TOXICITY OF ZINC TO HETEROTROPHIC BACTERIA FROM A TROPICAL RIVER SEDIMENT

Polycyclic aromatic hydrocarbon (PAH) degrading potential of bacteria isolated from Iko River sediment, Akwa Ibom State, Nigeria was investigated. The mean total heterotrophic bacteria obtained from the sediment samples was 6.4 × 10 cfu/g while 9.8 x 103 cfu/g hydrocarbon utilizing bacteria was recorded. Preliminary screening of the hydrocarbonoclastic bacterial isolates revealed that among the 12 bacterial isolates, Pseudomonas aeruginosa, Bacillus subtilis, Alcaligenes sp exhibited the strongest ability to utilize crude oil. The result also revealed Bacillus subtilis and Pseudomonas aeruginosa as the best PAH degraders. A higher microbial cells count in the 2-ring PAH (naphthalene) supplemented –MSM was recorded. The levels of attenuance however varied with the test organism and were accompanied by fluctuations but decreasing pH levels and slight changes in temperature of the culture medium. In vitro degradation study carried out for the 21 days showed that the degradation of PAH when augmented with Pseudomonas aeruginosa and Bacillus subtilis respectively was faster than when un-augmented. The PAH content was reduced by Pseudomonas aeruginosa and Bacillus subtilis from 10.42 to 9.03 and 9.56 respectively. The hydrocarbon degradation by augmented cultures of Pseudomonas aeruginosa and Bacillus subtilis implies that bioaugmentation can be harnessed for bioremediation purposes.

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Fungicidal and Bactericidal Activity of the Alkyl-Substituted Guanidine-Containing Oligomers

Mikrobiolohichnyi Zhurnal ◽

10.15407/microbiolj82.06.054 ◽

2020 ◽

Vol 82 (6) ◽

pp. 54-63

Author(s):

M.Ya. Vortman ◽

◽

Yu.B. Pysmenna ◽

A.I. Chuenko ◽

D.R. Abdulina ◽

...

Keyword(s):

Heterotrophic Bacteria ◽

Trichoderma Viride ◽

Rhodococcus Erythropolis ◽

Biological Action ◽

Molar Ratio ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Alkyl Bromides ◽

Base Form ◽

Two Stages

Biocides are widely used in medicine and various industries to protect against a number of harmful microorganisms. Organic quaternary ammonium and guanidine-containing compounds, the biological action of which is based on membrane-toxic properties, are used as bactericidal preparations. The aim of this work was to study the bactericidal and fungicidal activities of the synthesized oligomeric alkylsubstituted guanidinium bromides with different radicals -C3H7, -C7H15, -C10H21, against different isolates of heterotrophic bacteria and microscopic fungi. Methods. The synthesis of alkyl-substituted guanidiniumcontaining oligomers was performed in two stages. In the first stage, alkyl-substituted guanidine was obtained by the reaction of guanidine, previously converted by alkali from the salt form to the base form by the base and alkyl bromides (Alk=-C3H7 (propyl), -C7H15 (heptyl), -C10H21 (decyl)) in methanol at a temperature of 50°C and a molar ratio of 1:1. The second carried out the reaction between aromatic oligoepoxide DER-331 and alkyl-substituted guanidine in methanol at a temperature of 50°C for 2–3 hours and a molar ratio of 1:2. Bacteria were grown on meat-peptone agar for 48 hours at a temperature of 28±2°С. Test cultures of micromycetes were cultured on agar beer wort (6°B), incubated for 14 days in a thermostat at a temperature of 28±2°C. Antimicrobial activity of newly synthesized alkyl-substituted guanidinium-containing oligomers was determined by standard disco-diffusion method (method of disks on agar) and fungicidal activity was determined by the method of holes in agar. Results. Oligomeric alkylsubstituted guanidinium bromides with different radicals composed -C3H7, -C7H15, -C10H21- synthesized by the reaction of guanidine alkyl bromides with aromatic oligoepoxydes. It was found that alkyl-substituted guanidinium-containing oligomers at a concentration of 1–3% inhibited the growth of Escherichia coli 475, Pseudomonas aeruginosa 465, Klebsiella pneumonia 479, Pseudomonas pseudoalcaligenes 109, Staphylococcus aureus 451, E. faecalis 422, Rhodococcus erythropolis 102, Bacillus subtilis 138 and most of the studied micromycetes – Aureobasidium pullulans F-41430, Paecilomyces variotii F-41432, Penicillium funiculosum F-41435, Penicillium ochrochloron F-41431, Scopulariopsis brevicaulis F-41434, Trichoderma viride F-41437, Candida albicans F-41441, Aspergillus flavus F-41442, Aspergillus niger F-41448, Penicillium sp. F-41447. Conclusions. Antimicrobial and fungicidal properties significantly depend on the length of the alkyl radical, with increasing of its length the diameter of the zone of bacterial and micromycetes growth retardation increases.10.15407/microbiolj82.06.054

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MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v5i1.7769 ◽

2013 ◽

Vol 5 (1) ◽

Author(s):

Lies Indah Sutiknowati

Keyword(s):

Pathogenic Bacteria ◽

Heterotrophic Bacteria ◽

Total Coliform ◽

Total Cell ◽

Epifluorescence Microscopy ◽

Coliform Bacteria ◽

Total Density ◽

Shellfish Aquaculture ◽

Microbiological Parameters ◽

Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

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Faculty Opinions recommendation of Occurrence and distribution antibiotic resistance of heterotrophic bacteria isolated from a marine beach.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1023978.285816 ◽

2005 ◽

Author(s):

Marilyn Roberts

Keyword(s):

Antibiotic Resistance ◽

Heterotrophic Bacteria

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Analysis of cultivable heterotrophic bacteria involved in nitrogen cycle in grass carp(Ctenopharyngodon idellus)pond

JOURNAL OF FISHERIES OF CHINA ◽

10.3724/sp.j.1231.2012.28008 ◽

2012 ◽

Vol 36 (10) ◽

pp. 1572 ◽

Cited By ~ 1

Author(s):

Luo-qin FU ◽

Jian-dong SUN ◽

Bin DENG ◽

Quan LIANG ◽

Xiao-ping ZHANG ◽

...

Keyword(s):

Nitrogen Cycle ◽

Grass Carp ◽

Heterotrophic Bacteria ◽

Ctenopharyngodon Idellus

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Oscillating conditions for influencing the composition of mixed biological cultures

Water Science & Technology ◽

10.2166/wst.1998.0640 ◽

1998 ◽

Vol 37 (4-5) ◽

pp. 259-262 ◽

Cited By ~ 2

Author(s):

Bjarne R. Horntvedt ◽

Morten Rambekk ◽

Rune Bakke

Keyword(s):

Growth Rate ◽

Specific Growth ◽

Heterotrophic Bacteria ◽

Bacterial Species ◽

Rate Reduction ◽

Similar Strategy ◽

Sinusoidal Oscillations ◽

Specific Growth Rates ◽

Rate Limiting ◽

Concentration Levels

This paper presents a strategy in which mixed biological cultures are exposed to oscillating concentration levels, to improve the potential for coexistence of desired bacterial species. A mechanistic mathematical model is constructed to investigate and illustrate this strategy. This paper is focused on competition between nitrifying, denitrifying and aerobic heterotrophic bacteria in a CSTR with sludge recycle. For nitrifying and aerobic heterotrophic cultures, the effect of sinusoidal oscillations in DO levels with an amplitude of 1.0 mg/l is a 16% specific growth rate reduction compared to that at a constant DO level. The denitrifiers growth rate is increased by an average of 59%, compared to the constant DO level situation. A similar strategy has been tested in a pilot plant. It is concluded that the influence on specific growth rates is a function of the amplitude of the oscillations. The effects are greatest when concentrations fluctuate around the half saturation concentration of the rate limiting component(s).

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The Microbial Quality of a Wetland Reclamation Facility Used to Produce an Effluent for Unrestricted non-Potable Reuse

Water Science & Technology ◽

10.2166/wst.1999.0612 ◽

1999 ◽

Vol 40 (4-5) ◽

pp. 369-374 ◽

Cited By ~ 2

Author(s):

R. S. Fujioka ◽

A. J. Bonilla ◽

G. K. Rijal

Keyword(s):

Fecal Coliform ◽

Heterotrophic Bacteria ◽

Fecal Indicator ◽

Potable Reuse ◽

Wetland Reclamation ◽

Treated Sewage ◽

Influent Water ◽

Human Viruses ◽

To Receive

An auxiliary Wetland Reclamation Facility (WRF) was constructed to receive stabilization pond treated sewage and further treat it with water hyacinth ponds, chemical flocculation, filtration and ultraviolet light disinfection. This was the first facility in Hawaii which was approved to produce the highest quality reclaimed water using alternative treatment schemes. We assessed the effectiveness of the WRF by monitoring water samples after each of the WRF treatment schemes for five genetically different groups of sewage borne microorganisms (fecal coliform, enterococci, C. perfringens, FRNA phage, total heterotrophic bacteria). The concentrations of all fecal indicator microoganisms, especially FRNA phase were low in the influent water to the WRF indicating that extended pond treatment may be especially effective in removing human viruses from sewage. The WRF treatment scheme was calculated to be able to reduce >99.99% of fecal coliform and therefore was able to produce an effluent meeting the non-potable, unrestricted reuse standard of a geometric means of <1 fecal coliform/100 ml.

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Distribution of nitrifying bacteria in a shallow stream

Water Science & Technology ◽

10.2166/wst.1997.0660 ◽

1997 ◽

Vol 36 (8-9) ◽

pp. 161-166 ◽

Cited By ~ 1

Author(s):

Ivana Jancarkova ◽

Tove A. Larsen ◽

Willi Gujer

Keyword(s):

Field Experiments ◽

Heterotrophic Bacteria ◽

Pollution Source ◽

Nitrifying Bacteria ◽

Channel Water ◽

River Bed ◽

Hydraulic Conditions ◽

Major Floods ◽

Shallow Stream ◽

Stream Bed

A project investigating the dynamics of self-purification processes in a shallow stream is carried out. Effects of the concentration gradient due to the distance to the pollution source, of hydraulic conditions in the river bed and of storm floods on the distribution of nitrifying bacteria were studied with the help of laboratory and field experiments. Nitrifiers density on the surface of the stream bed increased rapidly up to a distance of 300 m from the WWTP indicating possible competition of the nitrifiers with the heterotrophic bacteria close to the WWTP. Afterwards a slight decrease in the downstream direction was observed. In vertical profiles, higher bacterial densities were found at sites with rapid infiltration of channel water to the stream bed than at sites with no exchange between channel water and stream bed water or where stream bed water exfiltrated. A major flood event scoured the nitrifiers nearly totally from the surface of the river bed. Major floods belong so to the most dominant processes controlling self-purification in shallow streams. Minor floods, however, don't scour bacteria in the depth of the stream bed that could then be important for the self-purification processes.

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