OXYGEN-DEPENDENT PHAGOCYTOSIS OF BLOOD MONOCYTES IN CHILDREN WITH HELICOBACTER PYLORI-ASSOCIATED GASTRIC AND DUODENAL EROSIONS AND ULCER

The aim of the research is to study oxygen-dependent phagocytosis of blood monocytes in children with gastric and duodenal erosions and ulcers by chemiluminescence analysis. The subjects of the research were blood monocytes, extracted from blood in 44 children with gastric and duodenal erosions and ulcers in the ages from 11 to 18 years. Microscopic tests for the bioptats of gastric mucosa of both standard regions and edges of ulcer defects had resulted in the determination of 2 groups of the patients with gastric and duodenal erosions and ulcers. The 1st group was represented by Helicobacter pylori high dissemination. As for the 2nd group, the patients showed low bacterization. The tests for luminol-dependent hemiluminescence of blood monocytes in patients with H. pylorihigh dissemination of gastric and duodenal mucosa demonstrated the significant increase of the intensity and the growth of areas both under the curve of spontaneous response and under the curve in the zimozan-induced process as compared to the monocyte activity in the group with low dissemination. Following the lucigenin- dependent chemiluminescence reactions in the group with H. pylori high dissemination we had found significant increase of the time of approaching the peak in both spontaneous response and zimozan- induced processes while the activation index was higher in comparison with phagocyte activities of monocytes in the group with low dissemination. Further stage of the research was to identify CagA-positive strains of H. pylori in the children with gastric and duodenal erosions and ulcers. Studying chemiluminescence activity of blood lymphocytes in the patients with anti-CagA antibodies we found the true increase of the time of reaching the peak, the intensity and the area under the curve in spontaneous process in luminol-dependent response and the time of reaching intensity peak and the intensity of spontaneous chemiluminescence reaction, lucigenin being an activator. So we marked the increase of the activity of oxygen-dependent phagocytosis of blood monocytes in children with H. pylori associated with gastric and duodenal erosions and ulcers related to H. pylori increased bacterization. The growth of H. pylori dissemination results in the higher stage of stomach mucosa inflammation. Therefore active phagocytes generate more intensively the formation of active forms of oxygen, free radicals and the products of peroxide oxidation. CagA-positive strains of H. pylori, as a rule, are associated with the higher level of inflammatory activity than CagA-negative ones. As a result of such influence the functional activity of monocytes increases, because they are «professional » phagocytes. The ability to perform phagocytosis is better expressed in them as compared to other leukocytes.

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Helicobacter pylori in the population of the developmental age

Progress in Health Sciences ◽

10.5604/01.3001.0009.5138 ◽

2016 ◽

Vol 6 (1) ◽

pp. 0-0

Author(s):

E. Maciorkowska ◽

A. Gładka ◽

I. Roszko-Kirpsza

Keyword(s):

Helicobacter Pylori ◽

Oral Cavity ◽

Human Population ◽

Bacterial Infections ◽

Natural Habitat ◽

Duodenal Mucosa ◽

Developmental Age ◽

The Family ◽

H Pylori

Helicobacter pylori – Gram-negative rod, discovered more than 30 years ago, has a proven influence on inflammation of gastric and duodenal mucosa. The worldwide prevalence of H. pylori infection in the human population is estimated at 50% and is considered to be one of the most frequent bacterial infections in people. Many studies suggest that infection takes place in the early childhood within the family. The gastric mucosa is its natural habitat. In last time more and more findings about existence of this bacteria in another places of gastrointestinal tract and correlation with many diseases, especially an inflammation of oral cavity. The percentage of H. pylori detectability in the oral cavity ranges from 0 to 100%. Thus, more studies aimed at final determination of the bacterium reservoir in the oral cavity seem to be necessary.

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Implication of the Structure of the Helicobacter pylori cag Pathogenicity Island in Induction of Interleukin-8 Secretion

Infection and Immunity ◽

10.1128/iai.69.3.1625-1629.2001 ◽

2001 ◽

Vol 69 (3) ◽

pp. 1625-1629 ◽

Cited By ~ 40

Author(s):

C. Audibert ◽

C. Burucoa ◽

B. Janvier ◽

J. L. Fauchère

Keyword(s):

Helicobacter Pylori ◽

Clinical Presentation ◽

Blot Hybridization ◽

Pathogenicity Island ◽

Interleukin 8 ◽

Middle Region ◽

Dot Blot ◽

Long Distance ◽

H Pylori

ABSTRACT Helicobacter pylori virulence is associated with the presence of the cag pathogenicity island (PAI). Thecag PAI is involved in the ability to induce interleukin-8 (IL-8) secretion by human cells, which is implicated in the inflammatory response of the gastric mucosa to H. pyloriinfection. The aim of this study was to determine whether the genetic structure of the cag PAI is conserved and whether it is linked to IL-8 induction ability. Detection of specific markers (cagA, picB, cag13-cag14, virD4, and IS605) by PCR and dot blot hybridization and long-distance PCR determination of the presence of cagI, cagII, and the middle region of thecag PAI were performed on 153 strains isolated from adults suffering from ulcers (n = 79) or gastritis (n = 74). IL-8 induction ability was evaluated by coculture of the strains with HEp-2 cells. Eighty-three strains (54.3%) had an entire cag PAI, 12 strains (7.8%) had thecag PAI split in two, 49 strains (32%) had nocag PAI, and 9 strains exhibited other structural combinations. The presence of an entire cag PAI was statistically correlated with the presence of IS605(P = 0.006) and the ability to induce IL-8 secretion but not with clinical presentation of the infection. The structure of the cag PAI appears to be rather conserved and is related to the proinflammatory power of a strain. The existence of strains inducing IL-8 secretion regardless of the cag PAI structure suggests that this region is not the only requirement for IL-8 secretion.

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Mucosal FOXP3-Expressing CD4+ CD25high Regulatory T Cells in Helicobacter pylori-Infected Patients

Infection and Immunity ◽

10.1128/iai.73.1.523-531.2005 ◽

2005 ◽

Vol 73 (1) ◽

pp. 523-531 ◽

Cited By ~ 186

Author(s):

Anna Lundgren ◽

Erika Strömberg ◽

Åsa Sjöling ◽

Catharina Lindholm ◽

Karin Enarsson ◽

...

Keyword(s):

T Cells ◽

Helicobacter Pylori ◽

Regulatory T Cells ◽

Gastric Adenocarcinoma ◽

Cytotoxic T Lymphocyte ◽

Duodenal Mucosa ◽

Peptic Ulcers ◽

Low Levels ◽

H Pylori ◽

And Function

ABSTRACT Helicobacter pylori chronically colonizes the stomach and duodenum and causes peptic ulcers or gastric adenocarcinoma in 10 to 20% of infected individuals. We hypothesize that the inability of patients to clear H. pylori infections is a consequence of active suppression of the immune response. Here we show that H. pylori-infected individuals have increased frequencies of CD4+ CD25high T cells in both the stomach and duodenal mucosa compared to uninfected controls. These cells have the phenotype of regulatory T cells, as they express FOXP3, a key gene for the development and function of regulatory T cells, as well as high levels of the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) protein. In contrast, mucosal CD4+ CD25low and CD4+ CD25− cells express little FOXP3 mRNA and low levels of the CTLA-4 protein. Mucosal CD4+ CD25high T cells are present in individuals with asymptomatic H. pylori infections as well as in duodenal ulcer patients. The frequencies of CD4+ CD25high cells are also increased in the stomachs of H. pylori-infected patients with gastric adenocarcinoma, particularly in cancer-affected tissues. These findings suggest that regulatory T cells may suppress mucosal immune responses and thereby contribute to the persistence of H. pylori infections.

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ASSOCIATION OF DUODENAL EOSINOPHILIC INFILTRATE WITH HELICOBACTER PYLORI INFECTION, BUT NOT WITH FUNCTIONAL DYSPEPSIA

Arquivos de Gastroenterologia ◽

10.1590/s0004-2803.202000000-13 ◽

2020 ◽

Vol 57 (1) ◽

pp. 74-78 ◽

Cited By ~ 1

Author(s):

Carine LEITE ◽

Luiz Edmundo MAZZOLENI ◽

Diego de Mendonça UCHOA ◽

Juliana Araújo CASTANHO ◽

Felipe MAZZOLENI ◽

...

Keyword(s):

Helicobacter Pylori ◽

Functional Dyspepsia ◽

Eosinophil Count ◽

Gastrointestinal Symptoms ◽

Duodenal Mucosa ◽

Helicobacter Pylori Infection ◽

Eosinophilic Infiltrate ◽

Urease Test ◽

H Pylori

ABSTRACT BACKGROUND: The role of Helicobacter pylori infection on eosinophilic infiltration in duodenal mucosa is poorly studied. An increase in the number of eosinophils in duodenum has been associated with functional dyspepsia. OBJECTIVE: To evaluate the influence of H. pylori infection on duodenal eosinophil count and the role of eosinophilic infiltrate of duodenum in functional dyspepsia. METHODS: Positive and negative H. pylori individuals were included. Both functional dyspeptic patients according to Rome III criteria (cases) and individuals without gastrointestinal symptoms (controls) were enrolled. They were submitted to upper endoscopy and H. pylori infection was verified by gastric histopathology and urease test. Eosinophils in the duodenal mucosa were counted in five high-power fields, randomly selected on slides of endoscopic biopsies. RESULTS: Thirty-nine H. pylori positive (mean age 40.5 and 69.2% women) and 24 negative patients (mean age 37.3 and 75% women) were included. The influence of the infection was observed in the duodenal eosinophil count, which was higher in infected individuals: median 13.2 vs 8.1 in non-infected individuals (P=0.005). When we analyzed patients according to symptoms, cases - mean age 39.6; 71.4% women - and controls - mean age 38.7; 71.4% women - had similar duodenal eosinophil count: median 11.9 and 12.6 respectively (P=0.19). CONCLUSIONS: We did not demonstrate association of duodenal eosinophil count with functional dyspepsia but found association with H. pylori infection.

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HELICOBACTER PYLORI INFECTION;

The Professional Medical Journal ◽

10.29309/tpmj/2017.24.09.884 ◽

2017 ◽

Vol 24 (09) ◽

pp. 1425-1430

Author(s):

Tahira Tabassum ◽

Ayesha Imtiaz ◽

Aamir Sharif ◽

Muhammad Akram

Keyword(s):

Helicobacter Pylori ◽

Age Groups ◽

Helicobacter Pylori Infection ◽

Step Test ◽

Chromatographic Technique ◽

Medical College ◽

Education Levels ◽

One Step ◽

H Pylori

Objectives: To estimate the prevalence of Helicobacter pylori infection inpopulation of Sargodha and determination of possible risk factors. Design: Descriptive study.Place and Duration: University medical complex & Research Center, Sargodha Medical College,Sargodha from 01.01.2017 to 31.03.2017. Material and Methods: The study was conductedamong 486 clinically suspected individuals of Sargodha, Punjab, Pakistan. Data was obtainedby questionnaire and H. pylori antibodies were detected by H. pylori ‘One Step Test Device’based on immune-chromatographic technique. Results: A total of 486 individuals participatedin the study of which 327 (67.28%) were positive for H. pylori infection. The prevalence was66.97% in males and 33.2% in females (p = 0.00) and increased with increasing age (p =0.000). In the study, significant association was found between age groups, education levels,food habits, monthly income with H. pylori infection with p values 0.000, 0.000, 0.001, 0.041respectively while no significant association was seen for source of drinking water (p =0.321).Conclusion: The results of present study showed the prevalence of H. pylori in reported areaand confirmed that it is higher in developing countries. It is concluded that prevalence ofinfection with this bacterium can be minimized by increasing the hygenicity, improving the livingstandards and, of course, by educating the nation.

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Effect of sequential eradication therapy on serum osteoprotegerin levels in patients with Helicobacter pylori infection and co-existing inflammatory bowel disease

Journal of International Medical Research ◽

10.1177/03000605211060648 ◽

2021 ◽

Vol 49 (11) ◽

pp. 030006052110606

Author(s):

Hussam Murad ◽

Misbahuddin Rafeeq ◽

Mahmoud Mosli ◽

Mamdouh Gari ◽

Mohammed Basheikh

Keyword(s):

Inflammatory Bowel Disease ◽

Helicobacter Pylori ◽

Bowel Disease ◽

Eradication Therapy ◽

Area Under The Curve ◽

Control Group ◽

Characteristic Analysis ◽

Cross Sectional ◽

Inflammatory Bowel ◽

H Pylori

Objective To investigate the effect of sequential Helicobacter pylori eradication therapy on serum osteoprotegerin levels in patients with H. pylori infection and co-existing inflammatory bowel disease (IBD). Methods Three groups of patients were involved in this observational cross-sectional study: IBD (n = 83), H. pylori infection (HP, n = 68), and H. pylori infection with co-existing IBD (HP + IBD, n = 52). These groups were compared with a normal control group (NC, n = 50). Serum osteoprotegerin, serum bone alkaline phosphatase (BALP), and fecal calprotectin (FC) levels were measured. Results Serum osteoprotegerin levels were significantly correlated with the simple endoscopic score for Crohn’s disease and Mayo score for ulcerative colitis. The receiver operating characteristic analysis of osteoprotegerin revealed high values for the area under the curve, sensitivity, and specificity. Discriminant analysis illustrated that osteoprotegerin levels significantly differentiated patients with IBD from healthy controls. Osteoprotegerin and FC levels distinguished the IBD and HP + IBD groups from the NC and HP groups. Conclusions Sequential eradication therapy did not affect serum osteoprotegerin levels in patients with H. pylori infection and co-existing IBD. Serum osteoprotegerin elevation might be a marker for IBD development in patients with past or current H. pylori infection.

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Micro-broth dilution method with air-dried microplate for determining MICs of clarithromycin and amoxycillin for Helicobacter pylori isolates

Journal of Medical Microbiology ◽

10.1099/jmm.0.05397-0 ◽

2004 ◽

Vol 53 (5) ◽

pp. 403-406 ◽

Cited By ~ 8

Author(s):

Intetsu Kobayashi ◽

Hiroe Muraoka ◽

Takeshi Saika ◽

Minoru Nishida ◽

Toshio Fujioka ◽

...

Keyword(s):

Helicobacter Pylori ◽

Close Agreement ◽

Reliable Method ◽

Agar Plate ◽

Dilution Method ◽

Air Drying ◽

H Pylori ◽

Broth Dilution

MICs of clarithromycin and amoxycillin for 253 isolates of Helicobacter pylori were measured by an air-dried microplate method and compared with the results obtained by the agar plate dilution method. The air-dried microplate method is performed by coating each well of a 96-well microplate with the test antibiotic and air-drying it. There were no marked differences between the air-dried microplate method and agar plate dilution methods in the MIC50 and MIC90 values or MIC ranges of clarithromycin obtained for the 253 isolates of H. pylori. More specifically, the MICs of clarithromycin for 114 (45.1 %) of the 253 isolates were the same by the air-dried microplate method as the agar plate dilution method, and the differences in the MICs of clarithromycin for a further 114 isolates (45.1 %) varied within one twofold dilution. The MICs of amoxycillin by the former method were in close agreement with the MICs obtained by the latter method: MICs of amoxycillin for 199 (78.7 %) of the 253 isolates were the same by both methods, and the differences in the MICs of amoxycillin for 42 isolates (16.6 %) varied within one twofold dilution. These results indicate that the air-dried microplate method is a useful method for determination of MICs, because the results obtained were in close agreement with those obtained by the standard agar plate dilution method. The air-dried microplate method is, therefore, a convenient and reliable method for determining the MICs of clarithromycin and amoxycillin for H. pylori isolates.

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Molecular Detection of Antibiotic Resistance in South African Isolates ofHelicobacter pylori

Gastroenterology Research and Practice ◽

10.1155/2013/259457 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 19

Author(s):

Nicoline F. Tanih ◽

Roland N. Ndip

Keyword(s):

Helicobacter Pylori ◽

Antibiotic Resistance ◽

South African ◽

Molecular Detection ◽

Rapid Diagnosis ◽

Biopsy Specimens ◽

H Pylori ◽

Detection Of Mutations ◽

Reverse Hybridisation Assay

Rapid diagnosis and treatment ofHelicobacter pylori(H. pylori) presents a challenge. We aimed at investigating the presence ofH. pylori, susceptibility profile, and associated mutations in an effort to validate the effectiveness of GenoType HelicoDR assay inH. pylorityping in our environment. Two hundred and fifty-four biopsy specimens were cultured and DNA extracted from seventy-eight positive cultures using the Qiagen DNA extraction kit. The GenoType Helico DR which employs reverse hybridisation was used to confirm the presence ofH. pylori, determination of its susceptibility to antimicrobials, and detection of mutations conferring resistance to clarithromycin and fluoroquinolones. The organism was isolated from 168/254 (66.1 %) of the specimens by culture. Of the 78 strains used for further investigation, 12/78 (15.38%) were resistant to clarithromycin while 66/78 (84.61%) were susceptible. For fluoroquinolone, 70/78 (89.74%) strains were susceptible while 8 (10.26%) were resistant. Mutations were observed in 17 strains with A2147G being the most prevalent; A2146C and D91N were the least. The reverse hybridisation assay is an easy and fast technique in confirming the presence ofH. pylori, its antimicrobial profile, and associated mutations. Analysis regarding the suitability of this assay forH. pylorityping is warranted in other regions.

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THE PREVALENCE OF CAGA HELICOBACTER PYLORI AND CHARACTERISTICS OF AN ASSOCIATED GASTRITIS IN SCHOOLCHILDREN IN THE REPUBLIC OF TYVA

Российский педиатрический журнал ◽

10.18821/1560-9561-2017-20-1-19-23 ◽

2019 ◽

Vol 20 (1) ◽

pp. 19-23

Author(s):

T. V. Polivanova ◽

V. A. Vshivkov

Keyword(s):

Helicobacter Pylori ◽

The Body ◽

Adverse Impact ◽

Cross Sectional ◽

Antral Gastritis ◽

Ethnic Populations ◽

Cytotoxin Associated Gene A ◽

The Republic ◽

H Pylori

Aim of the research. To study the prevalence of H. pylori cytotoxin-associated gene A (CagA) strain and estimate the activity of associated gastritis in schoolchildren with dyspepsia syndrome in the Republic of Tyva. Materials and Methods. We studied the prevalence of H. pylоri CagA strain by performing the cross-sectional examination for picked up randomly 1064 schoolchildren aged of from 7 to 17 years, residing in the Republic of Tyva. We used the technique of determination of blood IgG to CagA antigen of H. pylori in 218 children, including 131 cases with dyspeptic complaints. We also executed esophagogastroduodenoscopy including biopsy sampling. Results. CagA-seropositive children accounted for 47.2%. We noted the increase in the activity of antral gastritis in H. pylori-infected schoolchildren with the maximum being related to CagA-seropositive schoolchildren. Gastritis activity in the body of stomach in H. pylori-infected children was higher as compared to non-infected children, but as a whole it was lower than in antral region of the stomach. Besides that we revealed specific features of gastritis activities in the body and in the antral region of stomach in children in ethnic populations. The peculiarities are referred to the presence of the differences between the mentioned parameters in alien schoolchildren and the absence of such differences in the Tyvan schoolchildren. Conclusion. Widely spread H. pylori, in particular CagA strain of the microorganism, plays the adverse role in the development and especially progressing of gastritis in schoolchildren residing in the Republic of Tyva. The importance of the adverse impact of the infection is associated with the ethnicity of children.

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Diagnosis of Helicobacter pylori infection and determination of clarithromycin resistance by fluorescence in situ hybridization from formalin-fixed, paraffin-embedded gastric biopsy specimens

Canadian Journal of Microbiology ◽

10.1139/w05-035 ◽

2005 ◽

Vol 51 (7) ◽

pp. 569-573 ◽

Cited By ~ 17

Author(s):

Fusun Can ◽

Zerrin Yilmaz ◽

Muge Demirbilek ◽

Banu Bilezikci ◽

Ganiye Kunefeci ◽

...

Keyword(s):

Helicobacter Pylori ◽

Gastric Biopsy ◽

Test Method ◽

Biopsy Specimens ◽

Clarithromycin Resistance ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

H Pylori ◽

Formalin Fixed

A reliable diagnostic test for Helicobacter pylori is important in clinical practice and research. The ideal diagnostic test for H. pylori should be sensitive, specific, and cost-effective. Helicobacter pylori resistance to clarithromycin is a common reason for failure of eradication therapy. The aim of this study was to evaluate the fluorescent in situ hybridization (FISH) method to detect H. pylori and determine clarithromycin resistance in formalin-fixed, paraffin-embedded gastric biopsy specimens. One hundred seventeen gastric biopsy specimens from patients with dyspepsia were examined for the presence of H. pylori by conventional culture, FISH, and histopathological methods. A set of fluorescent-labeled oligonucleotide probes binding to either H. pylori 16S rRNA or 23S rRNA sequences were used for FISH analysis. Phenotypic antibiotic susceptibilities of the isolates were tested using the Epsilometer test method (E test). Helicobacter pylori was detected in 70 of 117 biopsy specimens by histopathological examination and FISH, whereas it was detected in 47 specimens by culturing. Histopathology and FISH techniques failed to identify H. pylori in 1 biopsy sample isolated by culture. Clarithromycin resistance was found in 11 of 46 H. pylori isolates using the E test method. All of the phenotypic resistance measurements of isolates were correlated with genotypic clarithromycin resistance. Eleven clarithromycin-resistant strains were identified by FISH. The diagnosis of H. pylori infection and the determination of clarithromycin resistance in formalin-fixed, paraffin-embedded specimens using FISH is promising because it provides a rapid, reliable, and culture-independent diagnosis.Key words: Helicobacter pylori, clarithromycin resistance, FISH.

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