scholarly journals Antioxidant activity and physiological performance of popcorn seed after saline stress and analysis of seedling images

2020 ◽  
Vol 44 ◽  
Author(s):  
Hugo Cesar Rodrigues Moreira Catão ◽  
Franciele Caixeta ◽  
Amanda Moreira Lopes ◽  
Flavia Andrea Nery-Silva ◽  
Adílio de Sá Júnior

ABSTRACT Salinity influences all germination stages and may adversely affect seedling establishment in the field. The objective of this research was to determine the relationship between the antioxidant activity and the physiological performance of seeds and to verify the efficacy of the imaging analysis of popcorn seedlings after seed exposure to saline stress. A completely randomized design was used that consisted of four replicates in a factorial scheme. This scheme comprised three popcorn hybrids (P618, AP6002, and AP8203) and five saline potential levels (0.0; -0.1; -0.3; -0.6, and -0.9 MPa) that were obtained from KCl solutions of different concentrations. First count germination, germination and seedling vigor classification (strong normal seedlings), and dry mass of seedlings were evaluated to determine the physiological quality of the seed. The images of seedlings were used to evaluate the coleoptile and root lengths, indices of vigor, uniformity, and growth using the Groundeye® software. The activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) were also determined. Saline stress compromises the physiological quality and antioxidant activity of seeds and the performance of popcorn seedlings. Computerized image analysis using Groundeye® was efficient in evaluating the popcorn seedlings after being subjected to salt stress. Hybrids AP6002 and AP8203 were more tolerant to salt stress than hybrid P618.

2018 ◽  
Vol 10 (7) ◽  
pp. 455
Author(s):  
Hozano De Souza Lemos Neto ◽  
Marcelo De Almeida Guimarães ◽  
Rosilene Oliveira Mesquita ◽  
Italo Marlone Gomes Sampaio ◽  
Ana Régia Alves de Araújo Hendges ◽  
...  

Silicon (Si) is a mineral element that provides many benefits to plants and may also assist in tolerance to abiotic stresses such as salinity. The aim of the study was to evaluate the use of Si as a possible attenuator of the deleterious effects of salinity on growth and post-harvest of lettuce. A completely randomized design with four replicates was performed in a 3 × 2 factorial, consisting of three salinity levels (1.65, 3.65, 7.65 dS m-1) and two levels of silicon (0.0 and 2.0 mM). Salinity reduced all variables related to plant growth. For shoot fresh and dry mass, it was observed that NaCl caused a reduction of 79.80 and 80%, respectively, in comparison to the control. However, parameters related to post-harvest such as titratable acidity, soluble solids and vitamin C increased with salinity. Although not effective in reducing saline stress on plant growth, Si provided improvements in the variables related to lettuce post-harvest. Salinity significantly reduced plant growth in the presence and absence of Si, but provided better post-harvest quality with Si.


2018 ◽  
Vol 31 (3) ◽  
pp. 651-656
Author(s):  
ERIC FABIANO SERAGUZI ◽  
CARLOS HENRIQUE QUEIROZ REGO ◽  
FERNANDA BRITO CARDOSO ◽  
ANA CARINA DA SILVA CÂNDIDO ◽  
CHARLINE ZARATIN ALVES

ABSTRACT This study aimed to evaluate the effects of chemical treatment of seeds with fungicide and insecticide on the seed physiological quality of Brachiaria brizantha cultivar MG5. Two experiments with four replicates were carried out in a completely randomized design. In the first experiment, the seeds were treated with the fungicide pyraclostrobin + fipronil + thiophanate-methyl and in the second, with the insecticide thiamethoxam, both at doses of 0, 150, 300, 450, and 600 mL of commercial product (CP)/100 kg of seeds. Physiological characterization was done on the basis of the first germination count, germination (%), emergence (%), emergence speed index, and length and dry mass of shoot and root. The treatment of B. brizantha seeds with the fungicide pyraclostrobin + fipronil + thiophanate-methyl benefits the physiological quality of seeds, improving germination and root development, with no phytotoxic effect up to the dose of 600 mL of CP/100 kg of seeds. The insecticide thiamethoxam has a biostimulating effect on B. brizantha cultivar MG5 up to the dose of 270 mL of CP/100 kg of seeds, but is phytotoxic in larger doses.


2017 ◽  
Vol 39 (3) ◽  
pp. 234-243 ◽  
Author(s):  
Hugo César Rodrigues Moreira Catão ◽  
Franciele Caixeta

Abstract: This present study aimed to assess seed quality of popcorn seedlings subjected to low temperatures by examining their physiological changes, enzymes, and images. We used a completely randomized design with four replications in a factorial arrangement with four hybrids (P618, AP6002, AP8202, AP8203) and five temperatures (10, 13, 16, 19 and 25 °C). Their physiological quality was assessed through radicle protrusion, germination, dry mass of seedlings and emergence. We have assessed the images of seedlings through their hypocotyl and root lengths; and through rates of automatic force, uniformity and growth, provided by Groundeye. The isozyme expressions were determined for the catalase (CAT - IUBMB: EC 1.11.1.6), alcohol dehydrogenase (ADH - IUBMB: EC 1.1.1.1), malate dehydrogenase (MDH - IUBMB: EC: 1.1.1.37) and α-amylase (α-AMY - IUBMB: EC 3.2.1.1). Results show that low temperatures cause a negative effect on seed physiological quality in enzyme expression of CAT, ADH, MDH and α-AMY and on the performance of seedlings. These alterations compromise seed quality. Hybrids AP6002 and AP8203 should be recommended for sowing at up to 16 °C. The Groundeye software was efficient in the analysis of popcorn seedlings and in for the assessment of seed quality when submitted to low temperatures.


2021 ◽  
Vol 45 ◽  
Author(s):  
Jackson Silva Nóbrega ◽  
Toshik Iarley da Silva ◽  
Ronimeire Torres da Silva ◽  
Maria de Fátima de Queiroz Lopes ◽  
Francisco Romário Andrade Figueiredo ◽  
...  

ABSTRACT Salinity is one of the problems that limit plant development, with the germination, growth, and initial establishment stages being the most sensitive to saline stress. Erythrina velutina, popularly known as “mulungu” in Brazil, is a species native to the Caatinga biome, which grows in conditions of soil rich in salts. The damage caused by salinity to the quality of its seeds and the use of techniques that mitigate such effects are little known. Thus, this work aimed to evaluate salicylic acid as a mitigator of saline stress in the physiological quality of mulungu seeds. The quality of two seed lots from two Brazilian states (L1: Mossoró, Rio Grande do Norte, and L2: Pentencostes, Ceará) was evaluated. A completely randomized design in a 2 (seed soaking with salicylic acid (SA) - distilled water and 1.0 mM SA) × 4 (electrical conductivity of irrigation water - ECw - 0.0, 3.0, 6.0 and 9.0 dS m-1) factorial scheme with four replications of 50 seeds each was used. Saline stress reduces the quality of mulungu seeds and seedlings. SA increases the vigor of the seeds, promoting improvements in germination under saline conditions.


2014 ◽  
Vol 36 (3) ◽  
pp. 312-317 ◽  
Author(s):  
Narjara Walessa Nogueira ◽  
Rômulo Magno Oliveira de Freitas ◽  
Salvador Barros Torres ◽  
Caio César Pereira Leal

The seed maturation process is genetically controlled and involves an arranged sequence of morphological and physiological changes extending from fertilization to its total independence from the mother-plant. These changes also include a set of preparatory phases for the germination process, which are characterized for the synthesis and accumulation of nutrient reserves. Thereby, this study was developed aiming at assessing development and physiological quality of cowpea seeds during maturation process. To this, the cowpea pods of cultivar BRS-Guariba were harvested from the tenth day after anthesis (DAA) until the twenty sixth DAA, with four days intervals. Immediately after each harvest, seeds were manually extracted from the pods and then subjected to the following determinations: moisture content, first count of germination, final germination percentage, length of shoots and roots, hypocotyl diameter, and seedling dry mass. The experiment was conducted in a completely randomized design, with five treatments (DAA), and four replications to each treatment. Results have shown that cowpea seeds have fairly fast physiological maturation, and that seeds harvested between 14 and 18 DAA have better vigor as well as higher germination rates; thus the harvest performed during this period does not cause damages to seeds.


Author(s):  
Francisco E. C. B. Pereira ◽  
Sebastião Medeiros Filho ◽  
Salvador B. Torres ◽  
Cibele C. Martins ◽  
Selma F. de Brito

ABSTRACT The knowledge concerning the physiology of germination in saline areas may contribute to the development of more efficient cultural practices and adequate choice of planting areas. Thus, the objective of this study was to evaluate the effects of saline and temperature stress on germination and vigor of Piptadenia moniliformis (a species known in Brazil as "catanduva") seeds. The treatments were distributed according to a completely randomized design in accordance with a 3 x 6 factorial arrangement (temperature x osmotic potential), with four replicates of 25 seeds each. The seeds were put to germinate at constant temperatures of 25, 30, and 35 °C on paper towel moistened in distilled water (0.0) and NaCl solutions at 0.2, 0.4, 0.6, 0.8 and 1.0 MPa. The results were evaluated in terms of germination percentage and germination speed index, seedling (root and shoot) length, and total dry matter. P. moniliformis seeds were able to germinate under temperatures of 25 and 30 °C and tolerate osmotic potentials of up to -0.6 MPa; from this point on, there is progressive decrease in the physiological quality of seedlings.


2019 ◽  
Vol 11 (2) ◽  
pp. 541 ◽  
Author(s):  
Lanes B. A. Jaques ◽  
Ivan R. Carvalho ◽  
Vinícius J. Szareski ◽  
João R. Pimentel ◽  
Cristian Troyjack ◽  
...  

The growth regulators from gibberellins, when applied on plants, might improve physic and physiological features, stimulate cell division and elongation, this study aimed at evaluating the influence of gibberellic acid doses applied exogenously on bean crop through seed treatment, and analyzing its effects on morphological attributes of seedlings and physiological quality of the seeds produced. The experimental design was completely randomized design, with treatments corresponding to the doses: zero (distilled water only), 50, 100 and 200 mg L-1, arranged in four replicates. The use of gibberellic acid in bean seeds did not result in changes on the number of leaves, leaf area, dry mass of leaves and stem. Applications of foliar gibberellic acid potentiated stem height and root dry mass of seedlings.


2017 ◽  
Vol 39 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Jaciara de Souza Bispo ◽  
Danielle Carolina Campos da Costa ◽  
Samara Elizabeth Vieira Gomes ◽  
Gilmara Moreira de Oliveira ◽  
Janete Rodrigues Matias ◽  
...  

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.


2019 ◽  
Vol 41 (3) ◽  
pp. 336-343
Author(s):  
Everson Reis Carvalho ◽  
Victor Moss Francischini ◽  
Suemar Alexandre Gonçalves Avelar ◽  
Júlia Camargos da Costa

Abstract: Seeds harvested on the ears have high moisture content. On that account, this study aimed at evaluating the loss of physiological quality of corn seeds harvested on the ears, as a function of different drying-delay times. Hybrid corn ears were harvested at 31% moisture and then had their drying postponed for 0, 12, 24 and 36 h, while subjected to temperatures of 30, 40, 50 and 60 °C. The physiological quality was evaluated after 0, 4, 8 and 12 months of storage. A completely randomized design was employed, in a 4 x 4 x 4 factorial scheme, with four replications. In addition, a study was performed in a seed-processing unit, reporting the average waiting time before drying and the temperatures of all loads of a corn hybrid received at the facility. The physiological quality was not affected by temperatures below 40 °C, considering 36 h of waiting before drying. At 50 ºC during the drying delay, the germination was impaired 36 h afterward, and the vigor was compromised after 24 h, with the damage effects intensifying as the storage advanced. At the temperature of 60 ºC, the deficits in germination and vigor occurred within the first hours of drying delay.


2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.


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