Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

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Seedling length and soybean seed vigor

Ciência Rural ◽

10.1590/0103-8478cr20190495 ◽

2021 ◽

Vol 51 (7) ◽

Author(s):

Michele Renata Revers Meneguzzo ◽

Géri Eduardo Meneghello ◽

Ariele Paula Nadal ◽

Fernanda da Mota Xavier ◽

Simone Morgan Dellagostin ◽

...

Keyword(s):

Seedling Growth ◽

Soybean Seed ◽

Dry Mass ◽

Seed Vigor ◽

Time Interval ◽

Soybean Seedling ◽

Randomized Design ◽

Seedling Length ◽

Completely Randomized Design ◽

The Moment

ABSTRACT: The differentiation of seedling growth and development is the result of the vigor level of the seed lot. Because of this, knowing the seedling growth curve is essential for the evaluation of the seedling length test, to be carried out at the moment when any differences in vigor really manifest, thus being able to reduce the time of evaluation of the test. In this research, the objective was to determine the most efficient time interval to perform the seedling length test in soybean seeds, depending on the vigor levels. The evaluated characteristics were percentage of seed germination, first germination count, emergency, days for emergence, aerial, root and total length (evaluated every 12 hours), aerial, root and total dry mass in completely randomized design. High vigor seeds originated seedlings with higher growth rates (cm. h-1), with vigor being one of the factors that interfere in the seedling length test. After 96 hours of the seedling length test, there was a statistical differentiation between the three vigor levels tested. The soybean seedling length test between lots, with different vigor, can be performed 96 hours after sowing.

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Physiological maturation of cowpea seeds

Journal of Seed Science ◽

10.1590/2317-1545v36n31007 ◽

2014 ◽

Vol 36 (3) ◽

pp. 312-317 ◽

Cited By ~ 3

Author(s):

Narjara Walessa Nogueira ◽

Rômulo Magno Oliveira de Freitas ◽

Salvador Barros Torres ◽

Caio César Pereira Leal

Keyword(s):

Dry Mass ◽

Germination Percentage ◽

Maturation Process ◽

Cowpea Seeds ◽

Randomized Design ◽

Germination Process ◽

Physiological Quality ◽

Completely Randomized Design ◽

Nutrient Reserves

The seed maturation process is genetically controlled and involves an arranged sequence of morphological and physiological changes extending from fertilization to its total independence from the mother-plant. These changes also include a set of preparatory phases for the germination process, which are characterized for the synthesis and accumulation of nutrient reserves. Thereby, this study was developed aiming at assessing development and physiological quality of cowpea seeds during maturation process. To this, the cowpea pods of cultivar BRS-Guariba were harvested from the tenth day after anthesis (DAA) until the twenty sixth DAA, with four days intervals. Immediately after each harvest, seeds were manually extracted from the pods and then subjected to the following determinations: moisture content, first count of germination, final germination percentage, length of shoots and roots, hypocotyl diameter, and seedling dry mass. The experiment was conducted in a completely randomized design, with five treatments (DAA), and four replications to each treatment. Results have shown that cowpea seeds have fairly fast physiological maturation, and that seeds harvested between 14 and 18 DAA have better vigor as well as higher germination rates; thus the harvest performed during this period does not cause damages to seeds.

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Aspectos germinativos de cultivares de girassol armazenadas submetidos a estresse térmico

Revista Verde de Agroecologia e Desenvolvimento Sustentável ◽

10.18378/rvads.v10i3.3315 ◽

2015 ◽

Vol 10 (3) ◽

pp. 95

Author(s):

Renato Costa Nunes Leandro ◽

Alexandre Bosco de Oliveira ◽

Tatiana Maria da Silva ◽

André Luís da Silva Parente Nogueira ◽

Maria Neurilan Costa Silva

Keyword(s):

Heat Stress ◽

Germination Percentage ◽

Sunflower Seeds ◽

Randomized Design ◽

Temperature Regimes ◽

Seed Analysis ◽

Physiological Quality ◽

Analysis Laboratory ◽

Completely Randomized Design ◽

The One

Objetivou-se avaliar a germinação de sementes de diferentes cultivares de girassol armazenadas sob condições de estresse térmico. O trabalho foi desenvolvido no Laboratório de Análise de Sementes do Departamento de Fitotecnia da Universidade Federal do Ceará (UFC), em Fortaleza - CE. Para a avaliação da influência da temperatura o experimento foi disposto em delineamento inteiramente casualizado em esquema fatorial (20 x 5), sendo vinte cultivares de girassol e cinco regimes de temperaturas (25,0; 27,5; 30,0; 32,5 e 35,0º C) e, respectivamente, conduzido quatro repetições. Foram avaliadas as seguintes variáveis: média da matéria fresca por plântula; média da matéria seca por plântula tempo médio de germinação; índice de velocidade de germinação; porcentagem de germinação. Os resultados foram comparados entre si pelo teste de Skott-Knott a 5% de probabilidade. A avaliação de estresse térmico promovido nas sementes de girassol, permitiram discriminar o híbrido Olissun como o que apresentou as melhores características em termos de qualidade fisiológica para superação de estresse térmico em comparação aos demais cultivares avaliados. Germinal aspects of stored sunflower cultivars subjected to heat stressAbstract: This study aimed to evaluate the germination of seeds of different cultivars of sunflower under conditions of heat stress. The study was conducted in the Plant Science Department of the Seed Analysis Laboratory of the Federal University of Ceará (UFC) in Fortaleza-CE. For the evaluation of the influence of temperature, the experiment was arranged in a completely randomized design in a factorial design (20 x 5), and twenty varieties of sunflower five temperature regimes (25.0, 27.5, 30.0, 32.5 and 35,0º C) and, respectively, conducted four replications. The following variables were evaluated: mean fresh weight per plant; average dry matter per plant average time of germination; germination speed index; germination percentage. The results were compared by Skott-Knott test at 5% probability. The evaluation of thermal stress caused in sunflower seeds, allowed to discriminate the hybrid Olissun as the one with the best characteristics in terms of physiological quality to overcome heat stress compared to other cultivars evaluated.

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Size and vigor of Anadenanthera colubrina (Vell.) Brenan seeds harvested in Caatinga areas

Journal of Seed Science ◽

10.1590/2317-1545v39n4173727 ◽

2017 ◽

Vol 39 (4) ◽

pp. 363-373 ◽

Cited By ~ 4

Author(s):

Jaciara de Souza Bispo ◽

Danielle Carolina Campos da Costa ◽

Samara Elizabeth Vieira Gomes ◽

Gilmara Moreira de Oliveira ◽

Janete Rodrigues Matias ◽

...

Keyword(s):

Electrical Conductivity ◽

Dry Matter ◽

Folk Medicine ◽

Germination Percentage ◽

Climate Variations ◽

Randomized Design ◽

Mother Plants ◽

Physiological Quality ◽

Completely Randomized Design

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.

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Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

Annual Research & Review in Biology ◽

10.9734/arrb/2019/v32i130075 ◽

2019 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

S. N. Mahadi ◽

F. Zawawi ◽

R. Nulit ◽

M. H. Ibrahim ◽

N. I. Ab. Ghani

Keyword(s):

Oryza Sativa ◽

Seed Germination ◽

Germination Percentage ◽

Leaf Length ◽

Seed Vigor ◽

Rice Seed ◽

Combination Treatments ◽

Randomized Design ◽

Completely Randomized Design ◽

Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

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PHYSIOLOGICAL QUALITY OF Brachiaria brizantha SEEDS TREATED WITH FUNGICIDE AND INSECTICIDE

Revista Caatinga ◽

10.1590/1983-21252018v31n313rc ◽

2018 ◽

Vol 31 (3) ◽

pp. 651-656

Author(s):

ERIC FABIANO SERAGUZI ◽

CARLOS HENRIQUE QUEIROZ REGO ◽

FERNANDA BRITO CARDOSO ◽

ANA CARINA DA SILVA CÂNDIDO ◽

CHARLINE ZARATIN ALVES

Keyword(s):

Root Development ◽

Dry Mass ◽

Brachiaria Brizantha ◽

Thiophanate Methyl ◽

Physiological Characterization ◽

Randomized Design ◽

Phytotoxic Effect ◽

Physiological Quality ◽

Completely Randomized Design

ABSTRACT This study aimed to evaluate the effects of chemical treatment of seeds with fungicide and insecticide on the seed physiological quality of Brachiaria brizantha cultivar MG5. Two experiments with four replicates were carried out in a completely randomized design. In the first experiment, the seeds were treated with the fungicide pyraclostrobin + fipronil + thiophanate-methyl and in the second, with the insecticide thiamethoxam, both at doses of 0, 150, 300, 450, and 600 mL of commercial product (CP)/100 kg of seeds. Physiological characterization was done on the basis of the first germination count, germination (%), emergence (%), emergence speed index, and length and dry mass of shoot and root. The treatment of B. brizantha seeds with the fungicide pyraclostrobin + fipronil + thiophanate-methyl benefits the physiological quality of seeds, improving germination and root development, with no phytotoxic effect up to the dose of 600 mL of CP/100 kg of seeds. The insecticide thiamethoxam has a biostimulating effect on B. brizantha cultivar MG5 up to the dose of 270 mL of CP/100 kg of seeds, but is phytotoxic in larger doses.

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Reduction of the Forage Seeds Physiological Quality by the Contact with Fertilizer in the Crop-Livestock Integration

Journal of Experimental Agriculture International ◽

10.9734/jeai/2019/v34i630190 ◽

2019 ◽

pp. 1-11

Author(s):

Anne Caroline Dallabrida Avelino ◽

Dayana Aparecida De Faria ◽

Carlos Eduardo Avelino Cabral ◽

Joadil Gonçalves De Abreu ◽

Elisangela Clarete Camili ◽

...

Keyword(s):

Electrical Conductivity ◽

Contact Time ◽

Accelerated Aging ◽

Germination Percentage ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Completely Randomized Design ◽

Seeds Germination

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.

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Vigor tests in seeds creole of Phaseolus lunatus L.

Bioscience Journal ◽

10.14393/bj-v35n5a2019-42309 ◽

2019 ◽

Vol 35 (5) ◽

Author(s):

Maria das Graças Rodrigues do Nascimento ◽

Maria Lúcia Maurício da Silva ◽

Edna Ursulino Alves ◽

Caroline Marques Rodrigues ◽

Maria Joelma da Silva

Keyword(s):

Electrical Conductivity ◽

Water Content ◽

Dry Mass ◽

Phaseolus Lunatus ◽

High Productivity ◽

Randomized Design ◽

Seed Analysis ◽

Seedling Length ◽

Physiological Quality

Seeds with a good physiological quality are essential to high productivity. However, for some seeds, Phaseolus lunatus L., the viability and vigor analyze show deficiency in quality. This work aimed to evaluate the physiological quality of Phaseolus lunatus L. seeds using several vigor tests. The study was realized at the Laboratory of Seed Analysis of the Agricultural Sciences Center of the Federal University of Paraíba, in Areia – PB, Brazil. We used seeds from five cultivars of P. lunatus (Branca, Orelha de Vó, Cearense, Rosinha, and Roxinha). To determine the physiological quality, we measured the water content, the viability and vigor test (germination, emergence, electrical conductivity, tetrazolium, first germination count and emergence, seedling length and dry mass). We used a completely randomized design for seeds evaluation, and the field test was performed in randomized blocks. The water content of P. lunatus did not differ among the cultivars, indicating a uniformity in the percentage of moisture. Also, the percentage and speed of emergency and the dry mass of seedlings did not differ among the varieties. The tetrazolium and electrical conductivity tests indicated that Rosinha cultivar had the worst viability concerning the other cultivars, showing a low percentage of living tissue and lower electrical conductivity. The seeds of Roxinha cultivar had the better physiological quality, and the Cearense cultivar had the worst quality evaluation. We recommend the tetrazolium test but not the electrical conductivity test to evaluate the vigor of P. lunatus.

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Physiological characteristics of endive, lettuce and chicory seeds as a function of spectral quality

Comunicata Scientiae ◽

10.14295/cs.v11i.3227 ◽

2020 ◽

Vol 11 ◽

pp. e3227

Author(s):

Matheus Milani Pretto ◽

Daniele Cristina Fontana ◽

Jullie Dos Santos ◽

Axel Bruno Mariotto ◽

Braulio Otomar Caron ◽

...

Keyword(s):

Dry Mass ◽

Germination Percentage ◽

Spectral Quality ◽

Second Stage ◽

Randomized Design ◽

Seedling Length ◽

Red Led ◽

Third Stage ◽

Significant Length

Seedling production is a critical step in the establishment of vegetables. This work aimed to evaluate the effect of different spectral qualities on the germination and vigor of endive, lettuce, and chicory seeds. The experiment was carried out in three stages. The first stage, two cultivars of lettuce (‘Crespa Repolhuda’ and ‘Vera’) and six spectral qualities (blue LED, red LED, blue + red LED, white LED, fluorescent and dark) were evaluated; the second stage, two cultivars of chicory (‘Lisa Escarola’ and ‘Palla Rosa’) and the same spectral qualities were evaluated. In the third stage, the spectral quality of the endive cultivar ‘Pão de Açúcar’ was evaluated. The experiments were performed in a completely randomized design, with four replications of 50 seeds each. The evaluated parameters were: germination percentage, first count, germination speed index, seedling length, and fresh and dry mass. Endive, lettuce and chicory seeds germinated both in the presence or not of light so that they can be classified as neutral photos. A spectral LED of red quality fostered the development of the most significant volume of fresh mass on the endive, lettuce, and chicory. All the spectral qualities stimulated root growth. The dark, on the other hand, promoted the most significant length of the aerial part, promoting seedlings etiolation.

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Physiological quality of parsley seeds in function of size

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n3supl1p1499 ◽

2021 ◽

Vol 42 (3Supl1) ◽

pp. 1499-1510

Author(s):

Renato Téo de Barros ◽

◽

Cibele Chalita Martins ◽

Tatiane Sanches Jeromini ◽

Givanildo Zildo da Silva ◽

...

Keyword(s):

Seed Size ◽

Dry Weight ◽

Germination Percentage ◽

Randomized Design ◽

Physiological Quality ◽

Completely Randomized Design ◽

Germination Vigor ◽

Selection Of

Parsley seeds are commercialized without classification by size during processing, unlike other crops. This classification procedure allows the standardization of seed size for high precision in sowing, disposal of unwanted material, and selection of high quality seeds. The aim of this study was to verify the effect of seed size on germination, vigor, and yield performance of two parsley cultivars. Seeds from eight lots of parsley of ‘Portuguesa’ and ‘Chacareira’ cultivar were separated into three size classes, represented by the seeds retained in the sieves of 0.84, 1.00 and 1.41mm mesh opening. After classification, seeds were evaluated in the laboratory for water content, germination percentage, first count, and thousand seed weight. In the field, the fresh and dry weight of aerial part and the final number of plants (stand) were evaluated after 78 days of sowing. The experimental design was the completely randomized design in an 8 x 3 factorial scheme (eight lots and three sieve opening) with four replicates, for both cultivars. Data obtained were subjected to analysis of variance and means were compared using Tukey test at 5% probability level. It was concluded that the parsley seeds separated by the 1.41 mm sieves showed higher vigor, and consequently, greater success in stand establishment at the field than those separated by other sieve size. The use of sieves in the classification of parsley seeds helps in obtaining lots with superior physiological quality.

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