scholarly journals Critical Issues in Successful Production of Skin Substitutes for Wound Healing

2018 ◽  
Vol 4 ◽  
pp. 10
Author(s):  
Hojjat Naderi-Meshkin ◽  
Raheleh Amirkhah ◽  
Asieh Heirani-Tabasi ◽  
Muhammad Irfan-maqsood
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Healing Process ◽  
Basic Knowledge ◽  
Skin Substitutes ◽  
Commercial Development ◽  
Wide Range ◽  
Critical Issues ◽  
Successful Production

Novel findings on fabrication techniques for bioactive materials, discovering further basic knowledge about wound healing process, and availability of stem cells as alternative candidate for differentiated cells have highly encouraged scientists for developing new bioengineered skin substitutes (BSS) that offer an effective remedy for a specific wound type. However, technical, clinical, legislative and economic reasons hamper wide-spread commercialization and clinical translation of BSS. Among the various types of strategies that target skin repair and regeneration, tissue engineering with stem cells is most promising route. Tissue engineering by cooperation of several disciplines forms a context on which the commercial development of BSS is possible to provide benefits for patients who currently have limited or no cure options. The principles of tissue engineering are to initiate cell cultures in vitro, grow them in monolayer or on porous scaffolds and transplant the composite into a patient with a specific wound indication in vivo. The potential for creating of custom-designed biomaterials and availability of stem cells from either autologous or allogenic sources have helped to produce novel innovative BSS. Currently, wide range of skin substitutes are already being fabricated for clinical use in different wound indications but not yet definitively established. Therefore, many novel engineered constructs might be fabricated in the future. In this review, we describe the progress that has been made to date in the field of skin substitutes and the critical issues that are still hindering successful production and bench to bedside translation of BSS and restricting the availability of these innovative therapeutic constructs. Integrity of the science and technology, interdisciplinary expertise collaborations, and early interaction with regulatory entities such as Food and Drug Administration (FDA) and European Medicines Agency (EMA), together with other critical determinants, is vital to the successful commercialization of tissue engineering products into the marketplace/clinic.

scholarly journals Immunomodulation of Skin Repair: Cell-Based Therapeutic Strategies for Skin Replacement (A Comprehensive Review)

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 118
Author(s):  
Shima Tavakoli ◽  
Marta A. Kisiel ◽  
Thomas Biedermann ◽  
Agnes S. Klar
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Skin Wound ◽  
Skin Tissue ◽  
Diabetic Patients ◽  
Specific Cell ◽  
Skin Substitutes ◽  
Skin Wound Healing ◽  
Skin Tissue Engineering

The immune system has a crucial role in skin wound healing and the application of specific cell-laden immunomodulating biomaterials emerged as a possible treatment option to drive skin tissue regeneration. Cell-laden tissue-engineered skin substitutes have the ability to activate immune pathways, even in the absence of other immune-stimulating signals. In particular, mesenchymal stem cells with their immunomodulatory properties can create a specific immune microenvironment to reduce inflammation, scarring, and support skin regeneration. This review presents an overview of current wound care techniques including skin tissue engineering and biomaterials as a novel and promising approach. We highlight the plasticity and different roles of immune cells, in particular macrophages during various stages of skin wound healing. These aspects are pivotal to promote the regeneration of nonhealing wounds such as ulcers in diabetic patients. We believe that a better understanding of the intrinsic immunomodulatory features of stem cells in implantable skin substitutes will lead to new translational opportunities. This, in turn, will improve skin tissue engineering and regenerative medicine applications.

scholarly journals Cellular human tissue-engineered skin substitutes investigated for deep and difficult to heal injuries

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Álvaro Sierra-Sánchez ◽  
Kevin H. Kim ◽  
Gonzalo Blasco-Morente ◽  
Salvador Arias-Santiago
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Human Cell ◽  
Chronic Wounds ◽  
Healing Process ◽  
Wound Healing Process ◽  
Future Perspectives ◽  
Skin Substitutes ◽  
Cell Potential ◽  
Engineered Skin Substitutes

AbstractWound healing is an important function of skin; however, after significant skin injury (burns) or in certain dermatological pathologies (chronic wounds), this important process can be deregulated or lost, resulting in severe complications. To avoid these, studies have focused on developing tissue-engineered skin substitutes (TESSs), which attempt to replace and regenerate the damaged skin. Autologous cultured epithelial substitutes (CESs) constituted of keratinocytes, allogeneic cultured dermal substitutes (CDSs) composed of biomaterials and fibroblasts and autologous composite skin substitutes (CSSs) comprised of biomaterials, keratinocytes and fibroblasts, have been the most studied clinical TESSs, reporting positive results for different pathological conditions. However, researchers’ purpose is to develop TESSs that resemble in a better way the human skin and its wound healing process. For this reason, they have also evaluated at preclinical level the incorporation of other human cell types such as melanocytes, Merkel and Langerhans cells, skin stem cells (SSCs), induced pluripotent stem cells (iPSCs) or mesenchymal stem cells (MSCs). Among these, MSCs have been also reported in clinical studies with hopeful results. Future perspectives in the field of human-TESSs are focused on improving in vivo animal models, incorporating immune cells, designing specific niches inside the biomaterials to increase stem cell potential and developing three-dimensional bioprinting strategies, with the final purpose of increasing patient’s health care. In this review we summarize the use of different human cell populations for preclinical and clinical TESSs under research, remarking their strengths and limitations and discuss the future perspectives, which could be useful for wound healing purposes.

scholarly journals Outlook for Wound Healing Technologies (a Review)

2020 ◽  
Vol 10 (2) ◽  
pp. 130-136
Author(s):  
V. V. Chebotarev ◽  
Z. R. Khismatullina ◽  
L. K. Nasyrova
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Medical Science ◽  
Living Organism ◽  
Biological Tissues ◽  
Structural Level ◽  
Skin Substitutes ◽  
Wide Range ◽  
Artificial Extracellular Matrix ◽  
And Function

Tissue engineering is a medical science dealing with reproduction of biological tissues and organs. This area of medicine opens avenues for creation of organs and tissues using biomaterials and nanostructures to sustain their development, maintenance and function repair in a living organism. The scope of tissue engineering is an artificial recreation of tissues at the fi nest structural level. Prerequisite requirements are a cell source (a donor), artificial extracellular matrix and growth factor. The first organ, which was extracorporally created and successfully introduced in medical practice, is skin. Recent years have witnessed a major leap in 3D technology for reproduction of biological structures. Increasing attention is being paid towards controlled design and production of 2D–3D structures consisting of biological materials and viable cells, the procedure defined as bioproduction or bioprototyping. Skin substitutes obtained with the bioprototyping technology possess a wide range of medical applications, primarily to compensate for resident skin deficiency in wound healing.

Critical Issues in Successful Production of Skin Substitutes for Wound Healing

2016 ◽  
Vol 1 (2) ◽  
pp. 38 ◽  
Author(s):  
Hojjat Naderi-Meshkin ◽  
Raheleh Amirkhah ◽  
Asieh Heirani-Tabasi ◽  
Muhammad Irfan-Maqsood
Keyword(s):  
Wound Healing ◽  
Skin Substitutes ◽  
Critical Issues ◽  
Successful Production

scholarly journals Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hamed Nosrati ◽  
Reza Aramideh Khouy ◽  
Ali Nosrati ◽  
Mohammad Khodaei ◽  
Mehdi Banitalebi-Dehkordi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Tissue Regeneration ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
The Body ◽  
Key Factors ◽  
Potential Applications ◽  
Nanocomposite Scaffolds

AbstractSkin is the body’s first barrier against external pathogens that maintains the homeostasis of the body. Any serious damage to the skin could have an impact on human health and quality of life. Tissue engineering aims to improve the quality of damaged tissue regeneration. One of the most effective treatments for skin tissue regeneration is to improve angiogenesis during the healing period. Over the last decade, there has been an impressive growth of new potential applications for nanobiomaterials in tissue engineering. Various approaches have been developed to improve the rate and quality of the healing process using angiogenic nanomaterials. In this review, we focused on molecular mechanisms and key factors in angiogenesis, the role of nanobiomaterials in angiogenesis, and scaffold-based tissue engineering approaches for accelerated wound healing based on improved angiogenesis.

scholarly journals Effect of Amniotic Fluid Stem Cells and Amniotic Fluid Cells on the Wound Healing Process in a White Rat Model

2013 ◽  
Vol 40 (5) ◽  
pp. 496 ◽  
Author(s):  
Jung Dug Yang ◽  
Dong Sik Choi ◽  
Young Kyoo Cho ◽  
Taek Kyun Kim ◽  
Jeong Woo Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Amniotic Fluid ◽  
Rat Model ◽  
Healing Process ◽  
Wound Healing Process ◽  
Amniotic Fluid Stem Cells ◽  
Amniotic Fluid Cells

scholarly journals Nanostructured Fibers Containing Natural or Synthetic Bioactive Compounds in Wound Dressing Applications

Materials ◽  
2020 ◽  
Vol 13 (10) ◽  
pp. 2407 ◽  
Author(s):  
Alexa-Maria Croitoru ◽  
Denisa Ficai ◽  
Anton Ficai ◽  
Natalia Mihailescu ◽  
Ecaterina Andronescu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Electrospun Nanofibers ◽  
Natural Compounds ◽  
Electrospinning Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Skin Substitutes ◽  
Bioactive Constituents ◽  
Electrospinning Method

The interest in wound healing characteristics of bioactive constituents and therapeutic agents, especially natural compounds, is increasing because of their therapeutic properties, cost-effectiveness, and few adverse effects. Lately, nanocarriers as a drug delivery system have been actively investigated and applied in medical and therapeutic applications. In recent decades, researchers have investigated the incorporation of natural or synthetic substances into novel bioactive electrospun nanofibrous architectures produced by the electrospinning method for skin substitutes. Therefore, the development of nanotechnology in the area of dressings that could provide higher performance and a synergistic effect for wound healing is needed. Natural compounds with antimicrobial, antibacterial, and anti-inflammatory activity in combination with nanostructured fibers represent a future approach due to the increased wound healing process and regeneration of the lost tissue. This paper presents different approaches in producing electrospun nanofibers, highlighting the electrospinning process used in fabricating innovative wound dressings that are able to release natural and/or synthetic substances in a controlled way, thus enhancing the healing process.

scholarly journals Encapsulation of Adipose-Derived Mesenchymal Stem Cells in Calcium Alginate Maintains Clonogenicity and Enhances their Secretory Profile

2020 ◽  
Vol 21 (17) ◽  
pp. 6316
Author(s):  
Lucille Capin ◽  
Nacira Abbassi ◽  
Maëlle Lachat ◽  
Marie Calteau ◽  
Cynthia Barratier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Calcium Alginate ◽  
Healing Process ◽  
Spherical Particles ◽  
Differentiation Potential ◽  
Monolayer Cultures ◽  
The Impact

Adipose-derived mesenchymal stem cells (ASCs) are well known for their secretory potential, which confers them useful properties in cell therapy. Nevertheless, this therapeutic potential is reduced after transplantation due to their short survival in the human body and their migration property. This study proposes a method to protect cells during and after injection by encapsulation in microparticles of calcium alginate. Besides, the consequences of encapsulation on ASC proliferation, pluripotential, and secretome were studied. Spherical particles with a mean diameter of 500 µm could be obtained in a reproducible manner with a viability of 70% after 16 days in vitro. Moreover, encapsulation did not alter the proliferative properties of ASCs upon return to culture nor their differentiation potential in adipocytes, chondrocytes, and osteocytes. Concerning their secretome, encapsulated ASCs consistently produced greater amounts of interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) compared to monolayer cultures. Encapsulation therefore appears to enrich the secretome with transforming growth factor β1 (TGF-β1) and macrophage inflammatory protein-1β (MIP-1β) not detectable in monolayer cultures. Alginate microparticles seem sufficiently porous to allow diffusion of the cytokines of interest. With all these cytokines playing an important role in wound healing, it appears relevant to investigate the impact of using encapsulated ASCs on the wound healing process.

scholarly journals From Grafts to Human Bioengineered Vascularized Skin Substitutes

2020 ◽  
Vol 21 (21) ◽  
pp. 8197
Author(s):  
Wasima Oualla-Bachiri ◽  
Ana Fernández-González ◽  
María I. Quiñones-Vico ◽  
Salvador Arias-Santiago
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Chronic Wounds ◽  
Thermal Regulation ◽  
Skin Substitute ◽  
Skin Substitutes ◽  
Normal Wound Healing ◽  
Physiological Homeostasis ◽  
Promising Solution ◽  
Native Skin

The skin plays an important role in the maintenance of the human’s body physiological homeostasis. It acts as a coverage that protects against infective microorganism or biomechanical impacts. Skin is also implied in thermal regulation and fluid balance. However, skin can suffer several damages that impede normal wound-healing responses and lead to chronic wounds. Since the use of autografts, allografts, and xenografts present source limitations and intense rejection associated problems, bioengineered artificial skin substitutes (BASS) have emerged as a promising solution to address these problems. Despite this, currently available skin substitutes have many drawbacks, and an ideal skin substitute has not been developed yet. The advances that have been produced on tissue engineering techniques have enabled improving and developing new arising skin substitutes. The aim of this review is to outline these advances, including commercially available skin substitutes, to finally focus on future tissue engineering perspectives leading to the creation of autologous prevascularized skin equivalents with a hypodermal-like layer to achieve an exemplary skin substitute that fulfills all the biological characteristics of native skin and contributes to wound healing.

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