scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

scholarly journals Antibacterial Activity Of Tangerine Powder (Citrus reticulata Blanco.) Against Caries-Causing Bacteria (Streptococcus mutans)

2020 ◽  
Vol 10 (2) ◽  
pp. 148-157
Author(s):  
Framesti Frisma Sriarumtias ◽  
Aji Najihudin ◽  
Nopi Rantika ◽  
Rita Nengsih
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Pharmaceutical Ingredients ◽  
Caries Treatment ◽  
Inhibitory Power

Dental caries is caused by Streptococcus mutans, which lives in the oral cavity and causes plaque on the teeth. Dental caries treatment involves restoration or filling of tooth tissue that has been perforated due to dental caries. The purpose of this study was analyze the effectivity of  tangerine juice powder in preventing the emergence of dental caries caused by Streptococcus mutans. The experimental method was used in this study started by making a powder from tangerine jiuce using freeze drying method, followed by testing the antibacterial activity using the disc diffusion method. In addition, the minimum inhibitory concentration (MIC) was also sought by measuring the diameter of the clear zone. The results showed that tangerine powder has antibacterial activity, with a minimum inhibitory concentration of 10%. The highest activity of tangerine powder to inhibit the growth of Streptococcus mutans bacteria was observed at   concentration of 25% with an inhibition zone diameter of 15.8 mm, and is classified as having moderate inhibitory power. According to the result of this study is that tangerine juice powder has a good antibacterial activity against Streptococcus mutans and can be developed into a pharmaceutical ingredients to prevent dental caries.

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

scholarly journals Assessment in Vitro of Antibacterial Activity of Manipulated Product, on Solution Form, Obtained from Dry Extract of Leaves of Psidium guajava L.

2015 ◽  
Vol 8 (1) ◽  
pp. 64
Author(s):  
Marcos V.S. Silva ◽  
Tayara S. Frazão ◽  
Melyssa M.A.V. Ferreira ◽  
Jandir S. Sales ◽  
Denis B. Vieira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Staphylococcus Epidermidis ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Dry Extract ◽  
Agar Diffusion ◽  
Agar Diffusion Method

<p class="Default">The <em>Psidium guajava L</em>. specie is a perennial shrub, belonging to the <em>Myrtaceae</em> family and it is popularly known as guava, its leaves are used in therapy for treating various diseases. The study aims to evaluate the antibacterial activity <em>in vitro </em>of manipulated product obtained from dried extract of the leaves of <em>P. guajava </em>L. front standard bacteria ATCC and clinical isolates. The tests were conducted on bacterial samples: <em>Staphylococcus aureus </em>(ATCC 25923), <em>Pseudomonas aeruginosa </em>(ATCC 27883), <em>Escherichia coli </em>(ATCC 25922), <em>Salmonella spp</em>, <em>Acinetobacter baumannii</em>, <em>Proteus mirabilis</em>, <em>Shigella flexneri</em>, <em>Staphylococcus epidermidis</em>, <em>Staphylococcus haemolyticus</em>, <em>Streptococcus agalactiae, Streptococcus mutans</em>. Among the tests can be mentioned phytochemical of the ethanol extract (EE), microbiological control and physical-chemical analysis of the product and microbiological tests such as agar diffusion method (wells), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), an evaluation test of hemolytic capacity of the solution and in vitro assay cytotoxic activity were performed, . The best result of the product in the agar diffusion method was front <em>Staphylococcus epidermidis</em>, while the lower MIC and MBC were front <em>Staphylococcus aureus </em>(ATCC 25923). The product showed no hemolytic activity and no cytotoxic activity at the tested concentrations. According to the test results, it is believed on the possibility of the production of a pharmaceutical formulation derived from the dry extract of <em>Psidium guajava</em>, since it showed great antibacterial activity.</p>

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

Uji Daya Hambat Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa blimbi L.) Terhadap Pertumbuhan Staphylococcus epidermidis

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Irma Zarwinda ◽  
Fauziah Fauziah ◽  
Shara Shevalinda ◽  
Dwi Putri Rejeki
Keyword(s):  
Staphylococcus Epidermidis ◽  
Research Method ◽  
Inhibitory Concentration ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Chemistry Laboratory ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Training And Education

Startfruit (Averrhoa bilimbi L.) is one the kind of plant that is widely used as a traditional herbal medicineto overcome various diseases i.e. diabetes mellitus, cough, rheumatism, thrush, diabetes, toothache, acne, etc. The secondary metabolites of the starfruit leaves contain flavonoids and tannins, whereby these activecompounds can be used as an antibacterial. This study aimed to determine the inhibiting power of starfruit leaf ethanol extract against Staphylococcus epidermidis at concentrations of 25%, 50%, 75%, and 100%. The research was conducted at the  AKAFARMA Laboratory and the Chemistry Laboratory , Faculty of Teacher Training and Education, Syiah Kuala University from June to July 2020. The research method was laboratory experimental using the disk diffusion method. The population of starfruit leaves was obtained from Lhokseumawe  using a purposive sampling technique. Ethanol extract of starfruit leaves with concentrations of 25%, 50%, 75%, and 100%. The diameter of the inhibition zone of the ethanol extract  from the starfruit leaves at  concentrations of 100%, 75%, 50%, and 25% were 15 mm, 12 mm, 11 mm and 10 mm, respectively. It can be concluded that  ethanol extract of starfruit leaves can inhibit the growth  of  Staphylococcus  epidermidis  with  a  maximum inhibitory concentration  of  100%,  which is classified  as a strong category.

scholarly journals STRUCTURE CHARACTERIZATION AND EVALUATION POTENTIAL OF ANTIMICROBIAL EXTRACTS FROM PHELLINUS LINTEUS AGAINST SKIN INFECTIOUS PATHOGENS, STAPHYLOCOCCUS EPIDERMIDIS ATCC12228 AND PROPIONIBACTERIUM ACNES DMST14916

2017 ◽  
Vol 9 (12) ◽  
pp. 78
Author(s):  
Surachai Techaoei ◽  
Pattaranut Eakwaropas ◽  
Khemjira Jarmkom ◽  
Warachate Khobjai
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Structure Characterization ◽  
Diffusion Method ◽  
Phellinus Linteus ◽  
Chemical Profile

Objective: The objective of this study was to investigate the antimicrobial activity of Phellinus linteus against skin infectious pathogens, Staphylococcus epidermidis ATCC12228 and Propionibacterium acnes DMST 14916.Methods: Fungal fruiting bodies were extracted with 95% ethanol and ethyl acetate, and then, vaporized. The antimicrobial activities were determined by the disc diffusion method against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 skin infectious pathogens. A minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) for those crude extracts were determined. Finally, the chemical profile of crude extract was determined by using thin layer chromatography and GC-MS.Results: The result demonstrated that the ethanolic extraction had more active fractions with an MIC of 0.5 mg/ml against the growth of Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 and also showed a minimum inhibitory concentration (MBC) at a concentration of 1.0 mg/ml, while ethyl acetate-based solvents failed to develop on TLC according to Retention factor (Rf) values of 0.71-0.76. The GC-MS was applied to investigate the chemical profile of crude extract of Phellinus linteus, revealing a component of hexadecanoic acid and 9, 12-octadecadienoic acid.Conclusion: Phellinus linteus fruiting body extracts have great potential as antimicrobial compounds against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228. Thus, they can be used in the treatment of infectious diseases caused by bacterial pathogens. 

scholarly journals Anti Acne Vulgaris Topical Cream From Combination Extract of Gymnanthemum Amygdalinum Del. and Elaeis Guineensis Jacq. Leaves

2020 ◽  
Vol 3 (1) ◽  
pp. 42-50
Author(s):  
Wahyudi ◽  
Ayu Syahfitri ◽  
Syukur Berkat Waruwu ◽  
Dian Ika Perbina
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Acne Vulgaris ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Propionibacterium Acne ◽  
Self Confidence ◽  
Adolescents And Adults

Acne is one problem in adolescents and adults who can reduce self-confidence. This study aimed to formulate the combination cream of African leaf extract and palm oil leaves to determine the antibacterial activity of African leaf extract against several acne-causing bacteria (Propionibacterium acne and Staphylococcus epidermidis). Test antibacterial activity of extracts and cream with disc diffusion method. The cream is made with concentration of 5, 10 and 20% w/w. The evaluation results of cream with concentration of 5, 10 and 20% were physically stable for 4 weeks of storage at temperature room, homogeneous, pH and it did not cause irritation. Based on the test results, a combination of African leaf extract with concentration of 5% effectively inhibited bacterial growth with an average inhibition zone diameter of 12.06 mm for Propionibacterium acne bacteria and 13.63 mm for Staphylococcus epidermidis bacteria. The result of this study suggest that combination of African leaf extract and palm leaves posses potent anti-bacterial activity against Propionibacterium acne and Staphylococcus epidermidis.

scholarly journals Investigation of Bulb Extracts of Crinum jagus for Antibacterial and Antifungal Activities

2021 ◽  
Vol 25 (1) ◽  
pp. 113-117
Author(s):  
T.T. Alawode ◽  
L. Lajide ◽  
B.J. Owolabi ◽  
M.T. Olaleye
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Antifungal Activities ◽  
Agar Diffusion ◽  
Antibacterial And Antifungal Activities ◽  
Successive Extraction ◽  
Antibacterial And Antifungal

This study investigates the bulb extracts of Crinum jagus for antimicrobial activities. The bulb samples were dried, ground and subjected to successive extraction using hexane, ethylacetate and methanol. The extracts were screened for activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebisidlae pneumonae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer at concentrations between 6.25 and 200 mg/ml. Antimicrobial assays werecarried out using agar diffusion method. The Minimum Inhibitory Concentration (MIC) of the extracts was determined. The percentage yields obtained for the hexane, ethylacetate and methanol extracts of the bulbs are 0.28 %, 0.44% and 24.68 % respectively. Results showed that the methanolic extract had better antibacterial and antifungal activities than the other extracts. The methanol extract showed the highest antibacterial activity against S. aureus with a zone of inhibition of 28 mm at 200 mg/ml. Also, the methanol extract showed the highest antifungal activities against C. albicans, A. niger and P. notatum with a zone of inhibition of 20 mm at 200 mg/ml. The hexane and ethylacetate extracts had no activity against the microorganisms at 6.25 mg/ml.The methanol extract had the lowest Minimum Inhibitory Concentration (MIC) of 2.50 mg/ml against S. aureus, E. coli, B. subtilis, P. aeruginosa and S. typhi. The methanol extract could be a source of potent antimicrobial compounds. Keywords: Antibacterial, Antifungal, Crinum jagus, Agar diffusion, MIC

scholarly journals ANTIBAKTERI SENYAWA FENOLIK DARI DAUN SAPUTANGAN (Maniltoa Grandiflora (A. Gray) Scheff)

2019 ◽  
Vol 1 (2) ◽  
pp. 17-21
Author(s):  
JHON PATAR SINURAT ◽  
SAADAH SIREGAR
Keyword(s):  
Phenolic Compound ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Inhibitory Zone ◽  
E Coli ◽  
Medium Level ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria ◽  
Positive Results

The Phytochemical screening test to identify secondary metabolites of Saputangan leaves extract (Maniltoa grandiflora (A.Gray) Scheff) showed positive results on phenolic and terpenoid compounds. The reagent used for phenolic screening was 5% FeCl3 and reagent for screening terpenoid was 1% CeSO4 in 10% H2SO4. Antibacterial test was carried out by agar diffusion method on extracts of DMSO Saputangan leaves. Phenolic compound obtained from extract of Saputangan leaves were 36.96 gram where the result was obtained after maceration, partitioning and evaporation processes. Antibacterial of phenolic compound was observed based on inhibitory zone diameters of phenolic compounds formed using paper discs with a diameter of 6 mm. The diameters of the inhibition zone against Escherichia coli bacteria are 8.1; 8.5 and 9.7 mm larger than the diameter of the inhibition zone in Staphylococcus aureus bacteria at 7.3; 7.8 and 8.5 mm. Then it can be stated that DMSO extracts of phenolic compound with concentrations (0.25; 0.50 and 1.00 mg / ml) have antibacterial strength at the medium level where the average inhibition zone of S. aureus bacteria is 7.87 mm and the average inhibition zone in E. coli bacteria is 8.76 mm.

Sign in / Sign up

Export Citation Format

Share Document