Histologic Analysis of the Oral Mucosa Lining Osseointegrated Implant Cover Screws: A Study in Humans

2010 ◽  
Vol 36 (1) ◽  
pp. 3-10
Author(s):  
André Alan Nahas ◽  
Paula Dechichi ◽  
Denildo de Magalhães ◽  
Andreia Espíndola Vieira

Abstract Osseointegrated dental implants are inserted into the alveolar ridge, and for them to function as tooth replacements, the surrounding tissues need to adapt to them. Just as with teeth, dental implants traverse the oral mucosa and have access to the contaminated environment of the oral cavity. Therefore, periodontal and peri-implant tissues are important for establishing a protective barrier. The aim of the present study was to perform a histologic analysis of the mucosa surrounding osseointegrated implant cover screws. For this study, 17 mucosal specimens were obtained from 12 patients during the second surgical session for implant exposure to the oral environment. After histologic preparation, specimens were sectioned perpendicularly to the mucosal surface to a thickness of about 3 µm, stained with 1% toluidine blue, and examined under light microscopy. All specimens showed a keratinized, stratified, squamous epithelium with well-defined strata. In the lamina propria, unorganized dense connective tissue was noted in the reticular layer, and in 4 samples, a chronic inflammatory infiltrate was seen in this region. The papillary layer presented tall connective papillae consisting of loose connective tissue. The results of this study confirm the hypothesis that the mucosa that conceals osseointegrated implant cover screws has the same morphologic characteristics as the alveolar masticatory mucosa. Furthermore, clinical conditions of normality in peri-implant tissues may not coincide with situations of histologic normality.

2020 ◽  
Vol 21 (14) ◽  
pp. 5043
Author(s):  
Martin Lissek ◽  
Martin Boeker ◽  
Arndt Happe

This systematic review aimed to assess the effectiveness of xenogeneic collagen matrices (XCMs) and acellular dermal matrices (ADMs) in comparison to connective tissue grafts (CTGs) for the augmentation of oral mucosa around dental implants. MEDLINE and the Web of Science were searched for clinical studies that compared substitute materials for the augmentation of oral mucosa to the subepithelial connective tissue graft around dental implants during or after implantation. The review was conducted according to the recommendations of the PRISMA statement. From an initial search result set of 1050 references, seven articles were included in the review. The study designs were heterogeneous, so no meta-analysis could be performed. Both the CTG and either type of substitute material resulted in increased mucosal thickness. Four studies showed no significant difference, while three demonstrated a significant difference, favoring the CTGs over alternative materials. Soft tissue augmentation around dental implants is a safe procedure and leads to thicker mucosal tissue. The subepithelial connective tissue graft can still be regarded as the gold standard, but substitute materials may be an acceptable alternative in some situations, such as for pain-sensitive patients, among inexperienced surgeons, and for sites with an already thick biotype.


Author(s):  
J. S. Alexandrawicz

A specimen of the red mullet (Mullus surmuletus L.), caught in Plymouth waters in October 1949, was found to have tumours projecting externally on the pectoral fins. They were spherical or elliptical in shape, the largest being 20 mm. long. On the suggestion of Mr G. A. Steven some of these outgrowths were cut out and given to the writer for microscopic examination.When examined fresh they appear to consist of small, spherical, cyst-like bodies of various diameters, but there was no obvious clue as to their nature. After fixing in Bouin and sectioning, they are seen to be composed of cells of various sizes (PI. I, figs. 1–4). It can be seen that these cells lie close to one another, leaving only a little space for the embedding connective tissue which is characterized by the abundance of its own small cells. The blood vessels met with in this tissue contain fish erythrocytes. The whole is covered by stratified squamous epithelium (PI. I, fig. 2).


2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Nurcan Tezci ◽  
Suleyman Emre Meseli ◽  
Burcu Karaduman ◽  
Serap Dogan ◽  
Sabri Hasan Meric

Background. Oral fibromas are benign, asymptomatic, smooth surfaced, firm structured tumoral lesions that originate from gingival connective tissue or periodontal ligament. Histologically, they are nodular masses characterized by a dense connective tissue, surrounded by stratified squamous epithelium.Case Report. This case report includes the clinical, radiographical, and histological findings and periodontal treatment of a 38-year-old female patient having painless swelling on the gingiva. Intraoral examination revealed a fibrotic, sessile, smooth surfaced gingival overgrowth interdentally between the teeth #13 and #14. Radiographical findings were normal. Initial periodontal treatment (IPT) was applied including oral hygiene instructions, scaling, and root planing. Following IPT, the lesion (0.7 × 0.6 × 0.4 cm) was excised and examined histopathologically. Subsequently, flap operation was performed to have an access to alveolar bone. Surgical site was reconstructed with free gingival graft obtained from hard palate. Hematoxylin-eosin stained sections revealed a nodular mass composed by dense collagen fibers in lamina propria covered by a stratified squamous epithelium, which were consistent with fibroma. Gingival healing was uneventful and without any recurrence during the 12-month follow-up.Conclusions. In order to achieve optimal functional and aesthetical outcomes, free gingival graft can be used for the reconstruction of the wound site after the excision of the fibroma.


2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Aliman Irfandi ◽  
Cut Dahlia Iskandar ◽  
Zainuddin Zainuddin ◽  
Dian Masyitha ◽  
Fitriani Fitriani ◽  
...  

Domestical catfish is grouped into omnivora, although the fish is tended into carnivora.  The aim of the present study was to investigate histology of alimentary tract of domestical catfish (Clarias batrachus). In order to determine alimentary tract of domestical catfish, oesophagus, gastric and intestines is investigated by Haematoxilin Eosin. Histology exploration was performed each layer of alimentary tract’s wall consist of lamina epithelia, propria, and muscularis mucosa. Collagen connective tissue was developed tunica submucosa. The muscle of tunica muscularis was performed circular and elongated. Dense connective tissue and blood vessels was located in the tunica serosa. Histology exploration revealed has stratified squamous epithelium, mucosit cells, eosinophilic club cells on the lamina epithelia, and taste buds on the lamina propria in oesophagus. Gastric was establised simple prismatic cells, and glands of gastric on the lamina propria. Furthermore intestine was showed villi and goblet cells. There were not showed Brunner and Liberkhun gland. In conclusion, histology of alimentary tract was revealed from tunica mucosa, submucosa, and serosa.


Development ◽  
1961 ◽  
Vol 9 (3) ◽  
pp. 370-384
Author(s):  
C. B. McLoughlin

It is well established that in the developing chick the underlying mesenchyme initiates the appearance of specific epidermal derivatives, e.g. feathers (Sengel, 1956), claws (Cairns & Saunders, 1954), and the preen gland (Gomot, 1958). On the other hand, it is not yet known to what extent the epidermis is independent of mesenchymal intervention for its basic differentiation into a stratified, squamous epithelium. Sobel's (1958) work on the 8-day chick pituitary suggests that the differentiation and multiplication of certain epithelial cells cannot proceed in the absence of mesenchymal elements. She found that the isolated epithelial cells of the hypophysial rudiment survived but were unable to differentiate or multiply; when associated with perichondrial fibroblasts, however, they resumed mitosis and produced typical α and β cells. In the first part of the present investigation, experiments were made to see whether the embryonic epidermis, like the hypophysial epithelium, requires the resence of fibroblasts to enable it to grow and differentiate, or whether it can proliferate, acquire its characteristic squamous structure and keratinize, when isolated and cultivated in the absence of connective tissue.


2014 ◽  
Vol 6 (3) ◽  
pp. 134-136
Author(s):  
Nilamadhab Prusty

ABSTRACT Epidermoid cyst occurring within the tongue is rare. A 5-year- old male child was brought to outpatient department (OPD) with a tongue mass which was gradually increasing in size. There was associated difficulty in speech and mastication as the swelling increased in size. Intraoral examination revealed moderately tender, fluctuant and enlarged tongue. A diagnosis of dermoid cyst was made and the patient was booked for surgery. Excision of the cyst was done under general anesthesia. Postoperative histopathology was done. The histopathological findings confirm the diagnosis of an epidermoid cyst, characterized by the presence of: (i) a cyst cavity lined by stratified squamous epithelium with keratinization on the surface; and (ii) connective tissue with a mild inflammation. The proposed treatment was considered successful as the case was solved and there was no recurrence. How to cite this article Naik C, Prusty N. Epidermoid Cyst of Tongue: A Case Presentation. Int J Otorhinolaryngol Clin 2014;6(3):134-136.


1971 ◽  
Vol 49 (2) ◽  
pp. 241-248 ◽  
Author(s):  
F. Ramprashad ◽  
S. Corey ◽  
K. Ronald

The gross and microscopic structure of the auditory meatus of the harp seal (Pagophilus groenlandicus) is described from dissections and serial sections. The meatus bends ventrally from the oval external orifice, posteroventrally, ventrally, and finally medially into the cranium. The ventrally directed wall of the meatus is membranous, the posteroventral and ventrally directed wall is incompletely wrapped by cartilage, and the medially directed wall is bony. The areas between sections of the cartilaginous wall ate completed by connective tissue. The meatus is closed internally by the intrinsic helicis and antitragicus auricular muscles. The external orifice is closed by the superficial auricular muscles. The auditory meatus is lined with stratified squamous epithelium, sebaceous glands, and ceruminous glands. Longitudinal blood sinuses and elastic fibers in the hypodermis may function as a pressure regulating device during diving. Blood sinuses are most prominent in the bony region of the meatus and extend into the tympanic sulcus of the pars tensa. Blood sinuses engorged with blood and a closed meatus may enhance transmission of sound to the tympanic membrane when the seal is under water.


2018 ◽  
Vol 66 (7) ◽  
pp. 531-538
Author(s):  
Mechthild Stoeckelhuber ◽  
Friedrich Feuerhake ◽  
Christoph Schmitz ◽  
Klaus-Dietrich Wolff ◽  
Marco R. Kesting

Surfactant proteins in different glandular structures of the oral cavity display antimicrobial activity for protection of invading microorganisms. Moreover, they are involved in lowering liquid tension in fluids and facilitate secretion flows. Numerous investigations for studying the occurrence of surfactant proteins in glandular tissues were performed using different methods. In the oral cavity, minor salivary glands secrete saliva continuously for the maintenance of a healthy oral environment. For the first time, we could show that infantile labial glands show expression of the surfactant proteins (SP) SP-A, SP-B, SP-C, and SP-D in acinar cells and the duct system in different intensities. The stratified squamous epithelium of the oral mucosa revealed positive staining for SPs in various cell layers.


2016 ◽  
Vol 54 (201) ◽  
pp. 43-45
Author(s):  
Choubarga Naik ◽  
Nilamadhab Prusty

Epidermoid cyst occurring within the tongue is rare. A 5 year old male child was brought to OPD with a tongue mass which was gradually increasing in size. There was associated difficulty in speech and mastication as the swelling increased in size. Intraoral examination revealed moderately tender, fluctuant and enlarged tongue. A diagnosis of dermoid cyst was made and the patient was booked for surgery. Excision of the cyst was done under general anaesthesia. Post-operative histopathology was done. The histopathological findings confirm the diagnosis of an epidermoid cyst, characterized by the presence of: (I) a cyst cavity lined by stratified squamous epithelium with keratinization on the surface; and (II) connective tissue with a mild inflammation. The proposed treatment was considered successful as the case was solved and there was no recurrence. Keywords: dermoid; epidermoid cyst;tounge. | PubMed


Author(s):  
E. J. Kollar

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.


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