scholarly journals Antibacterial Activity of a New Ready-To-Use Calcium Silicate-Based Sealer

2020 ◽  
Vol 31 (6) ◽  
pp. 611-616
Author(s):  
Vanessa Maia Barbosa ◽  
André Pitondo-Silva ◽  
Mariana Oliveira-Silva ◽  
Antônio Secco Martorano ◽  
Claudia de Castro Rizzi-Maia ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Calcium Silicate ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Ah Plus ◽  
Agar Diffusion Method ◽  
Imagej Software ◽  
Endodontic Sealers

Abstract The aim of this study was to evaluate the antibacterial potential of a calcium silicate-based sealer (Bio-C Sealer, Angelus) against common bacteria in primary and secondary endodontic infections. Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus mutans were exposed to fresh Bio-C Sealer for 24 h by the agar diffusion method (n=5). Additionally, the antibacterial activity was investigated against E. faecalis and S. mutans biofilms (48 h old) grown in discs with 4 mm in diameter and 2 mm in height. (n=3) of set discs of Bio-C Sealer (Angelus), EndoFill (Dentsply-Mallefer), Sealer 26 (Dentsply), AH Plus (Dentsply), Sealapex (Sybron-Endo) and EndoSequence BC Sealer (Brasseler). The antibacterial activity was evaluated by colony forming unity (CFU) counting using ImageJ software. Data were compared by one-way ANOVA followed by Holm-Sidak test (a=5%). Fresh Bio-C Sealer exhibited antimicrobial activity against all bacteria evaluated by agar diffusion method, except for S. mutans. Set discs of all endodontic sealers tested showed similar CFU values for E. faecalis (p>0.05). S. mutans in biofilms showed higher susceptibility to EndoFill compared with the other sealers (p<0.05). In conclusion, the results indicate that fresh Bio-C Sealer does not inhibit S. mutans growth, but exhibits antibacterial activity against E. faecalis, S. aureus, P. aeruginosa and E. coli. After setting, the Bio-C Sealer exhibits an antimicrobial potential comparable to that of the other sealers evaluated in E. faecalis biofilm, but lower than that of EndoFill for S. mutans biofilm.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals PERBANDINGAN PENGUJIAN AKTIVITAS ANTIBAKTERI STARTER YOGURT DENGAN METODE DIFUSI SUMURAN DAN METODE DIFUSI CAKRAM

2020 ◽  
Vol 1 (2) ◽  
pp. 41
Author(s):  
Lilih Siti Nurhayati ◽  
Nadhira Yahdiyani ◽  
Akhmad Hidayatulloh
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Activity ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Testing Methods ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Agar Well Diffusion Method

Antibacterial activity testing can be done using the agar diffusion method, including agar well difussion and disk diffusion agar methods. This study aims to compare two antibacterial testing methods to analyze the anti-bacterial activity of a yogurt starter against the bacteria Eschericia coli and Staphilococcus aureus. The study was conducted experimentally with 5 concentrations of yogurt starter, namely 2%, 4%, 6%, 8%, and 10%. Testing antibacterial activity using two methods ,disk diffusion agar and well difussion agar methods. The research showed that agar well diffusion method obtained antibacterial activity greater than the disk diffusion method for E. coli and S. aureus.

UJI AKTIVITAS ANTIBAKTERI BEBERAPA SPONS DARI PERAIRAN SALIBABU KEPULAUAN TALAUD

2019 ◽  
Vol 7 (3) ◽  
pp. 234
Author(s):  
Remus B Maradou ◽  
Fitje Losung ◽  
Remmy EP Msngindaan ◽  
Rosita AJ Lintang ◽  
Wilmy E Pelle ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Coral Reefs ◽  
Key Words ◽  
Crude Extract ◽  
Bioactive Compound ◽  
Diffusion Method ◽  
Water Fraction ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Sponge is one component of coral reefs that has the potential as a bioactive compound but has not been widely used. This study was directed to obtain some spongy crude extracts from the Salibabu waters of the Talaud Islands, determine the antibacterial activity of some crude extruded sponges and compare the antibacterial activity of the sponge fraction against E. coli and S. aureus test bacteria. The results of identification of sponges were found in three species consisting of: Siphonodictyon sp., Ircinia sp., Dysidea sp. The antibacterial activity of crude extract, water, methanol and hexane fractions using agar diffusion method with E. coli and S. aureus test bacteria. The crude extract of siphonodictyon sp and the water fraction of this extract show the highest antibacterial activity. Key words: Spons, Antibacterial, E. coli. S. aureus

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

Improved Agar Diffusion Method for Detecting Residual Antimicrobial Agents

2001 ◽  
Vol 64 (3) ◽  
pp. 361-366 ◽  
Author(s):  
CHIN-EN TSAI ◽  
FUSAO KONDO
Keyword(s):  
Antimicrobial Agents ◽  
Micrococcus Luteus ◽  
Oxolinic Acid ◽  
Photobacterium Phosphoreum ◽  
Penicillin G ◽  
Diffusion Method ◽  
E Coli ◽  
Assay Medium ◽  
Agar Diffusion ◽  
Agar Diffusion Method

The improved agar diffusion method for determination of residual antimicrobial agents was investigated, and the sensitivities of various combinations of test organisms and assay media were determined using 7 organisms, 5 media, and 31 antimicrobial agents. Bacillus stearothermophilus and synthetic assay medium (SAM) showed the greatest sensitivity for screening penicillins (penicillin G and ampicillin). The combination of Bacillus subtilis and minimum medium (MM) was the most sensitive for tetracyclines (oxytetracycline and chlortetracycline), B. stearothermophilus and SAM or Micrococcus luteus and Mueller-Hinton agar (MHA) for detecting tylosin and erythromycin, B. subtilis and MHA for aminoglycosides (streptomycin, kanamycin, gentamicin, and dihydrostreptomycin), B. stearothermophilus and SAM for polyethers (salinomycin and lasalocid), and B. subtilis and MM or Clostridium perfringens and GAM for polypeptides (thiopeptin, enramycin, virginiamycin, and bacitracin). However, gram-negative bacterium Escherichia coli ATCC 27166 and MM were better for screening for colistin and polymixin-B. For detecting the synthetic drugs tested, the best combination was B. subtilis and MM for sulfonamides, E. coli 27166 and MM for quinolones (oxolinic acid and nalidixic acid), B. subtilis and MM for furans (furazolidone), and the bioluminescent bacterium Photobacterium phosphoreum and luminescence assay medium for chloramphenicol and oxolinic acid. The results showed that the use of four assay plates, B. stearothermophilus and SAM, B. subtilis and MM, M. luteus and MHA, and E. coli 27166 and MM, was superior to the currently available techniques for screening for residual antimicrobial agents in edible animal tissues.

scholarly journals Synthesis, Characterization and Antibacterial Activity of Some Halo Substituted Schiff Bases

2010 ◽  
Vol 7 (3) ◽  
pp. 882-886
Author(s):  
S. B. Junne ◽  
Archana B. Kadam ◽  
S. L. Shinde ◽  
G. S. Waghamare ◽  
Y. B. Vibhute
Keyword(s):  
Antibacterial Activity ◽  
Schiff Bases ◽  
Aromatic Amines ◽  
Pathogenic Bacteria ◽  
Aromatic Aldehydes ◽  
Biologically Active ◽  
Diffusion Method ◽  
Active Compounds ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Some new halo substituted Schiff bases have been prepared from different aromatic aldehydes and a series of substituted aromatic amines to form a number of potentially biologically active compounds. The structures of the Schiff bases have been characterized by using IR and1HNMR spectroscopy. These compounds were screened against human pathogenic bacteria by agar diffusion method. Ampicillin was used as control.

scholarly journals In vitro evaluation of the antimicrobial activity of endodontic sealers

2006 ◽  
Vol 20 (4) ◽  
pp. 303-306 ◽  
Author(s):  
Daniela Cristina Miyagak ◽  
Elaine Manso Oliveira Franco de Carvalho ◽  
Carlos Roberto Colombo Robazza ◽  
Jorge Kleber Chavasco ◽  
Gustavo Labegalline Levorato
Keyword(s):  
Antimicrobial Activity ◽  
Portland Cement ◽  
Mineral Trioxide Aggregate ◽  
Diffusion Method ◽  
E Coli ◽  
Microbial Inhibition ◽  
Ah Plus ◽  
Inhibition Zones ◽  
Endodontic Sealers

The purpose of this study is to evaluate the antimicrobial activity of the endodontic sealers: N-Rickert, Sealapex, AH Plus, Mineral Trioxide Aggregate (MTA) and portland cement. The Agar diffusion method was used in plates previously inoculated with the following microorganisms: C. albicans, S. aureus, E. faecalis, E. coli. The diameters of microbial inhibition zones were measured after 24 hours of incubation in kiln at 37°C. According to the methodology used, it was possible to conclude that only the sealers AH Plus and N-Rickert presented antimicrobial activity against C. albicans, S. aureus, and E. coli; no antimicrobial activity in MTA, Sealapex and portland cement was observed. N-Rickert presented the largest inhibition zones varying from 8 to 18 mm, and the microorganism E. faecalis was resistant against all sealers tested.

scholarly journals Evaluation of Antimicrobial Activities of Crude Methanol Extract of Phoenix dactylifera Seeds on Clinical Isolates of Different Strains of E. coli

2019 ◽  
pp. 1-7
Author(s):  
C. Eze Chinelo ◽  
N. Ezeibe Ezinwanne ◽  
A. Ozioko Chizoba ◽  
C. Agbo Martina ◽  
N. Ugwu Chineye ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Phoenix Dactylifera ◽  
Antimicrobial Activities ◽  
Seed Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

Aim: This study was conducted to evaluate the antimicrobial activities of seed extract of Phoenix dactylifera. Study Design: Extraction of active ingredients of Phoenix dactylifera seeds using methanol and its effects on selected clinical isolates and isolated strains of E. coli. Place and Duration of Study: `Department of Phatrmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical, University of Nigeria, Nsukka between march 2017 and October 2018. Methodology: The seed of phoenix dactylifera were washed thoroughly to get rid of any adhering date flesh, air dried and ground. The ground powders were subjected to extraction by cold maceration using methanol. Preliminary sensitivity test was carried out against eight microorganisms, namely: Pseudomonas aeruginosa, E. coli, Enterococcus, Klebsiella, Staph. spp, Salmonella spp, Bacillus sp and Candida albicans using agar diffusion method. Antimicrobial susceptibility tests were carried out on isolated strains of E. coli from urine samples using agar diffusion method. The minimal inhibitory concentrations of crude methanol extract of seeds of Phoenix dactylifera were determined using agar diffusion method. The phytochemical analysis was conducted to determine the secondary metabolites. Results: The preliminary sensitivity test performed shows that the crude methanol extract of date seed were sensitive against all organisms tested. The active ingredients of the extract showed activities against all strains of E. coli tested though their zones of inhibition vary (18-21mm). The minimum inhibitory concentration (MIC) value ranges from 10-19.9 mg/ml. The phytochemical analysis of crude methanol seed extract of P. dacterifera indicated the presence of alkaloids, flavonoids, tannins, carbohydrates, proteins, reducing sugar, sterols and Terpenes, Anthraquinone glycosides. Conclusion: The present study provides the scientific information about Phoenix dactylifera seed activity.

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