scholarly journals Determination of functional and technological properties of beef based on the analysis of color digital images of muscular tissue samples

2018 ◽  
Vol 12 (3) ◽  
Author(s):  
О. Savinok ◽  
N. Azarova ◽  
О. Arsiriy ◽  
А. Nikolenko
Keyword(s):  
Muscle Tissue ◽  
Cold Storage ◽  
Information Technologies ◽  
Digital Images ◽  
Iron Oxidation ◽  
Approximation Error ◽  
Maximum Level ◽  
Coordinate Space ◽  
Muscular Tissue ◽  
Tissue Samples

The paper considers the kinetics of changes in the values of рН and temperature of beef of slaughtered Holstein bull calfs aged 15 months during cold storage. It has been established that the rate of pH decrease during autolytic maturation is greatly influenced by the rate of temperature decrease. This was observed in the two muscles taken as an example – m. Longissimus dorsi and m. Semimembranosus. A method is suggested of analysing digital images of beef muscular tissue samples in the color coordinate space to study the beef’s color characteristics during cold storage. It has been found out that using this method, with second order polynomial fitting, provides a mean-square approximation error of 5.6% on average from the minimum coordinate of the maximum level of the red component of color. This suggests the objectivity of its use to assess the color of the meat. An analytical dependence has been established between the beef color and the term of refrigeration with the use of information technologies. In accordance with it, it has been determined that due to biochemical processes, there takes place intense oxidation of myoglobin, which results in a dark color of the muscle tissue. In the course of time (up to 120 hours of storage), the red color intensity increases. This is accompanied with decomposition of myoglobin forms that have appeared, and with appearance of МbО2. When beef is stored for more than 140 hours, deeper iron oxidation begins, with formation of methyoglobin, and the brightness of the meat decreases. The developed method allows automating registration and processing of images of muscle tissue in real time, increases the productivity of the assessment, and gives an opportunity to obtain reliable objective results about the meat properties during its storage.

scholarly journals CONSISTENT PATTERNS OF POSTMORTAL STRUCTURAL AND BIOCHEMICAL CHANGES IN DIAPHRAGM MUSCULAR TISSUE: IMPORTANCE FOR FORENSIC MEDICAL ESTIMATION OF TIME OF DEATH COMING

2019 ◽  
Vol 19 (1) ◽  
pp. 71-76
Author(s):  
N.S. Konoval
Keyword(s):  
Acid Phosphatase ◽  
Muscle Tissue ◽  
Biochemical Changes ◽  
Diaphragm Muscle ◽  
Time Interval ◽  
Muscular Tissue ◽  
Time Of Death ◽  
Post Mortem ◽  
Time Intervals ◽  
Tissue Samples

The issue on estimating the time of death coming still remains a challenge that requires a lot of questions to be answered, therefore, the development of new scientific methods aimed at overcoming these difficulties is a top priority in solving the problem of estimating the time of death coming. The purpose of this study was to investigate the structural and biochemical changes in the muscle tissue of the diaphragm in the early post-mortem period. The muscular tissue samples of the diaphragm just below the lungs taken from 30 human corpses in the early post-mortem period (in time intervals of 3-13 hours from the onset of death) were investigated. We studied structural and biochemical markers in the homogenates of the muscles of the diaphragm: the content of glycogen, acid phosphatase, lactate, lactate dehydrogenase, lipofuscin, and cholinesterase. It was found out that in the early post-mortem period structural and biochemical changes in the muscle tissue of the diaphragm are characterized by regular morphological biochemical and biophysical changes. In particular, the post-mortem dynamic changes in glycogen, acid phosphatase and lipofuscin levels is most significant; i.e. within the time interval of 3–9 hours from the time of death coming, the assessment of the “cholinesterase / glycogen or acidic phosphatase / glycogen” ratio is the most diagnostically valuable, then after 9 hours the only “cholinesterase / glycogen” ratio is meaningful. The content of glycogen in the homogenates of the diaphragm muscle tissue depending on the time of death coming ranged from (4.765 ± 0.058) mg / g in 3 hours, to (1.883 ± 0.027) mg / g in 13 hours after the death coming, reliably ( p <0.001) differing in time intervals. The inverse pattern was characterized by the acid phosphatase content: (2.748 ± 0.019) u / g in 3 hours and (4.49 ± 0.032) u / g in 13 hours. These markers are the most significant to demonstrate the patterns and can be used as critically important signs in forensic diagnosis.

scholarly journals Development of a Method to Determine Albendazole and Its Metabolites in the Muscle Tissue of Yellow Perch Using High-Performance Liquid Chromatography with Fluorescence Detection

2011 ◽  
Vol 94 (2) ◽  
pp. 446-452 ◽  
Author(s):  
Donglei Yu ◽  
Nathan Rummel ◽  
Badar Shaikh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Muscle Tissue ◽  
Mobile Phase ◽  
Yellow Perch ◽  
High Performance ◽  
Ammonium Acetate ◽  
Hplc Method ◽  
Tissue Samples ◽  
Phase Combination

Abstract An HPLC method was developed for the determination of albendazole (ABZ) and its metabolites, a sulfoxide (ABZSO), a sulfone (ABZSO2), and albendazole-2-aminosulfone (ABZ-2-NH2SO2), from yellow perch muscle tissue with adhering skin. The muscle tissue samples were made alkaline with potassium carbonate and extracted with ethyl acetate, followed by a series of liquidliquid extraction steps. After solvent evaporation, the residue was reconstituted in the initial mobile phase combination of the gradient. The mobile phase consisted of a buffer, 50 mM ammonium acetate (pH 4.0) in 10 methanolwater, and 100 acetonitrile. The gradient was from 20 acetonitrile to 85 acetonitrile. The analytes were chromatographed on an RP Luna C18(2) column and detected by fluorescence with excitation and emission wavelengths of 290 and 330 nm, respectively. The average recoveries from fortified muscle tissue for ABZ (20100 ppb), ABZ-SO (20200 ppb), ABZSO2 (8100 ppb), and ABZ-2-NH2SO2 (20100 ppb) were 85, 95, 101, and 86, respectively, with corresponding CV values of 9, 3, 6, and 4, respectively. Their LOQ values were 10, 10, 1, and 10 ppb, respectively. The procedure was applied to determine ABZ and its major metabolites in the incurred muscle tissue of yellow perch obtained after orally dosing the fish with ABZ.

Indexes of Lipid Metabolism in Fish from the Zaporizke Reservoir

2017 ◽  
Vol 64 ◽  
pp. 10-16 ◽  
Author(s):  
Tamila Ananieva
Keyword(s):  
Muscle Tissue ◽  
Total Lipid ◽  
Iodine Value ◽  
Liver Tissue ◽  
Predatory Fish ◽  
Contamination Level ◽  
European Perch ◽  
Tissue Samples ◽  
Lipid Contents ◽  
Pike Perch

The total lipid contents and iodine value of fats had been determined in fish from two sections of the Zaporizke Reservoir (Ukraine) with different contamination levels. Research was conducted using the muscle and liver tissue samples from pike-perch (Sander lucioperca), european perch (Perca fluviatis), prussian carp (Carassius gibelio), roach (Rutilus rutilus) and rudd (Scardinius erythrophthalmus). Obtained data showed that at the contaminated zone, the total lipid contents were significantly (p ≤ 0.05) reduced in muscle tissue of pike-perch and european perch in comparison with the samples from “conventionally clean” lower section of the reservoir. Increased iodine value of fats in muscle tissue and liver tissue were detected in the predatory fish and both fish groups respectively. The research results could be used for estimation of the adaptation processes in freshwater fish as well as for indication of environmental contamination level in the natural and artificial reservoirs.

scholarly journals Safety Assessment of the Auto Manipulation Device for Acupuncture in Sprague-Dawley Rats: Preclinical Evaluation of the Prototype

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Geng-Hao Liu ◽  
Meng-Yen Tsai ◽  
Gwo-Jyh Chang ◽  
Chao-Min Wu ◽  
Sheng-Kai Lin ◽  
...  
Keyword(s):  
Real Time ◽  
Muscle Tissue ◽  
Safety Issue ◽  
Laboratory Data ◽  
Needle Insertion ◽  
Control Group ◽  
Sprague Dawley ◽  
Tissue Samples ◽  
Physiological Functions ◽  
Whole Process

Background. The Auto Manipulation Device for Acupuncture (AMDA) is designed for providing stable, quantified effects and higher frequency when doing lifting and thrusting manipulation. The purpose of this study is to investigate the safety of manipulation by AMDA in different frequency and duration in healthy rats. Methods. The study was divided into two parts: single intervention and once a day for a week. 12 rats and 15 rats were randomly allocated to different groups: Control (needle insertion only), AMDA (2Hz/10Mins), AMDA (2Hz/20Mins), AMDA (20Hz/10Mins), and AMDA (20Hz/20Mins) for single and repeated interventions. Real-time physiological functions, laboratory data, and the bilateral muscle tissue of acupoint (ST 36) were obtained after the intervention. Results. We found neither real-time physiological functions nor laboratory data differences between control group and AMDA groups in both parts. In the muscle tissue samples, the slight damage had been observed in the AMDA group with a frequency of 2 Hz for 20 minutes after once intervention, and the repeated session groups noted more obvious tissue damage with fibrotic change. Although the period was shorter, higher frequency manipulation caused more damage that fibroblast nuclei became more slender and obvious. However, no significant adverse effect was noted such as crippled and molting in the whole process. Conclusion. Our study suggested that the safety issue of AMDA operation in rats is feasible because there was no difference between control group and AMDA groups among real-time physiological functions and laboratory data. However, manipulation with higher frequency should be more preserved.

scholarly journals TRICHINELLOSIS OF THE STONE MARTEN (MARTES FOINA) IN CENTRAL RUSSIA

2021 ◽  
pp. 56-60
Author(s):  
Andreyanov
Keyword(s):  
Muscle Tissue ◽  
Diaphragm Muscle ◽  
Stone Marten ◽  
Martes Foina ◽  
Tissue Samples ◽  
Central Russia ◽  
Animal Muscle ◽  
Artificial Gastric Juice ◽  
Males And Females ◽  
Fur Animals

Over the past 5 years, research has been carried out in natural biocenosis on the stone marten (Martes foina) infected with the pathogen of trichinellosis in Central Russia. Biological material for research was collected in hunting farms, reserves and "green zones" of the Central Region of Russia (the Vladimir, Moscow, Nizhny Novgorod, Tula, Ryazan, Oryol and Bryansk regions) during the opening of amateur sports hunting for fur animals from 2017 to 2021. Diagnostics and isolation of helminth larvae from animal muscle tissue samples were performed by the compressor trichinelloscopy and digestion in artificial gastric juice. The degree of host invasion with helminth and invasion intensity were determined. A total of 35 animals were studied. As a result of studies, the infection rate of helminthozoonosis in animals was from 8.3 to 40.0%. The intensity of invasion was recorded between 2 and 39 larvae in 1 g of diaphragm muscle tissue. Trichinella invasion among martens was observed equally in both males and females. The largest percentage of the invasive form of the helminthiasis pathogen occurred in animals older than 2 years. The adverse situation of helminthozoonosis among fur animals was noted in the Ryazan region.

scholarly journals Residue of ochratoxin a in chicken tissues-risk assessment

2011 ◽  
Vol 19 (1-2) ◽  
pp. 23-27 ◽  
Author(s):  
Dragan Milicevic ◽  
Milijan Jovanovic ◽  
Vesna Matekalo-Sverak ◽  
Tatjana Radicevic ◽  
Milan Petrovic ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Extraction Procedure ◽  
Maximum Level ◽  
Liquid Extraction ◽  
Preliminary Evaluation ◽  
Retail Market ◽  
Tissue Samples ◽  
Chicken Tissues ◽  
Liquid Liquid Extraction ◽  
Agricultural Areas

Background: Toxicological investigations of tissues of normally slaughtered chickens were carried out to provide preliminary evaluation of the incidence of OTA in chicken tissues (n=90). Majority of tissue samples were not found to contain measurable amounts of OTA, while in general, the OTA levels found in the analyzed tissue were low. Methods: The presence of OTA in tissue samples was determined by HPLC-FL after liquid-liquid extraction procedure. Method validation was performed according to the Commission Decision 2002/657/EC. Results: Of the 90 liver, kidney and gizzard samples originating from chicken farms located in the different agricultural areas of Serbia, OTA was reported in 23 (38.33%), 17 (28.3%) and 16 (26.6%) samples, respectively, with levels ranging from 0.14 to 3.9 ng/g in liver, 0.1 to 7.02 ng/g in kidneys and 0.25 to 9.94 ng/g in gizzard. None of the tissue samples contained more than the maximum level (10 ng/g) recommended by the European Commission. Conclusion: Low OTA results also suggested that chicken meat available in the retail market is unlikely to pose an adverse health risk to the consumers in respect to OTA toxicity.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Sulfadimethoxine in Catfish (Ictalurus punctatus) Muscle Tissue

1990 ◽  
Vol 73 (6) ◽  
pp. 868-871 ◽  
Author(s):  
Austin R Long ◽  
Lily C Hsieh ◽  
Marsha S Malbrou ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Fish Muscle ◽  
Tissue Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method for the isolation and liquid chromatographic determination of sulfadimethoxine In catfish (Ictalurus punctatus) muscle tissue Is presented. Blank control and sulf adlmethox- Ine-fortlfled fish muscle tissue samples (0.5 g) were blended with octadecylsllyl (C18,40 /μm, 18% load, endcapped) derivatlzed silica packing material. A column made from the C18/ fish tissue blend was first washed with hexane (8 mL), following which the sulfadimethoxine was eluted with dlchloromethane (8 mL). The eluant contained sulfadimethoxine analyte that was free from Interfering compounds when analyzed by liquid chromatography with UV detection (photodlode array, 270 nm). Standard curves for sulfadimethoxine Isolated from fortified samples were linear (0.999 ± 0.001) with an average relative percentage recovery of 101.1 ± 4.2% for the concentration range (50, 100, 200,400, 800, and 1600 ng/g) examined using sulfamethoxazole as the Internal standard. The interassay variability was 10.7 ± 8.2% with an Intra-assay variability of 2.2%.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Oxytetracycline in Catfish (Ictalurus punctatus) Muscle Tissue

1990 ◽  
Vol 73 (6) ◽  
pp. 864-867 ◽  
Author(s):  
Austin R Long ◽  
Lily C Hsiesh ◽  
Marsha S Malbrough ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Fish Muscle ◽  
Butylated Hydroxytoluene ◽  
Tissue Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method for Isolation and liquid chromatographic determination of oxytetracycllne In catfish (Ictalurus punctatus) muscle tissue Is presented. Blank control and oxytetracycllne- fortlfied fish muscle tissue samples (0.5 g) were blended with octadecylsilyl (C18, 40 μm, 18% load, endcapped) derlvatized silica packing material (2 g) containing 0.05 g each of oxalic acid and disodlum ethylenedlamlnetetraacetate. A column made from the C18/fish tissue matrix was first washed with hexane (8 mL), following which the oxytetracycllne was eluted with acetonltrile-methanol (1 + 1, v/v) containing 0.06% w/v each of butylated hydroxyanlsole and butylated hydroxytoluene. The eluate contained oxytetracycllne analyte that was free from Interfering compounds when analyzed by liquid chromatography with UV detection (photodlode array set at 365 nm). Standard curves for oxytetracycllne Isolated from fortified samples were linear (0.998 ± 0.002) with an average absolute percentage recovery of 80.9 ± 6.6% for the concentration range (50,100,200, 400, 800, 1600, and 3200 ng/g) examined. The Interassay variability was 11.3 ± 5.2% with an Intra-assay variability of 1.1%.

scholarly journals Atlantic bluefin tuna (Thunnus thynnus) farmed in the Adriatic Sea

2008 ◽  
Vol 52 (No. 4) ◽  
pp. 175-177 ◽  
Author(s):  
E. Srebocan ◽  
J. Pompe-Gotal ◽  
A. Prevendar-Crnic ◽  
E. Ofner
Keyword(s):  
European Commission ◽  
Muscle Tissue ◽  
Liver Tissue ◽  
Total Mercury ◽  
Adriatic Sea ◽  
Maximum Level ◽  
Bluefin Tuna ◽  
Thunnus Thynnus ◽  
Commission Decision ◽  
Wet Weight

Levels of total mercury in the muscle (29 samples) and liver tissue (15 samples) of bluefin tuna (<i>Thunnus thynnus</i>) weighing 100&minus;300 kg were determined by cold vapour atomic absorption (AA) spectroscopy. Tunas were previously captured in the waters of Malta, towed to the farm in the Adriatic Sea and fattened with defrosted herring and sardine for the period of 6 to 7 months. The purpose of the investigation was to determine the magnitude of mercury contamination and to ascertain whether the concentrations in muscle tissue exceeded the maximum level defined by the European Commission Decision (1 &mu;g/g wet weight). Total mercury concentrations in the muscle tissue of tunas ranged from 0.49 to 1.809 (median 0.899 &mu;g/g wet weight) while in the liver tissue it was from 0.324 to 3.248 (median 1.165 &mu;g/g wet weight). Total mercury concentrations in six samples of sardine ranged from 0.050 to 0.072 &mu;g/g wet weight while two samples of herring contained 0.020 and 0.053 &mu;g/g wet weight. Twelve out of 29 (41%) muscle samples of tuna contained mercury above the maximum level defined by the European Commission Decision. It is generally believed that mercury levels in Mediterranean fish are higher than those of the other seas or oceans due to numerous deposits of mercury ores and metallic mercury in surrounding countries.

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