scholarly journals Determination of composition of fatty acids in Saponaria officinalis L.

2021 ◽  
pp. 25-30 ◽  
Author(s):  
Liliia Budniak ◽  
Liudmyla Slobodianiuk ◽  
Svitlana Marchyshyn ◽  
Liliya Kostyshyn ◽  
Oleksandrа Horoshko
Keyword(s):  
Fatty Acids ◽  
Skin Diseases ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Qualitative Composition ◽  
Fatty Acids Composition ◽  
Quantitative Content ◽  
Main Components ◽  
Saponaria Officinalis

Treatment using medicinal plants with a long history of use is of interest to our society. These plants include Saponaria officinalis L., as well commonly known as common soapwort belongs to the family Caryophyllaceae. The herb and roots of this plant used as a blood purifier, an expectorant in bronchitis, diaphoretic and diuretic, for skin diseases, to increase bile flow. The plant contains various secondary metabolites, but there is no information on the fatty acids composition of Saponaria officinalis L. herb and roots. The aim. The aim of the present study was to determine the qualitative composition and quantitative content of fatty acids by gas chromatography/mass spectrometry method (GC/MS) in Saponaria officinalis L. herb and roots. Materials and methods. The determination of fatty acids composition of Saponaria officinalis L. herb and roots were carried out by gas chromatograph Agilent 6890N (Agilent Technologies, USA). Results. The research of Saponaria officinalis L. herb showed a mixture of unsaturated (1.9 mg/g) and saturated (1.27 mg/g) fatty acids. The main components of this raw material were linolenic (1.15 mg/g), linoleic (0.75 mg/g) and heneicosylic (0.38 mg/g) acids. The main components of this raw material were palmitic (0.38 mg/g), linoleic (0.16 mg/g) and linolenic (0.09 mg/g) acids. Conclusions. As a result of Saponaria officinalis L. study, the presence of fatty acids is established in herb and roots. Using the GC/MS method determined the qualitative composition and quantitative content of fatty acids in study raw material. Twelve fatty acids were determined in the herb of Saponaria officinalis L. The dominant fatty acids in the studied raw material were linolenic and linoleic acids, their content was 1.15 mg/g and 0.75 mg/g, respectively. Nine fatty acids were determined in the Saponaria officinalis L. roots. The palmitic acid prevailed among fatty acids, it is content was 0.38 mg/g. Our findings suggest that Saponaria officinalis L. is a promising plant because of the important role of fatty acids in different biological processes

scholarly journals Analysis of carbohydrates in Saponaria officinalis L. using GC/MS method

Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 339-345
Author(s):  
Liudmyla Slobodianiuk ◽  
Liliia Budniak ◽  
Svitlana Marchyshyn ◽  
Liliya Kostyshyn ◽  
Oleksandr Zakharchuk
Keyword(s):  
Fatty Acids ◽  
North Africa ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
Lack Of Information ◽  
The World ◽  
The Common ◽  
Free Carbohydrates ◽  
Saponaria Officinalis ◽  
Quillaic Acid

Saponaria officinalis L. (common soapwort), usually named fuller’s herb, is encountered in most of Europe, in Spain, France, Italy, for example, and also in Syria and North Africa. Saponaria officinalis L. is known in most of the world as an introduced species, often a weed, and sometimes as cultivated decorative plant. Saponaria officinalis contains a large amount of saponins, which foam during extraction with water. In addition to saponins, common soapwort also contains flavonoids, quillaic acid, fatty acids and different phenolic compounds. There is a lack of information about carbohydrates content of common soapwort. Thus the aim of this study was to determine the content of carbohydrates Saponaria officinalis L. herb and roots. The qualitative composition and quantitative content of carbohydrates in herb and roots of Saponaria officinalis L. were determined by using GC/MS method. The studies have shown that Saponaria officinalis L. herb is mainly composed of free carbohydrates such as D-glucose (3.65 mg/g), D-galactose (0.29 mg/g), D-fructose (0.20 mg/g) and D-saccharose (3.72 mg/g). In common soapwort herb, after acidic hydrolysis and derivatization with acetylated aldononitriles, D-arabinose, D-fucose, D-mannose, D-glucose, D-galactose, D-fructose and Myo-inositol were identified. Free carbohydrates in the roots of Saponaria officinalis L., including D-glucose, D-galactose and D-saccharose, were determined with GC/MS method too. D-saccharose was common among free carbohydrates of Saponaria officinalis L. in the largest amounts. Its content in herb and roots of the common soapwort was 3.72 mg/g and 25.39 mg/g respectively.

THE STUDY OF QUALITATIVE COMPOSITION AND DETERMINATION OF THE QUANTITATIVE CONTENT OF FLAVONOIDS IN RAW MATERIAL OF HELIOPHYSIS HELIANTHOIDES

Fitoterapia ◽  
2019 ◽  
Vol 2 (2) ◽  
pp. 41-44
Author(s):  
M. Yu. Pavlenko-Badnaoui ◽  
◽  
V. V. Protska ◽  
I. O. Zhuravel ◽  
I. G. Gurieva ◽  
...  
Keyword(s):  
Raw Material ◽  
Qualitative Composition ◽  
Quantitative Content

scholarly journals DETERMINATION OF CARBOHYDRATES CONTENT IN GENTIANA CRUCIATA L. BY GC/MS METHOD

2021 ◽  
pp. 124-128
Author(s):  
LILIIA BUDNIAK ◽  
LIUDMYLA SLOBODIANIUK ◽  
SVITLANA MARCHYSHYN ◽  
PAVLINA KLEPACH ◽  
YANA HONCHARUK
Keyword(s):  
Plant Material ◽  
Raw Material ◽  
Qualitative Composition ◽  
Mass Spectral Library ◽  
Spectral Library ◽  
Mass Spectral ◽  
Quantitative Content ◽  
Free Carbohydrates ◽  
Gentiana Cruciata

Objective: Thus, the aim of our research was to determine the qualitative composition and quantitative content of carbohydrates in the studied plant material with the prospect of its application as a medicinal plant raw material. Methods: The carbohydrates of the herb of Gentiana cruciata L. determined by GC/MS method. Identification of monosaccharides was based on comparing their retention times with retention times of standards of the mass spectral library NIST 02. Quantification was done by using sorbitol added to the sample. Results: The quantitative content of 4 free carbohydrates such as D-saccharose (38.39 mg/g), D-Pinitol (12.01 mg/g), D-glucose (10.05 mg/g) and D-fructose (1.69 mg/g) was established in the herb of Gentiana cruciata L. Also, this method established the qualitative composition and quantitative content of eight carbohydrates (monosaccharides and their derivatives after hydrolysis): D-glucose (29.66 mg/g), D-Pinitol (22.24 mg/g), L-arabinose (4.26 mg/g), D-galactose (3.55 mg/g), D-xylose (1.80 mg/g), L-rhamnose (1.49 mg/g), D-Dulcitol (0.76 mg/g) and D-mannose (0.44 mg/g). Conclusion: The results of the study showed that carbohydrates from the Gentiana cruciata L. can be used as important resources of new ingredients for the pharmaceutical industry.

scholarly journals Research of fatty and organic acids in Rhododendron luteum Sweet leaves

2021 ◽  
pp. 63-70
Author(s):  
Т. М. Гонтова ◽  
В. П. Гапоненко ◽  
В. В. Машталер ◽  
О. С. Мала ◽  
М. А. Кулагіна
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Organic Acids ◽  
Saturated Fatty Acids ◽  
Mass Spectrometric ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Quantitative Content ◽  
Mass Spectrometric Detector ◽  
Derivatives Of

Rhododendron luteum Sweet of the Heath family Ericaceae is found on the territory of Ukraine both in the wild and is widely cultivated in botanical gardens, parks, squares. Leaves and inflorescences are used to treat heart disease, rheumatism, gout, and disorders of the nervous system. The literature data on the chemical composition relate to the presence of aminoacids, organic acids, essential oils, phenolic and triterpene compounds, andromedol derivatives. The aim of the work was to determine the composition and content of fatty and organic acids in the Rhododendron luteum Sweet leaves by gas chromatography-mass spectrometry. The object of the study was the leaves of Rhododendron luteum Sweet, collected on the territory of the botanical garden of H. S. Skovoroda Kharkiv National Pedagogical University in 2019. Gas chromatography - mass spectrometric study of the component composition and content of fatty and organic acids in the leaves of the studied plant on an Agilent Technologies 6890 chromatograph with a 5973 mass spectrometric detector was carried out. In Rhododendron luteum Sweet leaves 37 substances were found and identified. Fatty acids were represented by 17 compounds. Among saturated fatty acids, palmitic acid (3 276.2 mg/kg) was prevailed, among monounsaturated – oleic (736.78 mg/kg), among polyunsaturated – linolenic (1 617.65 mg/kg). The smallest amounts contained heneukocylic (46.33 mg/kg), lauric (58.17 mg/kg) and caproic (68.17 mg/kg) acids, which belong to saturated fatty acids. The presence of 20 organic acids in the raw material was also established: citric (8 680.30 mg/kg) and iso-citric (4 106.85 mg/kg) acids dominated in terms of quantitative content; oxalic (1 685.65 mg/kg) and malic (1 310.82 mg/kg) acids were contained in significant amounts. Among the derivatives of hydroxybenzoic acid, the greatest amount was presented by gentisic (187.76 mg/kg) acid, among the derivatives of hydroxycinnamic acids – p-coumaric (312.62 mg/kg). For the first time, the qualitative composition and quantitative content of fatty and organic acids in Rhododendron luteum Sweet leaves was studied by gas chromatography with a mass spectrometric detector. The obtained results indicate the prospects for further study of raw materials to develop drugs with membrane stabilizing and anti-inflammatory activity.

scholarly journals The study in cockscomb mineral composition

2021 ◽  
Vol 14 (2) ◽  
pp. 194-199
Author(s):  
A. S. Deyneka ◽  
V. V. Protska ◽  
I. O. Zhuravel ◽  
O. A. Kyslychenko ◽  
V. Yu. Kuznietsova
Keyword(s):  
Heavy Metals ◽  
Mineral Composition ◽  
Large Range ◽  
Mineral Elements ◽  
Raw Material ◽  
Plant Roots ◽  
Qualitative Composition ◽  
Quantitative Content ◽  
Nitrogen Containing Compounds

Cockscomb (Сelosia cristata (L.) Kuntze) is grown in Ukraine mostly as a decorative plant. According to literature, it contains phenolic, terpenic, steroid, and nitrogen-containing compounds, showing a large range of biological activity. The aim of the work was to study qualitative composition and determination of quantitative content of mineral elements in cockscomb roots, stems, leaves, flowers, and seeds. Materials and methods. The mineral composition of cockscomb raw material was studied by atomic absorption spectroscopy. Results. In cockscomb roots, stems, leaves, flowers, and seeds 19 mineral elements were identified and determined. The bulk of mineral elements was accumulated in cockscomb leaves – 11580.54 ± 289.51 μg/100 g. In all samples of the herb potassium dominated within the limits of 1125.00 ± 28.12 μg/100 g to 7000.00 ± 175.00 μg/100 g. Prevailing microelements were iron in plant roots and seeds (210.00 ± 5.25 μg/100 g and 81.00 ± 2.02 μg/100 g respectively), aluminum in leaves and flowers (215.00 ± 2.25 μg/100 g and 44.00 ± 1.10 μg/100 g respectively), zinc in stems (5.70 ± 0.14 μg/100 g). Besides, such microelements as zinc (53.00 ± 1.33 μg/100 g) and strontium (21.20 ± 0.53 μg/100 g) were mostly accumulated in roots, manganese (27.00 ± 0.68 μg/100 g) in leaves, copper (2.00 ± 0.05 μg/100 g) in flowers. The content of heavy metals was within the limits stipulated in Ukrainian Pharmacopoeia. Conclusions. The obtained results will be used in the standardization of cockscomb raw material and in the development of medicines on the basis of this raw material.

DETERMINATION OF FATTY ACIDS COMPONENTS COMPOSITION IN THYMUS X CIRIODORUS VAR. «SILVER QUEEN» HERBAL RAW MATERIAL

Fitoterapia ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 55-58
Author(s):  
J. M. Steshenko ◽  
◽  
O. V. Мazulin ◽  
G. P. Smoylovska ◽  
G. V. Mazulin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Raw Material

Solvent Extraction and GC-MS Analysis of Sesame Seeds for Determination of Bioactive Antioxidant Fatty Acid/Fatty Oil Components

Drug Research ◽  
2017 ◽  
Vol 68 (06) ◽  
pp. 344-348 ◽  
Author(s):  
Abdul Qadir ◽  
Athar Ali ◽  
Muhammad Arif ◽  
Abdulmohsen Al-Rohaimi ◽  
Satya Singh ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Palmitic Acid ◽  
Unsaturated Fatty Acids ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Sesame Seeds ◽  
Fatty Oil ◽  
Oleoyl Chloride

AbstractThe seed kernels of Sesamum indicum L. (family: Pedaliaceae) were extracted with ethanol and yield of components determined by Gas Chromatography/Mass Spectrometry (GC/MS). The free radical scavenging activities of ethanolic extract against1, 1-Diphenyl-2-picrylhydrazyl (DPPH) were determined by UV spectrophotometer at 517 nm. Phytochemical screening revealed the presence of numerous bioactive compounds including steroids, phenolic, terpenoids, fatty acids and different types of ester compounds. The ethanolic extract was purified and analyzed by GC MS.The prevailing compounds found in ethanolic extract were Carvacrol (0.04%),Sesamol (0.11%), 4-Allyl-2-methoxy-phenol(0.04%),Palmitic acid (1.08%), cis-9-Hexadecenal (85.40%), Lineoleoyl chloride (0.52%), Palmitic acid β-monoglyceride (0.40%), Dihydro-aplotaxene (0.61%), Oleoyl chloride (1.11%), (+)-Sesamin (4.73%), 1,3-Benzodioxole, 5-[4-(1,3-benzodioxol-5-yloxy)tetrahydro-1 H,3 H-furo [3,4-c]furan-1-yl], [1 S-(1,3,4,6α.), (2.01%)], 6-Nitrocholest-5-en-3-yl acetate (0.22%), Ergost-5-en-3β-ol (2.35%) and 24-Propylidenecholesterol (0.16%). The presence ofsaturated and unsaturated fatty acids in ethanolicextract justifies the use of this plant to treat many ailments in folk and traditional medicine. Ethanolic extract have shown significant antioxidant activity(IC50120.38±2.8 µg/ml). The presence of phenolic (Sesamol), lignin (Sesamin) compounds and unsaturated fatty acids are reported as possible contributor for antioxidantactivity of seed extract.

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