Gibberellic Acid Utilization in Seeds and Plants of Beans: Effect on Growth and Seeds Physiological Quality

The growth regulators from gibberellins, when applied on plants, might improve physic and physiological features, stimulate cell division and elongation, this study aimed at evaluating the influence of gibberellic acid doses applied exogenously on bean crop through seed treatment, and analyzing its effects on morphological attributes of seedlings and physiological quality of the seeds produced. The experimental design was completely randomized design, with treatments corresponding to the doses: zero (distilled water only), 50, 100 and 200 mg L-1, arranged in four replicates. The use of gibberellic acid in bean seeds did not result in changes on the number of leaves, leaf area, dry mass of leaves and stem. Applications of foliar gibberellic acid potentiated stem height and root dry mass of seedlings.

Download Full-text

PHYSIOLOGICAL QUALITY OF Brachiaria brizantha SEEDS TREATED WITH FUNGICIDE AND INSECTICIDE

Revista Caatinga ◽

10.1590/1983-21252018v31n313rc ◽

2018 ◽

Vol 31 (3) ◽

pp. 651-656

Author(s):

ERIC FABIANO SERAGUZI ◽

CARLOS HENRIQUE QUEIROZ REGO ◽

FERNANDA BRITO CARDOSO ◽

ANA CARINA DA SILVA CÂNDIDO ◽

CHARLINE ZARATIN ALVES

Keyword(s):

Root Development ◽

Dry Mass ◽

Brachiaria Brizantha ◽

Thiophanate Methyl ◽

Physiological Characterization ◽

Randomized Design ◽

Phytotoxic Effect ◽

Physiological Quality ◽

Completely Randomized Design

ABSTRACT This study aimed to evaluate the effects of chemical treatment of seeds with fungicide and insecticide on the seed physiological quality of Brachiaria brizantha cultivar MG5. Two experiments with four replicates were carried out in a completely randomized design. In the first experiment, the seeds were treated with the fungicide pyraclostrobin + fipronil + thiophanate-methyl and in the second, with the insecticide thiamethoxam, both at doses of 0, 150, 300, 450, and 600 mL of commercial product (CP)/100 kg of seeds. Physiological characterization was done on the basis of the first germination count, germination (%), emergence (%), emergence speed index, and length and dry mass of shoot and root. The treatment of B. brizantha seeds with the fungicide pyraclostrobin + fipronil + thiophanate-methyl benefits the physiological quality of seeds, improving germination and root development, with no phytotoxic effect up to the dose of 600 mL of CP/100 kg of seeds. The insecticide thiamethoxam has a biostimulating effect on B. brizantha cultivar MG5 up to the dose of 270 mL of CP/100 kg of seeds, but is phytotoxic in larger doses.

Download Full-text

Antioxidant activity and physiological performance of popcorn seed after saline stress and analysis of seedling images

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044005020 ◽

2020 ◽

Vol 44 ◽

Author(s):

Hugo Cesar Rodrigues Moreira Catão ◽

Franciele Caixeta ◽

Amanda Moreira Lopes ◽

Flavia Andrea Nery-Silva ◽

Adílio de Sá Júnior

Keyword(s):

Antioxidant Activity ◽

Salt Stress ◽

Dry Mass ◽

Saline Stress ◽

Physiological Performance ◽

Randomized Design ◽

Physiological Quality ◽

Completely Randomized Design ◽

The Relationship

ABSTRACT Salinity influences all germination stages and may adversely affect seedling establishment in the field. The objective of this research was to determine the relationship between the antioxidant activity and the physiological performance of seeds and to verify the efficacy of the imaging analysis of popcorn seedlings after seed exposure to saline stress. A completely randomized design was used that consisted of four replicates in a factorial scheme. This scheme comprised three popcorn hybrids (P618, AP6002, and AP8203) and five saline potential levels (0.0; -0.1; -0.3; -0.6, and -0.9 MPa) that were obtained from KCl solutions of different concentrations. First count germination, germination and seedling vigor classification (strong normal seedlings), and dry mass of seedlings were evaluated to determine the physiological quality of the seed. The images of seedlings were used to evaluate the coleoptile and root lengths, indices of vigor, uniformity, and growth using the Groundeye® software. The activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) were also determined. Saline stress compromises the physiological quality and antioxidant activity of seeds and the performance of popcorn seedlings. Computerized image analysis using Groundeye® was efficient in evaluating the popcorn seedlings after being subjected to salt stress. Hybrids AP6002 and AP8203 were more tolerant to salt stress than hybrid P618.

Download Full-text

Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

Journal of Seed Science ◽

10.1590/2317-1545v39n3169853 ◽

2017 ◽

Vol 39 (3) ◽

pp. 234-243 ◽

Cited By ~ 3

Author(s):

Hugo César Rodrigues Moreira Catão ◽

Franciele Caixeta

Keyword(s):

Low Temperatures ◽

Seed Quality ◽

Dry Mass ◽

Enzyme Expression ◽

Randomized Design ◽

Radicle Protrusion ◽

Negative Effect ◽

Physiological Quality ◽

Completely Randomized Design

Abstract: This present study aimed to assess seed quality of popcorn seedlings subjected to low temperatures by examining their physiological changes, enzymes, and images. We used a completely randomized design with four replications in a factorial arrangement with four hybrids (P618, AP6002, AP8202, AP8203) and five temperatures (10, 13, 16, 19 and 25 °C). Their physiological quality was assessed through radicle protrusion, germination, dry mass of seedlings and emergence. We have assessed the images of seedlings through their hypocotyl and root lengths; and through rates of automatic force, uniformity and growth, provided by Groundeye. The isozyme expressions were determined for the catalase (CAT - IUBMB: EC 1.11.1.6), alcohol dehydrogenase (ADH - IUBMB: EC 1.1.1.1), malate dehydrogenase (MDH - IUBMB: EC: 1.1.1.37) and α-amylase (α-AMY - IUBMB: EC 3.2.1.1). Results show that low temperatures cause a negative effect on seed physiological quality in enzyme expression of CAT, ADH, MDH and α-AMY and on the performance of seedlings. These alterations compromise seed quality. Hybrids AP6002 and AP8203 should be recommended for sowing at up to 16 °C. The Groundeye software was efficient in the analysis of popcorn seedlings and in for the assessment of seed quality when submitted to low temperatures.

Download Full-text

Treatment with insecticide and fungicide on the physiological quality of lentil seeds

Brazilian Journal of Biology ◽

10.1590/1519-6984.246670 ◽

2023 ◽

Vol 83 ◽

Author(s):

J. Rubert ◽

S. H. B. Dornelles ◽

U. R. Nunes ◽

N. T. Pedrollo ◽

M. Peripolli ◽

...

Keyword(s):

Seed Treatment ◽

Accelerated Aging ◽

Lower Number ◽

Thiophanate Methyl ◽

Pests And Diseases ◽

Randomized Design ◽

Physiological Properties ◽

Physiological Quality ◽

Completely Randomized Design

Abstract The low Brazilian productive index and the high demand have aroused interest in the cultivation of lentils, however the legume is little known and needs further studies. The objective of this study was to analyze and identify the effects of treatments with insecticides and fungicides on the physiological quality of lentil seeds, CA-1512 strain. The experiments were conducted in the seed laboratory in a completely randomized design with seven treatments and four replicates. Seed treatment with Thiophanate-methyl; Fluazinam® (180 ml) + Pyraclostrobin; Thiophanate-methyl; Fipronil® (150 ml) promoted higher levels of germination under accelerated aging, lower number of abnormal seedlings and longer lengths of shoot and radicle for the emergence in paper. Treatment with Carboxin; Thiram® (250 ml) + Imidacloprid® (150 ml) allowed a higher value in the first count of germination in sand, lower number of dead seeds under accelerated aging and longer root length, in the emergence in sand. Shoot length in the emergence in sand increased after seed treatment with Metalaxyl-M; Fludioxonil® (75 ml) + Pyraclostrobin; Thiophanate-methyl; Fipronil® (150 ml). Treatments with fungicides and insecticides considerably improved the physiological properties of the seeds, thus being able to guarantee greater phytosanitary qualities in the field, generating healthier seedlings and with protection against possible pests and diseases, and consequently guaranteeing greater productivity.

Download Full-text

Physiological maturation of cowpea seeds

Journal of Seed Science ◽

10.1590/2317-1545v36n31007 ◽

2014 ◽

Vol 36 (3) ◽

pp. 312-317 ◽

Cited By ~ 3

Author(s):

Narjara Walessa Nogueira ◽

Rômulo Magno Oliveira de Freitas ◽

Salvador Barros Torres ◽

Caio César Pereira Leal

Keyword(s):

Dry Mass ◽

Germination Percentage ◽

Maturation Process ◽

Cowpea Seeds ◽

Randomized Design ◽

Germination Process ◽

Physiological Quality ◽

Completely Randomized Design ◽

Nutrient Reserves

The seed maturation process is genetically controlled and involves an arranged sequence of morphological and physiological changes extending from fertilization to its total independence from the mother-plant. These changes also include a set of preparatory phases for the germination process, which are characterized for the synthesis and accumulation of nutrient reserves. Thereby, this study was developed aiming at assessing development and physiological quality of cowpea seeds during maturation process. To this, the cowpea pods of cultivar BRS-Guariba were harvested from the tenth day after anthesis (DAA) until the twenty sixth DAA, with four days intervals. Immediately after each harvest, seeds were manually extracted from the pods and then subjected to the following determinations: moisture content, first count of germination, final germination percentage, length of shoots and roots, hypocotyl diameter, and seedling dry mass. The experiment was conducted in a completely randomized design, with five treatments (DAA), and four replications to each treatment. Results have shown that cowpea seeds have fairly fast physiological maturation, and that seeds harvested between 14 and 18 DAA have better vigor as well as higher germination rates; thus the harvest performed during this period does not cause damages to seeds.

Download Full-text

Seed and seedling quality of three chilis (Capsicum annuum L.) native to Oaxaca, Mexico

Ciência Rural ◽

10.1590/0103-8478cr20190921 ◽

2020 ◽

Vol 50 (9) ◽

Author(s):

Jair Sanjuan-Martínez ◽

Yolanda Donají Ortiz-Hernández ◽

Teodulfo Aquino-Bolaños ◽

Serafín Cruz-Izquierdo

Keyword(s):

Laboratory Experiments ◽

Germination Rate ◽

Green Pepper ◽

Randomized Design ◽

Vigor Index ◽

Physiological Quality ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Traditional Manner

ABSTRACT: The diversity of chilis in Oaxaca is one of the most extensive in Mexico. Growers select seeds in the traditional manner; information on its quality is unknown. The aim of this research was to compare the physical and physiological quality of seeds and seedlings of three native types to chili (Huacle, De agua and Pasilla) and of the commercial Green pepper as control. Fifteen variables were evaluated in seed and twelve in seedlings. The laboratory experiments were established under a completely randomized design. Means of the variables were compared using the Tukey test (α=0.05). Results showed that Pasilla chili had the highest values in seed size and weight, which was 1.44 g heavier than the control and 2.09 to 3.73 g higher than native types. Haucle chili and green pepper had a 90% germination rate, while chili De agua and Pasilla chili had germination rates of 51 and 38%, respectively. Huacle chili had the highest germination rate index (29.89) and seedling vigor index (992.25). The top highest values of number of normal seedlings were obtained in the control and Huacle chili seedlings (72 and 88%); also, the latter had the longest plumule (3.36 cm) and radicle (7.67 cm) values. Both Huacle and Pasilla chili seedlings had larger values in height, stem thickness, number of leaves, leaf area and slenderness index than De agua and Green pepper. Huacle chili had good physical and physiological quality similar to the seeds of the commercial Green pepper.

Download Full-text

Size and vigor of Anadenanthera colubrina (Vell.) Brenan seeds harvested in Caatinga areas

Journal of Seed Science ◽

10.1590/2317-1545v39n4173727 ◽

2017 ◽

Vol 39 (4) ◽

pp. 363-373 ◽

Cited By ~ 4

Author(s):

Jaciara de Souza Bispo ◽

Danielle Carolina Campos da Costa ◽

Samara Elizabeth Vieira Gomes ◽

Gilmara Moreira de Oliveira ◽

Janete Rodrigues Matias ◽

...

Keyword(s):

Electrical Conductivity ◽

Dry Matter ◽

Folk Medicine ◽

Germination Percentage ◽

Climate Variations ◽

Randomized Design ◽

Mother Plants ◽

Physiological Quality ◽

Completely Randomized Design

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.

Download Full-text

Application of Trichoderma harzianum on the physiological quality of cowpea seeds

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.05.p2865 ◽

2021 ◽

pp. 643-648

Author(s):

Marcelo Laranjeira Pimentel ◽

Henara Valéria Miranda Castro ◽

Mike Kirixi Munduruku ◽

Larissa Conceição Cunha Ponte ◽

Deyvielen Maria Ramos Alves ◽

...

Keyword(s):

Trichoderma Harzianum ◽

Seed Treatment ◽

Germination Percentage ◽

Hypocotyl Length ◽

Cowpea Seeds ◽

Randomized Design ◽

Germination Speed ◽

Physiological Quality ◽

Set Up

The use of fungi in seed treatment can improve the plant's physiological characteristics. The aim of this study was to evaluate the influence of the use of Trichoderma harzianum on the physiological quality of bean seeds. The experiment was set up and conducted in the forest seed laboratory of the Federal University of Western Para, in a randomized design, using 4 procedures on the basis of a colony forming unit (CFU): T1 (0 CFU), T2 (4 x 109 CFU), T3 (8 x 109 CFU) and T4 (12 x 109 CFU), with 5 repetitions. Germination percentage (G%), germination speed index (GSI), hypocotyl length (HL) and radicle length (RL) were evaluated as variables. The data were analyzed by variance analysis and the averages were compared using the Tukey test (p≤0.05), in addition to the regression analysis, using the Minitab© version 18 statistical software. There were differences between the germination percentage tests, in which T2 obtained 100% of germinated seeds, for GSI it was observed that T2 and T3 were equal and superior to the other treatments, while for RL T1 and T2 obtained the best results, however, for HL there were no statistical differences between treatments. T2 was more efficient in the physiological quality of seeds to germination percentage and germination speed index

Download Full-text

Temperatures and periods of drying delay and quality of corn seeds harvested on the ears

Journal of Seed Science ◽

10.1590/2317-1545v41n3218758 ◽

2019 ◽

Vol 41 (3) ◽

pp. 336-343

Author(s):

Everson Reis Carvalho ◽

Victor Moss Francischini ◽

Suemar Alexandre Gonçalves Avelar ◽

Júlia Camargos da Costa

Keyword(s):

Processing Unit ◽

High Moisture Content ◽

High Moisture ◽

Seed Processing ◽

Randomized Design ◽

Delay Times ◽

Physiological Quality ◽

Completely Randomized Design ◽

Corn Hybrid

Abstract: Seeds harvested on the ears have high moisture content. On that account, this study aimed at evaluating the loss of physiological quality of corn seeds harvested on the ears, as a function of different drying-delay times. Hybrid corn ears were harvested at 31% moisture and then had their drying postponed for 0, 12, 24 and 36 h, while subjected to temperatures of 30, 40, 50 and 60 °C. The physiological quality was evaluated after 0, 4, 8 and 12 months of storage. A completely randomized design was employed, in a 4 x 4 x 4 factorial scheme, with four replications. In addition, a study was performed in a seed-processing unit, reporting the average waiting time before drying and the temperatures of all loads of a corn hybrid received at the facility. The physiological quality was not affected by temperatures below 40 °C, considering 36 h of waiting before drying. At 50 ºC during the drying delay, the germination was impaired 36 h afterward, and the vigor was compromised after 24 h, with the damage effects intensifying as the storage advanced. At the temperature of 60 ºC, the deficits in germination and vigor occurred within the first hours of drying delay.

Download Full-text

Influence of Post Shooting Spray and Bunch Bagging on Per Day Productivity and Fruit Quality of Banana (Musa paradisiaca L.)

Journal of Scientific Research and Reports ◽

10.9734/jsrr/2021/v27i1130464 ◽

2021 ◽

pp. 129-136

Author(s):

D. R. Paradva ◽

M. J. Patel ◽

H. L. Kacha

Keyword(s):

Humic Acid ◽

Gibberellic Acid ◽

Shelf Life ◽

Blue Colour ◽

College Of Agriculture ◽

Musa Paradisiaca ◽

Randomized Design ◽

Completely Randomized Design ◽

Horticultural Research

Aims: To determine the effect of post shooting spray and bunch bagging on per day productivity and quality of banana (Musa paradisiaca L.)” Study Design: Completely Randomized Design (Factorial) with three repetitions. Place and Duration of Study: Experiment was carried out at the Horticultural Research Farm, Department of Horticulture, B. A. College of Agriculture, AAU, Anand during the year 2017-18 and 2018-19 Methodology: The experiment comprises of twenty four treatment combinations involving two varieties viz. Grand Naine and William with six levels of post shooting sprays namely; control, humic acid 2 %, 2, 4-D 30 mg/l, gibberellic acid (GA3) 100 mg/l, CPPU 4 mg/l and sulphate of potash (SOP) 2 % with two bunch bagging viz., non- woven material bag covering and blue colour polyethylene sleeve (6 % perforated) bag covering. Post shooting sprays were given twice i.e. 1st spray after complete opening of inflorescence and 2nd spray after 30 days of first spray with covering the bunch immediately after second spray. Results: The results indicated that the Grand Naine variety recorded significantly minimum harvest days, maturity days. Whereas, William variety was recorded significantly shelf life and fruit appearances. In case of per day productivity is concerned, both the varieties were equally important. The post shooting spray of GA3 100 mg/l recorded significantly improves per day productivity and fruit appearances. Whereas, post shooting spray of SOP 2 % recorded minimum harvest day and maturity days. While, post shooting sprays of CPPU 4 mg/l showed significantly maximum shelf life. The non-woven material bag covering was significantly better among all quantitative and qualitative parameters as compared to blue colour polyethylene sleeve bag covering. While, post shooting spray of CPPU 4 mg/l with non-woven material bag covering showed significantly expand the shelf life of fruit. Conclusion: Grand Naine variety recorded significantly minimum harvest and maturity days. Whereas, William variety was recorded significantly shelf life and fruit appearances. The post shooting spray of GA3 100 mg/l was improved per day productivity and fruit appearances. Whereas, post shooting sprays of CPPU 4 mg/l showed significantly enhancing the shelf life. The non-woven material bag covering on banana bunches was found significantly better among all quantitative and qualitative parameters.

Download Full-text