Helicobacter pylori in the population of the developmental age

2016 ◽  
Vol 6 (1) ◽  
pp. 0-0
Author(s):  
E. Maciorkowska ◽  
A. Gładka ◽  
I. Roszko-Kirpsza
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Human Population ◽  
Bacterial Infections ◽  
Natural Habitat ◽  
Duodenal Mucosa ◽  
Developmental Age ◽  
The Family ◽  
H Pylori

Helicobacter pylori – Gram-negative rod, discovered more than 30 years ago, has a proven influence on inflammation of gastric and duodenal mucosa. The worldwide prevalence of H. pylori infection in the human population is estimated at 50% and is considered to be one of the most frequent bacterial infections in people. Many studies suggest that infection takes place in the early childhood within the family. The gastric mucosa is its natural habitat. In last time more and more findings about existence of this bacteria in another places of gastrointestinal tract and correlation with many diseases, especially an inflammation of oral cavity. The percentage of H. pylori detectability in the oral cavity ranges from 0 to 100%. Thus, more studies aimed at final determination of the bacterium reservoir in the oral cavity seem to be necessary.

scholarly journals OXYGEN-DEPENDENT PHAGOCYTOSIS OF BLOOD MONOCYTES IN CHILDREN WITH HELICOBACTER PYLORI-ASSOCIATED GASTRIC AND DUODENAL EROSIONS AND ULCER

2018 ◽  
Vol 8 (2) ◽  
pp. 157-163 ◽  
Author(s):  
O. A. Kolenchukova ◽  
I. N. Gvozdev ◽  
N. N. Gorbachova ◽  
I. S. Litvinova
Keyword(s):  
Helicobacter Pylori ◽  
Oxygen Free Radicals ◽  
Area Under The Curve ◽  
Duodenal Mucosa ◽  
Blood Monocytes ◽  
Peroxide Oxidation ◽  
Active Forms Of Oxygen ◽  
Spontaneous Chemiluminescence ◽  
H Pylori

The aim of the research is to study oxygen-dependent phagocytosis of blood monocytes in children with gastric and duodenal erosions  and ulcers by chemiluminescence analysis. The subjects of the  research were blood monocytes, extracted from blood in 44 children  with gastric and duodenal erosions and ulcers in the ages from 11 to  18 years. Microscopic tests for the bioptats of gastric mucosa of  both standard regions and edges of ulcer defects had resulted in the  determination of 2 groups of the patients with gastric and duodenal  erosions and ulcers. The 1st group was represented by Helicobacter  pylori high dissemination. As for the 2nd group, the patients showed  low bacterization. The tests for luminol-dependent hemiluminescence of blood monocytes in patients with H. pylorihigh  dissemination of gastric and duodenal mucosa demonstrated the  significant increase of the intensity and the growth of areas both  under the curve of spontaneous response and under the curve in the  zimozan-induced process as compared to the monocyte activity  in the group with low dissemination. Following the lucigenin- dependent chemiluminescence reactions in the group with H. pylori  high dissemination we had found significant increase of the time of  approaching the peak in both spontaneous response and zimozan- induced processes while the activation index was higher in  comparison with phagocyte activities of monocytes in the group with  low dissemination. Further stage of the research was to identify CagA-positive strains of H. pylori in the children with gastric and  duodenal erosions and ulcers. Studying chemiluminescence activity  of blood lymphocytes in the patients with anti-CagA antibodies we found the true increase of the time of reaching the peak, the  intensity and the area under the curve in spontaneous process in  luminol-dependent response and the time of reaching intensity peak  and the intensity of spontaneous chemiluminescence reaction,  lucigenin being an activator. So we marked the increase of the  activity of oxygen-dependent phagocytosis of blood monocytes in  children with H. pylori associated with gastric and duodenal erosions  and ulcers related to H. pylori increased bacterization. The growth of  H. pylori dissemination results in the higher stage of stomach  mucosa inflammation. Therefore active phagocytes generate more  intensively the formation of active forms of oxygen, free radicals and the products of peroxide oxidation. CagA-positive strains of H. pylori, as a rule, are associated with the higher level of inflammatory  activity than  CagA-negative ones. As a result of such influence the  functional activity of monocytes increases, because they are  «professional » phagocytes. The ability to perform phagocytosis is better expressed in them as compared to other leukocytes.

scholarly journals Alteration of circulating insulin-like growth factors in patients infected with bacteria helicobacter pylori or francisella tularensis

2002 ◽  
Vol 21 (4) ◽  
pp. 339-343
Author(s):  
Ivona Baricevic ◽  
Olgica Nedic ◽  
Anna Nikolic-Judith ◽  
Slavica Marjanovic ◽  
Elizabeta Ristanovic ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Growth Factors ◽  
Francisella Tularensis ◽  
Bacterial Infections ◽  
Bacterial Species ◽  
Igf System ◽  
Igf I ◽  
H Pylori ◽  
Insulin Like Growth Factors

Alterations of insulin-like growth factors (IGF-I and -II) and their binding proteins (IGFBP) in patients infected with Helicobacter pylori or Francisella tularensis are reported in this paper. Infections were diagnosed immunochemically, by determination of specific antibodies to each bacterial species. It was shown that IGF-I, IGF-II and IGFBP-3 concentrations were lower in patients with bacterial infections, while IGFBP-2 concentration increased in comparison with healthy adults. Although the effect was more pronounced in the case of H. pylori infection, statistically significant reductions of IGF-I and IGF-II were found in both groups of patients. For IGF-I p < 0.0001 and for IGF-II p = 0.037 in patients with H. pylori, while p = 0.017 and p = 0.032 in patients with F. tularensis. Alterations of the IGF system can be regarded as a combined effect of bacterial infection on immuno, gastrointestinal hepatobiliary and nutritional axes in the organism.

Determination of the prevalence of helicobacter pylori oral infection in smoking patients with chronic generalized periodontitis on the background of chronic hyperacidal gastritis during treatment

2020 ◽  
Vol 40 (4) ◽  
pp. 50-54
Author(s):  
O. L. Zolotukhina ◽  
◽  
Ju. G. Romanova ◽  
O. V. Maslov ◽  
◽  
...  
Keyword(s):  
Risk Factors ◽  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Urease Activity ◽  
Rapid Test ◽  
Oral Infection ◽  
Helicobacter Pylori Infection ◽  
Natural Origin ◽  
Periodontal Tissues

Diseases of periodontal tissues occupy one of the leading positions among modern dental problems, namely the multifactorial nature of these diseases. In modern dental science, the issue of the development of periodontal pathology against the background of somatic pathology and risk factors remains relevant. Pathology of periodontal tissues in 68–90 % of cases is accompanied by chronic diseases of the gastrointestinal tract. Today, there is no doubt that Helicobacter pylori infection can be present in the biotopes of the oral cavity and can affect the course of periodontal pathology. As you know, smoking is one of the important risk factors for the development of inflammatory-dystrophic diseases of periodontal tissues, which can aggravate the course of the latter. The purpose of the work is to determine the prevalence of oral Helicobacter pylori infection in tobacco-dependent patients with chronic generalized periodontitis on the background of chronic hyperacid gastritis during treatment. Patients who received the proposed therapeutic and prophylactic complex (ultraphonophoresis procedures with the created gel «Apisan», and probiotic drug BioGaia ProDentis and angioprotective drug of natural origin — Detralex) showed a gradual decrease in the level of total urease activity and, as a consequence, a decrease the prevalence of Helicobacter pylori infection in the oral cavity according to the results of a urease rapid test with material from the oral cavity, both in the presence of a risk factor — smoking, and in its absence. The use of the proposed therapeutic and prophylactic complex proved to be effective in reducing the prevalence of oral Helicobacter pylori infection in smoking patients and patients who do not smoke, with chronic generalized periodontitis against the background of chronic hyperacidal gastritis associated with Helicobacter pylori.

Synthesis and In Vitro Enzymatic Studies of New 3-Aryldiazenyl Indoles as Promising Helicobacter pylori IMPDH Inhibitors

2019 ◽  
Vol 19 (5) ◽  
pp. 376-382 ◽  
Author(s):  
Sachin Jangra ◽  
Gayathri Purushothaman ◽  
Kapil Juvale ◽  
Srimadhavi Ravi ◽  
Aishwarya Menon ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Bacterial Infections ◽  
Immunosuppressive Drugs ◽  
Multi Drug Resistance ◽  
Metabolic Enzyme ◽  
World Population ◽  
Inhibitor Molecule ◽  
Nucleotide Synthesis ◽  
H Pylori

Background & Objective:Helicobacter pylori infection is one of the primary causes of peptic ulcer followed by gastric cancer in the world population. Due to increased occurrences of multi-drug resistance to the currently available antibiotics, there is an urgent need for a new class of drugs against H. pylori. Inosine 5′-monophosphate dehydrogenase (IMPDH), a metabolic enzyme plays a significant role in cell proliferation and cell growth. It catalyses guanine nucleotide synthesis. IMPDH enzyme has been exploited as a target for antiviral, anticancer and immunosuppressive drugs. Recently, bacterial IMPDH has been studied as a potential target for treating bacterial infections. Differences in the structural and kinetic parameters of the eukaryotic and prokaryotic IMPDH make it possible to target bacterial enzyme selectively.Methods:In the current work, we have synthesised and studied the effect of substituted 3-aryldiazenyl indoles on Helicobacter pylori IMPDH (HpIMPDH) activity. The synthesised molecules were examined for their inhibitory potential against recombinant HpIMPDH.Results:In this study, compounds 1 and 2 were found to be the most potent inhibitors amongst the database with IC50 of 0.8 ± 0.02µM and 1 ± 0.03 µM, respectively.Conclusion:When compared to the most potent known HpIMPDH inhibitor molecule C91, 1 was only four-fold less potent and can be a good lead for further development of selective and potent inhibitors of HpIMPDH.

scholarly journals Helicobacter pylori colonization and oral health in children

2017 ◽  
Vol 12 (2) ◽  
pp. 41-44
Author(s):  
Vasile Valeriu LUPU ◽  
◽  
Gabriela PĂDURARU ◽  
Anca ADAM ◽  
Ana-Maria DĂBULEANU ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Health ◽  
Gastric Mucosa ◽  
Oral Cavity ◽  
Gold Standard ◽  
Dental Plaque ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Standard Triple Therapy ◽  
H Pylori

Helicobacter pylori (H. pylori) is a microaerophilic gram-negative bacterium infecting approximately one half of the world’s population. The oral cavity and dental plaque may be a reservoir for H. pylori infection. Diagnosis of H. pylori infection in children differs from that of adults. Although H. pylori has long been known to be detected in the oral cavity, the significance of such findings are controversial. Oral H. pylori may play an important role in re-infection of the gastric mucosa. The gold standard for eradicating H. pylori infection is standard triple therapy. The studies have shown promising results in the management of both oral and gastric H. pylori.

scholarly journals Prevalence ofHelicobacter pylori vacAGenotypes andcagAGene in Dental Plaque of Asymptomatic Mexican Children

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Alejandra Mendoza-Cantú ◽  
Víctor Hugo Urrutia-Baca ◽  
Cynthia Sofía Urbina-Ríos ◽  
Myriam Angélica De la Garza-Ramos ◽  
Martha Elena García-Martínez ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Dental Plaque ◽  
Gastrointestinal Disease ◽  
Oral Infection ◽  
Rrna Gene ◽  
Mexican Children ◽  
Asymptomatic Children ◽  
Dyspeptic Symptoms ◽  
H Pylori

The variability inHelicobacter pylori vacAandcagAgenes has been related to the progression of the gastrointestinal disease; also the presence ofH. pyloriin the oral cavity has been associated with periodontal disease in adults, but, in children without dyspeptic symptoms, little is known about this. We evaluated the prevalence ofH. pyloriand the presence ofvacA/cagAgenotypes in the oral cavity of Mexican children without dyspeptic symptoms. The gingival status was measured, and dental plaque samples (n=100) were taken. 38% of children were positive forH. pylori16S rRNA gene by qPCR. A significant association betweenH. pylorioral infection and gingival status was observed (P<0.001). In 34.6% (9/26) of mild gingivitis cases,s1m2genotype was found, whiles1m1was typed in 50% (3/6) of moderate gingivitis. ThecagAprevalence amongH. pylori-positive children was 80.8% (21/26), 83.3% (5/6), and 16.7% (1/6) of cases of mild gingivitis, moderate gingivitis, and nongingivitis, respectively (P<0.001). Thes1m1/cagA+ combinational genotype was the most detected in children with gingivitis. Our results suggest that the prevalence ofH. pyloriand detection ofvacA/cagAgenotypes-associated gastrointestinal disease in the oral cavity could be related to the progression of gingivitis in asymptomatic children.

scholarly journals Association between Helicobacter pylori infection and dental pulp reservoirs in Japanese adults

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Komei Iwai ◽  
Isao Watanabe ◽  
Toshiro Yamamoto ◽  
Nagato Kuriyama ◽  
Daisuke Matsui ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Dental Caries ◽  
Oral Cavity ◽  
Dental Pulp ◽  
Clinical Signs ◽  
Antibody Test ◽  
Pcr Analysis ◽  
Supragingival Plaque ◽  
Japanese Adults ◽  
H Pylori

Abstract Background Helicobacter pylori (H. pylori) colonize the stomach and are considered an etiological agent of gastric cancer. The oral cavity is a transmission route to the stomach, but the exact site of colonization has not yet been explicated. Our study investigated the association between H. pylori infection and presence in oral samples. Methods Dental pulp, supragingival plaque, and saliva from 192 patients visiting the Dentistry’s outpatient clinic were collected for testing. The H. pylori ureA gene was identified via Nested PCR. Urine anti-H. pylori antibody test was utilized to detect infection. Results Twenty-five subjects were found to be antibody-positive. PCR analysis of dental pulp revealed that 23 subjects possessed the ureA gene. Twenty-one subjects were positive for both antibodies and genes in dental pulp. PCR testing revealed that 2 subjects were positive in dental plaque but negative for saliva. The subjects positive for H. pylori in dental pulp expressed clinical signs of severe dental caries. Conclusions H. pylori infected subjects expressed H. pylori in samples from the oral cavity. The main reservoir for infection within the oral cavity was determined to be dental pulp. Moreover, H. pylori are likely transmitted from dental caries to the root canal.

The influence of Helicobacter pylori eradication therapy on the presence of H. pylori antigens in dental plaque and saliva

2016 ◽  
Vol 6 (1) ◽  
pp. 19-24
Author(s):  
DB. Namiot ◽  
K. Leszczyńska ◽  
A. Namiot ◽  
A. Kemona ◽  
R. Bucki ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Dental Plaque ◽  
Eradication Therapy ◽  
Oral Infection ◽  
Helicobacter Pylori Eradication ◽  
Immunological Method ◽  
H Pylori ◽  
Helicobacter Pylori Eradication Therapy

Purpose: The aim of this study was to evaluate the presence of H. pylori antigens in the oral cavity (dental plaque and saliva) of patients undergoing systemic eradication therapy. Materials and methods: The study was conducted in 49 subjects with H. pylori stomach infection. H. pylori antigens in dental plaque and saliva were evaluated with immunological method. Results: In subjects with initial H. pylori oral infection, the presence of H. pylori antigens in the oral cavity 6 weeks after successful or unsuccessful H. pylori eradication therapy in the stomach was 47.0% and 50.0%, respectively. In subjects without initial oral infection with H. pylori, the presence of H. pylori antigens in the oral cavity 6 weeks after successful and unsuccessful eradication therapy in the stomach was 30.0% and 20.0%, respectively. Conclusions: The immunological method detecting H. pylori antigens in the dental plaque and saliva cannot be recommended to evaluate the efficacy of H. pylori eradication in the oral cavity.

scholarly journals Implication of the Structure of the Helicobacter pylori cag Pathogenicity Island in Induction of Interleukin-8 Secretion

2001 ◽  
Vol 69 (3) ◽  
pp. 1625-1629 ◽  
Author(s):  
C. Audibert ◽  
C. Burucoa ◽  
B. Janvier ◽  
J. L. Fauchère
Keyword(s):  
Helicobacter Pylori ◽  
Clinical Presentation ◽  
Blot Hybridization ◽  
Pathogenicity Island ◽  
Interleukin 8 ◽  
Middle Region ◽  
Dot Blot ◽  
Long Distance ◽  
H Pylori

ABSTRACT Helicobacter pylori virulence is associated with the presence of the cag pathogenicity island (PAI). Thecag PAI is involved in the ability to induce interleukin-8 (IL-8) secretion by human cells, which is implicated in the inflammatory response of the gastric mucosa to H. pyloriinfection. The aim of this study was to determine whether the genetic structure of the cag PAI is conserved and whether it is linked to IL-8 induction ability. Detection of specific markers (cagA, picB, cag13-cag14, virD4, and IS605) by PCR and dot blot hybridization and long-distance PCR determination of the presence of cagI, cagII, and the middle region of thecag PAI were performed on 153 strains isolated from adults suffering from ulcers (n = 79) or gastritis (n = 74). IL-8 induction ability was evaluated by coculture of the strains with HEp-2 cells. Eighty-three strains (54.3%) had an entire cag PAI, 12 strains (7.8%) had thecag PAI split in two, 49 strains (32%) had nocag PAI, and 9 strains exhibited other structural combinations. The presence of an entire cag PAI was statistically correlated with the presence of IS605(P = 0.006) and the ability to induce IL-8 secretion but not with clinical presentation of the infection. The structure of the cag PAI appears to be rather conserved and is related to the proinflammatory power of a strain. The existence of strains inducing IL-8 secretion regardless of the cag PAI structure suggests that this region is not the only requirement for IL-8 secretion.

scholarly journals Mucosal FOXP3-Expressing CD4+ CD25high Regulatory T Cells in Helicobacter pylori-Infected Patients

2005 ◽  
Vol 73 (1) ◽  
pp. 523-531 ◽  
Author(s):  
Anna Lundgren ◽  
Erika Strömberg ◽  
Åsa Sjöling ◽  
Catharina Lindholm ◽  
Karin Enarsson ◽  
...  
Keyword(s):  
T Cells ◽  
Helicobacter Pylori ◽  
Regulatory T Cells ◽  
Gastric Adenocarcinoma ◽  
Cytotoxic T Lymphocyte ◽  
Duodenal Mucosa ◽  
Peptic Ulcers ◽  
Low Levels ◽  
H Pylori ◽  
And Function

ABSTRACT Helicobacter pylori chronically colonizes the stomach and duodenum and causes peptic ulcers or gastric adenocarcinoma in 10 to 20% of infected individuals. We hypothesize that the inability of patients to clear H. pylori infections is a consequence of active suppression of the immune response. Here we show that H. pylori-infected individuals have increased frequencies of CD4+ CD25high T cells in both the stomach and duodenal mucosa compared to uninfected controls. These cells have the phenotype of regulatory T cells, as they express FOXP3, a key gene for the development and function of regulatory T cells, as well as high levels of the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) protein. In contrast, mucosal CD4+ CD25low and CD4+ CD25− cells express little FOXP3 mRNA and low levels of the CTLA-4 protein. Mucosal CD4+ CD25high T cells are present in individuals with asymptomatic H. pylori infections as well as in duodenal ulcer patients. The frequencies of CD4+ CD25high cells are also increased in the stomachs of H. pylori-infected patients with gastric adenocarcinoma, particularly in cancer-affected tissues. These findings suggest that regulatory T cells may suppress mucosal immune responses and thereby contribute to the persistence of H. pylori infections.

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