scholarly journals Butter whey and corn steep liquor as sole raw materials to obtain a bioemulsifier from Yarrowia lipolytica for food oil-in-water emulsions

2021 ◽  
Vol 51 (4) ◽  
Author(s):  
Fabiane Ferreira dos Santos ◽  
Karine Marques Lento de Freitas ◽  
Adejanildo da Silva Pereira ◽  
Gizele Cardoso Fontes-Sant’Ana ◽  
Maria Helena Miguez da Rocha-Leão ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Raw Materials ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Synthetic Medium ◽  
Production Costs ◽  
Emulsification Index ◽  
Oil In Water ◽  
Steep Liquor ◽  
Cheese Production

ABSTRACT: A synthetic medium containing glucose, glycerol, yeast extract (YE), and ammonium sulfate (AS) was compared to several low-cost media in their ability to produce high emulsification index (EI). The goal was to reduce the production costs of an emulsifier with application in food oil-in-water emulsions. To this end, agro-industrial by-products were screened for bioemulsifier production from Yarrowia lipolytica. The statistical analysis showed that the EIs of media containing residual frying oil from palm oil (RFO_palm) or soybean oil (RFO_soy), residual liquid from butter production (butter whey, BWhey) or cheese production (cheese whey, CWhey), supplemented with YE and AS were similar to the EI of the synthetic medium. The replacement of YE by corn steep liquor (CSL) also resulted in similar EI, except for RFO_soy. BWhey was tested with CSL without AS and similar EI (66.8%) was detected in comparison to that of the same medium with AS (66.3%). The cell-free broth obtained after Y. lipolytica growth in BWhey+CSL was successfully used to obtain vegetable oil-in-water emulsions indicating its potential application in food products.

scholarly journals Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

2020 ◽  
Vol 12 (17) ◽  
pp. 7122
Author(s):  
Ludwika Tomaszewska-Hetman ◽  
Waldemar Rymowicz ◽  
Anita Rywińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Culture Broth ◽  
Biodiesel Production ◽  
Innovative Strategy ◽  
Simple Medium ◽  
Low Cost Strategy ◽  
Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

scholarly journals Effects of corn steep liquor on β-poly(l-malic acid) production in Aureobasidium melanogenum

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Genan Wang ◽  
Bingyi Shi ◽  
Pan Zhang ◽  
Tingbin Zhao ◽  
Haisong Yin ◽  
...  
Keyword(s):  
Malic Acid ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Enrichment Analysis ◽  
Tca Cycle ◽  
Water Soluble ◽  
Growth And Yield ◽  
The Tca Cycle ◽  
Steep Liquor ◽  
Aureobasidium Melanogenum

Abstractβ-poly(l-malic acid) (PMLA) is a water-soluble biopolymer used in medicine, food, and other industries. However, the low level of PMLA biosynthesis in microorganisms limits its further application in the biotechnological industry. In this study, corn steep liquor (CSL), which processes high nutritional value and low-cost characteristics, was selected as a growth factor to increase the PMLA production in strain, Aureobasidium melanogenum, and its metabolomics change under the CSL addition was investigated. The results indicated that, with 3 g/L CSL, PMLA production, cell growth, and yield (Yp/x) were increased by 32.76%, 41.82%, and 47.43%, respectively. The intracellular metabolites of A. melanogenum, such as amino acids, organic acids, and key intermediates in the TCA cycle, increased after the addition of CSL, and the enrichment analysis showed that tyrosine may play a major role in the PMLA biosynthesis. The results presented in this study demonstrated that the addition of CSL would be an efficient approach to improve PMLA production.

scholarly journals Synthesis of the Microbial Polysaccharide Gellan from Dairy and Plant-Based Processing Coproducts

2021 ◽  
Vol 2 (2) ◽  
pp. 234-244
Author(s):  
Thomas P. West
Keyword(s):  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Culture Conditions ◽  
Water Soluble ◽  
Production Costs ◽  
Microbial Polysaccharide ◽  
Steep Liquor ◽  
The Cost ◽  
Condensed Distillers Solubles

This review examines the production of the microbial polysaccharide gellan, synthesized by Sphingomonas elodea, on dairy and plant-based processing coproducts. Gellan is a water-soluble gum that structurally exists as a tetrasaccharide comprised of 20% glucuronic acid, 60% glucose and 20% rhamnose, for which various food, non-food and biomedical applications have been reported. A number of carbon and nitrogen sources have been tested to determine whether they can support bacterial gellan production, with several studies attempting to optimize gellan production by varying the culture conditions. The genetics of the biosynthesis of gellan has been explored in a number of investigations and specific genes have been identified that encode the enzymes responsible for the synthesis of this polysaccharide. Genetic mutants exhibiting overproduction of gellan have also been identified and characterized. Several dairy and plant-based processing coproducts have been screened to learn whether they can support the production of gellan in an attempt to lower the cost of synthesizing the microbial polysaccharide. Of the processing coproducts explored, soluble starch as a carbon source supported the highest gellan production by S. elodea grown at 30 °C. The corn processing coproducts corn steep liquor or condensed distillers solubles appear to be effective nitrogen sources for gellan production. It was concluded that further research on producing gellan using a combination of processing coproducts could be an effective solution in lowering its overall production costs.

scholarly journals Eco-Friendly Bioemulsifier Production by Mucor circinelloides UCP0001 Isolated from Mangrove Sediments Using Renewable Substrates for Environmental Applications

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 365 ◽  
Author(s):  
Nathália S. A. A. Marques ◽  
Israel G. Sales da Silva ◽  
Davi L. Cavalcanti ◽  
Patrícia C. S. V. Maia ◽  
Vanessa P. Santos ◽  
...  
Keyword(s):  
Canola Oil ◽  
Raw Materials ◽  
Corn Steep Liquor ◽  
Mucor Circinelloides ◽  
Artemia Salina ◽  
Engine Oil ◽  
Extreme Conditions ◽  
Linear Quadratic ◽  
Statistical Validation ◽  
Steep Liquor

The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. In the present work, an economically attractive medium composed of corn steep liquor and waste cooking oil was formulated to maximize the production of bioemulsifier by Mucor circinelloides UCP0001. A central rotational composite design was applied to statistical validation of the production. The emulsifying properties, stability under extreme conditions, its toxicity character, and the characterization of the bioemulsifier were determined. The best condition for biomolecule synthesis occurred in the assay 2 containing 4% of corn steep liquor and 3% waste soybean oil and exhibited 100% emulsification index for canola oil and petroleum, as well as excellent emulsifying activity for canola oil and burned engine oil. The nutritional factors studied showed statistical relevance, since all linear, quadratic effects and their interactions were significant. The bioemulsifier showed 2.69 g/L yield and the chemical character of the molecule structure was identified by FT-IR (Fourier Transform Infrared) spectroscopy. The bioemulsifier showed no toxicity to Artemia salina and Chlorella vulgaris. Stable emulsions were obtained under extreme conditions of temperature, pH, and salinity. These findings contribute to understanding of the relationship between production, physical properties, chemical composition, and stability of bioemulsifier for their potential applications in biotechnology, such as bioremediation of hydrocarbon-contaminated soil and water.

scholarly journals Materials and Applications for Low-Cost Ceramic Membranes

Membranes ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 105 ◽  
Author(s):  
Amanmyrat Abdullayev ◽  
Maged Bekheet ◽  
Dorian Hanaor ◽  
Aleksander Gurlo
Keyword(s):  
Water Treatment ◽  
Raw Materials ◽  
Low Cost ◽  
Temperature Stability ◽  
Ceramic Membranes ◽  
Production Costs ◽  
High Temperature Stability ◽  
Waste Products ◽  
Recent Developments ◽  
Materials Used

In water treatment applications, the use of ceramic membranes is associated with numerous advantages relative to polymer-based filtration systems. High-temperature stability, fouling resistance, and low maintenance requirements contribute to lower lifecycle costs in such systems. However, the high production costs of most commercially available ceramic membranes, stemming from raw materials and processing, are uneconomical for such systems in most water treatment applications. For this reason, there is a growing demand for new ceramic membranes based on low-cost raw materials and processes. The use of unrefined mineral feedstocks, clays, cement, sands, and ash as the basis for the fabrication of ceramic membranes offers a promising pathway towards the obtainment of effective filtration systems that can be economically implemented in large volumes. The design of effective ceramic filtration membranes based on low-cost raw materials and energy-efficient processes requires a balance of pore structure, mass flow, and robustness, all of which are highly dependent on the composition of materials used, the inclusion of various pore-forming and binding additives, and the thermal treatments to which membranes are subjected. In this review, we present recent developments in materials and processes for the fabrication of low-cost membranes from unrefined raw materials, including clays, zeolites, apatite, waste products, including fly ash and rice husk ash, and cement. We examine multiple aspects of materials design and address the challenges relating to their further development.

scholarly journals Ecological-and-Economic Evaluation of Vinyl Chloride Production in Mineral Resource Clusters

2018 ◽  
Vol 41 ◽  
pp. 02025 ◽  
Author(s):  
Irina Kudryashova ◽  
Evgeniy Kharlampenkov ◽  
Natalia Zakharova ◽  
Anastasiya Kolevatova
Keyword(s):  
Energy Intensity ◽  
Raw Materials ◽  
Low Cost ◽  
Production Costs ◽  
Material Consumption ◽  
Chemical Company ◽  
The Past ◽  
Method Of Production ◽  
Production Capacities ◽  
Energy Companies

Over the past six years, the volume of plastics production inprimary forms in Russia increased by 57%, exceeding 7.8 million tons in 2017. During this period, the production capacities were substantially renovated; new plants were put into operation. Increasing the competitiveness of Russian polyvinyl chloride (PVC) is possible on the basis of the integrated use of raw materials, the use of innovative technologies and reduction of production costs. When calculating costs, the following aspects were taken into account: type, availability and cost of raw materials, the number of chemical stages of final product production, its yield, energy intensity and material consumption of the process, the possibility of combining this method of production with other productionp rocesses of a chemical company, i.e. complex use of all obtained products. This is due to the low cost of raw materials, the clustering of coaland chemical enterprises and energy companies located in the Kemerovo region, which makes the production of PVC competitive.

scholarly journals Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020 ◽  
Vol 8 (4) ◽  
pp. 574 ◽  
Author(s):  
Patrick Fickers ◽  
Hairong Cheng ◽  
Carol Sze Ki Lin
Keyword(s):  
Organic Acids ◽  
Yarrowia Lipolytica ◽  
Current Knowledge ◽  
Raw Materials ◽  
Low Cost ◽  
Industrial Wastes ◽  
Sugar Alcohols ◽  
Cell Factories ◽  
Main Challenge ◽  
Pharmaceutical Industries

Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

Extracellular constitutive production of pullulan-hydrolysing activity by Sclerotium rolfsii

1988 ◽  
Vol 34 (1) ◽  
pp. 82-85 ◽  
Author(s):  
Hemant S. Kelkar ◽  
Anil H. Lachke ◽  
Mukund V. Deshpande
Keyword(s):  
Inorganic Nitrogen ◽  
Corn Steep Liquor ◽  
Synthetic Medium ◽  
Carbon Sources ◽  
Sclerotium Rolfsii ◽  
Native Enzyme ◽  
Specific Viscosity ◽  
Constitutive Production ◽  
Ph Range ◽  
Steep Liquor

Extracellular constitutive pullulan-hydrolysing activity was detected in Sclerotium rolfsii (1.05 μmol glucose equivalents liberated∙mL−1∙min−1) when cultivated in a synthetic medium containing starch as the carbon source. The influence of various inorganic nitrogen and carbon sources, surfactants, and organic supplements on the production of pullulan-hydrolysing activity was studied. In an optimized medium supplemented with 1% corn steep liquor, increased yields of enzyme activity (1.8 μmol∙mL−1∙min−1) were obtained. The enzyme was most active at pH 4.2 and was stable in the pH range of 3.5–5.5. The optimum temperature for pullulan hydrolysis was 50 °C and the activity was stable in the temperature range of 25–60 °C at pH 4.2 for 30 min. The Ea for the native enzyme from the Arrhenius plot with pullulan as a substrate was 22.6 kJ∙mol−1. The Km value for the native enzyme with pullulan as a substrate was 8.33 mg∙mL−1. The enzyme did not produce glucose or maltose from pullulan. Inability of the enzyme to rapidly decrease the specific viscosity (ηsp) of a 1% pullulan solution indicated susceptibility of α-(1 → 6) and not α-(1 → 4) linkages.

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