scholarly journals MASCULINIZATION OF Betta splendens LARVAE WITH JAVA PEPPER (Piper retrofractum Vahl) EXTRACT THROUGH IMMERSION

2020 ◽  
Vol 8 (2) ◽  
pp. 985
Author(s):  
Ajie Prayoga ◽  
Ahmad Fahrul Syarif ◽  
Eva Prasetiyono ◽  
Suci Puspita Sari
Keyword(s):  
Survival Rate ◽  
Sex Ratio ◽  
Positive Control ◽  
Negative Control ◽  
Siamese Fighting Fish ◽  
Ornamental Fish ◽  
Betta Splendens ◽  
Male Ratio ◽  
Piper Retrofractum ◽  
The One

Siamese betta fighting fish (Betta splendens) is the one of exports comodity of ornamental fish in Indonesia, but the problems of culture that fish is sex ratio of female higher than male. This research aimed to evaluate the effectiveness of java long pepper (Piper retrofractum Vahl) for betta siamese fighting fish masculinizations through larval immersion. This research was designed by randomized completely design with three treatments of different java long papper doses (0,5 mg/L, 1 mg/L, and 2 mg/L), positive control (17α-metiltestosteron 20 mg/L), and negative control with there replications for each treatments. The treatments of java long pepper doses was given to thirty larvae (D4) through immersion during 24 hours. The research showed that java long pepper extract with dose 2 mg/L obtained the higher male ratio (59,82%) compared with dose 0,5 mg/L (45,11%), 1 mg/L (53,92%), and negative control (23,23%). Java long pepper extract with dose 2 mg/Lincreased the male ratio 36,59% compared with negative control. Java long pepper extract have no effect for survival rate of siamese betta fighting larvae during cultured.

scholarly journals UJI DAYA ANTIINFLAMASI EKSTRAK ETANOLIK KULIT TERONG BELANDA (Solanum betaceum, Cav.) PADA MENCIT JANTAN YANG DIINDUKSI KARAGENIN

2020 ◽  
Vol 16 (02) ◽  
pp. 86
Author(s):  
Margareta Retno Priamsari ◽  
Rina Ayu Krismonikawati
Keyword(s):  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Test Material ◽  
Edema Formation ◽  
Flavonoid Compounds ◽  
The One ◽  
Post Hoc ◽  
Solanum Betaceum

ABSTRACT Inflammation is a normal response to injury due to release of chemical mediators such as prostaglandins. Solanum betaceum Cav. skin contains flavonoid compounds. This study aims to determine the antiinflammatory power and the effect of variations in the dose of ethanolic extract of the skin of Solanum betaceum Cav on Swiss male strain mice caragenin-induced. The ethanolic extract of Solanum betaceum Cav skin was obtained using remaseration method with 96% ethanol solvent. Antiinflammatory power test using edema formation method with induction of caragenin 1% intraplantar 30 minutes before administration of the test material. Tests were carried out on 25 mice records which were divided into 5 groups namely negative control (0.5% CMC Na suspension), positive control (diclofenac sodium suspension in 0.5% CMC Na dose 7 mg/kgBW) and given suspension respectively. Dutch eggplant skin ethanolic extract in CMC Na 0.5% dose 70 mg/kgBW; 140 mg/kgBW; 280 mg/kgBW orally. The parameters observed were edema thickness measured using calipers at 30 minute intervals for 360 minutes. The results of edema thickness difference are used to calculate the Area Under Curve (AUC) and the percentage of antiinflammatory power (% AIP). Dat a were analyzed statistically with the One Way Anova test and continued by the Post Hoc Test with a 95% confidence level. The results showed that the ethanolic extract of Solanum betaceum Cav skin contains flavonoid compounds and has antiinflammatory power. The greater the dose of ethanolic extract of Solanum betaceum Cav skin, the greater the anti-inflammatory power. Keywords: Solanum betaceum Cav., antinflammation, caragenin

THE EFFECTIVENESS TEST OF MATOA (PometiaPinnata) ENDOPHYTIC BACTERIA TOWARDS BACTERIA NOSOCOMIAL INFECTION

2020 ◽  
Vol 1 (1) ◽  
pp. 37-40
Author(s):  
Denny Chandra Halid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infection ◽  
Staphylococcus Epidermidis ◽  
Endophytic Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Bacterial Isolates ◽  
Series Design ◽  
Inhibition Zones ◽  
The One

This study aims to determine the effectiveness of Matoa (Pometiapin-nata) endophytic bacteria towards bacteria nosocomial infection namely Pseudomonas aeruginosa and Staphylococcus epidermidis. The subjects in the study were Matoa plant endophytic bacterial isolates on the stem (tw-igs). The positive control used is meropenem & negative control of aquades. This type of research uses quasi-experiments with a research design us-ing the One-Group Time-Series Design. The result of the study shows that there are 2 endophytic bacterial iso-lates in Matoa plants namely BEM 1 and BEM 2. Both endophytic bacterial isolates can kill and inhibits bacterial nosocomial infections Pseudomonas aeruginosa and Staphylococcus epider-midis with inhibition zones in the range of 16mm-22mm with a strong category very strong that it has the po-tential to be used as an antibacterial

scholarly journals Effect of letrozole on masculinization of Siamese fighting fish (Betta splendens)

2015 ◽  
Vol 7 (1) ◽  
pp. 425-433
Author(s):  
Milind B. Katare ◽  
N. Basavaraja ◽  
Harshavardhan D. Joshi ◽  
Chirom Archana
Keyword(s):  
Sex Ratio ◽  
Oral Administration ◽  
Ovarian Development ◽  
Siamese Fighting Fish ◽  
Betta Splendens ◽  
Testicular Development ◽  
Progeny Testing ◽  
Male Population ◽  
Dorsal Fin ◽  
Different Doses

The aim of this study was to evaluate the effect of letrozole (a non-steroidal aromatase inhibitor) on masculinization of Siamese fighting fish (Betta splendens). Different doses of letrozole 50, 100, 150 and 200 ppm (mg/kg feed) were incorporated into diet and fed for periods of 30, 40 or 50 days. Immersion treatment of letrozole at selected doses (250, 500, 750 and 1,000μg/l) for 3 h each on third, fifth and eighth day-post-hatching (dph) (Trial 1) and fourth, sixth and eighth dph (Trial 2) was given.The oral administration of letrozole for 30, 40 or 50 days did not have any significant effect on the sex ratio of B. splendens. The immersion treatment of letrozole induced 100% masculinization at 500 and 1000μg/l in trial 1, while it yielded only 66.66 and 90% male population at 500 and 1000 μg/l in Trial 2. The dietary administration of letrozole for 30 and 50 days caused tail deformities and/or rudimentary and absence of dorsal fin. The progeny testing of males from letrozole treated (both oral and immersion) groups indicate that the sex ratio of progenies of each of the males tested did not differ significantly from that of control, indicating that all those males carried XY genotype. Letrozole treatments suppressed ovarian development (atretic oocytes were common). However, the testicular development was unaffected. The study revealed that immersion treatment of letrozole was more effective in inducing masculinization of B. splendens than the oral administration of letrozole.

scholarly journals APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei))

2016 ◽  
Vol 10 (2) ◽  
pp. 121-127
Author(s):  
Widanarni Widanarni ◽  
Sukenda Sukenda ◽  
Ghita Ryan Septiani
Keyword(s):  
Survival Rate ◽  
Immune Responses ◽  
Litopenaeus Vannamei ◽  
Ipomoea Batatas ◽  
Challenge Test ◽  
Positive Control ◽  
Negative Control ◽  
White Shrimp ◽  
Infectious Myonecrosis Virus ◽  
Synbiotic Supplementation

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.493±0.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.93±0.025-6.97±0.019% and had higher values than controls (A and B) (P<0.05). Meanwhile, feed conversion value in C and D (1.54±0.142 and 1.58±0.117) were lower than controls (P<0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P<0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals PEMANFAATAN EKSTRAK BUNGA KECOMBRANG (Etlingera elatior jack R.M.sm) SEBAGAI LARVASIDA NYAMUK (Culex quinquifasciatus Say)

2020 ◽  
Vol 10 ◽  
pp. 44-49
Author(s):  
Indra Rahmawati ◽  
Bagus Uda Palgunadi ◽  
Lailia Dwi Kusuma Wardhani ◽  
Ni Made Dewi Lestari
Keyword(s):  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mosquito Larvae ◽  
Test Results ◽  
Experimental Animals ◽  
Flower Extract ◽  
The One ◽  
One Way Anova ◽  
Etlingera Elatior

This study aims to determine the effectiveness of kecombrang flower extract (Etlingera elatior jack R.M.sm) on the death of Culex quenquefasciatus Say larvae. Experimental animals used were Culex quenquefasciatus Say larvae, amounting to 625 tails. The design used was a randomized complete design with 5 treatments and 5 replications. The five treatments consisted of two test groups, namely the treatment group consisting of concentrations of 5%, 10%, 15%, and the control group consisting of positive control namely abate and negative control namely aquades. Observation of mosquito larvae death was done every 2 hours for 12 hours. The data obtained were analyzed using the one-way ANOVA test and continued with the Duncan test. Based on the results of the one way ANOVA calculation the value of sig.0.00 <0.05 means that there are significant differences between treatments. Duncan test results on the treatment of 10% and 15% kecombrang flower extract have the ability as a larvicide high and equal to positive control (abate). Based on the results of the study it was concluded that the extract of kecombrang flower (Etlingera elatior jack R.M.sm) had the ability to larvae against the Culex quenquefasciatus Say mosquito.

scholarly journals Detection of Mycobacterium marinum in clinically asymptomatic Siamese fighting fish (Betta splendens) from ornamental fish shops in Chiang Mai Province, Thailand

2017 ◽  
Vol 7 (6) ◽  
pp. 344-346 ◽  
Author(s):  
Anucha Sirimalaisuwan ◽  
◽  
Pradthana Teeraruk ◽  
Penpisoot Kanjanapitakchai ◽  
Thattawan Kaewsakhorn ◽  
...  
Keyword(s):  
Siamese Fighting Fish ◽  
Ornamental Fish ◽  
Mycobacterium Marinum ◽  
Betta Splendens ◽  
Chiang Mai ◽  
Chiang Mai Province

scholarly journals GROWTH AND COLOUR PERFORMANCE OF THE CROSSBREED MARBLE STRAIN Betta splendens AND Betta imbellis

2015 ◽  
Vol 10 (2) ◽  
pp. 101 ◽  
Author(s):  
Eni Kusrini ◽  
Riani Rahmawati ◽  
Siti Murniasih ◽  
Ruby Vidia Kusumah ◽  
Anjang Bangun Prasetio
Keyword(s):  
Survival Rate ◽  
Ornamental Fish ◽  
Betta Splendens ◽  
Unique Color ◽  
Color Identification ◽  
Color Strains ◽  
Export Quality ◽  
Specific Length ◽  
Better Than

Betta ornamental fish of marble strain is a strain which is prefered by the hobbyists, because of its unique color, expensive price, and difficult to obtain the fish color strains. Therefore strain marble betta fish is still dominant for export quality for national and international contests. The aim of this study was to observe the growth and color performance of the crossedbreed marble strains of wild betta, Betta imbellis crossed with Betta splendens. The hybrids of B. imbellis (f) x B. splendens (m) (D) had a specific length and weight rate, (1,113 ± 0.04%/day; 2,531 ± 0.14%/day; 26.61 ± 2.02 mm) which was better than that of the hybrids B. imbellis (m) x B. splendens (f) (C) (1,099 ± 0.02%/day; 2,244 ± 0.13%/day; 25.97 ± 1.33 mm; and 0.0027 ± 0.0003 mm). However, the survival rate of the C hybrids (42.19 ± 11.42%) was higher than those of D (33.67%-17.08%). Based on the obtained results of the color identification the hybrids had as many as 15 characters of color, homozygous strains of marble which became the target of as much as 1%.

scholarly journals ANTIBACTERIAL ACTIVITY OF MIANA LEAF ESTRACT (Coleus atropurpereus) ON Steptococcus aureus

2020 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Anita Anita ◽  
Mujahidah Basarang ◽  
Rahmawati Rahmawati
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Laboratory Observation ◽  
Extract Concentration ◽  
Observation Method ◽  
The One ◽  
Growth Of Bacteria

                                               ABSTRACTMiana leaf(Coleus atrropurpureus) is the one of biofarmacology plant which using for antibacterial activity.Miana leaf contains flavonoid, tanin, saponin  which can inhibit growth of bacteria. The aims this research were study antibacterial activity of miana leaf extract on Streptococcus aureus.This research used laboratory observation method which used miana leaf extract concentration 250 mg/ml, 125 mg/ml,  62.5 mg/ml, 31,25 mg/ml, and 15,625 mg/ml. This research used tetracylin for positive control and aquadest for negative control which growth 0n 37o C  used difusion agar method. The results showed antibactrila activity  miana leaf  extract with a concentration 250 mg/ml with inhibition zone 0.00 mm, 125 mg/ml with inhibition zone 0,00 mm, 62,5 mg/ml with inhibition zone 0,00 mm, 31,25 mg/ml with inhibition zone 0,00 mm, and 15,62 mg/ml with inhibition zone 0,00 mm. For positive control showed inhibition zone 27,6 mm and negative control with inhibition zone 0,00 mm. Miana leaf extract concentration 250 mg/ml, 125 mg/ml, 62,5 mg/ml, 31,25 mg/ml and 15,62 mg/ml were not effectively inhibit Streptococcus sp.

scholarly journals In Vitro Test on the Effectiveness of Citrus limon and Allium sativum as Pediculicides

2020 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Elva Susanty ◽  
Suri Dwi Lesmana ◽  
Dedi Afandi ◽  
Ragil Yulianto ◽  
Kevin Rovi Andhika
Keyword(s):  
Allium Sativum ◽  
Positive Control ◽  
Negative Control ◽  
Citrus Limon ◽  
In Vitro Test ◽  
Anova Test ◽  
Pediculus Humanus ◽  
The One ◽  
One Way Anova

<p>Excessive usage of synthetic pediculicides, such as permethrin, lindane, and malathion, can induce resistance, environmental pollution, health problems, and even death. Efforts to prevent the side-effects of synthetic pediculicides are needed, one of which is by shifting to powerful yet safe natural pediculicides, such as Citrus limon (lemon) and Allium sativum (garlic). This study aimed to observe the effective concentration of Citrus limon and Allium sativum in vitro on the mortality of Pediculus humanus capitis (P. h. capitis). This research was an experimental laboratory study conducted in July to September 2019 in the Laboratory of Parasitology at Faculty of Medicine, University of Riau, while the making of Allium sativum extract was done at the Faculty of Mathematics and Science, University of Riau. A total of 288 samples of adult P. h. capitis was taken from children in two orphanages in Pekanbaru. Citrus limon juice and Allium sativum extract of 25%, 50%, 75%, and 100% concentrations, permethrin 1% as the positive control, and distilled water as the negative control were used in this study, and repetition was done three times. P. h. capitis mortality was observed every 10 - 120 minutes, characterized by the absence of movement of the legs and antenna. Data analysis was performed after 120 minutes with the one-way ANOVA test, LSD (=0.05), and Lethal Concentration 50 test (LC50). The one-way ANOVA test results of this study showed that statistically Citrus limon juice and Allium sativum extracts showed differences in each treatment formulation of juice on P. h. capitis mortality (Sig 0.017&lt;0.05 and Sig 0.000&lt;0.05) and LC50 juice of Citrus limon and Allium sativum extract by 51.999% and 72.426%. In this study, Citrus limon juice and Allium sativum extract had effect as pediculicides.</p>

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