scholarly journals Molecular identification of Ehrlichia sp. in a beef cattle from brazilian Cerrado - case report

2019 ◽  
Vol 71 (6) ◽  
pp. 1940-1943
Author(s):  
G. Oliveira ◽  
A.W. Silveira ◽  
S. Sorgatto ◽  
M.A. Araújo ◽  
T.R. Antunes ◽  
...  
Keyword(s):  
Natural Infection ◽  
Clinical Signs ◽  
Boophilus Microplus ◽  
Molecular Evidence ◽  
Evidence Based ◽  
Brazilian Cerrado ◽  
Babesia Bigemina ◽  
Pathogenic Potential ◽  
Mato Grosso ◽  
Mato Grosso Do Sul

ABSTRACT Ehrlichia infections in cattle are frequent in Africa but have also been reported in Brazil and North America. This paper reports natural infection by Ehrlichia sp. associated with Babesia bigemina and Anaplasma marginale in a calf in the municipality of Campo Grande, state of Mato Grosso do Sul, Brazil, presenting polioencephalomalacia. The molecular evidence, based on a fragment of the dsb gene, indicates a species of Ehrlichia genetically related to Ehrlichia canis and other species of the genus found in the tick Rhipicephalus (Boophilus) microplus and a calf from Brazil (99 to 100% identity). It was not possible to associate the clinical signs with Ehrlichia infection due to co-infections and histological evidence of another disease. However, the circulation of the bacteria in bovines in Brazilian Cerrado was confirmed and more attention should be given to clinical suspicion of tick-borne pathogens in cattle to clarify the pathogenic potential of Ehrlichia sp.

scholarly journals Evaluation of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) resistance to different acaricide formulations using samples from Brazilian properties

2016 ◽  
Vol 25 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Leandro de Oliveira Souza Higa ◽  
Marcos Valério Garcia ◽  
Jacqueline Cavalcante Barros ◽  
Wilson Werner Koller ◽  
Renato Andreotti
Keyword(s):  
Immersion Test ◽  
Minas Gerais ◽  
Boophilus Microplus ◽  
Economic Losses ◽  
Mato Grosso ◽  
Main Method ◽  
Espírito Santo ◽  
Distrito Federal ◽  
Espirito Santo ◽  
Mato Grosso Do Sul

Abstract The Rhipicephalus (Boophilus) microplus tick is responsible for considerable economic losses in Brazil, causing leather damage, weight loss and reduced milk production in cattle and results in the transmission of pathogens. Currently, the main method for controlling this tick is using acaricides, but their indiscriminate use is one of the major causes of resistance dissemination. In this study, the adult immersion test (AIT) was used to evaluate resistance in ticks from 28 properties located in five different states (Mato Grosso do Sul, Mato Grosso, Ceará, São Paulo, e Minas Gerais) and the Distrito Federal (DF) of Brazil. The resistance was found in 47.64% of the repetitions demonstrating an efficacy of less than 90% in various locations throughout the country. The larvae packet test was used to evaluate samples from ten properties in four states (Mato Grosso do Sul, Mato Grosso, Minas Gerais and Espírito Santo). Spray products belonging to the main classes of acaricides, including combination formulations, were used in both types of test. New cases of resistance were found on properties within the states of Ceará, Espírito Santo and Mato Grosso, where such resistance was not previously reported.

scholarly journals Sampling grid density and lime recommendation in an Oxisol

2014 ◽  
Vol 18 (11) ◽  
pp. 1142-1148 ◽  
Author(s):  
Eduardo L. Bottega ◽  
Daniel M. de Queiroz ◽  
Francisco A. C. Pinto ◽  
Antonio M. de Oliveira Neto ◽  
Cesar C. Vilar ◽  
...  
Keyword(s):  
Soil Analysis ◽  
Sample Point ◽  
Brazilian Cerrado ◽  
Mato Grosso ◽  
Sampling Grid ◽  
Sampling Points ◽  
Regular Sampling ◽  
Geostatistical Techniques ◽  
Mato Grosso Do Sul ◽  
Kriging Maps

The objective of this study was to evaluate the influence of different sampling grids density in the lime requirements in an Oxisol. The experiment was conducted at a rural property located in Sidrolândia city, Mato Grosso do Sul state, in the Brazilian ‘Cerrado’. In the soil attributes mapping, regular sampling grid was used consisting of 99 points, spread over an area of 90 ha. Other two grids (51 and 27 points) were derived by deleting lines or lines and points from the original one. Based on the results of soil analysis, the lime requirement at each sample point was calculated. Using geostatistical techniques the spatial variability of lime requirement was studied and grid configuration for each sample was tested. By kriging, maps were made. By reducing the number of sampling points, 11% of the experimental area showed an overestimation and 8% underestimation comparing with the lime requirement made using the highest sampling grid density.

scholarly journals Natural infection of phlebotomines (Diptera: Psychodidae) in a visceral-leishmaniasis focus in Mato Grosso do Sul, Brazil

2007 ◽  
Vol 49 (2) ◽  
pp. 119-122 ◽  
Author(s):  
João Cezar do Nascimento ◽  
Byanca Regina de Paiva ◽  
Rosely dos Santos Malafronte ◽  
Wedson Desidério Fernandes ◽  
Eunice Aparecida Bianchi Galati
Keyword(s):  
Visceral Leishmaniasis ◽  
Natural Infection ◽  
Leishmania Species ◽  
Common Species ◽  
The Other ◽  
Lutzomyia Longipalpis ◽  
Leishmania Chagasi ◽  
Mato Grosso ◽  
Negative Results ◽  
Mato Grosso Do Sul

The main purpose of this study was to investigate natural infection by Leishmania in phlebotomine females in a visceral-leishmaniasis focus in Antonio João county in Mato Grosso do Sul State, Brazil. Between June and October 2003, the digestive tracts of 81 females captured in Aldeia Campestre, Aldeia Marangatu and Povoado Campestre were dissected. The females were separated by species, location, area and date of capture into 13 groups and kept in ethanol 70%. To identify the Leishmania species using the PCR technique, amplifications of the ribosomal-DNA (rDNA) and mini-exon genes were analyzed. Of the 81 specimens, 77 (95%) were Lutzomyia longipalpis, making this the most common species; only one specimen of each of the species Brumptomyia avellari, Evandromyia cortelezzii, Evandromyia lenti and Nyssomyia whitmani was found. Trypanosomatids were identified in eight of the nine groups of Lutzomyia longipalpis (10.39%) one group from Aldeia Campestre, one from Aldeia Marangatu and six from Povoado Campestre; of the eight groups, one from Aldeia Marangatu and another, with promastigotes forms also confirmed by dissection (1.23%) from Povoado Campestre, were identified by PCR as Leishmania chagasi (2.6%). The other groups gave negative results. These findings indicate that there is a high risk of leishmaniasis transmission in this area.

scholarly journals Current status of ticks and tick-host relationship in domestic and wild animals from Pantanal wetlands in the state of Mato Grosso do Sul, Brazil

2017 ◽  
Vol 107 (suppl) ◽  
Author(s):  
Paulo Henrique Duarte Cançado ◽  
João Luiz Horácio Faccini ◽  
Guilherme de Miranda Mourão ◽  
Eliane Mattos Piranda ◽  
Valéria Castilho Onofrio ◽  
...  
Keyword(s):  
Scientific Literature ◽  
Domestic Animals ◽  
Boophilus Microplus ◽  
Current Status ◽  
Good Representation ◽  
Hard Tick ◽  
Study Region ◽  
Mato Grosso ◽  
Mato Grosso Do Sul ◽  
Amblyomma Tigrinum

ABSTRACT This is a commented list of tick’s species collected on various wild and domestic animals, including the reports on scientific literature for the studied region. Most of animals were small or medium mammals. Carnivores were the main taxa group examined. Although, the pampas deer ( Ozotocerus bezoarticus) and giant anteater ( Mymercophaga tridactyla) also has a good representation on study. Among domestic animals, dogs, horses and cattle were examined. Summing up, 18 tick species were listed for the region. Sixteen were hard ticks (Ixodidae) and two soft ticks (Argasidae). Amblyomma sculptum was the most common and abundant hard tick. Ornithodoros rostratus (Argasidae) was very abundant, being the more important Argasidae tick on the study region. The following species were colleted or reported on scientific literature: Argas miniatus Koch, 1844; Ornithodoros rostratus Aragão, 1911; Dermacentor nitens Newmann, 1897; Rhipicephalus ( Boophilus) microplus Canestrini, 1887; Amblyomma tigrinum Koch, 1844; A. dissimile Koch, 1844; A. ovale Koch, 1844; A. pauvum Aragão, 1908; A. sculptum Berlese, 1888; A. calcaratum Neumann, 1899; A. coelebs Neumann, 1899; A. dubittatum Newmann, 1899; A. scalpturatum Newmann, 1906; A. naponense Packard, 1869; A. nodosum Newmann, 1899; A. pseudoconcolor Aragão, 1908; A. rotundatum Koch, 1844; A. triste Koch, 1844.

scholarly journals A Species of the Genus Eubulus (Coleoptera: Curculionidae): A New and More Destructive Cassava Pest in the Brazilian Cerrado

2019 ◽  
Vol 112 (6) ◽  
pp. 3007-3011
Author(s):  
Charles M Oliveira ◽  
Eduardo A Vieira ◽  
Josefino F Fialho ◽  
Marina R Frizzas
Keyword(s):  
Management Strategies ◽  
Federal District ◽  
Minas Gerais ◽  
Brazilian Cerrado ◽  
Mato Grosso ◽  
Potential Losses ◽  
Mato Grosso Do Sul ◽  
New Pest ◽  
Future Management

Abstract In 2009, a new pest of cassava crops was observed in the Federal District (Brazil) and identified as Eubulus (Kirsch) sp. (Coleoptera: Curculionidae). The pest is currently distributed in the states of Goiás, Minas Gerais, Mato Grosso do Sul, and Paraná. The larvae consume roots, causing the tissue to rot and making it unfit for consumption. The damage can extend to 100% of the roots. Owing to the potential losses that this pest is capable of causing, studies are needed on its biology, ecology, and future management strategies aimed at minimizing the losses, allowing the continuity of cassava cultivation in Brazil. This study reports on the occurrence of the pest in Brazilian Cerrado, its distribution in Brazil, the symptoms of its infestation and estimates the damage caused.

scholarly journals Characterization of the pyrethroid resistance profile of Rhipicephalus (Boophilus)microplus populations from the states of Rio Grande do Sul and Mato Grosso do Sul, Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Márcia Cristina Mendes ◽  
Fernanda Calvo Duarte ◽  
João Ricardo Martins ◽  
Guilherme Marcondes Klafke ◽  
Leonardo Costa Fiorini ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
Rio Grande ◽  
Boophilus Microplus ◽  
Cattle Tick ◽  
Synthetic Pyrethroids ◽  
Rio Grande Do Sul ◽  
Mato Grosso ◽  
Cattle Ticks ◽  
Mato Grosso Do Sul

Cattle ticks Rhipicephalus(Boophilus) microplus are mainly controlled in Brazil by means of acaricide products, without any official policies in this regard. Acaricides continue to be sold indiscriminately, and this has contributed towards making the problem of resistance widespread, thus making diagnosis and monitoring of tick resistance essential. Here, bioassays (larval packet test) were performed on tick populations from the states of Rio Grande do Sul and Mato Grosso do Sul regarding their susceptibility to cypermethrin, deltamethrin and flumethrin. All the tick samples tested showed resistance to cypermethrin (10) (resistance factor (RF) ranging from 5.6 to 80.3) and deltamethrin (10) (RF ranging from 2.4 to 83.1). Six out of eight populations were resistant to flumethrin (RF ranging from 3.8 to 8.2). PCR molecular analyses did not show any T2134A mutations in the voltage-gated sodium channel gene, in any of the sampled populations. The results from this study highlight the critical status of resistance of the cattle tick to synthetic pyrethroids in the regions studied. Further studies are needed to identify the mechanisms responsible for the resistant phenotypes observed in the bioassays. This was the first detection of flumethrin resistance in Brazil.

scholarly journals Suscetibilidade de Rhipicephalus (Boophilus) microplus a carrapaticidas em Mato Grosso do Sul, Brasil

2011 ◽  
Vol 41 (8) ◽  
pp. 1447-1452 ◽  
Author(s):  
Alberto Gomes ◽  
Wilson Werner Koller ◽  
Antonio Thadeu Medeiros de Barros
Keyword(s):  
Bos Taurus ◽  
Boophilus Microplus ◽  
Mato Grosso ◽  
Mato Grosso Do Sul

O carrapato bovino, Rhipicephalus (Boophilus) microplus, destaca-se dentre os ectoparasitas de importância econômica à pecuária nacional, tornando-se necessária a adoção de medidas de controle, particularmente em rebanhos Bos taurus e seus cruzamentos. O controle do carrapato tem sido cada vez mais difícil devido à constatação de populações resistentes aos diversos produtos em uso. Neste estudo, teve-se por objetivo conhecer a suscetibilidade desse carrapato a acaricidas em Mato Grosso do Sul. De outubro de 2003 a outubro de 2006, testes carrapaticidogramas foram realizados em onze das principais regiões pecuárias do estado. Nos testes de suscetibilidade, foi utilizada a técnica de imersão de adultos, com posterior avaliação de parâmetros biológicos. Foram testados doze acaricidas comerciais, abrangendo sete princípios ativos (isoladamente ou em associação), pertencentes a três grupos químicos: amidinas (amitraz), piretróides sintéticos (cipermetrina) e organofosforados (clorfenvinfós, clorpirifós, diazinon, diclorvós e etion). Baixa suscetibilidade foi detectada em todas as propriedades, evidenciando uma reduzida eficácia de todos os grupos químicos testados. Apesar da grande variação de suscetibilidade demonstrada pelas distintas populações a cada acaricida testado, foi possível observar um gradiente de eficácia desses produtos. Independente da classe, a eficácia média dos produtos com um único princípio ativo (19,94%-64,27%) foi, de modo geral, menor que a das associações, tanto entre piretróide e organofosforados (46,38%-82,68%), como exclusivamente entre organofosforados (85,28%-97,68%). A associação contendo piretróide, organofosforados, sinergista e repelente (cipermetrina + clorpirifós + butóxido de piperonila + citronelal) apresentou 100% de eficácia, embora testada em menor número de populações. Embora comparações com cepas suscetíveis não tenham sido efetuadas, a baixa suscetibilidade demonstrada aos distintos produtos testados sugere que a resistência do carrapato a diferentes classes esteja amplamente disseminada no Estado, motivo pelo qual recomenda-se a realização rotineira de testes de suscetibilidade antes da seleção e aplicação de produtos acaricidas para controle do carrapato.

scholarly journals Sand fly captures with Disney traps in area of occurrence of Leishmania (Leishmania) amazonensis in the state of Mato Grosso do Sul, mid-western Brazil

2010 ◽  
Vol 43 (5) ◽  
pp. 491-495 ◽  
Author(s):  
Maria Elizabeth Cavalheiros Dorval ◽  
Tulia Peixoto Alves ◽  
Geucira Cristaldo ◽  
Hilda Carlos da Rocha ◽  
Murilo Andrade Alves ◽  
...  
Keyword(s):  
Natural Infection ◽  
Leishmania Species ◽  
Leishmania Amazonensis ◽  
Sand Fly ◽  
Lutzomyia Longipalpis ◽  
Mato Grosso ◽  
Predominant Species ◽  
American Cutaneous Leishmaniasis ◽  
Phlebotomine Sandflies ◽  
Mato Grosso Do Sul

INTRODUCTION: The work was conducted to study phlebotomine fauna (Diptera: Psychodidae) and aspects of American cutaneous leishmaniasis transmission in a forested area where Leishmania (Leishmania) amazonensis occurs, situated in the municipality of Bela Vista, State of Mato Grosso do Sul, Brazil. METHODS: The captures were conducted with modified Disney traps, using hamster (Mesocricetus auratus) as bait, from May 2004 to January 2006. RESULTS: Ten species of phlebotomine sandflies were captured: Brumptomyia avellari, Brumptomyia brumpti, Bichromomyia flaviscutellata, Evandromyia bourrouli, Evandromyia lenti, Lutzomyia longipalpis, Psathyromyia campograndensis, Psathyromyia punctigeniculata, Psathyromyia shannoni and Sciopemyia sordellii. The two predominant species were Ev bourrouli (57.3%) and Bi flaviscutellata (41.4%), present at all sampling sites. Two of the 36 hamsters used as bait presented natural infection with Leishmania. The parasite was identified as Leishmania (Leishmania) amazonensis. CONCLUSIONS: Analysis of the results revealed the efficiency of Disney traps for capturing Bichromomyia flaviscutellata and the simultaneous presence of both vector and the Leishmania species transmitted by the same can be considered a predictive factor of the occurrence of leishmaniasis outbreaks for the human population that occupies the location.

scholarly journals Desenvolvimento de uma prova de imunoadsorção enzimática para detecção de anticorpos contra Babesia bovis

2000 ◽  
Vol 20 (4) ◽  
pp. 167-170 ◽  
Author(s):  
Cláudio R. Madruga ◽  
Flábio R. Araújo ◽  
Ana Paula C. Marques ◽  
Cristiano M.E. Carvalho ◽  
Flávia Q. Cusinato ◽  
...  
Keyword(s):  
Babesia Bovis ◽  
Babesia Bigemina ◽  
Mato Grosso ◽  
Mato Grosso Do Sul

Uma prova de imunoadsorção enzimática (ELISA) para detecção de anticorpos contra Babesia bovis foi desenvolvida e avaliada em comparação à imunofluorescência indireta (IFI). A sensibilidade e especificidade do ELISA, determinadas pela análise de 100 soros positivos de bovinos infectados experimentalmente com B. bovis e 108 soros negativos colhidos de bovinos livres de infecção por este hemoparasito, foram de 98,0% e 98,1%, respectivamente. Os valores preditivos positivo e negativo foram, respectivamente, 98,0% e 98,1% e a precisão do teste foi de 98,1%. Não foram detectadas reações cruzadas com 80 soros de bezerros experimentalmente inoculados com Babesia bigemina. O ELISA foi comparado à IFI usando 110 soros de rebanhos de área de estabilidade endêmica e 168 soros de rebanhos de áreas de instabilidade endêmica. Em ambos os casos, houve concordância significativa (P=0,631 e 0,4725, respectivamente) entre os resultados demonstrados pelos dois testes. Em um estudo epidemiológico realizado com o ELISA na região do Pantanal de Mato Grosso do Sul, com 1.365 soros de bovinos, 83,9% foram positivos para anticorpos contra B. bovis, caracterizando a região estudada como endemicamente estável.

scholarly journals Trypanosoma vivax infection dynamics in a cattle herd maintained in a transition area between Pantanal lowlands and highlands of Mato Grosso do Sul, Brazil

2008 ◽  
Vol 28 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Charles F. Martins ◽  
Cláudio R. Madruga ◽  
Wilson W. Koller ◽  
Flábio R. Araújo ◽  
Cleber O. Soares ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Experimental Period ◽  
Vivax Infection ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Trypanosoma Vivax ◽  
Mato Grosso ◽  
Transition Area ◽  
Infection Dynamics ◽  
Mato Grosso Do Sul

Trypanosoma vivax outbreaks in beef cattle in the Pantanal region of Mato Grosso do Sul state, Brazil, causes relevant economical impact due to weight loss, abortion and mortality. Cattle moved from the Pantanal to adjacent areas of this ecosystem for breeding and fattening is a common feature. Therefore an epidemiological study on breeding cows in the transition area between Pantanal lowland and adjacent highlands of Mato Grosso do Sul was performed to determine the T. vivax infection dynamics and outbreak risk. Three experimental groups were formed: Group 1 consisted of cows parasitologically negative by the Woo test and in the enzyme-linked immunosorbent assay for T. vivax antibody detection (Tv-ELISA-Ab); Group 2 parasitologically negative and positive in the Tv-ELISA-Ab; and in Group 3 cows were parasitologically positive and with positive reactions in the Tv-ELISA-Ab. During 24 months, the cows' dislodgment between the above established groups was monitored by Woo test and Tv-ELISA-Ab exams. The tabanid population was also monitored and the highest number occurred during the rainy season. Although parasitemias were detected only in the first four samplings of the experimental period, the cows could be considered as trypanotolerant, because no clinical signs were observed. Despite the higher T. vivax incidence during the dry season, no disease symptoms were seen. Even though T. vivax epidemiological situation in the herd was characterized as endemic with seasonal variation, the probability of outbreaks was null within the conditions of the study.

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