scholarly journals Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 6
Author(s):  
Putri Sri Hartini ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Post Test ◽  
Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Channa Striata ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.</p><p>Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.</p>

scholarly journals Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

2016 ◽  
Vol 49 (3) ◽  
pp. 125
Author(s):  
Gunawan Oentaryo ◽  
Istiati Istiati ◽  
Pratiwi Soesilawati
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Tissue Growth ◽  
Control Group ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Treatment Groups ◽  
Collagen Secretion ◽  
Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

scholarly journals EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Unsaturated Fat ◽  
Post Test ◽  
Channa Striata ◽  
The Mean

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

scholarly journals Extract of haruan (Channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Devintha Ayu Mellyana Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.</p>

scholarly journals Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 224
Author(s):  
Hardany Primarizky ◽  
Wiwik Misaco Yuniarti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Ellagic Acid ◽  
Healing Process ◽  
Control Group ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Significant Difference ◽  
Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05).  From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

scholarly journals The effect of papaya leaf extract (Carica papaya L) on healing process of buccal traumatic ulcer in wistar rats

2019 ◽  
Vol 1 (1) ◽  
pp. 15
Author(s):  
Afryla Femilian ◽  
Dewi Agustina ◽  
Goeno Subagyo
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Buccal Mucosa ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Healing Process ◽  
Herbal Medicines ◽  
Ethanolic Extract ◽  
Control Group ◽  
Wound Healing Process

Indonesians have been using herbal medicines for a long time to cure some illnesses. Carica papaya L is an example of an herb that contains papain enzymes, saponins, lysozymes, lipases, flavonoids, polyphenols and vitamin C. These  ingredients are believed to be beneficial for the wound healing process. The purpose of this study was to determine the effect of topical application of ethanolic extract of Carica papaya L to the healing process of rat mouth ulcer. Subjects were 32 Wistar rats divided into 2 groups (control group and treatment group), each group containing 16 rats. All subjects were made to suffer from ulcers using glacial acetyl acid applied for 40 seconds in the buccal mucosa. Treatment group was treated with papaya leaf ethanolic extract on ulcers twice a day using microbrush, while the ulcer in the control group was not treated with the extract. The ulcerated tissue was biopsied and stained with H&E. Observations were performed on the day 0, 3rd, 7th and 12th on HE slides. Data were observed by looking at three indicators of wound healing i.e. macrophage, angiogenesis and re-epithelisation. Number of macrophages and angiogenesis were analyzed using two-way ANOVA. Data of epithelial thickness were analyzed using Kruskal-Wallis test. The post hoc test in the treatment group and the control group on day 0 showed results of p = 1.00. On day 3, day 7 and day 12, the result of p was <0.05. In the treatment group on day 0 compared to the treatment group on the 3rd, 7th, 12th day, the results were p<0.05. In the treatment group on the 3rd day compared to the 7th and 12th days the results were p>0.05. The treatment group on the 7 day compared to the treatment group on the 12 day had p>0.05. In the control group, on day 0 compared to day 3, the results of p>0.05, while the control group day 0 with day 7, 12 had a result of p<0.05. The control group on the 3rd day was compared with the 7th day. The 12th also had a result of p<0.05. The comparison between the 7 day control groups showed p of >0.05. These data suggest that the papaya leaf ethanolic extract could accelerate the healing of oral ulcer on the buccal mucosa of wistar rats.

scholarly journals Ekstrakharuan(Channa striata)secara efektif menurunkan jumlah limfosit fase inflamasi dalam penyembuhan luka (Extract of haruan (Channa striata) decreases lymphocyte count in inflammatory phase of wound healing process effectively)

2014 ◽  
Vol 13 (3) ◽  
pp. 176
Author(s):  
Arisa Izzaty ◽  
Nurdiana Dewi ◽  
Dewi Indah Noviana Pratiwi
Keyword(s):  
Wound Healing ◽  
Lymphocyte Count ◽  
Healing Process ◽  
Simple Random Sampling ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Inflammatory Phase ◽  
Channa Striata

Haruan is river-water fish usually consumed by South Kalimantan people. Haruan extract contains albumin, Zn, Fe,Cu and unsaturated fatty acid which can act as antiinflammatory substances and accelarate wound healing. The aim ofthis study is to examine effectiveness of haruan extract on lymphocyte count in infammatory phase of wound healingprocess and compare it to ibuprofen. This study used true experimental method with post-test only control group designand simple random sampling. Samples were divided into 5 treatment groups:which were given the extract for 7 sevendays: 25% haruan extract group, 50% haruan extract group, 100% haruan extract group, negative control K(-) usingaquadest, positive control K(+) given ibuprofen suspension with dosage of 20mg/kg weight/day. Lymphocyte cells wereobserved using microscope and counted per field of view. Lymphocyte count reached its peak on day 5. Lymphocytecount means in each group were 4,2 ± 0,566; 1± 0,000; 3,8 ± 2,546; 7,1 ± 0,141; dan 7,5 ± 3,818 respectively. Two way ANOVA and post hoc LSD tests showed that lymphocyte count significantly decreased in 50% and 100% treatmentgroups (p<0,05) and has equal effect with ibuprofen in decreasing lymphocyte count. This study concluded that haruanextract significantly decreased lymphocyte count in wound healing process.

scholarly journals Extract of haruan (channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Devintha AM. Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.

scholarly journals Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 226
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p&lt;0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

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