Effect of Seed Priming on Germination Properties and Seedling Establishment of Cowpea (Vigna sinensis)

Early emergence and stand establishment of cowpea are considered to be the most important yield-contributing factors in rainfed areas. Laboratory tests and afield experiment were conducted in RCB design in 2011 at a research farm in Ramhormoz, Iran, to evaluate the effects of hydropriming (8, 12 and 16 hours duration) and halo priming (solutions of 1.5% KNO3 and 0.8% NaCl) on seedling vigor and field establishment of cowpea. Analysis of variance of laboratory data showed that hydropriming significantly improved germination rate, seed vigor index, and seedling dry weights. However, germination percentage for seeds primed with KNO3 and non-primed seeds were statistically similar, but higher than those for NaCl priming. Overall, hydropriming treatment was comparatively superior in the laboratory tests. Invigoration of cowpea seeds by hydropriming and NaCl priming resulted in higher seedling emergence and establishment in the field, compared to control and seed priming with KNO3. Seedling emergence rate was also enhanced by priming seeds with water, suggesting that hydropriming is a simple, low cost and environmentally friendly technique for improving seed and seedling vigor of cowpea.

Download Full-text

Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

International Journal of Life Sciences ◽

10.3126/ijls.v9i1.11919 ◽

2015 ◽

Vol 9 (1) ◽

pp. 6-11

Author(s):

Abolfazl Masoumi Zavariyan ◽

Mojtaba Yousefi Rad ◽

Mohsen Asghari

Keyword(s):

Drought Stress ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Silybum Marianum ◽

Randomized Design ◽

Seedling Length ◽

Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

Download Full-text

Muskmelon seed priming in relation to seed vigor

Scientia Agricola ◽

10.1590/s0103-90162004000100019 ◽

2004 ◽

Vol 61 (1) ◽

pp. 114-117 ◽

Cited By ~ 20

Author(s):

Warley Marcos Nascimento ◽

Fernando Antônio Souza de Aragão

Keyword(s):

Low Temperature ◽

Interaction Effect ◽

Cucumis Melo ◽

Seed Quality ◽

Germination Rate ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Cucumis Melo L ◽

Aged Seeds

A number of important factors may affect seed priming response, including seed quality. Effects of seed vigor on seed priming response were investigated using seed lots of two muskmelon (Cucumis melo L.) cultivars. Seeds of muskmelon, cvs. Mission and Top Net SR were artificially aged at 43°C for 0, 20 and 40 hours. Seeds were primed for six days in darkness at 25°C in KNO3 (0.35 mol L-1) aerated solution. Aged seeds germinated poorly at 17°C. Priming increased germination rate at 17 and 25°C and germination percentage at 17°C. An interaction effect on germination performance between vigor and priming was observed, especially at low temperature. Priming increased germination performance in seeds of low vigor, and the response was cultivar dependent.

Download Full-text

Influence of seed priming on germination characteristics of sorghum (Sorghum bicolor L. Moench)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/911/1/012086 ◽

2021 ◽

Vol 911 (1) ◽

pp. 012086

Author(s):

Ramlah Arief ◽

Fauziah Koes ◽

Oom Komalasari ◽

Fatmawati

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Primary Root ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Quality Parameters ◽

Seed Vigor ◽

Germination Characteristics ◽

Positive Effect

Abstract Seed priming or invigoration is presowing treatments of improving germination aimed to reduce the time from sowing to emergence and improving emergence uniformity. Research on seed priming was conducted Indonesian Cereal Research Institute (ICERI) seed laboratory from January until March 2021 to evaluate the effect of seed priming on sorghum seed vigor. Seed quality parameters includes germination percentages, germination rate, shoot and primary root length, seedling dryweight, Sorghum seed variety of Suri 4 were used as seed material. Results showed that among the priming treatments that give positive effect on germination percentage, germination rate, seedling dry weight were priming treatment with KNO3 1.5%.

Download Full-text

Seed priming effects on germination and first growth of the medicinal plant Achillea millefolium L.

Journal of Phytology ◽

10.25081/jp.2020.v12.6324 ◽

2020 ◽

pp. 20-23

Author(s):

Panagiotis Kanatas ◽

Vyronas Dellaportas ◽

Ioanna Kakabouki ◽

Panayiota Papastylianou

Keyword(s):

Control Experiment ◽

Germination Rate ◽

Seedling Emergence ◽

Seed Priming ◽

Potassium Nitrate ◽

Potential Effect ◽

Germination Percentage ◽

Priming Effects ◽

Positive Effects ◽

Germination And Growth

This study evaluated the effects of seed priming on germination and growth of A. millefolium by means of laboratory and greenhouse experiments conducted during 2018 in the Agricultural University of Athens. Treatments were GA3 (400 and 800 ppm), potassium nitrate (2% and 4%), polyethylene-glycol (soaking for 12 and 24h) besides an untreated control. Experiment in Petri dishes revealed that GA3 at 400 ppm, potassium nitrate (at concentration 2 and 4%) and PEG significantly increased germination percentage of A. millefolium, while germination rate was also significantly improved as a result of all seed priming techniques. In addition, due to the soil experiment, seedling emergence was significantly increased by GA3 at 400 ppm, potassium nitrate (at both concentrations) and PEG compared with the untreated seeds. Dry biomass of the young seedlings was significantly enhanced by means of GA3 (at 400 and 800 ppm), KNO3 (4%) and PEG for 24 h, indicating the potential effect of seed priming on first growth as well. The results of the present study revealed the significant positive effects of seed priming on A. millefolium seed germination, seedling emergence and early growth.

Download Full-text

SEED QUALITY EFFECTS ON HYDROPONIC LETTUCE

HortScience ◽

10.21273/hortsci.31.6.912d ◽

1996 ◽

Vol 31 (6) ◽

pp. 912D-912

Author(s):

Ahmet Korkmaz ◽

Wallace Pill

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Seedling Emergence ◽

Seed Priming ◽

Germination Percentage ◽

Fresh Weight ◽

Seedling Length ◽

Size Uniformity ◽

Initial Seed ◽

Final Harvest

Achievement of head size uniformity at final harvest reduces loss and increases profitability for the hydroponic lettuce grower. Shoot fresh weight of `Cortina' lettuce (Lactuca sativa L.) at 7 or 21 days after planting (DAP) was inversely proportional to the number of days required for seedling emergence, and was greater for raw than for pelleted seeds. Head fresh weight at final harvest (61 DAP) was directly proportional to seedling length at 21 DAP, but raw and pelleted seeds produced equal head weights. Thus, initial seed (seedling) vigor differences were maintained to final harvest. Osmotic seed priming (–1.5 MPa KH2PO4, 20 h, dark) led to increased germination rate at 15, 25, and 35C; had no effect on germination synchrony; and increased germination percentage only at 35C. Covering raw or pelleted seeds sown in depressions of the phenolic foam trays with fine (No. 5) vermiculite compared to leaving the seeds uncovered, and soaking the trays in hydroponic solution rather than water, increased seedling shoot fresh weights. Seeds sown on their first day of germination or primed seeds gave greater seedling shoot fresh weights than pelleted seeds. However, the more uniform seedling shoot fresh weights from germinated seeds than from primed seeds was associated with more rapid and synchronous seedling emergence.

Download Full-text

The Effect of Seed Priming and Accelerated Aging on Germination and Physiochemical Changes in Milk Thistle (Silybum marianum)

Notulae Scientia Biologicae ◽

10.15835/nsb529017 ◽

2013 ◽

Vol 5 (2) ◽

pp. 204-211 ◽

Cited By ~ 5

Author(s):

Ghasem PARMOON ◽

Ali EBADI ◽

Sodabeh JAHANBAKHSH ◽

Mahdi DAVARI

Keyword(s):

Polyphenol Oxidase ◽

Seedling Growth ◽

Germination Rate ◽

Accelerated Aging ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Milk Thistle ◽

Antioxidants Activity ◽

Germination Speed

Effects of seed priming and aging on some physiological characteristics of Milk thistle was studied in a factoral experiment based on Complete Randomized Design (CRD). Tratments were included hydro priming (using distilled water), halo priming (0, 1.5, 3, 4.5 and 6% KNO3) and accelerated aging (0, 2, 4 and 6 days under 45°C and 95% humidity) in three replications. Determined parameters were germination charactristics including germination percentage, daily germination speed, mean time of germination, seed vigor index, hypocutile length and hypocutile dry weight. Activity of catalase, peroxidase and polyphenol oxidase were determined at 12 hours after imbibition and seedling stage. According to results of this experiment, germination percentage, seed vigor and seedling growth of seeds were increased under all priming treatments. Improving the catalase and peroxidase activity led to decrease the aging damages. Germination characteristics were improved under both priming treatments at the beginning of germination as well as seedling growth. Polyphenol oxidase activity was increased in the pre-treated seeds but decreased in seedling growth stage. Aging treatments led to reduce the germination percentage, daily germination speed, seed vigor and seedling growth while the germination time was increased. Accelerated aging caused to reduce the germination rate and seedling growth of milk thistle that is probably due to increasing the lipid peroxidation, free radical increment and decreasing the antioxidants activity. The greatest and lowest antioxidants activity, the germination percentage, germination speed and seed vigor were respectively observed under priming using 3% KNO3 concentration and control seeds.

Download Full-text

Effect of seed priming by pyridoxine on germination and biochemical indices in Silybum marianum L. under drought stress

International Journal of Life Sciences ◽

10.3126/ijls.v9i1.11921 ◽

2015 ◽

Vol 9 (1) ◽

pp. 17-22 ◽

Cited By ~ 1

Author(s):

Abolfazl Masoumi Zavariyan ◽

Mojtaba Yousefi Rad ◽

Mohsen Asghari

Keyword(s):

Drought Stress ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Silybum Marianum ◽

Randomized Design ◽

Biochemical Indices ◽

Germination Indices

In order to investigation of the effect of seed priming by pyridoxine on germination and biochemical indices of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of pyridoxine including 0, 0.02% and 0.04% and different levels of drought stress including 0, -6 and -12 bar at three replicates was performed. The results indicated that drought stress affected measured indices at P<0.01 and pyridoxine significantly affected germination percentage and rate, seed vigor, mean time germination, seedling length and dry weight at P<0.01 and peroxidase activity at P<0.05. Interaction between the drought stress and pyridoxine was significantly (P<0.05) affected of germination rate and seed vigor. The results showed that with increasing drought stress, germination indices were decreased and biochemical indices were increased. Seed priming with pyridoxine cause to improvement of germination indices and decreases biochemical indices. According to the results, seed priming with 0.04% pyridoxine cause to improvement of germination indices at drought stress conditions.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11921 International Journal of Life Sciences Vol.9(1) 2015 17-22

Download Full-text

The Effect of Priming on Germination and Growth Indices in Chamran Wheat Variety in North of Khuzestan Province, Iran

Cercetari Agronomice in Moldova ◽

10.1515/cerce-2017-0022 ◽

2017 ◽

Vol 50 (3) ◽

pp. 15-24

Author(s):

Sh. Lorzadeh ◽

M. Sharafizadeh ◽

M.R. Enayat Gholizadeh

Keyword(s):

Salicylic Acid ◽

Acid Treatment ◽

Germination Rate ◽

Germination Percentage ◽

Seed Vigor ◽

Control Treatment ◽

Distilled Water ◽

Seedling Vigor ◽

Salicylic Acid Treatment ◽

Vigor Index

Abstract In order to study the plant growth regulator, using salicylic acid (SA) on germination and growth indices for laboratory research in crop year 2014-2015, which was conducted at the research center Safiabad-Dezful, Khuzestan province. The treatments included four levels of priming with salicylic acid {control (distilled water), 0.7, 1.2, 1.7 mM} in a completely randomized design in three replications. The results showed that the control treatment reduced the decrease in germination time. The highest percentage of germination and related characteristics of the control (distilled water) was obtained. Seed vigor and seedling vigor index were reduced in high concentrations of salicylic acid. The germination percentage of an average daily germination, seed vigor and seedling vigor index had a positive and significant correlation with the daily germination rate showed a significant negative correlation. The results of comparison of treatments showed that the treatment concentration of 0.7 mM salicylic acid highest (22.3 days) and control (distilled water) lowest (0.3 day) had mean germination time. Effects of priming showed the highest mean daily germination (15.44) related to the control and the lowest (2.97) related to the concentration of 7.1 mM salicylic acid. Also, the results showed that the 1.7 mM salicylic acid treatment had the highest number of germinated seeds per day (0.3880) and control treatment had the least number of germinated seeds per day (0.6467). The highest vigor index was observed in control treatment with seedling length of (8.15 cm) and the lowest vigor index was observed in 1.7 mM salicylic acid treatment with seedling length of (1.54 cm). The highest seedling vigor index was observed in control treatment (33.58) and the lowest seedling vigor index was observed in 1.7 mM salicylic acid treatment (17.20). The coefficient velocity germination rate in control treatment was highest (3) and in the 1.7 mM salicylic acid treatment was lowest (0.15). The correlation coefficient between vigor index and seedling vigor index has the highest value (98%). There was a positive correlation between germination percentage and mean daily germination and there was a negative correlation between germination percentage and daily germination speed.

Download Full-text

EFFECT OF SEED PRIMING ON GERMINATION OF OKRA (Abelmoschus esculentus var. Arka Anamika)

Malaysian Journal of Sustainable Agricultural ◽

10.26480/mjsa.02.2021.111.114 ◽

2021 ◽

Vol 5 (2) ◽

pp. 111-114

Author(s):

Anuj Lamichhane ◽

Mamata K.C. ◽

Manisha Shrestha ◽

Binaya Baral

Keyword(s):

Seed Germination ◽

Tap Water ◽

Germination Rate ◽

Seed Priming ◽

Seed Vigor ◽

Seedling Vigor ◽

Randomized Design ◽

Vigor Index ◽

Seed Hardness ◽

Positive Effect

Seed priming is an effective, eco-friendly method to promote seed germination and seedling vigor of okra to overcome the reduced and delayed germination in fresh or stored okra seeds caused by seed hardness. An experiment was carried to evaluate the effects of different priming on okra seeds germination and seedling vigor using Arka Anamika variety at Horticulture lab of Agriculture and Forestry University, Rampur, Chitwan, Nepal. Investigation was carried out with 6 treatments (T1: seed priming with tap water, T2: seed priming with 200ppm NAA solution, T3: seed priming with 10% PEG-200 solution, T4: seed priming with 200ppm GA3 solution, T5: seed priming with 5% Trichoderma solution and T6 no priming) with 4 replications in Complete Randomized Design (CRD). Seeds primed with T1 to T5 were soaked for 24 hours and shade dried for 6 hours before sowing. Priming with T4 was found to be best in terms of maximum seed germination (60.12%), seed vigor index (5772.68 cm), mean germination rate (7.53 seeds per day). The highest shoot length (81.40 mm) was observed at T1 whereas enhancement of root length occurred with the priming with T3. All treatments had a significant positive effect on all the germination parameters in comparison to control. The study concluded that GA3 priming enhanced germination as well as seed vigor in okra and hydro priming and tricho-priming can be used as an alternative to GA3 priming among farmers in Nepal.

Download Full-text

Using seed respiration as a tool for calculating optimal soaking times for ‘on-farm’ seed priming of barley (Hordeum vulgare)

Seed Science Research ◽

10.1017/s0960258521000039 ◽

2021 ◽

pp. 1-9

Author(s):

Javier Carrillo-Reche ◽

Adrian C. Newton ◽

Richard S. Quilliam

Keyword(s):

Morphological Changes ◽

Low Cost ◽

Germination Rate ◽

Seed Priming ◽

Embryonic Axes ◽

Seedling Vigour ◽

Soaking Time ◽

On Farm ◽

Seed Respiration

Abstract A low-cost technique named ‘on-farm’ seed priming is increasingly being recognized as an effective approach to maximize crop establishment. It consists of anaerobically soaking seeds in water before sowing resulting in rapid and uniform germination, and enhanced seedling vigour. The extent of these benefits depends on the soaking time. The current determination of optimal soaking time by germination assays and mini-plot trials is resource-intensive, as it is species/genotype-specific. This study aimed to determine the potential of the seed respiration rate (an indicator of metabolic activity) and seed morphological changes during barley priming as predictors of the priming benefits and, thus, facilitate the determination of optimal soaking times. A series of germination tests revealed that the germination rate is mostly attributable to the rapid hydration of embryo tissues, as the highest gains in the germination rate occurred before the resumption of respiration. Germination uniformity, however, was not significantly improved until seeds were primed for at least 8 h, that is, after a first respiration burst was initiated. The maximum seedling vigour was attained when the priming was stopped just before the beginning of the differentiation of embryonic axes (20 h) after which vigour began to decrease (‘over-priming’). The onset of embryonic axis elongation was preceded by a second respiration burst, which can be used as a marker for priming optimization. Thus, monitoring of seed respiration provides a rapid and inexpensive alternative to the current practice. The method could be carried out by agricultural institutions to provide recommended optimal soaking times for the common barley varieties within a specific region.

Download Full-text