0.59 [0.11] cm) and on the left (males 0.68 [0.12] cm vs females 0.57 [0.12] cm) reaching statistical significance ( P < .001). The mean height of the thyro-arytenoid muscle in males was larger than that in females on the right (males 1.05 [0.21] cm vs females 0.95 [0.12] cm) and on the left (males 1.05 [0.21] cm vs females 0.95 [0.12] cm) reaching statistical significance ( P < .01 on the right and P < .05 on the left). The volume of the thyroarytenoid muscle in males was larger than that in females on the right (males 0.86 [0.25] mL vs females 0.48 [0.15] mL) and on the left (males 0.89 [0.27] mL vs females 0.48 [0.17] mL) reaching statistical significance ( P < .001). The results of this investigation clearly indicate a significant difference in these measurements between men and women.

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Does Cervicogenic Headache Result in the Presence of Neural Tension, and Does this Affect the Position and Mobility of Atlas?

Medical & Clinical Research ◽

10.33140/mcr.06.01.02 ◽

2021 ◽

Vol 6 (1) ◽

Keyword(s):

Statistical Significance ◽

Axial Rotation ◽

Cervicogenic Headache ◽

Upper Cervical Spine ◽

Slump Test ◽

Significant Difference ◽

Cervicogenic Headaches ◽

Upper Cervical ◽

The Right ◽

Straight Leg Raise

Background: Cervicogenic headaches (CGH) can be the result of dysfunction of the upper cervical spine. Due to the soft tissue connection between muscle, cervical fascia, and dura, this region might contribute to the development of CGH. Objective: Evaluate if subjects with CGH have neural tension signs. The secondary objective was to investigate if a correlation between the position and mobility of atlas and cervicogenic headaches exists. Methods: 60 Subjects were recruited. Self-reported outcome measures, passive neck flexion rotation test, upper limb tension test (ULTT), slump test, and straight leg raise test (SLR) were assessed. Results: There was a significant difference in atlanto-axial rotation to the right with p=0.025. There was no statistical significance in left rotation. There was no significant relationship between CGH, ULTT, slump and SLR with P>0.05. Atlas position was significantly related to CGH with P<0.001, and position of the atlas was significantly related to atlanto-axial motion with p<0.001. Discussion: There is a direct relationship between the position and mobility of atlas and CGH. Fascial connections between structures could result in dural tension and should be considered when managing patients with CGH. The use of the ULTT, Slump test, and SLR test does not appear to be beneficial in identifying those with CGH.

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Effect of Sparring Training Model on Liver Enzymes and Muscle Damage in Boxers

Journal of Education and Training Studies ◽

10.11114/jets.v7i3s.4152 ◽

2019 ◽

Vol 7 (3S) ◽

pp. 114

Author(s):

Ömer Kaynar

Keyword(s):

Muscle Damage ◽

Liver Enzymes ◽

Venous Blood ◽

Training Model ◽

Acute Effect ◽

Main Theme ◽

Significance Level ◽

Significant Difference ◽

Before And After ◽

Liver Region

The purpose of this study is to examine the acute effect of sparring training model on liver enzymes and muscle damage in boxers.14 voluntary athletes engaged in box (23.71±1.44 years of age; 167±5.89 cm; 62.05±8.89 kg) participated in the study. Sparring training model composed of warm-up (25 minutes), main part (30 minutes) and cool-down (15 minutes) exercises was applied to the boxers. The main theme of the study is composed of the free sub-stroke sparring training model in the liver region. Sparring training applied in the liver region and consisting of 3-minute time periods was repeated 3 times with a 1-minute interval as in the punch bag training. In venous blood samples taken from the study group before and after sparring training, AST, ALT and GGT enzyme activities and CK and LDH levels were analyzed with the spectrophotometric method in Beckman Coulter AU 5800 auto analyzer. Data were analyzed in SPSS 22.0 package program. The significance level was accepted as p<0.05 in statistical evaluations. Normal distribution of data was determined with Shapiro-Wilk test. Paired t-test was used in the statistical analysis of normally distributed data. Comparing AST, ALT, GGT, LDH and CK values of boxers before and after the training, it has been observed that there is a significant increase in AST, LDH and CK values after the training (p<0.05) and significant decrease in GGT values (p<0.05) while there is no significant difference in ALT values (p>0.05). In conclusion, it has been determined that sparring training model applied to boxers affects liver enzyme levels in them and significant increases occur in CK and LDH. More comprehensive prospective studies are required in order to establish the possible damage of punches to the liver region on liver in boxers.

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Impact of Three Consecutive Days of Endurance Training Under Hypoxia on Muscle Damage and Inflammatory Responses

Frontiers in Sports and Active Living ◽

10.3389/fspor.2021.663095 ◽

2021 ◽

Vol 3 ◽

Author(s):

Daichi Sumi ◽

Keiichi Yamaguchi ◽

Kazushige Goto

Keyword(s):

Endurance Training ◽

Muscle Damage ◽

Inflammatory Responses ◽

Venous Blood ◽

High Sensitivity ◽

Knee Extension ◽

Voluntary Contraction ◽

Significant Difference ◽

Fraction Of Inspired Oxygen ◽

And Performance

Purpose: The purpose of this study was to determine the effect of 3 consecutive days of endurance training under hypoxia on muscle damage, inflammation, and performance responses.Methods: Nine active healthy males completed two trials in different periods, consisting of either 3 consecutive days of endurance training under hypoxia [fraction of inspired oxygen (Fio2): 14.5%, HYP] or normoxia (Fio2: 20.9%, NOR). They performed daily 90-min sessions of endurance training consisting of high-intensity endurance interval pedaling [10 × 4-min pedaling at 80% of maximal oxygen uptake (V˙o2max) with 2 min of active rest at 30% of V˙o2max] followed by 30-min continuous pedaling at 60% of V˙o2max during 3 consecutive days (days 1–3). Venous blood sample, muscular performance of lower limb, and score of subjective feelings were determined every morning (days 1–4) to evaluate muscle damage and inflammation. On day 4, subjects performed an incremental exercise test (IET) to evaluate the performance response.Results: Pedaling workload during daily endurance training was significantly lower in the HYP trial (interval exercise: 166 ± 4 W) than in the NOR trial (194 ± 8 W; P < 0.0001). Serum creatine kinase (CK) and high-sensitivity C-reactive protein (hsCRP) concentrations did not significantly change during days 1–4 in either trial. Maximal voluntary contraction (MVC) of knee extension (P < 0.0001) and drop jump (DJ) index (P = 0.004) were significantly decreased with training in both trials, with no significant difference between trials. The muscle soreness and fatigue scores significantly increased in both trials (P < 0.0001). However, the HYP trial showed a significantly lower score of fatigue on day 4 compared with the NOR trial (P = 0.004). Maximal aerobic power output during IET on day 4 did not significantly differ between trials.Conclusion: Three consecutive days of endurance training under hypoxia induced comparable levels of muscle damage, inflammation, and performance responses compared with the same training under normoxia.

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Vasopressin serum levels in patients with severe brain lesions and in brain-dead patients

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2004000200007 ◽

2004 ◽

Vol 62 (2a) ◽

pp. 226-232 ◽

Cited By ~ 9

Author(s):

Eliane de Araújo Cintra ◽

Jayme Antunes Maciel Jr ◽

Sebastião Araújo ◽

Margaret de Castro ◽

Edna Freitas Martins ◽

...

Keyword(s):

Healthy Volunteers ◽

Venous Blood ◽

Serum Levels ◽

Brain Lesions ◽

Control Group ◽

Open Label ◽

Significant Statistical Difference ◽

Significant Difference ◽

Brain Dead ◽

Trial Setting

INTRODUCTION: Patients with severe brain lesions (SBL) and brain-dead patients (BD) frequently present with vasopressin (AVP) secretion disorders. OBJECTIVE: To evaluate AVP serum levels in SBL and BD patients. DESIGN: Prospective, open label, observational trial. SETTING: A general teaching hospital. METHOD: Three groups of adult subjects (age> 18y) of both sexes were included in this study: control group: 29 healthy volunteers; SBL group: 17 patients with Glasgow Coma Scale (GCS)< 8; and BD group: 11 brain-dead patients. Samples of venous blood were collected in the morning at rest from healthy volunteers and at 8 hourly intervals over a period of 24h from SBL and BD patients for AVP determinations. Concomitantly, some clinical and laboratorial variables were also recorded. RESULTS: AVP serum levels (pg/ml) were [mean (SD); median]: control [2.2(1.1); 2.0]; SBL [5.7(6.3); 2.9]; and BD [2.6(1.0); 2.8]. AVP serum levels varied greatly in SBL patients, but without statistically significant difference in relation to the other groups (p=0.06). Hypotension (p=0.02), hypernatremia (p=0.0001), serum hyperosmolarity (p=0.0001) and urinary hypoosmolarity (p=0.003) were outstanding in BD patients when compared with SBL. CONCLUSIONS: The AVP serum levels did not demonstrate significant statistical difference between the groups, only showing a greater variability in SBL patients (manifested as serum spike levels). Hypernatremia and hyperosmolarity were present in BD patients, indicating a failure of the hypothalamic-pituitary system in AVP production and release.

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Auditory Evoked Potential Mismatch Negativity in Normal-Hearing Adults

International Archives of Otorhinolaryngology ◽

10.1055/s-0036-1586734 ◽

2016 ◽

Vol 21 (03) ◽

pp. 232-238 ◽

Cited By ~ 3

Author(s):

Laura Schwade ◽

Dayane Didoné ◽

Pricila Sleifer

Keyword(s):

Mismatch Negativity ◽

Statistical Difference ◽

Normal Hearing ◽

Auditory Evoked Potential ◽

Female Group ◽

Cross Sectional ◽

Significant Statistical Difference ◽

Left Handed ◽

Significant Difference ◽

The Right

Introduction Mismatch Negativity (MMN) corresponds to a response of the central auditory nervous system. Objective The objective of this study is to analyze MMN latencies and amplitudes in normal-hearing adults and compare the results between ears, gender and hand dominance. Methods This is a cross-sectional study. Forty subjects participated, 20 women and 20 men, aged 18 to 29 years and having normal auditory thresholds. A frequency of 1000Hz (standard stimuli) and 2000Hz (deviant stimuli) was used to evoked the MMN. Results Mean latencies in the right ear were 169.4ms and 175.3ms in the left ear, with mean amplitudes of 4.6µV in the right ear and 4.2µV in the left ear. There was no statistically significant difference between ears. The comparison of latencies between genders showed a statistically significant difference for the right ear, being higher in the men than in women. There was no significant statistical difference between ears for both right-handed and left-handed group. However, the results indicated that the latency of the right ear was significantly higher for the left handers than the right handers. We also found a significant result for the latency of the left ear, which was higher for the right handers. Conclusion It was possible to obtain references of values for the MMN. There are no differences in the MMN latencies and amplitudes between the ears. Regarding gender, the male group presented higher latencies in relation to the female group in the right ear. Some results indicate that there is a significant statistical difference of the MMN between right- and left-handed individuals.

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Effect of the period of extrinsic mechanical compression following sclerotherapy in veins in rabbit ears

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000300003 ◽

2011 ◽

Vol 26 (3) ◽

pp. 165-173 ◽

Cited By ~ 2

Author(s):

Cláudio Santana Ivo ◽

Marcelo Braga Ivo ◽

Paulo Guilherme de Oliveira Salles ◽

Ricardo Costa Val do Rosário ◽

Tarcizo Afonso Nunes

Keyword(s):

Blood Vessels ◽

Venous Blood ◽

Mechanical Compression ◽

Vein Thrombosis ◽

Dorsal Vein ◽

Venous Compression ◽

Significant Difference ◽

Blood Clots ◽

The Right ◽

Rabbit Ears

PURPOSE: Research whether a post-sclerotherapy venous compression period of up to 120 hours is sufficient to avoid reperfusion in treated veins; whether there is a relationship between the inflammatory intensity in venous walls and adjacent tissue and the size of venous thrombosis; whether the intensity of the post-sclerotherapy inflammation varies with the period of compression; whether there is a relationship between the presence of hemosiderin in the tissues adjacent to the sclerosing blood vessels and venous blood clots. METHODS: Twenty eight rabbits, all male, were utilized, distributed into four groups (0, 24, 72 and 120). All the animals were administered with 0.25 ml of 1% polidoconal solution and, as a control, 0.25 ml of 0.9% sodium chloride solution in the marginal dorsal vein of the right and left ears, respectively. Mechanical compression was applied to the perfused stretch of the vein, except for the animals in group 0. The period of compression varied from 0 to 120 hours in the groups. An anatomopathological examination of the section of the right and left marginal dorsal veins of all the animals was conducted. RESULTS: There was no significant difference among the various compression periods, both in terms of the degree of vein thrombosis and in the inflammatory intensity in both ears of the various groups. A positive and significant correlation was observed between the inflammatory intensity and the size of the thrombus and in the occurrence of thrombi and hemosiderin. CONCLUSIONS: A compression period of up to 120 hours is not sufficient to prevent reperfusion in sclerosing blood vessels. The intensity of tissue inflammation is related to the size of the thrombus, but not to the compression period. The presence of hemosiderin in the tissues adjacent to the vessels subjected to sclerosis is related to the presence of venous coagulation.

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Clinical verification of vimentin/EpCAM immunolipid magnetic sorting system in monitoring CTCs in arterial and venous blood of advanced tumor

Journal of Nanobiotechnology ◽

10.1186/s12951-021-00929-x ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Yan Liu ◽

Qiuying Li ◽

Tingsong Chen ◽

Tianhao Shen ◽

Xufeng Zhang ◽

...

Keyword(s):

Statistical Significance ◽

Venous Blood ◽

Gene Mutations ◽

Dominant Factor ◽

Positive Group ◽

Arterial Blood ◽

Significant Difference ◽

Targeted Drug ◽

Sorting System ◽

Advanced Tumors

Abstract Background Circulating tumor cells (CTCs) are the dominant factor leading to tumor metastasis. This study aims to investigate the effect of disparate sources of CTCs on the treatment and prognosis of patients with advanced tumors by analyzing the number and gene mutations change of CTCs in arterial and venous blood in patients with advanced tumors. Results A CTCs sorting system was constructed based on Vimentin-immunolipid magnetic balls (Vi-IMB) and EpCAM immunolipid magnetic balls (Ep-IMB). Results showed that the prepared Ep-IMB and Vi-IMB had lower cytotoxicity, better specificity and sensitivity. The number of arterial CTCs was higher than that of venous CTCs, with a statistically significant difference (P < 0.05). Moreover, the prognosis of the low positive group of total CTCs in arterial blood and venous blood was higher than that of the high positive group, with a statistical significance (P < 0.05). The genetic testing results showed that the targeted drug gene mutations in tissues, arterial CTCs and venous CTCs showed a complementary trend, indicating that there was heterogeneity among different tumor samples. Conclusions CTCs in blood can be efficiently captured by the CTCs sorting system based on Vi-LMB/Ep-LMB, and CTCs detection in arterial blood can be utilized to more accurately evaluate the prognosis and predict postoperative progress. It is further confirmed that tumor samples from disparate sources are heterogeneous, providing a reference basis for gene mutation detection before clinical targeted drug treatment, and the detection of CTCs in arterial blood has more potential clinical application value. Trial registration: The Ethics Committee of Putuo Hospital, PTEC-A-2019-18-1. Registered 24 September 2019. Graphic abstract

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