Increased Stem Cell Adhesion on Carbon Nanotubes Grown from Anodized Titanium

2006 ◽  
Vol 951 ◽  
Author(s):  
Batur Ercan ◽  
Thomas Webster
Keyword(s):  
Carbon Nanotubes ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Chemical Vapor ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Human Mesenchymal Stem Cell ◽  
The Body ◽  
Differentiated Cells ◽  
Anodized Titanium

ABSTRACTStem cells are the source of all differentiated cells in the body. However, the stimuli that control stem cell differentiation into other cell types is still not well understood. In order to investigate this, novel carbon nanotubes (CNT's) were synthesized on anodized titanium substrates by chemical vapor deposition. Human mesenchymal stem cell (HMCS) adhesion was investigated on this nano-structured surface. The results showed significant evidence for the enhancement of HMSC adhesion on carbon nanotubes with respect to the template they were synthesized on, specifically, anodized titanium. Such evidence provides promise for the use of carbon nanotubes grown from anodized titanium to serve as novel stem cell differentiation devices.

scholarly journals Stem cell differentiation trajectories inHydraresolved at single-cell resolution

2018 ◽  
Author(s):  
Stefan Siebert ◽  
Jeffrey A. Farrell ◽  
Jack F. Cazet ◽  
Yashodara L. Abeykoon ◽  
Abby S. Primack ◽  
...  
Keyword(s):  
Nervous System ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Cell Lineage ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Differentiated Cells ◽  
Nervous System Function ◽  
Stem Cell Populations ◽  
Molecular Description

AbstractThe adultHydrapolyp continuously renews all of its cells using three separate stem cell populations, but the genetic pathways enabling homeostatic tissue maintenance are not well understood. We used Drop-seq to sequence transcriptomes of 24,985 singleHydracells and identified the molecular signatures of a broad spectrum of cell states, from stem cells to terminally differentiated cells. We constructed differentiation trajectories for each cell lineage and identified the transcription factors expressed along these trajectories, thus creating a comprehensive molecular map of all developmental lineages in the adult animal. We unexpectedly found that neuron and gland cell differentiation transits through a common progenitor state, suggesting a shared evolutionary history for these secretory cell types. Finally, we have built the first gene expression map of theHydranervous system. By producing a comprehensive molecular description of the adultHydrapolyp, we have generated a resource for addressing fundamental questions regarding the evolution of developmental processes and nervous system function.

Transcriptome Analysis During Normal Human Mesenchymal Stem Cell Differentiation

2014 ◽  
pp. 109-119
Author(s):  
Karina F. Bombonato-Prado ◽  
Adalberto L. Rosa ◽  
Paulo T. Oliveira ◽  
Janaína A. Dernowsek ◽  
Vanessa Fontana ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Transcriptome Analysis ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Mesenchymal Stem Cell Differentiation ◽  
Normal Human

scholarly journals Lineage-specific exosomes could override extracellular matrix mediated human mesenchymal stem cell differentiation

Biomaterials ◽  
2018 ◽  
Vol 182 ◽  
pp. 312-322 ◽  
Author(s):  
Karthikeyan Narayanan ◽  
Sundramurthy Kumar ◽  
Parasuraman Padmanabhan ◽  
Balazs Gulyas ◽  
Andrew C.A. Wan ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Mesenchymal Stem Cell Differentiation

Refined assessment of the impact of cell shape on human mesenchymal stem cell differentiation in 3D contexts

2019 ◽  
Vol 87 ◽  
pp. 166-176 ◽  
Author(s):  
Andrea C. Jimenez-Vergara ◽  
Rodrigo Zurita ◽  
Abigail Jones ◽  
Patricia Diaz-Rodriguez ◽  
Xin Qu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Cell Shape ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Mesenchymal Stem Cell Differentiation ◽  
The Impact

Impact of Ultrasound Therapy on Stem Cell Differentiation - A Systematic Review

2020 ◽  
Vol 15 (5) ◽  
pp. 462-472 ◽  
Author(s):  
Abdollah Amini ◽  
Sufan Chien ◽  
Mohammad Bayat
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
The Body ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Objective: This is a systematic review of the effects of low-intensity pulsed ultrasound (LIPUS) on stem cell differentiation. Background Data: Recent studies have investigated several types of stem cells from different sources in the body. These stem cells should strictly be certified and promoted for cell therapies before being used in medical applications. LIPUS has been used extensively in treatment centers and in research to promote stem cell differentiation, function, and proliferation. Materials and Methods: The databases of PubMed, Google Scholar, and Scopus were searched for abstracts and full-text scientific papers published from 1989-2019 that reported the application of LIPUS on stem cell differentiation. Related English language articles were found using the following defined keywords: low-intensity pulsed ultrasound, stem cell, differentiation. Criteria for inclusion in the review were: LIPUS with frequencies of 1–3 MHz and pulsed ultrasound intensity of <500 mW/cm2. Duration, exposure time, and cell sources were taken into consideration. Results: Fifty-two articles were selected based on the inclusion criteria. Most articles demonstrated that the application of LIPUS had positive effects on stem cell differentiation. However, some authors recommended that LIPUS combined with other physical therapy aides was more effective in stem cell differentiation. Conclusions: LIPUS significantly increases the level of stem cell differentiation in cells derived mainly from bone marrow mesenchymal stem cells. There is a need for further studies to analyze the effect of LIPUS on cells derived from other sources, particularly adipose tissue-derived mesenchymal stem cells, for treating hard diseases, such as osteoporosis and diabetic foot ulcer. Due to a lack of reporting on standard LIPUS parameters in the field, more experiments comparing the protocols for standardization of LIPUS parameters are needed to establish the best protocol, which would allow for the best results.

Induction of mesenchymal stem cell differentiation by co-culturing with mature cells in double-layered 2-methacryloyloxyethyl phosphorylcholine polymer hydrogel matrices

2021 ◽  
Author(s):  
Kazuhiko Ishihara ◽  
Miu Kaneyasu ◽  
Kyoko Fukazawa ◽  
Ren Zhang ◽  
Yuji Teramura
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Stem Cell Differentiation ◽  
Water Soluble ◽  
Polymer Hydrogel ◽  
Differentiated Cells ◽  
Mesenchymal Stem Cell Differentiation ◽  
Hydrogel Matrix ◽  
Zwitterionic Polymer ◽  
A Cell

The effects of differentiated cells on stem cell differentiation were analyzed by co-culture using a cell-encapsulated double-layered hydrogel system. As a polymer hydrogel matrix, a water-soluble zwitterionic polymer having both...

scholarly journals CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Gabriel N Aughey ◽  
Alicia Estacio Gomez ◽  
Jamie Thomson ◽  
Hang Yin ◽  
Tony D Southall
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Ectopic Expression ◽  
Stem Cell Differentiation ◽  
Cell Types ◽  
Regulatory Elements ◽  
Chromatin Accessibility ◽  
Global Changes

During development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibility changes in the development of somatic lineages are not well defined. Here we show that cell-specific chromatin accessibility data can be produced via ectopic expression of E. coli Dam methylase in vivo, without the requirement for cell-sorting (CATaDa). We have profiled chromatin accessibility in individual cell-types of Drosophila neural and midgut lineages. Functional cell-type-specific enhancers were identified, as well as novel motifs enriched at different stages of development. Finally, we show global changes in the accessibility of chromatin between stem-cells and their differentiated progeny. Our results demonstrate the dynamic nature of chromatin accessibility in somatic tissues during stem cell differentiation and provide a novel approach to understanding gene regulatory mechanisms underlying development.

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