scholarly journals ELECTRICAL CONDUCTIVITY IN Piptadenia moniliformis Benth. SEED LOTS CLASSIFIED BY SIZE AND COLOR

2016 ◽  
Vol 40 (5) ◽  
pp. 855-866
Author(s):  
Gilvaneide Alves Azeredo ◽  
Rinaldo César de Paula ◽  
Sérgio Valiengo Valeri

ABSTRACT Among the most widely used tests for evaluating seed vigor, electrical conductivity (EC) stands out as one of the most promising due to its speed and simplicity. This study aimed to verify if the EC test could be used to evaluate the physiological quality of seed lots of Piptadenia moniliformis Benth. A seed lot, which was classified by seed tegument color and size, was used based on seed retention in round-hole screens, sizes 11 and 15, constituting 10 sub-lots. Prior to germination and the EC test, the seeds were immersed in concentrated sulfuric acid for 20 minutes in order to overcome tegument dormancy. During the EC test, we soaked seeds in 75 mL of water at 25 ºC and tested eight soaking periods (2, 4, 6, 24, 48, 72, 96, and 120 h) and two seed quantities (25 and 50) per replicate. The EC test, regardless of the number of seeds or soaking period, was not adequate for evaluating the physiological quality of color- or size-classified seed lots from P. moniliformis Benth. .

2020 ◽  
Vol 42 ◽  
pp. e51287
Author(s):  
Joyce Naiara da Silva ◽  
Monalisa Alves Diniz da Silva

 The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformis Benth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but the germination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformis after 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformis into concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated aging at 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.


2021 ◽  
Vol 29 ◽  
pp. 28-35
Author(s):  
Letícia Betânia Xavier Dias ◽  
Pedro Afonso de Melo Queiroz ◽  
Thaís Cardoso de Castro ◽  
Marco Antonio Moreira de Freitas ◽  
Érica Fernandes Leão-Araújo ◽  
...  

Our objective was to evaluate the occurrence of mechanical damage in chickpea seeds with the combination of two harvester speeds (2.5 and 3.5 km.h-1) and three adjustments for rotor rotation speeds (500, 700, and 850 rpm). Harvesting was carried out in a seed production field. Seeds were evaluated for purity, germination (G), first count (FC), germination speed index (GSI), hypochlorite, electrical conductivity, and tetrazolium tests. There was an effect of harvester speeds on seed physiological quality for the first count (FC), germination (G), germination speed index (GSI), especially when combining with high rotor rotation speed. In these cases, the speed of 2.5 km.h-1 resulted in lower values. This harvester speed also had worse results when combining with 850 rpm for purity and hypochlorite tests. The tetrazolium test was not efficient in identifying differences in seed quality. There was no significant interaction between harvester speeds and rotor rotation speeds for the conductivity test. Evaluating the harvester speed’s isolated effect (3.5 km.h-1), we identified problems in seed vigor due to the higher value of exudates in the electrical conductivity test. Low harvester speed (2.5 km.h-1) associated with high rotor rotation speeds (700 and 850 rpm) causes a reduction of the physical and physiological quality of seeds.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.


2012 ◽  
Vol 30 (4) ◽  
pp. 695-698 ◽  
Author(s):  
Cristiaini Kano ◽  
Antonio Ismael Inácio Cardoso ◽  
Roberto L Villas Bôas

Because of lack of information about phosphorus fertilization in lettuce from the standpoint of seed production, this study was undertaken. The work was carried out in Botucatu, São Paulo state, Brazil, from September 25, 2003 to February 19, 2004, in order to study the influence of crescents phosphorus rates on yield and quality of lettuce seeds, cultivar Verônica. The experimental design was randomized blocks with five treatments (0; 200; 400; 600 and 800 kg ha-1 of P2O5) and five replications. The following characteristics were evaluated: seed production per plant (g plant-1), number of seeds per plant and quality (percentage of germination and seed vigor). A linear increasing was observed on production and number of seeds per plant of lettuce with phosphorus rates, but these rates did not affect the seed physiological quality evaluated on weight of 1,000 seeds (average = 0.91 g), first count of germination (98%), standard germination test (99%), seed germination speed index (68.5), seedling emergence speed index (97.0) and emerged seedlings (49.3%).


2019 ◽  
Vol 86 ◽  
Author(s):  
Vanessa Nogueira Soares ◽  
Francisco Amaral Villela ◽  
Aline Klug Radke ◽  
Hélen Claudine Saliba Rodrigues ◽  
Vanessa Pinto Gonçalves ◽  
...  

ABSTRACT The physiological performance of seeds is related to their physiological quality and seed vigor, while their health quality may interfere with germination and early seedling establishment in the field due to the interaction of microorganisms associated with seeds. The study aimed to evaluate the physiological performance and health quality of cucumber seeds and to verify the relationship between these attributes. The physiological quality of cucumber seeds was evaluated by standard germination tests, first count of germination, controlled deterioration test, electrical conductivity test, seedling emergence, emergence speed index, and traditional accelerated aging and aging modified with saline for 48, 72, and 96 h. The health quality of cucumber seed lots was evaluated by blotter test. Stratification of cucumber seed lots by seedling emergence was similar to seedling emergence by controlled deterioration test, first count of germination, and electrical conductivity results. The cucumber seed lots evaluated showed high germination rates; however, lots 1 and 3 had a better performance in vigor tests than lots 2 and 4. Fungi detected in the blotter test were Alternaria sp., Aspergillus sp., Cladosporium sp., and Penicillium sp. Seed lots 2 and 4 had low vigor evaluated by seedling emergence and controlled deterioration, and showed a higher incidence of Penicillium sp. in the evaluation of health quality of seeds. The incidence of Penicillium sp. may negatively affect the vigor of cucumber seeds evaluated by seedling emergence and by controlled deterioration test.


2018 ◽  
Vol 10 (3) ◽  
pp. 377
Author(s):  
A. K. Radke ◽  
D. W. Maass ◽  
M. S. Pieper ◽  
F. M. Xavier ◽  
A. B. N. Martins ◽  
...  

Chia (Salvia hispanica L.) is an herbaceous plant that belongs to the Lamiaceae family. Seed vigor testing is an important component of quality control programs, and electrical conductivity test is a possible option in this process. The objective of this study was to identify a methodology of electrical conductivity test to stratify chia seed lots at vigor level according to the emergence of seedlings. Six lots of chia seeds were used in this study. The seeds were exposed to the following determinations: first germination count, germination, emergence speed index, emergence, aerial and root length, aerial and root dry matter, and electrical conductivity at 25, 50 and 75 mL during 1, 2, 4, 6, 8 and 24 hours of soaking in water. The electrical conductivity test in the combination of 25 seeds using 50 mL of water in 6-hour of soaking period, as well as the combination of 50 seeds using 50 mL of water for 24 hours of soaking, were efficient in the stratification of chia seeds in different levels of vigor.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano

<p>Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe <em>Hylocereus undatus </em>fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p &lt; 0.05). The germination test for <em>H. undatus </em>seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.</p>


2021 ◽  
Vol 6 (1) ◽  
pp. 100-107
Author(s):  
Gilvaneide Alves de Azerêdo ◽  
Rinaldo Cesar de Paula

The accelerated aging test is efficient for evaluating the seed vigor of many  species, but there are few studies on forest tree species. The aim of this study was to determine the adequate exposure time of Piptadenia moniliformis Benth. seeds to the accelerated aging test in order to evaluate the vigor of seeds classified by color and size. Ten sub-lots were obtained by separating the seeds into two tegument colors (light and dark) and five size classes (11, 12, 13, 14, and 15), using round wire sieves. Before the  accelerated aging (AA) test, the seeds were treated with concentrated sulfuric acid for 20 minutes to overcome dormancy. Next, the sub-lot seeds underwent different aging times (0, 24, 48, 72, 96 and 120 hours) at 45 ºC. The  AA test, conducted at 45 ºC for 72 hours, is adequate to evaluate the quality of P. moniliformis seeds, in terms of color and size.


Revista CERES ◽  
2015 ◽  
Vol 62 (5) ◽  
pp. 446-452 ◽  
Author(s):  
Vanessa Leonardo Ignácio ◽  
Ivair André Nava ◽  
Marlene de Matos Malavasi ◽  
Eloir Paulo Gris

ABSTRACTThis study aimed to evaluate the influence of foliar fertilizer doses containing Mn of phenological stages of suggested application in RR soybeans, to recover management damages with glyphosate at postemergence application on seed vigor in post-harvest and post six months storage. The seeds originated from a field experiment conducted , which included two applications of glyphosate, concomitant with foliar fertilizer in growth stages V4 and V6, with 0.00, 113.50 and 227.00 mg ha-1doses of Mn2+. Germination, GSI (Germination Speed Index), electrical conductivity tests and the first count of seeds were conducted. The application of Mn did not affect the physiological quality of RR soy in postharvest. However, in post-storage, higher doses of Mn had a negative effect on tests of abnormal seedlings, GSI and electrical conductivity. The applications of Mn, regardless of the developmental stage, did not interfere in the germination and first count tests, with and without storage. The electrical conductivity test showed a higher correlation with the seed germination test in the post-harvest treatment.


2018 ◽  
Vol 5 (4) ◽  
pp. 7-12
Author(s):  
Glauciana Da Mata Ataíde ◽  
Eduardo Euclydes de Lima Borges ◽  
Andressa Vasconcelos Flores

The electrical conductivity test is a simple and rapid method for evaluating seed vigor. The aim of the present study was to optimize the electrical conductivity test for assessing the vigor of Melanoxylon brauna seeds by investigating the effects of seed number (25, 50, or 75), water volume (25, 50, or 75 mL), and soaking duration (24, 48, or 72 h). The seeds belonged to two lots (I and II) that were collected in 2010 and 2012, respectively, and were incubated in a germination chamber at 25 ºC. Electrical conductivity was determined using a conductivity meter Micronal model B220. The Lot II seeds performed better, in terms of vigor and germination, than the Lot I seeds, regardless of other parameters. In addition, electrical conductivity decreased with increasing water volume and soaking duration, regardless of seed lot. In order to most effectively evaluate the physiological quality of M. brauna seed lots, the electrical conductivity test should be performed using 50 seeds, 50 mL of water, and a 48-h soaking period.


Sign in / Sign up

Export Citation Format

Share Document