scholarly journals Impact of Three Consecutive Days of Endurance Training Under Hypoxia on Muscle Damage and Inflammatory Responses

2021 ◽  
Vol 3 ◽  
Author(s):  
Daichi Sumi ◽  
Keiichi Yamaguchi ◽  
Kazushige Goto
Keyword(s):  
Endurance Training ◽  
Muscle Damage ◽  
Inflammatory Responses ◽  
Venous Blood ◽  
High Sensitivity ◽  
Knee Extension ◽  
Voluntary Contraction ◽  
Significant Difference ◽  
Fraction Of Inspired Oxygen ◽  
And Performance

Purpose: The purpose of this study was to determine the effect of 3 consecutive days of endurance training under hypoxia on muscle damage, inflammation, and performance responses.Methods: Nine active healthy males completed two trials in different periods, consisting of either 3 consecutive days of endurance training under hypoxia [fraction of inspired oxygen (Fio2): 14.5%, HYP] or normoxia (Fio2: 20.9%, NOR). They performed daily 90-min sessions of endurance training consisting of high-intensity endurance interval pedaling [10 × 4-min pedaling at 80% of maximal oxygen uptake (V˙o2max) with 2 min of active rest at 30% of V˙o2max] followed by 30-min continuous pedaling at 60% of V˙o2max during 3 consecutive days (days 1–3). Venous blood sample, muscular performance of lower limb, and score of subjective feelings were determined every morning (days 1–4) to evaluate muscle damage and inflammation. On day 4, subjects performed an incremental exercise test (IET) to evaluate the performance response.Results: Pedaling workload during daily endurance training was significantly lower in the HYP trial (interval exercise: 166 ± 4 W) than in the NOR trial (194 ± 8 W; P < 0.0001). Serum creatine kinase (CK) and high-sensitivity C-reactive protein (hsCRP) concentrations did not significantly change during days 1–4 in either trial. Maximal voluntary contraction (MVC) of knee extension (P < 0.0001) and drop jump (DJ) index (P = 0.004) were significantly decreased with training in both trials, with no significant difference between trials. The muscle soreness and fatigue scores significantly increased in both trials (P < 0.0001). However, the HYP trial showed a significantly lower score of fatigue on day 4 compared with the NOR trial (P = 0.004). Maximal aerobic power output during IET on day 4 did not significantly differ between trials.Conclusion: Three consecutive days of endurance training under hypoxia induced comparable levels of muscle damage, inflammation, and performance responses compared with the same training under normoxia.

scholarly journals Investigation of the effect of supramaximal eccentric contractions on muscle damage and recovery between the dominant and non-dominant arm

2019 ◽  
Vol 23 (6) ◽  
pp. 306-312
Author(s):  
A. Yapıcı ◽  
H.B. Yalçın
Keyword(s):  
Muscle Damage ◽  
Male Volunteer ◽  
Statistical Significance ◽  
Venous Blood ◽  
Training Data ◽  
Eccentric Contractions ◽  
Significant Statistical Difference ◽  
Research Material ◽  
Significant Difference ◽  
The Right

Purpose: This research aimed to examine the effects of supramaximal eccentric contractions on the damage of muscle and recovery between the dominant and non-dominant arm. The study was participated by ten male volunteer students who do not have any musculoskeletal and metabolic compliant. This research designed as a cross-over type research. Material: Venous blood samples were drawn before the bout, immediately after the bout one min., moreover, at the 6th, 24th, 48th, and 72nd hours after training. Data were analyzed using the SPSS 21.0 statistical software package. After exercise, the non-parametric Wilcoxon Signed Ranks Test was used to decide the dominant and non-dominant arm influence. Statistical significance for all analyses was accepted at p< 0,05. Results: There was a significant statistical difference in the right, and left arm Creatine Kinase (CK) values at the time when exercise start, 48th and 72nd hours (p<0,05). There was no statistically significant difference in right and left arm Lactate Dehydrogenase values after exercise (p>0,05). Conclusions: There was an increase in muscle damage at 24th and 48th hours after supramaximal eccentric exercise, and it was turned back the start level at 72nd hours. While the arm which is non-dominant recovery is faster than the dominant arm.

scholarly journals Effect of Sparring Training Model on Liver Enzymes and Muscle Damage in Boxers

2019 ◽  
Vol 7 (3S) ◽  
pp. 114
Author(s):  
Ömer Kaynar
Keyword(s):  
Muscle Damage ◽  
Liver Enzymes ◽  
Venous Blood ◽  
Training Model ◽  
Acute Effect ◽  
Main Theme ◽  
Significance Level ◽  
Significant Difference ◽  
Before And After ◽  
Liver Region

The purpose of this study is to examine the acute effect of sparring training model on liver enzymes and muscle damage in boxers.14 voluntary athletes engaged in box (23.71±1.44 years of age; 167±5.89 cm; 62.05±8.89 kg) participated in the study. Sparring training model composed of warm-up (25 minutes), main part (30 minutes) and cool-down (15 minutes) exercises was applied to the boxers. The main theme of the study is composed of the free sub-stroke sparring training model in the liver region. Sparring training applied in the liver region and consisting of 3-minute time periods was repeated 3 times with a 1-minute interval as in the punch bag training. In venous blood samples taken from the study group before and after sparring training, AST, ALT and GGT enzyme activities and CK and LDH levels were analyzed with the spectrophotometric method in Beckman Coulter AU 5800 auto analyzer. Data were analyzed in SPSS 22.0 package program. The significance level was accepted as p<0.05 in statistical evaluations. Normal distribution of data was determined with Shapiro-Wilk test. Paired t-test was used in the statistical analysis of normally distributed data. Comparing AST, ALT, GGT, LDH and CK values of boxers before and after the training, it has been observed that there is a significant increase in AST, LDH and CK values after the training (p<0.05) and significant decrease in GGT values (p<0.05) while there is no significant difference in ALT values (p>0.05). In conclusion, it has been determined that sparring training model applied to boxers affects liver enzyme levels in them and significant increases occur in CK and LDH. More comprehensive prospective studies are required in order to establish the possible damage of punches to the liver region on liver in boxers.

Comparison of lipid and non-lipid markers of atherosclerosis in arterial versus venous blood

2003 ◽  
Vol 40 (3) ◽  
pp. 286-288 ◽  
Author(s):  
Bruce Palmer ◽  
Linley E Watson ◽  
Mark W Riggs ◽  
Mark Lawrence ◽  
Clinton E Baisden ◽  
...  
Keyword(s):  
Particle Density ◽  
Venous Blood ◽  
Hdl Cholesterol ◽  
High Sensitivity ◽  
Heart Catheterization ◽  
Blood Concentrations ◽  
Reactive Protein ◽  
Amyloid A ◽  
Left Heart Catheterization ◽  
Significant Difference

Background: Early studies have suggested that there may be differences in the concentration of lipoprotein particles and their associated apolipoproteins in arterial and venous blood and that this gradient might explain a proclivity to develop atherosclerotic lesions. The aim of this study was to use current methods of analysis to determine levels of these components, including particle densities and several common inflammatory markers in arterial and venous blood. Methods: Samples of arterial and venous blood were obtained nearly simultaneously in 26 patients undergoing right and left heart catheterization. Analyses were performed using enzymatic, immunoturbidimetric and ultracentrifugation assays. Results: Data obtained for total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, HDL and LDL particle density, high sensitivity C-reactive protein, serum amyloid-A and apoprotein B-100 concentrations in arterial and venous blood did not demonstrate any significant difference in the means. Conclusion: Arterial and venous blood can be used interchangeably to study the effect of blood concentrations of common soluble surrogate markers of atherosclerosis.

Effect of a prior bout of preconditioning exercise on muscle damage from downhill walking

2015 ◽  
Vol 40 (3) ◽  
pp. 274-279 ◽  
Author(s):  
Sumiaki Maeo ◽  
Yusuke Ochi ◽  
Masayoshi Yamamoto ◽  
Hiroaki Kanehisa ◽  
Kazunori Nosaka
Keyword(s):  
Protective Effect ◽  
Muscle Damage ◽  
Muscle Soreness ◽  
Knee Extension ◽  
Voluntary Contraction ◽  
Control Group ◽  
Analog Scale ◽  
Downhill Walking ◽  
Plasma Creatine Kinase ◽  
Minor Muscle

This study investigated whether reduced-duration downhill walking (DW) would confer a protective effect against muscle damage induced by a subsequent bout of longer duration DW performed 1 week or 4 weeks later. Healthy young adults were allocated to a control or one of the preconditioning exercise (PRE-1wk or PRE-4wk) groups (10 men and 4 women per group). PRE-1wk and PRE-4wk groups performed 20-min DW (–28% slope, 5 km/h, 10% body mass added to a backpack) 1 week and 4 weeks before 40-min DW, respectively, and the control group performed 40-min DW only. Maximal voluntary contraction (MVC) knee extension torque, plasma creatine kinase (CK) activity, and muscle soreness (100-mm visual analog scale) were measured before, immediately after, and 24, 48, and 72 h after DW, and the changes in these variables were compared among groups. The control group showed symptoms of muscle damage (e.g., prolonged decrease in MVC: –14% ± 10% at 48 h post-DW) after 40-min DW. Changes in all variables after 40-min DW of PRE-1wk and PRE-4wk groups were 54%–61% smaller (P < 0.05) than the control group, without significant differences between PRE-1wk and PRE-4wk groups for MVC and plasma CK activity. Importantly, changes after the preconditioning exercise (20-min DW) were 67%–69% smaller (P < 0.05) than those after the 40-min DW of the control group. These findings suggest that 20-min DW resulting in minor muscle damage conferred a protective effect against subsequent 40-min DW, and its effect could last for more than 4 weeks.

scholarly journals Inflammatory Cytokines and Sarcopenia in Iranian Adults-results from SARIR Study

2021 ◽  
Author(s):  
Farzaneh Asoudeh ◽  
Fatemeh Dashti ◽  
Shima Raeesi ◽  
Ramin Heshmat ◽  
Mohammad Bidkhori ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Inflammatory Cytokines ◽  
High Sensitivity ◽  
Inflammatory Biomarkers ◽  
P Value ◽  
Cluster Sampling ◽  
European Working ◽  
Significant Difference ◽  
Hs Crp ◽  
And Performance

Abstract Background Some studies emphasize the effects of inflammatory cytokines in reducing muscle mass and muscle strength and performance. This study aimed to compare pro-inflammatory cytokines in sarcopenic and non-sarcopenic subjects. Method The present study used data from the "Sarcopenia and its Determinants Among Iranian Elders" (SARIR) study. Three hundred men and women aged ≥ 55 years old who lived in the 6th district of Tehran were selected using random cluster sampling. We measured all components by using standard protocols, in addition we defined sarcopenia based on former and new European Working Group on Sarcopenia in Older People (EWGSOP) guidelines. A fasting blood sample was taken from each participant to measure serum high-sensitivity C-reactive protein (hs- CRP), Interleukin 6 (IL-6), and tumor necrosis factor α (TNFα). Results Mean age and body mass index (BMI) of study participants were 66.7 ± 7.7 years and 27.3 ± 4.2 kg/m2, respectively. Thirty-one participants of the present study had the criteria of EWGSOP2-sarcopenia. A statistically significant difference was seen between normal and abnormal groups of muscle strength in hs-CRP (p-value =0.04). Furthermore, we did not observe a remarkable association between inflammatory biomarkers including IL-6, TNF-α and hs-CRP and risk of sarcopenia even after controlling for plausible confounders (OR: 1.15; 95% CI 0.31-4.28, OR 0.68; 95% CI 0.17-2.77 and OR 2.39; 95% CI 0.87-6.55). Conclusion We found that inflammatory biomarkers level was not considerably associated with odds of sarcopenia. Lack of correlation between inflammatory cytokines and sarcopenia could be due to participant’s age and genetic. Future studies are required to confirm these findings.

scholarly journals Flavanol-Rich Cacao Mucilage Juice Enhances Recovery of Power but Not Strength from Intensive Exercise in Healthy, Young Men

Sports ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 159 ◽  
Author(s):  
Paul Morgan ◽  
Paola Wollman ◽  
Sarah Jackman ◽  
Joanna Bowtell
Keyword(s):  
Venous Blood ◽  
Maximum Voluntary Contraction ◽  
Knee Extensor ◽  
Knee Extension ◽  
Voluntary Contraction ◽  
Post Exercise ◽  
Intensive Exercise ◽  
Counter Movement Jump ◽  
Knee Extension Exercise ◽  
Time Point

(1) Background: Mucilage within cacao pods contains high levels of polyphenols. We investigated whether consumption of cacao juice enhances the recovery of muscle function following intensive knee extension exercise. (2) Methods: Ten recreationally active males completed two trials of 10 sets of 10 single leg knee extensions at ~80% one repetition maximum. Participants consumed each supplement (ZumoCacao® juice, CJ or a dextrose drink, PL) for 7 days prior to and 48 h post exercise. Knee extension maximum voluntary contraction (MVC) and a counter movement jump (CMJ) were performed at baseline, immediately, 24 h, and 48 h post-exercise. Venous blood samples were collected at each time point and analyzed for indices of inflammation, oxidative damage, and muscle damage. (3) Results: CMJ height recovered faster with CJ at 24 h and 48 h post-exercise (p < 0.05), but there was no effect of CJ on recovery of MVC (both p > 0.05). There was also no effect of the trial on any blood markers (all p > 0.05). (4) Conclusions: Supplementation with CJ for 7 days prior to and 2 days after intensive knee extensor exercise improved functional recovery as shown by an improved recovery of CMJ up to 48 h post-exercise. However, the precise mechanism of action is unclear and requires further investigation.

Application of liquid biopsy to uncover critical cancer insights using the GeneReader NGS system.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e13102-e13102
Author(s):  
Luisa Andruzzi
Keyword(s):  
Liquid Biopsy ◽  
High Sensitivity ◽  
Allelic Frequency ◽  
Reference Standards ◽  
Great Promise ◽  
Fully Integrated ◽  
Circulating Free Dna ◽  
Significant Difference ◽  
Sequencing Chemistry ◽  
And Performance

e13102 Background: NGS studies of cancer are primarily based on studies of FFPE samples, the use of which is limited by surgical feasibility, tissue availability and patient preference. An emerging field of study is focused on circulating-free DNA (cfDNA), sometimes termed “Liquid Biopsy”. While holding great promise as the future of cancer understanding and management, the liquid biopsy field is still nascent, lacking an integrated NGS workflow solution with proven performance. Methods: QIAGEN is an established expert in the field of sample preparation for liquid biopsy. The combination of the QIAamp Circulating Nucleic Acid Kit, the GeneReader NGS System using a new proprietary sequencing chemistry, and an optimized bioinformatics solution, provides the first fully integrated workflow specifically designed for analyzing cfDNA. A set of liquid biopsy samples from non-small cell lung cancer (NSCLC) patients and cfDNA reference standards (Horizon), with allelic frequency down to 1%, were used for this study. cfDNA was extracted from the liquid biopsy samples, and run through the QIAGEN GeneReader workflow. Variants were analysed using the QIAGEN Clinical Insight (QCI™) Analyze for liquid biopsy. Results: Expected mutations in all genes (EGFR, KRAS, NRAS, PI3KCA) were identified and verified, at 100% concordance with reported in ddPCR studies. Both single nucleotide and insertion-deletion variants were correctly detected down to a 1% allele frequency threshold which was reproducible between repeat runs. No significant difference was observed between cfDNA reference standards and clinical liquid biopsy samples. Conclusions: These data exhibit high sensitivity and performance consistency of liquid biopsy analysis with the GeneReader system. This study systematically demonstrates the accuracy of a cfDNA assay combined with the robustness of a fully integrated NGS system. As such, laboratories interested in conducting liquid biopsy studies can leverage this study and the system to implement an NGS workflow.

scholarly journals Effect of elastic-band exercise on muscle damage and inflammatory responses in Taekwondo athletes

2015 ◽  
Vol 21 (4) ◽  
pp. 297-301 ◽  
Author(s):  
Keivan Gadruni ◽  
Hemn Mahmmadpour ◽  
Mohammad Gadruni
Keyword(s):  
Muscle Damage ◽  
Inflammatory Responses ◽  
Elastic Band ◽  
Post Exercise ◽  
Ldh Activity ◽  
Elastic Resistance ◽  
Significant Difference ◽  
Leukocyte Counts ◽  
Exercise Induced ◽  
Progressive Resistance

INTRODUCTION: Elastic bands offer variable elastic resistance (ER) throughout a range of motion and their incorporation with exercise movements has been used for variable strength training and rehabilitation purposes. Objective: Investigate the effect of acute bout of progressive elastic-band exercise on muscle damage and inflammatory response in Taekwondo athletes (TKD) compared with untrained ones.METHODS: Fourteen (TKD, n = 7 and untrained, n = 7) men performed 3 sets of progressive resistance elastic exercise. Blood samples were taken pre-exercise and also immediately and 24h post exercise. Delayed onset muscle soreness (DOMS), creatine kinase (CK) and lactate dehydrogenase (LDH) activity, total leukocyte counts, interleukin-6 and C-reactive protein (CRP) were analyzed.RESULTS: Only DOMS increased in untrained group, but elevation of DOMS was observed in both groups (TKD and untrained) at 24h after exercise (p<0.05). CK and LDH activity increased in both groups significantly. Also TKD group only showed CK increasing 24h post exercise (p<0.05). Total circulating leukocyte counts increased immediately in post exercise experiments and decreased in 24h ones in both groups (p<0.05). Serum IL-6 immediately increased in both groups and 24h post exercises but there was no significant difference between immediate and 24h post exercise experiments in TKD group. Furthermore, CRP just increased 24h after exercise in both groups (p<0.05).CONCLUSION: Progressive resistance elastic exercise induced muscle damage and inflammation in TKD athletes, but also had smaller changes in comparison with untrained group and other forms of exercise.

Markers of Hemostatic Activation in Affected Coronary Arteries during Angioplasty

1994 ◽  
Vol 72 (05) ◽  
pp. 672-675 ◽  
Author(s):  
Nicolas W Shammas ◽  
Michael J Cunningham ◽  
Richard M Pomearntz ◽  
Charles W Francis
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Venous Blood ◽  
Balloon Inflation ◽  
Blood Samples ◽  
Hemostatic System ◽  
Significant Difference ◽  
Hemostatic Markers ◽  
Artery Disease ◽  
High Doses

SummaryTo characterize the extent of early activation of the hemostatic system following angioplasty, we obtained blood samples from the involved coronary artery of 11 stable angina patients during the procedure and measured sensitive markers of thrombin formation (fibrino-peptide A, prothrombin fragment 1.2, and soluble fibrin) and of platelet activation ((3-thromboglobulin). Levels of hemostatic markers in venous blood obtained from 14 young individuals with low pretest probability for coronary artery disease were not significantly different from levels in venous blood or intracoronary samples obtained prior to angioplasty. Also, there was no translesional (proximal and distal to the lesion) gradient in any of the hemostatic markers before or after angioplasty in samples obtained between 18 and 21 min from the onset of the first balloon inflation. Furthermore, no significant difference was noted between angioplasty and postangioplasty intracoronary concentrations. We conclude that intracoronary hemostatic activation does not occur in the majority of patients during and immediately following coronary angioplasty when high doses of heparin and aspirin are administered.

Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

2018 ◽  
Vol 1 (2) ◽  
pp. 114
Author(s):  
Wahdaniah Wahdaniah ◽  
Sri Tumpuk
Keyword(s):  
Screening Test ◽  
Venous Blood ◽  
The Body ◽  
Ethylene Diamine ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ethylene Diamine Tetra Acetate ◽  
The Difference ◽  
Index Value

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk  K2EDTA dan  K3EDTA. K3EDTA  biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

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