GROWTH AND COLOUR PERFORMANCE OF THE CROSSBREED MARBLE STRAIN Betta splendens AND Betta imbellis

Betta ornamental fish of marble strain is a strain which is prefered by the hobbyists, because of its unique color, expensive price, and difficult to obtain the fish color strains. Therefore strain marble betta fish is still dominant for export quality for national and international contests. The aim of this study was to observe the growth and color performance of the crossedbreed marble strains of wild betta, Betta imbellis crossed with Betta splendens. The hybrids of B. imbellis (f) x B. splendens (m) (D) had a specific length and weight rate, (1,113 ± 0.04%/day; 2,531 ± 0.14%/day; 26.61 ± 2.02 mm) which was better than that of the hybrids B. imbellis (m) x B. splendens (f) (C) (1,099 ± 0.02%/day; 2,244 ± 0.13%/day; 25.97 ± 1.33 mm; and 0.0027 ± 0.0003 mm). However, the survival rate of the C hybrids (42.19 ± 11.42%) was higher than those of D (33.67%-17.08%). Based on the obtained results of the color identification the hybrids had as many as 15 characters of color, homozygous strains of marble which became the target of as much as 1%.

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MASCULINIZATION OF Betta splendens LARVAE WITH JAVA PEPPER (Piper retrofractum Vahl) EXTRACT THROUGH IMMERSION

e-Jurnal Rekayasa dan Teknologi Budidaya Perairan ◽

10.23960/jrtbp.v8i2.p985-990 ◽

2020 ◽

Vol 8 (2) ◽

pp. 985

Author(s):

Ajie Prayoga ◽

Ahmad Fahrul Syarif ◽

Eva Prasetiyono ◽

Suci Puspita Sari

Keyword(s):

Survival Rate ◽

Sex Ratio ◽

Positive Control ◽

Negative Control ◽

Siamese Fighting Fish ◽

Ornamental Fish ◽

Betta Splendens ◽

Male Ratio ◽

Piper Retrofractum ◽

The One

Siamese betta fighting fish (Betta splendens) is the one of exports comodity of ornamental fish in Indonesia, but the problems of culture that fish is sex ratio of female higher than male. This research aimed to evaluate the effectiveness of java long pepper (Piper retrofractum Vahl) for betta siamese fighting fish masculinizations through larval immersion. This research was designed by randomized completely design with three treatments of different java long papper doses (0,5 mg/L, 1 mg/L, and 2 mg/L), positive control (17α-metiltestosteron 20 mg/L), and negative control with there replications for each treatments. The treatments of java long pepper doses was given to thirty larvae (D4) through immersion during 24 hours. The research showed that java long pepper extract with dose 2 mg/L obtained the higher male ratio (59,82%) compared with dose 0,5 mg/L (45,11%), 1 mg/L (53,92%), and negative control (23,23%). Java long pepper extract with dose 2 mg/Lincreased the male ratio 36,59% compared with negative control. Java long pepper extract have no effect for survival rate of siamese betta fighting larvae during cultured.

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PERBEDAAN WAKTU PEMELIHARAAN TELUR DAN LARVA OLEH INDUK JANTAN TERHADAP DAYA TETAS DAN KELANGSUNGAN HIDUP LARVA IKAN CUPANG (Betta splendens)

Jurnal Akuakultur Sungai dan Danau ◽

10.33087/akuakultur.v3i2.40 ◽

2018 ◽

Vol 3 (2) ◽

pp. 76

Author(s):

M Fauzan ◽

Muhammad Sugihartono ◽

M Yusuf Arifin

Keyword(s):

Survival Rate ◽

Water Level ◽

Sea Water ◽

Fish Larvae ◽

Ornamental Fish ◽

Betta Splendens ◽

Male Parent ◽

Long Time ◽

Absolute Length ◽

Body Shapes

Ornamental fish is a type of fish that lives in both fresh and sea water which has attractive and beautiful body shapes or colors. One type of ornamental fish with its own uniqueness compared to other ornamental fish is Betta fish (B. splendens.). As an alternative, the test was carried out for a long time the male parent who cared for larvae at different times on the effect of survival of betta fish larvae on the type of plaque (B. splendens). The parent used in this study is a parent of 4 months old which is prepared as many as 1 pair / container of maintenance and in total there are 12 pairs. The research container used in this study was an aquarium with a water level of 10 cm, 12 pieces. Each container is labeled according to randomization and is equipped with aeration with a small pressure. The water used in this study is water originating from excavated wells. Before use water is first deposited to remove sedimentation. For the best treatment, the highest survival rate of betta (B. splendens) larvae was found in treatment A, which was 11.56%, followed by treatment B (8.44%), then treatment C (7.51%) and treatment D (4.81%). The highest absolute length growth is found in treatment D, which is equal to 14.33 mm, followed by treatment C (14.00 mm), then treatment B (12.83 mm) and treatment A (11.17 mm).Keywords: Plaque Betta Fish, Maintenance Length by Male Parent, Survival, Growth, Water Quality.AbstrakIkan hias merupakan jenis ikan yang hidup di air tawar maupun laut yang mempunyai bentuk atau warna tubuh menarik dan indah. Salah satu jenis ikan hias dengan keunikan tersendiri dibandingkan ikan hias lainnya adalah ikan cupang (B. splendens.). Sebagai alternatif uji yang dilakukan adalah lama induk jantan yang mengasuh larva dengan waktu yang berbeda terhadap pengaruh kelangsungan hidup larva ikan cupang jenis plakat (B. splendens).Induk yang digunakan dalam penelitian ini adalah induk yang berumur 4 bulan yang di persiapkan sebanyak 1 pasang / wadah pemeliharaan dan secara total berjumlah 12 pasang.Wadah penelitian yang digunakan dalampenelitian ini adalah akuarium dengan ketinggian air 10 cm, sebanyak 12 buah. Masing-masing wadah diberi label sesuai dengan pengacakan dan dilengkapi aerasi dengan tekanan yang kecil. Air yang digunakan dalam penelitian ini adalah air yang berasal dari sumur galian. Sebelum digunakan air terlebih dahulu diendapkan untuk menghilangkan sedimentasi. Untuk perlakuan yang terbaik menunjukkan bahwa tingkat kelangsungan hidup larva ikan cupang (B. splendens) tertinggi terdapat pada perlakuan A yaitu sebesar 11,56% selanjutnya diikuti perlakuan B (8,44%), kemudian perlakuan C (7,51%) dan perlakuan D (4,81%). Pertumbuhan panjang mutlak tertinggi terdapat pada perlakuan D yaitu sebesar 14,33 mm selanjutnya diikuti oleh perlakuan C (14,00 mm), kemudian perlakuan B (12,83 mm) dan perlakuan A (11,17 mm).Kata Kunci :Ikan cupang Plakat, Lama Waktu Pemeliharaan Oleh Induk Jantan, Kelangsungan Hidup, Pertumbuhan, Kualitas air.

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Effect Of Animal And Plant Protein Diets On Growth And Fecundity In Ornamental Fish, Betta Splendens (Regan)

Israeli Journal of Aquaculture - Bamidgeh ◽

10.46989/001c.20334 ◽

2003 ◽

Author(s):

Kunchitham Sampath ◽

Raja James

Keyword(s):

Ornamental Fish ◽

Betta Splendens ◽

Plant Protein ◽

Protein Diets

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Combination of Mycorrhizal Symbiosis and Root Grafting Effectively Controls Nematode in Replanted Coffee Soil

Plants ◽

10.3390/plants9050555 ◽

2020 ◽

Vol 9 (5) ◽

pp. 555 ◽

Cited By ~ 1

Author(s):

The Trinh Pham ◽

Bach Long Giang ◽

Ngoc Hoi Nguyen ◽

Pham Nguyen Dong Yen ◽

Vo Do Minh Hoang ◽

...

Keyword(s):

Survival Rate ◽

Coffea Canephora ◽

Mycorrhizal Symbiosis ◽

Poor Survival ◽

Soil Pathogens ◽

Coffee Plants ◽

Survival And Growth ◽

Combined Technique ◽

Growth Indicators ◽

Better Than

Replanting for sustainable development is one of the critical missions of the coffee industry in the Daklak province, Vietnam. However, this plan has been faced with many difficulties including poor survival and growth rates due to the low nematode tolerance of young coffee plants in replanted fields. Mycorrhizal symbiosis and grafting have been applied separately but not yet resulted in the expected results of the replanting plan. Whether the combination of them would help managing nematode in the soil and consequently enhance the replanted efficiency is largely unknown. Mycorrhizal symbiosis was applied to Coffea canephora plants or/and grafted onto Coffea liberica rootstock, which were grown and compared to the untreated ones in both net-house-pots and the replanted plantation. The survival rate, growth indicators and the soil pathogens were monitored during the experimental periods. The combination of grafting and mycorrhiza symbiotic techniques significantly decreased the nematode densities in the replanted soil. As a result, the survival rate and growth indicators of the coffee in the replanted soil treated by the combined technique were better than treated by the two separate techniques. The results suggested that the combination of grafting and mycorrhiza symbiotic techniques would propose a potentially effective Pratylenchus coffeae and Meloidogyne incognita nematode management in replanted coffee fields in the Daklak province, Vietnam.

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Daya hidup planlet karet asal in vitro microcutting pada berbagai periode penutupan sungkup plastik dan komposisi media tumbuh Survival rate of in vitro microcutting-derived rubber plantlets on various plastic cover closed periods and medium compositions

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v80i1.46 ◽

2016 ◽

Vol 80 (1) ◽

Author(s):

. SUMARYONO ◽

Masna Maya SINTA ◽

. NURHAIMI-HARIS

Keyword(s):

Survival Rate ◽

Hevea Brasiliensis ◽

Tree Canopy ◽

Ex Vitro ◽

Media Composition ◽

Transparent Plastic ◽

Growing Medium ◽

Major Bottleneck ◽

Better Than

AbstractIn vitro culture through microcutting technology can be used for clonal propagation of rubber (Hevea brasiliensis Muell. Arg.) rootstocks. Acclimatization of in vitro plantlets to ex vitro conditions is a major bottleneck in the micropropagation of many plants.This research was conducted to study the effect of plastic cover closed period and media composition on the survival rate of rubber plantlets. Plantlets derived from microcutting were planted on plastic pots containing a mixture of soil, cocopeat, dung manure, and sand or zeolite. The plantlets were then placed inside a closed transparent plastic cover that opened after 2, 3, 4 and 6 weeks. The cover was placed under tree canopy. The second experiment used the same media composition with or without cocopeat and with sand or zeolite. At 1.5 month after culture, observation was done on the number of survived plantlets, plantlet height and the percentage of rooted plantlets. The results show that the best coverclosed period was six weeks and the best growing medium was a mixture of soil, cocopeat, dung manure, and zeolite (6:2:1:1v/v). On the two combined treatments, the survival rate was 73.3% after 1.5 month of acclimatization. The use of zeolite and a higher soil percentage gave positive influences on rubber plantlet survival rate. The second experiment results confirmed that the use of zeolite was better than sand and the use of cocopeat was definitely needed. It can be concluded that the best of acclimatization of rubber plantlets from microcutting was on a medium mixture of soil, cocopeat, dung manure, and zeolite (6:2:1:1) and placed inside a closed plastic cover for six weeks before the cover was opened gradually. AbstrakKultur in vitro melalui teknologi microcutting dapat digunakan untuk perbanyakan klonal batang bawah tanaman karet (Hevea brasiliensis Muell. Arg.). Aklimatisasi planlet in vitro ke kondisi ex vitro merupakan hambatan utama pada mikropropagasi berbagai jenis tanaman. Penelitian ini dilakukan untuk mempelajari pengaruh lama penutupan sungkup plastik dan komposisi media tumbuh terhadap daya hidup planlet karet. Planlet karet asal microcutting ditanam pada pot plastik berisi media dengan berbagai campuran tanah, cocopeat, pupuk kandang, dan pasir atau zeolit. Planlet selanjutnya diletakkan di dalam sungkup plastik transparan tertutup rapat yang dibuka setelah 2, 3, 4 dan 6 minggu. Sungkup plastik diletakkan di bawah tajuk pepohonan. Percobaan kedua menggunakan komposisi media serupa dengan atau tanpa cocopeat dan dengan pasir atau zeolit. Pada umur 1,5 bulan, pengamatan dilakukan terhadap jumlah planlet yang hidup, tinggi planlet, dan persentase planlet yang berakar. Hasil penelitian menunjukkan bahwa lama penyungkupan terbaik adalah enam minggu dan media tumbuh terbaik adalah campuran tanah, cocopeat, pupuk kandang, dan zeolit (6:2:1:1 v/v). Pada kombinasi kedua perlakuan tersebut, daya hidup planlet karet mencapai 73,3% setelah 1,5 bulan aklimatisasi. Penggunaan zeolit dan persentase tanah yang lebih tinggi berpengaruh positif terhadap daya hidup planlet karet. Hasil percobaan kedua menegaskan bahwa penggunaan zeolit lebih baik daripada pasir dan penggunaan cocopeat mutlak diperlukan. Dapat disimpulkan bahwa aklimatisasi planlet karet asal microcutting terbaik dilakukan pada media campuran tanah, cocopeat, pupuk kandang, zeolit (6:2:1:1) dan diletakkan di dalam sungkup plastik tertutup selama enam minggu sebelum sungkup dibuka secara bertahap.

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Survival Rate and Growth of Fighting Fish Larvae (Betta splendens Regan) Fed on Various Live Foods

Jurnal Akuakultur Indonesia ◽

10.19027/jai.4.13-16 ◽

2007 ◽

Vol 4 (1) ◽

pp. 13 ◽

Cited By ~ 1

Author(s):

T. Budiardi ◽

T. Nursyams ◽

Agus Oman Sudrajat

Keyword(s):

Survival Rate ◽

Fish Larvae ◽

Betta Splendens ◽

The Other ◽

Live Food ◽

Growth And Survival ◽

Total Length ◽

Other Hand

Larval of fighting fish (Betta splendens Regan) requires precise live foods for its growth and survival. In this experiment, fish larvae were fed on either Paramecium + Artemia, Paramecium + Artemia + Tubifex, Paramecium + Moina, or Paramecium + Moina + Tubifex. The fish were fed Paramecium from day-2 till day-7 after hatching. There after, the live food was changed according to the treatments till day-28. Results showed that fish fed on Paramecium + Artemia significantly had the highest total length (12.63 mm) than other treatments (11.86 mm). On the other hand, survival rate of fish had no significant affected by the treatments. Keywords: fighting fish, Betta splendens, Paramecium, Moina, Artemia, Tubifex, larvae ABSTRAK Larva ikan betta (Betta splendens Regan) membutuhkan jenis pakan alami yang tepat bagi kelangsungan hidup dan pertumbuhannya. Pada penelitian ini, larva ikan diberi pakan berupa Paramecium + Artemia, Paramecium + Artemia + Tubifex, Paramecium + Moina, atau Paramecium + Moina + Tubifex. Ikan diberi pakan pakan berupa Paramecium dari hari ke-2 hingga hari ke-7. Setelah itu, pemberian pakan alami diubah berdasarkan masing-masing perlakuan hingga hari ke-28. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan Paramecium + Artemia memiliki tubuh secara signifikan lebih panjang (12,63 mm) dibandingkan perlakuan lainnya (11,86 mm). Sementara itu, kelangsungan hidup tidak dipengatuhi oleh perlakuan. Kata kunci: ikan betta, Betta splendens, Paramecium, Moina, Artemia, Tubifex, larva

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HLA-Mismatched GPBSCs infusion therapy in refractory Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis: A observational study from a single center

10.21203/rs.2.22776/v3 ◽

2020 ◽

Author(s):

Yue Song ◽

Jingshi Wang ◽

Yini Wang ◽

Zhao Wang

Keyword(s):

Survival Rate ◽

Hemophagocytic Lymphohistiocytosis ◽

Median Survival Time ◽

Epstein Barr Virus ◽

Control Group ◽

Short Term ◽

Barr Virus ◽

Ebv Dna ◽

Epstein Barr ◽

Better Than

Abstract Background: Hemophagocytic lymphohistiocytosis (HLH) is a severe or even fatal inflammatory state. Epstein–Barr virus (EBV) infection associated HLH (EBV-HLH) is one of the most common secondary HLH and suffers a very poor prognosis. Allo-HSCT is often required for refractory EBV-HLH, but some patients still cannot proceed to the next allo-HSCT due to various factors. This study aimed to observe the efficacy of HLA-mismatched granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood stem cells (GPBSCs) infusion for refractory EBV-HLH. Methods: A retrospective case-control study of refractory EBV-HLH patients with GPBSCs infusion from HLA-mismatched donors after chemotherapy (as GPBSCs group) and sole chemotherapy (as control group) was performed. Efficacy was evaluated 2 and 4 weeks and all patients were followed up until 1 March 2018.Results: There were 18 cases who accepted infusion between March 2016 and Sep 2017 and 19 were randomly selected from refractory EBV-HLH patients who underwent salvage therapy during the same period for the control group. In GPBSCs group, WBC (p=0.017), Fbg (p=0.040), ferritin (p=0.039) improved significantly after treatment. The overall response rate was 66.7% (CR 22.2%, PR 44.4%). However, there is no significant differences in changes of WBC, HGB, PLT, TG, Fbg, Ferritin, AST, ALT, T-bil between two groups. Only the Fbg level was recovered better in the GPBSCs infusion group (p=0.003). In the GPBSCs group, EBV-DNA decreased significantly after 2 weeks (p=0.001) and 4 weeks (p=0.012) after treatment, and the effect of the decrease was significantly better than that of the chemotherapy alone group in 2 weeks but not 4 weeks (p2w=0.011, p4w=0.145). The median survival time in the infusion group was 20.4 weeks [95%CI 10.9, 29.9], and the median survival time in the control group was 10.8 weeks [95%CI 0-24.34]. In the short-term, the infusion group’s survival rate was better (2-month 88.89% vs. 52.63%, p=0.008; 3-month 83.33% vs. 47.09%, p=0.012), but there was no difference in OS (p=0.287). Conclusions: Infusing GPBSCs combined with chemotherapy is effective, especially in decreasing EBV-DNA, performs better than chemotherapy alone, and improve short term survival rate. GPBSCs infusion is suggested as a bridging treatment method to allo-HSCT.

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HLA-Mismatched GPBSCs infusion therapy in refractory Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis: A observational study from a single center

10.21203/rs.2.22776/v2 ◽

2020 ◽

Author(s):

Yue Song ◽

Jingshi Wang ◽

Yini Wang ◽

Zhao Wang

Keyword(s):

Survival Rate ◽

Hemophagocytic Lymphohistiocytosis ◽

Median Survival Time ◽

Epstein Barr Virus ◽

Control Group ◽

Short Term ◽

Barr Virus ◽

Ebv Dna ◽

Epstein Barr ◽

Better Than

Abstract Background: Hemophagocytic lymphohistiocytosis (HLH) is a severe or even fatal inflammatory state. Epstein–Barr virus (EBV) infection associated HLH (EBV-HLH) is one of the most common secondary HLH and suffers a very poor prognosis. Allo-HSCT is often required for refractory EBV-HLH, but some patients still cannot proceed to the next allo-HSCT due to various factors. This study aimed to observe the efficacy of HLA-mismatched granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood stem cells (GPBSCs) infusion for refractory EBV-HLH. Methods: A retrospective case-control study of refractory EBV-HLH patients with GPBSCs infusion from HLA-mismatched donors after chemotherapy (as GPBSCs group) and sole chemotherapy (as control group) was performed. Efficacy was evaluated 2 and 4 weeks and all patients were followed up until 1 March 2018. Results: There were 18 cases who accepted infusion between March 2016 and Sep 2017 and 19 were randomly selected from refractory EBV-HLH patients who underwent salvage therapy during the same period for the control group. In GPBSCs group, WBC (p=0.017), Fbg (p=0.040), ferritin (p=0.039) improved significantly after treatment. The overall response rate was 66.7% (CR 22.2%, PR 44.4%). However, there is no significant differences in changes of WBC, HGB, PLT, TG, Fbg, Ferritin, AST, ALT, T-bil between two groups. Only the Fbg level was recovered better in the GPBSCs infusion group (p=0.003). In the GPBSCs group, EBV-DNA decreased significantly after 2 weeks (p=0.001) and 4 weeks (p=0.012) after treatment, and the effect of the decrease was significantly better than that of the chemotherapy alone group in 2 weeks but not 4 weeks (p2w=0.011, p4w=0.145). The median survival time in the infusion group was 20.4 weeks [95%CI 10.9, 29.9], and the median survival time in the control group was 10.8 weeks [95%CI 0-24.34]. In the short-term, the infusion group’s survival rate was better (2-month 88.89% vs. 52.63%, p=0.008; 3-month 83.33% vs. 47.09%, p=0.012), but there was no difference in OS (p=0.287). Conclusions: Infusing GPBSCs combined with chemotherapy is effective, especially in decreasing EBV-DNA, performs better than chemotherapy alone, and improve short term survival rate. GPBSCs infusion is suggested as a bridging treatment method to allo-HSCT.

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TREATMENT OF VIBRIOSIS DISEASE (Vibrio harveyi) IN VANAME SHRIMP (Litopenaeus vannamei, Boone 1931) USING Avicennia alba LEAVES EXTRACT

e-Jurnal Rekayasa dan Teknologi Budidaya Perairan ◽

10.23960/jrtbp.v8i1.p909-916 ◽

2019 ◽

Vol 7 (2) ◽

pp. 909

Author(s):

Dian Rusadi ◽

Wardiyanto Wardiyanto ◽

Rara Diantari

Keyword(s):

Survival Rate ◽

Clinical Symptoms ◽

Vibrio Harveyi ◽

Time Of Death ◽

Mangrove Plant ◽

Vibrio Infection ◽

The Mean ◽

Avicennia Alba ◽

Mean Time ◽

Better Than

Avicennia alba is a mangrove plant that often ued by coastal society as a traditional medecine, it is potential to be dveloped as the sources of pharmaceutical. The aim of the research is to examine the effect of Avicennia alba leaves extract various concentrations to Vibrio harveyi infection on vaname shrimp. The addition of the extract is thought to be used as an antibacterial so that it can give effect to the attack of V. harveyi in vaname shrimp. Vaname shrimps (total of 120 shrimps) with a weight of 10±2g/ind were injected intramuscularly with V. harveyi bacteria in 107 CFU/ml density. After the occurrences of clinical symptoms, the shrimps were immersed into A. alba leaves extract with concentration 0 ppm, 150 ppm, 250 ppm, and 350 ppm for 21 days. The results showed that the addition leaves extract can increase shrimp’s survival rate as much as 46.67% and increase the shrimp’s ability to prevent (RPS) V. harveyi infection untill of 70±15%. The mean time of death (MTD) was 106±18.33 hours, it caused there ar active compounds contained such as saponin, tannin, and steroid be suspected antibacterial so that they can protect shrimp from the effect of Vibrio infection damage. Giving a concentration of 250 ppm Avicennia alba leaves extract can treat vaname shrimp which was attacked by V. harveyi better than other treatments.

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