scholarly journals SECONDARY METABOLITES PRODUCED BY MARINE BACTERIRUM MICROMONOSPORA SP. (G068)

2018 ◽  
Vol 54 (2C) ◽  
pp. 465
Author(s):  
Cao Duc Tuan
Keyword(s):  
Escherichia Coli ◽  
Secondary Metabolites ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Culture Broth ◽  
Reported Data ◽  
Quang Ninh

Nine compounds (1-9) were isolated and characterized from the culture broth of the marinebacteria Micromonospora sp. (strain G068), which was isolated from sediment collected at CoTo – Quang Ninh. Their structures were determined by spectroscopic analysis including MS, 1DNMR and 2D NMR, as well as by comparison with reported data in the literature. Allcompounds were evaluated for their antimicrobial activities against a panel of clinicallysignificant microorganisms. Compounds 1, 6, and 9 selectively inhibited Escherichia coli withMIC values of 128, 128 and 64 μg/mL, respectively.

New Metabolites from Penicillium Thymicola IBT 5891

2017 ◽  
Vol 41 (2) ◽  
pp. 95-97 ◽  
Author(s):  
Lie-Feng Ma ◽  
Yang Zheng ◽  
Hao-Ying Qian ◽  
Yuan Wang ◽  
Wei-Guang Shan ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Culture Broth ◽  
Esi Ms ◽  
New Metabolites

Two new secondary metabolites, fumiquinazoline T and thymipyrone A, were obtained from the culture broth of marine-derived Penicillium thymicola IBT 5891. The known compounds, fumiquinazoline F and J, alantrypinone and pestafolide A, were also isolated from this strain. Their structures were elucidated on the basis of extensive spectroscopic analysis, including HR-ESI-MS, IR, 1D- and 2D-NMR.

scholarly journals Novel Substituted Thiophenes and Sulf-Polyacetylene Ester from Echinops ritro L.

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 805 ◽  
Author(s):  
Liang-Bo Li ◽  
Guang-Da Xiao ◽  
Wei Xiang ◽  
Xing Yang ◽  
Ke-Xin Cao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Spectroscopic Analysis ◽  
Inhibitory Concentration ◽  
2D Nmr ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Whole Plant

Three new substituted bithiophenes (1–3), and one new sulf-polyacetylene ester, ritroyne A (16) were isolated from the whole plant of Echinops ritro together with twelve known substituted thiophenes. The structures were elucidated on the basis of extensive spectroscopic analysis including 1D and 2D NMR as well as MS. Furthermore, the absolute configuration of ritroyne A (16) was established by computational methods. In bioscreening experiments, four compounds (2, 4, 12, 14) showed similar antibacterial activity against Staphylococcus aureus ATCC 2592 with levofloxacin (8 µg/mL). Five compounds (2, 4, 9, 12, 14) exhibited antibacterial activities against Escherichia coli ATCC 25922, with minimum inhibitory concentration (MIC) values of 32–64 µg/mL. Three compounds (2, 4, 12) exhibited antifungal activities against Candida albicans ATCC 2002 with MIC values of 32–64 µg/mL. However, compound 16 did not exhibit antimicrobial activities against three microorganisms.

scholarly journals A New 3-Prenyl-2-flavene and Other Extractives from Baphia massaiensis and Their Antimicrobial Activities

2018 ◽  
Vol 13 (4) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Ngonye Keroletswe ◽  
Runner R. T. Majinda ◽  
Ishmael B. Masesane
Keyword(s):  
Escherichia Coli ◽  
2D Nmr ◽  
Antimicrobial Activities ◽  
Spectroscopic Techniques ◽  
Good Activity ◽  
Gram Negative ◽  
E Coli ◽  
Chromatographic Techniques ◽  
Inhibition Zones ◽  
First Time

One new 3-prenyl-2-flavene, named baphiflavene A, 1, and eleven known compounds, 2-12, were isolated and reported for the first time from Baphia massaiensis using several chromatographic techniques. Their structures were elucidated using different spectroscopic techniques; 1D and 2D-NMR, HRMS, GC-MS, UV/Vis, FTIR and by comparison with literature data. The isolates were tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Candida albicans to establish their preliminary antimicrobial activities. The results revealed that compound 1 had moderate activities against both Gram positive ( B. subtilis and S. aureus) and Gram negative ( E. coli and P. aeruginosa) bacteria, and good activity against C. albicans with inhibition zones of 10–23 mm (compared to 19 mm for chloramphenicol and miconazole standards). To the best of our knowledge, this is the first phytochemical work reported on Baphia massaiensis.

scholarly journals Two New Piperazine-Triones from a Marine-Derived Streptomycetes sp. Strain SMS636

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 186 ◽  
Author(s):  
Xiuli Xu ◽  
Jiahui Han ◽  
Rui Lin ◽  
Steven Polyak ◽  
Fuhang Song
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Secondary Metabolites ◽  
Deep Sea ◽  
Spectroscopic Analysis ◽  
Inhibitory Concentration ◽  
2D Nmr ◽  
Antibacterial Activities ◽  
Bacillus Calmette Guerin ◽  
Methicillin Resistant

Two new piperazine-triones lansai E and F (1, 2), together with four known secondary metabolites lansai D (3), 1-N-methyl-(E,Z)-albonoursin (4), imidazo[4,5-e]-1,2,4-triazine (5), and streptonigrin (6) were isolated from a deep-sea-derived Streptomycetes sp. strain SMS636. The structures of the isolated compounds were confirmed by comprehensive spectroscopic analysis, including HRESIMS, 1D and 2D NMR. Compound 4 exhibited moderate antibacterial activities against Staphylococcus aureus and methicillin resistant S. aureus (MRSA) with Minimum Inhibitory Concentration (MIC) values of 12.5 and 25 μg/mL, respectively. Compound 6 displayed significant antibacterial activities against S. aureus, MRSA and Bacillus Calmette-Guérin (BCG) with MIC values of 0.78, 0.78 and 1.25 μg/mL, respectively.

scholarly journals ANTIMICROBIAL POTENTIALS OF SOIL FUNGI METABOLITES, MOLECULAR DYNAMICS AND THEIR 3D PROTEIN STRUCTURAL PREDICTION USING BIOINFORMATICS TOOL

2020 ◽  
pp. 20-26
Author(s):  
MAJOLAGBE O. N. ◽  
AINA D. A. ◽  
OMOMOWO I. O. ◽  
THOMAS A.
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Protein Structure ◽  
Secondary Metabolites ◽  
Soil Fungi ◽  
3D Structure ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
3D Protein Structure ◽  
Klebsiella Spp

Objective: To determine the antimicrobial potentials of secondary metabolite of soil fungi and predict their 3D structure and molecular identity. Methods: Pure soil fungi were isolated from soil samples and cultured under submerged fermentation (Smf) for their metabolites using Potato Dextrose Agar and Broth. The secondary metabolites of the isolated fungi were obtained intracellularly after 21 d of incubation in a rotary shaker incubator. The antimicrobial potentials of the metabolites were investigated against four (4) clinical isolates, namely: Staphylococcus aureus, Klebsiella spp, Candida albicans and Escherichia coli. These soil fungi were further characterized to the molecular level and their evolutionary relationships established using bioinformatics tools. Protein structure of each of the fungi isolates was predicted using PHYRE-2. Results: Out of all the soil fungi isolated, the metabolite of Aspergillus aculeatus showed the highest antimicrobial activities against Staphylococcus aureus (23.00±2.34 mm), Escherichia coli (9.00±1.44 mm) and Klebsiella spp (24.00±3.45 mm). The 3D protein structure predicted showed that each of the organisms consists of different amino-acid compositions such as: serine, tyrosine, proline, arginine, glycine, phenylalanine leucine with other notable biological properties. Conclusion: The work revealed that secondary metabolites of the isolated fungi carry an important role in combating infectious agents thereby, providing roadmaps for the biosynthesis of many synthetic and semi-synthetic drugs and bio-products which are environmentally friendly.

scholarly journals Ring B Aromatic Steroids from an Endophytic Fungus, Colletotrichum sp.

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Wen Zhang ◽  
Siegfried Draeger ◽  
Barbara Schulz ◽  
Karsten Krohn
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Bacillus Megaterium ◽  
Endophytic Fungus ◽  
Spectroscopic Analysis ◽  
Chemical Diversity ◽  
Chlorella Fusca ◽  
Microbotryum Violaceum ◽  
Reported Data ◽  
Insight Into

The new (22E,24R)-3-acetoxy-19(10→6)-abeo-ergosta-5,7,9,22-tetraen-3β-ol (1) and the known (22E,24R)-19(10→6)-abeo-ergosta-5,7,9,22-tetraen-3β-ol (2), two interesting ergosteroids with rare aromatized ring B, together with seven known derivatives, namely (22E,24R)-ergosta-5,7,22-trien-3β-ol (3), (22E,24R)-ergosta-4,7,22-trien-3-one (4), (22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one (5), (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (6), (22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol (7), (22E,24R)-6-acetoxy-ergosta-7,22-dien-3β,5α,6β-triol (8), and (22E,24R)-3,6-diacetoxy-ergosta-7,22-dien-3β,5α,6β-triol (9), were isolated from Colletotrichum sp., an endophytic fungus isolated from Ilex canariensis from Gomera. The structures of these compounds were elucidated by detailed spectroscopic analysis, comparison with reported data, and chemical interconversion. The isolation of these metabolites not only displays a beautiful array of chemical diversity, but also gives insight into the biosynthetic interconnections. Preliminary studies showed antimicrobial activity of these compounds against the fungus Microbotryum violaceum, the alga Chlorella fusca, and the bacteria Escherichia coli and Bacillus megaterium.

Phytochemical, antibacterial, antioxidant and cytoxicity investigation of Tarenna grandiflora

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Jean Francois Zeutsop ◽  
Raymond Ngansop Nono ◽  
Marcel Frese ◽  
Jean Rodolphe Chouna ◽  
Bruno Ndjakou Lenta ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Cell Line ◽  
Spectroscopic Analysis ◽  
Antioxidant Activities ◽  
2D Nmr ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Phytochemical Investigation ◽  
Antioxidant And Antimicrobial Activities

AbstractThe phytochemical investigation of Tarenna grandiflora led to the isolation of 18 known compounds of which were four flavones, three anthraquinones, one phenyl propanoic derivative, five triterpenoids, four steroids and a mixture of glucose. Luteolin (1) and soranjidiol (6) were allylated and/or prenylated to give four new semisynthesized derivatives which were fully characterized as 7,3′,4′-O-triallylluteolin (1a), luteolin-7,3′,4′-O-triprenyls (1b), luteolin-5,7,3′,4′-O-tetraprenyls (1c) and 6-O-allylsoranjidiol (6a). Their structures were established using spectroscopic analysis including 1D, 2D NMR and MS data. The cytotoxic, antioxidant and antimicrobial activities of extracts, fractions, isolated compounds and semi-synthesized derivatives were evaluated. The petroleum ether and EtOAc extracts exhibited good cytotoxic potency on KB-3-1 cell line with IC50 of >0.1 and 0.025 mg/mL respectively, while compounds 1b and 11 were the most active (IC50 > 0.0001 M). Compounds 1 and 3 showed the best antioxidant activities (45.5 and 55.8 µM); while compounds 9 and 12 showed the best antibacterial activities with MICs values ranges from 8.55 to 132 µM.

scholarly journals Isolation of Sesquiterpenoids and Steroids from the Soft Coral Sinularia brassica and Determination of Their Absolute Configuration

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 523
Author(s):  
Giang Nam Pham ◽  
Da Yeun Kang ◽  
Min Ju Kim ◽  
Se Jong Han ◽  
Jun Hyuck Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Leishmania Donovani ◽  
Spectroscopic Analysis ◽  
Soft Coral ◽  
Antimicrobial Effect ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Polyhydroxylated Steroids

Two undescribed rearranged cadinane-type sesquiterpenoids (1–2), named sinulaketol A-B, together with one new chlorinated steroid (3), one new gorgosterol (4), one known sesquiterpene (5), one known dibromoditerpene (6) and two known polyhydroxylated steroids (7–8) were isolated from the soft coral Sinularia brassica. The structures of these compounds were established by extensive spectroscopic analysis, including HRESIMS, 1D, and 2D NMR spectroscopy. Their absolute configurations were also determined by the ECD calculations and DP4+ probability analysis. Antileishmanial activity of compounds 1–8 was evaluated in vitro against the amastigote forms of Leishmania donovani, in which compounds 3, 6, and 7 inhibited the growth of L. donovani by 58.7, 74.3, 54.7%, respectively, at a concentration of 50 μM. Antimicrobial effect of the isolated compounds were also evaluated against Candida albicans, Staphylococcus aureus, and Escherichia coli. Compound 6, a brominated diterpene, exhibited antimicrobial effect against S. aureus.

scholarly journals Chemical constituents of leaves Dialium cochinchinense Pierre

2021 ◽  
Vol 4 (3) ◽  
pp. 1131-1136
Author(s):  
Vu Thi Huyen ◽  
Doan Thi Thuy Ai ◽  
Nguyen Thi Hien
Keyword(s):  
Secondary Metabolites ◽  
Nmr Spectra ◽  
Spectroscopic Analysis ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Tropical Regions ◽  
First Report ◽  
Isolation And Characterization

The genus Dialium belongs to the Caesalpinioideae family, consisting of approximately 30 species distributed in the tropical regions. Secondary metabolites from the  Dialium genus have been reported to exhibit various biological activities including antioxidant, cytotoxicity and antimicrobial activities. This work describes the isolation and characterization of five compounds from the leaves of Dialium cochinchinense Pierre. Their structures were established by spectroscopic analysis, including MS and NMR spectra. Accordingly, the isolated compounds were identified to be lupeone (1), b-sitostenone (2), β-sitosterol (3), daucosterol (4), and dihydrokaempferide (5). To the best of our knowledge, this is the first report of the isolation of compounds 1 and 5 from the genus Dialium.

scholarly journals Perylene Derivatives Produced by Alternaria alternata, an Endophytic Fungus Isolated from Laurencia Species

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Shu-Shan Gao ◽  
Xiao-Ming Li ◽  
Bin-Gui Wang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Aspergillus Niger ◽  
Alternaria Alternata ◽  
Endophytic Fungus ◽  
Spectroscopic Analysis ◽  
Algal Species ◽  
Antimicrobial Activities ◽  
Perylene Derivatives

Two new perylene derivatives, 7-epi-8-hydroxyaltertoxin I (1) and 6-epi-stemphytriol (2), along with two known compounds stemphyperylenol (3) and altertoxin I (4) were isolated from Alternaria alternata, a marine endophytic fungus derived from an unidentified algal species of the genus Laurencia. Structures of compounds 1-4 were determined on the basis of detailed spectroscopic analysis, as well as by comparison with literature reports. The antimicrobial activities of compounds 1 and 3 against Staphylococcus aureus, Escherichia coli, and Aspergillus niger were evaluated; neither showed obvious activity.

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