scholarly journals Peroxidase activity and initial growth of ‘Barbosa’ peach on clonal rootstocks

2019 ◽  
Vol 41 (6) ◽  
Author(s):  
Chirlene Márcia Oldoni ◽  
Alexandre Augusto Nienow ◽  
Jurema Schons ◽  
Newton Alex Mayer
Keyword(s):  
Peroxidase Activity ◽  
Diameter Growth ◽  
Initial Growth ◽  
Trunk Diameter ◽  
Dormancy Period ◽  
Nursery Trees ◽  
Survival And Growth ◽  
Graft Compatibility ◽  
Graft Incompatibility ◽  
Canning Industry

Abstract In the peach nursery trees production, the use of rootstocks with unknown genetic identity obtained from peach seeds in the canning industry is frequent. The hypothesis tested was that there are rootstocks that express greater graft compatibility, enabling greater survival and growth, and that peroxidase activity can be used as indication of graft incompatibility. The aim of this study was to evaluate the survival and the peroxidase activity efficiency in identifying graft incompatibility and the trunk diameter growth of ‘Barbosa’ peach grafted onto 18 Prunus clonal rootstocks, propagated by herbaceous cuttings, compared to own-rooted scion trees. The experimental design was three randomized blocks, with one tree per plot. The first three vegetative cycles of trees conducted in double Y (5.0m x 2.0m) without irrigation were evaluated. The highest trunk diameter growth was provided by ‘Okinawa’, ‘Tsukuba-1’, ‘Tsukuba-2’, Mexico F1 and ‘Flordaguard’ rootstocks, without differing from own-rooted ‘Barbosa’ peach. Peroxidase activity differs by rootstock effect at the beginning of the dormancy period, and is higher than in vegetative growth, especially in interspecific graft combinations. Peroxidase activity is a biochemical indicator of stress, but should not be used alone and generalized to characterize graft incompatibility.

scholarly journals A Method for Prediction of Graft Incompatibility in Sweet Cherry

2012 ◽  
Vol 40 (1) ◽  
pp. 243 ◽  
Author(s):  
S. Filiz GUCLU ◽  
Fatma KOYUNCU
Keyword(s):  
Peroxidase Activity ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Ground Tissue ◽  
Tissue Samples ◽  
Total Peroxidase Activity ◽  
Graft Incompatibility ◽  
Old Trees

This study was carried out on 1-year old trees of ‘0900 Ziraat’ variety grafted onto ‘Kuş kirazi’, ‘Kara idris’, ‘Sari idris’, ‘MaxMa 14’, ‘MaxMa 60’ and ‘Gisela 5’ in order to determine their compatibility. For this purpose, total peroxidase activity was determined by spectrophotomery assay. Taking ground tissue samples were planned three times as before the grafting (beginning), and then 8 and 12 months after grafting. Total peroxidase activities of rootstocks before grafting varied between 10.80 ΔAg.min. (‘Kuş kirazi’) and 7.83 ΔAg.min. (‘Kara idris’) and were found to be statistically important. The peroxidase activity of ‘0900 Ziraat’ was 11.07ΔAg.min. and the closest value occurred in Prunus avium rootstock. The most different values occurred in rootstocks of ‘Kara idris’ and ‘Gisela 5’. The results showed that peroxidase activity increased in rootstock and graft scion. This increase had higher values in heterogenetic combinations especially in ‘0900 Ziraat’/‘Gisela 5’ and ‘0900 Ziraat’/‘Kara idris’ grafts. Peroxidase activity was decreased at the 12th month for all combinations. The highest value was obtained from ‘0900 Ziraat’/‘MaxMa 14’ combinaion with 29.17 ΔAg.min. while lowest one was ‘0900 Ziraat’/‘Kuş kirazi’ with 17.39. The findings showed that peroxidase activity could be used as a parameter in early determination of graft incompatibility.

scholarly journals Effect of fungicides on the enzymatic activity of the antioxidant system and the chlorophyll content in lupine plants during seed dressing

2020 ◽  
pp. 3-6
Author(s):  
O. Borzykh ◽  
O. Tsurkan ◽  
L. Chervyakova ◽  
T. Panchenko
Keyword(s):  
Analytical Chemistry ◽  
Chlorophyll Content ◽  
Peroxidase Activity ◽  
Catalase Activity ◽  
Control Level ◽  
Growth Stages ◽  
Enzymatic System ◽  
Initial Growth ◽  
Seed Dressing ◽  
Catalase Peroxidase

Goal. The effect of fungicides on the dynamics of the activity of peroxidase, catalase (CAT) and chlorophyll content in lupine plants during seed dressing has been established. Methods. Laboratory and vegetation researches were conducted in the laboratory of analytical chemistry of pesticides of the Institute of Plant Protection. Yellow lupine (Lupinus luteus L.), variety Obriy has been grown. The objects of research were fungicides triticonazol (40 g/t) and its combination with prochloraz (120 g/t). Determination of the content of fungicides in plants was carried out using chromatographic methods according to officially approved methods and me­thods developed in the laboratory of analytical chemistry of pesticides. Chlorophyll content and peroxidase activity were measured by colorimetric method, catalase activity — by titrimetric method. Results. According to the research results, the varying sensitivity of the enzymatic system of antioxidant defense (catalase, peroxidase) in response to seed dressing by fungicides was recorded. It showed that on the 10th day after sowing, content of triticonazol in plants was 0.8 mg/kg, and the peroxidase activity was similar to that in untreated plants. Subsequently, against the background of a decrease in the content of the active substance, a gradual activation of the enzyme was observed. Catalase activity also gradually increased beginning from the 14th day, and on the 30th day it exceeded the corresponding control indicator by 40%. When using a combination of triticonazol with prochloraz, the disturbance in the balance of peroxidase catalase was more significant. However, by the phase of 7—8 leaves, with a minimal total content of fungicides (0.38 mg/kg), the enzyme activity approached the control level, which is associated with the restoration of plant homeostasis and the formation of its adaptive potential under stress conditions. The stimulating effect of these fungicides on chlorophyll content at the initial growth stages of lupine was established. The chlorophyll concentration in fungicides-treated plants exceeded the control indicator by 11—29%. Conclusions. The use of systemic triazole fungicides to protect seedlings, improves the photosynthetic activity of plants and at the same time acts as a stress factor that activates protecting enzymes (catalase, peroxidase), which trigger the development of protective adaptive reactions of plants.

scholarly journals Naturally Seeded versus Planted Ponderosa Pine Seedlings in Group-Selection Openings

2009 ◽  
Vol 24 (1) ◽  
pp. 48-54 ◽  
Author(s):  
Philip M. McDonald ◽  
Gary Fiddler ◽  
Martin Ritchie ◽  
Paula Anderson
Keyword(s):  
Growth Rate ◽  
Plant Community ◽  
Ponderosa Pine ◽  
Natural Regeneration ◽  
Group Selection ◽  
Average Density ◽  
Stem Diameter ◽  
Diameter Growth ◽  
Pine Seedlings ◽  
Survival And Growth

Abstract The purpose of this article was to determine whether natural regeneration or planted seedlings should be used in group-selection openings. The answer depends on the survival and growth rate of both types of seedlings, and that could depend on the size of the openings and the effect of trees on their edge. In this side-by-side study, the natural pine seedlings originated from the 1988 seed crop and the 1–0 nursery-grown seedlings were outplanted in spring 1989. Openings ranged from 0.01 to 0.65 ha. The plant community consisted of many species of shrubs, forbs, and grasses with manzanita having the highest density and greatest development. After 9 years, manzanita had an average density of 13,870 plants/ha, 2,050 m2/ha of foliar cover, and was 125 cm tall. From 1990 to 1997, planted ponderosa pine seedlings were taller (P < 0.05) than natural seedlings, and from 1995 to 1997, mean stem diameter at 30 cm of planted seedlings was larger than natural counterparts (P < 0.05). Development for 1 year in the nursery apparently gave the planted seedlings a growth advantage over the natural seedlings. For natural seedlings, distance from opening edge had little effect on pine height or diameter growth regardless of opening size. Planted seedlings, however, appeared to increase in height and diameter growth with both opening size and distance from edge.

Effects of Rootstock Source on the Size, Cone Production, and Compatibility of Douglas-Fir Grafts

1992 ◽  
Vol 7 (3) ◽  
pp. 73-77
Author(s):  
Ingemar Karlsson ◽  
Jack H. Woods
Keyword(s):  
Pseudotsuga Menziesii ◽  
Douglas Fir ◽  
Fast Growth ◽  
Production Potential ◽  
Graft Union ◽  
Cone Production ◽  
Graft Compatibility ◽  
Graft Incompatibility

Abstract Scions of coastal Douglas-fir (Pseudotsuga menziesii) were grafted on rootstock from coastal, northern interior and southern interior origin. Data on ramet size, cone production, and graft compatibility were collected. Twelve years after grafting significant differences for most crown and stem traits were found among the rootstock sources, with coastal rootstock resulting in the fastest scion growth. Northern interior rootstock sources produced larger grafts than southern interior rootstock. Significant graft union appearance and cone production differences were not detected, but coastal sources consistently had higher graft compatibility and healthier unions. It is concluded that, for coastal Douglas-fir, the use of a vigorous coastal rootstock source that promotes fast growth in the scion, and has high graft compatibility potential, is the most desirable for coastal locations. This will result in fewer losses due to graft incompatibility, faster crown development, more options for crown management and increased cone production potential. West. J. Appl. For. 7(3):73-77.

scholarly journals Long-Term Seedfall, Establishment, Survival, and Growth of Natural and Planted Ponderosa Pine in the Colorado Front Range

2006 ◽  
Vol 21 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Wayne D. Shepperd ◽  
Carleton B. Edminster ◽  
Stephen A. Mata
Keyword(s):  
Ponderosa Pine ◽  
Basal Area ◽  
Colorado Front Range ◽  
Initial Growth ◽  
Front Range ◽  
Natural Seedling ◽  
Survival And Growth ◽  
Seed Crops ◽  
Better Than

Abstract Seedfall, natural regeneration establishment, and growth of planted seedlings was observed from 1981 to 2001 under shelterwood and seedtree overstories in a replicated study in ponderosa pine in the Manitou Experimental Forest in the Colorado Front Range. Good seed crops were produced only every 4 to 6 years, with almost no viable seed produced in intervening years. With seed predation, only 14% of total seedfall was available for germination. Shelterwood overstories containing between 6 and 14 m2 ha−1 stem basal area over scarified seedbeds provided optimal conditions for natural seedling establishment. Survival and growth of planted seedlings was much better than that of natural seedlings. However, poor survival and slow initial growth may require many years to establish a fully stocked forest of natural seedlings.

scholarly journals Use of Plastic Shelters for Low-Cost Establishment of Street Trees

1996 ◽  
Vol 20 (2) ◽  
pp. 85-89 ◽  
Author(s):  
R. H. Jones ◽  
A. H. Chappelka ◽  
D. H. West
Keyword(s):  
Tree Species ◽  
Low Cost ◽  
Height Growth ◽  
Diameter Growth ◽  
Industrial Park ◽  
Growth Responses ◽  
Urban Settings ◽  
Planting Methods ◽  
Survival And Growth ◽  
One Year

Abstract One-year-old seedlings of 11 commonly used urban shade tree species were protected with plastic shelters to determine effects on survival and growth. Additional seedlings were planted in the cities of Auburn and Opelika, Alabama, to determine seedling performance in actual urban settings and to estimate incidence of vandalism in five urban settings. Shelters increased survival in four species and height growth in seven. Diameter growth responses were mixed. During the first 13 months after planting, only 3% of the seedlings in the cities were damaged by people. However, 20% of the shelters were vandalized at least once. Vandalism rates for shelters were greatest (37-44%) in neighborhoods of privately and publicly owned homes; intermediate (20%) in recreational parks, and lowest (4-6%) in undeveloped or industrial park areas. Installation of each tree with its shelter cost $2.78 (excluding labor) and required 20-25 minutes of labor. Tree shelters show promise as a low-cost alternative to more expensive planting methods, especially in undeveloped portions of cities. South. J. Appl. For. 20(2):85-89.

scholarly journals Soil-applied ZnEDTA: Vegetative Growth, Nut Production, and Nutrient Acquisition of Immature Pecan Trees Grown in an Alkaline, Calcareous Soil

HortScience ◽  
2017 ◽  
Vol 52 (2) ◽  
pp. 301-305 ◽  
Author(s):  
James L. Walworth ◽  
Scott A. White ◽  
Mary J. Comeau ◽  
Richard J. Heerema
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Calcareous Soils ◽  
Carya Illinoinensis ◽  
Additional Benefit ◽  
Zn Deficiency ◽  
Diameter Growth ◽  
Trunk Diameter ◽  
The Third ◽  
Nut Production ◽  
Root Tissues

A field study was conducted to evaluate efficacy of soil-applied zinc (Zn) fertilizer on young pecan [Carya illinoinensis (Wangenh.) K. Koch] trees growing in alkaline, calcareous soils. Chelated Zn ethylenediaminetetraacetic acid (ZnEDTA) was applied at rates of 0, 2.2, or 4.4 kg·ha−1 of Zn via injection into irrigation water (fertigation) in microsprinkler irrigated ‘Western’ and ‘Wichita’ trees. Over the 5-year duration of the study, leaf Zn levels were increased from 22 to 35 µg·g−1 in the highest rate of ZnEDTA treatment compared with 7 to 14 µg·g−1 in unfertilized trees. Zn concentrations in shoot and root tissues were also elevated in Zn-treated trees. Zn treatments largely eliminated visible Zn deficiency symptoms, and increased trunk diameter growth compared with untreated trees. Nut yield (in the third through fifth seasons) were also increased as a result of Zn fertilization. No additional benefit in terms of trunk diameter growth or nut yield was observed by adding a higher rate of Zn (4.4 kg·ha−1) vs. the lower rate (2.2 kg·ha−1). ‘Western’ and ‘Wichita’ trees responded similarly to Zn fertigation.

scholarly journals Effect of Cyclic Irrigation on Growth of Magnolias Produced Using Five In-ground Systems

2003 ◽  
Vol 21 (3) ◽  
pp. 148-152
Author(s):  
R. C. Beeson ◽  
K. Keller
Keyword(s):  
Growth Rates ◽  
Shoot Growth ◽  
Similar Rate ◽  
Height Growth ◽  
Irrigation Event ◽  
The Other ◽  
Diameter Growth ◽  
Trunk Diameter ◽  
Magnolia Grandiflora ◽  
Ground Systems

Abstract Five in-ground systems were evaluated based on shoot growth and marketability for the production of 2.17 m (7 ft) tall and 5.08 cm (2 in) trunk diameter Magnolia grandiflora cv. ‘Symmes Select’. Systems evaluated were Root Control Bags, Geo-Cell bags, and #25 containers in pot-in-pot in Experiment 1, and Agro-liners in socket pots and directly in-ground in Experiment 2. Each system was either cyclically irrigated or given a single irrigation event daily. In Experiment 1, cyclic irrigation increased the rate of height growth and trunk diameter compared to a single irrigation. Trees in Root Control Bags and pot-in-pot grew at a similar rate and faster than those in Geo-Cell bags. Faster growth rates with cyclic irrigation resulted in earlier marketability compared to single irrigation daily, except for pot-in-pot. In Experiment 2, cyclic irrigation increased the rate of trunk diameter growth, and Agro-liners in pots had greater height and trunk diameter growth rates than those directly in ground. Trees grown in the in-ground pot systems, whether in Agro-liners or black polyethylene containers, grew similarly to trees in Root Control Bags. Trees grown in Geo-cells or Agro-liners in-ground had significantly slower growth than the other treatments.

scholarly journals Compatibility Evaluation and Anatomical Observation of Melon Grafted Onto Eight Cucurbitaceae Species

2021 ◽  
Vol 12 ◽  
Author(s):  
Mu Xiong ◽  
Changjin Liu ◽  
Liping Guo ◽  
Jin Wang ◽  
Xiangshuai Wu ◽  
...  
Keyword(s):  
Cucumis Melo ◽  
Dry Weight ◽  
Bottle Gourd ◽  
Bitter Gourd ◽  
Starch Accumulation ◽  
Callose Deposition ◽  
Stem Base ◽  
Graft Compatibility ◽  
Graft Incompatibility ◽  
Wax Gourd

Melon (Cucumis melo) is one of the top 10 fruits in the world, and its production often suffers due to soil-borne diseases. Grafting is an effective way to solve this problem. However, graft incompatibility between scion and rootstock limits the application of melon grafting. In this study, the melon was grafted onto eight Cucurbitaceae species (cucumber, pumpkin, melon, luffa, wax gourd, bottle gourd, bitter gourd, and watermelon), and graft compatibility evaluation and anatomical observation were conducted. Taking melon homo-grafted plants as control, melon grafted onto cucumber and pumpkin rootstocks was compatible, while melon grafted onto luffa, wax gourd, bottle gourd, bitter gourd, and watermelon rootstocks was incompatible based on the scion dry weight on day 42 after grafting. Meanwhile, we found that starch–iodine staining of scion stem base is an index to predict graft compatibility earlier, on day 14 after grafting. Further, microsection observations showed that there was more cell proliferation at graft junction of melon hetero-grafted combinations; vascular reconnection occurred in all graft combinations. However, excess callose deposited at graft junction resulted in the blockage of photosynthate transport, thus, leading to starch accumulation in scion stem base, and finally graft incompatibility. In addition, undegraded necrotic layer fragments were observed at graft junctions of melon grafted onto incompatible bitter gourd and watermelon rootstocks. The above results provide clues for the selection and breeding of compatible Cucurbitaceae rootstocks of melon and demonstrate that starch accumulation in scion base and callose deposition at graft junction is associated with melon graft compatibility.

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