scholarly journals Antibacterial activity ofOcimum sanctum(Tulsi), Azadirachta indica (Neem) and Phyllanthus emblica (Amla)

2016 ◽  
Vol 11 (1) ◽  
pp. 37-41
Author(s):  
ANJALI TIWARI ◽  
ANKITA PANDEY ◽  
O.P. VERMA
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Phyllanthus Emblica

Bioenhancing Effect of Cow Urine Distillate and Pepper Extract on Antibacterial Activity of Azadirachta Indica Leaves

1970 ◽  
Vol 9 (2) ◽  
pp. 3212-3214
Author(s):  
Pramod Dhakal ◽  
Ankit a Achary ◽  
Vedamurthy Joshi
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Watery Diarrhea ◽  
Diffusion Method ◽  
E Coli ◽  
Drug Molecules ◽  
Cow Urine

Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

scholarly journals UJI SITOTOKSISITAS EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP SEL FIBROBLAS BHK 21

2017 ◽  
Vol 7 (1) ◽  
pp. 48
Author(s):  
Qurni Restiani ◽  
Mandojo Rukmo ◽  
Devi Eka Juniarti
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Azadirachta Indica ◽  
Root Canal ◽  
In Vitro Study ◽  
Cytotoxicity Test ◽  
Root Canal Irrigants ◽  
Neem Leaves ◽  
Alternative Material

Background: The leaves of neem (Azadirachta indicia) is one of herbal medicine that recommended as an alternative material of root canal irrigants. The active ingredients of neem leaves such as alkaloids, tannins, saponins and flavonoids has been proven to have antibacterial activity against E. faecalis. The ideal properties of an alternative material of root canal irrigants is not only have antibacterial activity but also is not toxic to the tissues, but the toxicity of neem leaves remains unclear until now. Objective: The aims of this study to determine the toxicity effect of neem leaves extract in specific concentration. Methods: This research was an experimental laboratory in vitro study of baby hamster kidney fibroblast (BHK-21). The neem leaves extract was made by maceration method using ethanol 96% and certain dilution performed to obtain various concentrations. Cytotoxicity test was conducted by MTT assay and the optical density was measured using ELISA reader at wavelength of 620 nm. Then, the optical density values were calculated using the formula for determining the number of survival fibroblasts after tested. Results: The percentage of survival fibroblast at concentration of 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95, and 100% in sequence were 71.3%, 64, 2%, 62%, 60.2%, 40.1%, 36.3%, 36.7%, 29%, 28.4%, 27.5%, and 25.6% . Conclusion: The extract of neem leaves (Azadirachta indica) has cytotoxic effects at concentration of 70% up to 100%.

Rapid and size-controlled biosynthesis of cytocompatible selenium nanoparticles by Azadirachta indica leaves extract for antibacterial activity

2020 ◽  
Vol 264 ◽  
pp. 127353 ◽  
Author(s):  
Nayeem A. Mulla ◽  
Sachin V. Otari ◽  
Raghvendra A. Bohara ◽  
Hemraj M. Yadav ◽  
Shivaji H. Pawar
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Selenium Nanoparticles ◽  
Size Controlled

Antioxidant and Hangover Cure Effects of Compound Prescription Containing Phyllanthus emblica and Azadirachta Indica Leaf Extract

2020 ◽  
Vol 34 (5) ◽  
pp. 229-237
Author(s):  
Su-Bin Lee ◽  
In-Hwan Joo ◽  
Jong-Min Park ◽  
Su-Hyun Han ◽  
Young-Joon Wi ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Phyllanthus Emblica

scholarly journals EVALUASI DAN IDENTIFIKASI GOLONGAN SENYAWA POTENSIAL ANTIBAKTERI PADA DAUN DAN KULIT BATANG MIMBA (Azhadirachta indica A. Juss) TERHADAP Escherichia coli

2019 ◽  
Vol 11 (2) ◽  
pp. 40-48
Author(s):  
Arif Setiawansyah ◽  
Aliefman Hakim ◽  
Dyke Gita Wirasisya
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Carboxylic Acid ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Chemical Constituents ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Neem Leaves

ABSTRACT Neem (Azadirachta indica A.Juss) is a plant that potentially developed for antibacterial agent for both the leaves and barks. The aims of this study were to compare the effectiveness of the antibacterial activity of neem leav es and stem barks extract and to identify the antibacterial compounds of the most active fractions. The extraction method was done using sonication method. Antibacterial activity was evaluated using wells solid diffusion method and TLC-Bioautography. Extract fractionation was conducted using liquid-liquid partitioning method. The chemical compounds of extracts and fractions were analyzed using TLC and GCMS. The result of sonication extraction obtained neem leaves oil (12,02%), leaves crude extract (4,3%) and stem barks crude extract (16,85%). The major chemical constituents of GCMS analysis are 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS) Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-Dyhidrobenzofuran (2,69%), alpha-D-methylglucopyranoside (4,54%), palmitic acid (2,92%), Arabino-hex-1-enitol, 1,5-Anhydro-2-deoxy-(CAS) glucal (31,69%). Phytochemical screening of neem leaves oil, leaves and barks crude extract revealed the presence of alkaloids, flavonoids, phenols, saponins, triterpenoids, steroids and sterols. Antibacterial test results showed neem leaves oil was more effective than leaves and stem barks crude extract against Escherichia coli. The n-hexane fraction showed higher antibacterial activity than ethyl acetate fraction and ethanol fraction. Phytochemical screening of n-hexane fraction showed the presence of triterpenoids, steroids, sterols and phenols.   Mimba (Azadirachta indica A.Juss) merupakan tanaman yang berpotensi dikembangkan sebagai antibakteri baik bagian daun maupun kulit batang. Penelitian ini bertujuan untuk membandingkan efektivitas antibakteri ekstrak daun dan kulit batang mimba terhadap Escherichia coli dan untuk mengidentifikasi golongan senyawa potensial antibakteri pada fraksi teraktif. Pengujian aktivitas antibakteri dilakukan dengan metode difusi padat menggunakan sumuran dan KLT-Bioautografi. Fraksinasi ekstrak dilakukan dengan metode partisi. Komponen kimia ekstrak dan fraksi dianalisis menggunakan KLT dan GCMS. Hasil ekstraksi sonikasi diperoleh minyak daun (12,02%), ekstrak kasar daun (4,3%) dan ekstrak kasar kulit batang (16,85%). Skrining fitokimia menunjukkan minyak daun, ekstrak kasar daun dan ekstrak kulit batang mimba mengandung senyawa alkaloid, flavonoid, fenolik, saponin, triterpenoid, steroid dan sterol. Komponen kimia mayor hasil analisis GCMS minyak daun mimba adalah 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS)Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-dyhidrobenzofuran (2,69%), Alpha-d-methylglucopyranoside (4,54%), Asam Palmitat (2,92%), Arabino-hex-1-enitol, 1,5-anhydro-2-deoxy-(CAS)glucal (31,69%). Hasil uji antibakteri menunjukkan minyak daun lebih efektif menghambat pertumbuhan Escherichia coli dibandingkan dengan ekstrak kasar daun dan kulit batang. Fraksi n-heksan menunjukkan aktivitas antibakteri paling besar dibandingkan dengan fraksi etil asetat dan etanol. Hasil skrining fitokimia fraksi n-heksan menunjukkan adanya senyawa triterpenoid, steroid, sterol dan fenolik.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

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