scholarly journals Trypanosoma vivax infection dynamics in a cattle herd maintained in a transition area between Pantanal lowlands and highlands of Mato Grosso do Sul, Brazil

2008 ◽  
Vol 28 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Charles F. Martins ◽  
Cláudio R. Madruga ◽  
Wilson W. Koller ◽  
Flábio R. Araújo ◽  
Cleber O. Soares ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Experimental Period ◽  
Vivax Infection ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Trypanosoma Vivax ◽  
Mato Grosso ◽  
Transition Area ◽  
Infection Dynamics ◽  
Mato Grosso Do Sul

Trypanosoma vivax outbreaks in beef cattle in the Pantanal region of Mato Grosso do Sul state, Brazil, causes relevant economical impact due to weight loss, abortion and mortality. Cattle moved from the Pantanal to adjacent areas of this ecosystem for breeding and fattening is a common feature. Therefore an epidemiological study on breeding cows in the transition area between Pantanal lowland and adjacent highlands of Mato Grosso do Sul was performed to determine the T. vivax infection dynamics and outbreak risk. Three experimental groups were formed: Group 1 consisted of cows parasitologically negative by the Woo test and in the enzyme-linked immunosorbent assay for T. vivax antibody detection (Tv-ELISA-Ab); Group 2 parasitologically negative and positive in the Tv-ELISA-Ab; and in Group 3 cows were parasitologically positive and with positive reactions in the Tv-ELISA-Ab. During 24 months, the cows' dislodgment between the above established groups was monitored by Woo test and Tv-ELISA-Ab exams. The tabanid population was also monitored and the highest number occurred during the rainy season. Although parasitemias were detected only in the first four samplings of the experimental period, the cows could be considered as trypanotolerant, because no clinical signs were observed. Despite the higher T. vivax incidence during the dry season, no disease symptoms were seen. Even though T. vivax epidemiological situation in the herd was characterized as endemic with seasonal variation, the probability of outbreaks was null within the conditions of the study.

scholarly journals LABORATORY DIAGNOSIS AND CLINICAL SIGNS OF CANINE VISCERAL LEISHMANIASIS IN DOGS EXAMINED AT THE CENTER FOR ZOONOSIS CONTROL IN CAMPO GRANDE – MS, BRAZIL

2012 ◽  
Vol 17 (4) ◽  
Author(s):  
Rodrigo Casquero Cunha ◽  
Renato Andreotti ◽  
Elaine Silva ◽  
Elisângela Pereira ◽  
Tayra Sato ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Urban Areas ◽  
Clinical Symptoms ◽  
Fluorescent Antibody ◽  
Clinical Signs ◽  
Primary Source ◽  
Laboratory Diagnosis ◽  
Leishmania Species ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mato Grosso

Visceral leishmaniasis is a type of zoonosis caused by several Leishmania species endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical signs of leishmaniasis in dogs in Campo Grande, Mato Grosso do Sul, Brazil. Samples from affected animals were analyzed using indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays to determine the optimal diagnostic tool for use on animals that present clinical symptoms. A predominance of clinical symptoms affecting the integumentary system was observed, and splenomegaly and hepatomegaly were the most important pathological signs. Among the diagnostic tests, the greatest agreement was seen between ELISA and IFA, followed by ELISA and PCR, and finally IFA and PCR. PCR diagnostic results showed the greatest extent of correlation with clinical signs, followed by ELISA and then IFA. When choosing a diagnostic method, veterinarians should consider the clinical signs and health status of the patient.

scholarly journals Clinical evaluation and reproductive indices of dairy cows naturally infected with Trypanosoma vivax

2017 ◽  
Vol 38 (5) ◽  
pp. 3031
Author(s):  
Jael Soares Batista ◽  
Carlos Iberê Alves Freitas ◽  
Jardel Bezerra da Silva ◽  
Tania Vasconcelos Cavalcante ◽  
Kaliane Alessandra Rodrigues de Paiva ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Clinical Signs ◽  
Vivax Infection ◽  
Reproductive Efficiency ◽  
Trypanosoma Vivax ◽  
Group I ◽  
Before And After ◽  
The Individual ◽  
Clinical Changes ◽  
Reproductive Indices

The present study was aimed to assess the clinical changes and reproductive indices of dairy cows naturally infected with Trypanosoma vivax in a herd located in the Central Sertão of Ceará, Brazil. For the study, 40 animals were selected from a herd consisting of 210 dairy cows, and subdivided into two groups: group I, consisting of 20 cows naturally infected with T. vivax, and group II, consisting of 20 uninfected cows. Data on the parameters of reproductive efficiency were evaluated using a farm database (Propad Profissional GP®), in which the individual reproductive characteristics of the animals were stored. Data collected both before and after the outbreak were compared. The cows from group I showed a sudden drop in milk production, mucosal pallor, depression, anorexia, and a significant increase in rectal temperature and decreased hematocrit during the parasitemia outbreak. These clinical signs of the disease disappeared over time. However, T. vivax was detected by polymerase chain reaction (PCR) in animals with a negative parasitological test one year after the occurrence. Comparison of the reproductive index data for cows from groups I and II before and after the outbreak showed significant differences in first postpartum estrus, service period, and intervals between deliveries. In addition, repetition of estrus and abortion were significantly associated with T. vivax infection. Analysis of the reproductive indices of cows in groups I and II suggests that T. vivax infection decreased the reproductive efficiency of the study cows.

scholarly journals Prevalence of hepatitis C virus infection in quilombo remnant communities in Central Brazil

2008 ◽  
Vol 50 (6) ◽  
pp. 359-360 ◽  
Author(s):  
Nádia R.S. Reis ◽  
Ana R.C. Motta-Castro ◽  
Ágabo M.C. Silva ◽  
Sheila A. Teles ◽  
Clara F.T. Yoshida ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hcv Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hcv Rna ◽  
Rna Detection ◽  
Mato Grosso ◽  
Central Brazil ◽  
Low Prevalence ◽  
Mato Grosso Do Sul

In order to determine the prevalence of hepatitis C virus (HCV) infection in quilombo remnant communities in Central Brazil, 1,007 subjects were interviewed in all 12 communities existing in Mato Grosso do Sul State, Central Brazil. Blood samples were collected and sera were tested for anti-HCV by enzyme-linked immunosorbent assay. Positive samples were retested for confirmation using a line immunoassay and were also subjected to HCV RNA detection. The prevalence of HCV infection was 0.2%. This finding shows a low prevalence of HCV infection in quilombo remnant communities in Central Brazil.

scholarly journals Spontaneous poisoning by Brachiaria decumbens in goats

2016 ◽  
Vol 36 (5) ◽  
pp. 389-396 ◽  
Author(s):  
Fábio B. Rosa ◽  
Mara I.B. Rubin ◽  
Tessie B. Martins ◽  
Ricardo A.A. de Lemos ◽  
Danilo C. Gomes ◽  
...  
Keyword(s):  
Weight Loss ◽  
Bile Ducts ◽  
Clinical Signs ◽  
Mesenteric Lymph Nodes ◽  
Brachiaria Decumbens ◽  
Mato Grosso ◽  
Mesenteric Lymph ◽  
Orange Yellow ◽  
Foamy Macrophages ◽  
Mato Grosso Do Sul

Abstract: An outbreak of Brachiaria decumbens poisoning in goats in the state of Mato Grosso do Sul, Brazil, is described. Out of a herd of about 1,000 goats, seven goats died after showing photosensitivity (3 out 7 goats) and icterus (4/7). Other clinical signs included lethargy, dehydration and weight loss (7/7). At necropsy there was either hepatomegaly or liver atrophy; orange, yellow or gray discoloration - were observed in the liver of six goats. Two had accentuation of lobular pattern and punctate, linear or stellar fibrous depressions were randomly distributedover the capsular surface. Macroscopic changesattributedto liver failure consisting of hydropericardium (2/7) and ascites (2/7) were observed. Histologically, there was accumulation of refringent crystals in the bile ducts and infiltration of foamy macrophages in the liver of all goats. Foamy macrophages were also present in the hepatic and mesenteric lymph nodes of two goats. The diagnosis of B. decumbens poisoning was based on epidemiology, clinical signs, necropsy findings and histopathology.

scholarly journals Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Ana Cláudia Gabriela Alexandre de Santis ◽  
Heitor Miraglia Herrera ◽  
Keyla Cartens Marques de Sousa ◽  
Luiz Ricardo Gonçalves ◽  
Nathani Cristina Baccarim Denardi ◽  
...  
Keyword(s):  
Blood Cells ◽  
Molecular Detection ◽  
Clinical Signs ◽  
Molecular Techniques ◽  
Domestic Cats ◽  
Mato Grosso ◽  
Blast Analysis ◽  
Significant Difference ◽  
Pcr Assays ◽  
Mato Grosso Do Sul

Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for ‘Candidatus Mycoplasma haemominutum’, 17 (11.2%) for M. haemofelis and 15 (9.9%) for ‘Candidatus M. turicensis’, from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.

scholarly journals Reactivation of latent bovine herpesvirus type 5 in cattle with polioencephalomalacia induced by ammonium sulphate

2007 ◽  
Vol 27 (10) ◽  
pp. 435-441 ◽  
Author(s):  
Nadia David ◽  
Sílvia O. Hübner ◽  
Franklin Riet-Correa ◽  
Danisa Halfen ◽  
Ricardo A. Lemos
Keyword(s):  
Ammonium Sulphate ◽  
Clinical Signs ◽  
Uninfected Control ◽  
Mato Grosso ◽  
Herpesvirus Type ◽  
Nasal Swabs ◽  
The Central Nervous System ◽  
The Brain ◽  
Bovine Herpesvirus Type 5 ◽  
Mato Grosso Do Sul

In the state Mato Grosso do Sul, Brazil, outbreaks of meningoencephalitis by BoHV-5 and polioencephalomalacia (PEM) display similar epidemiological features, suggesting that meningoencephalitis may be associated with reactivation of a latent BoHV-5 infection, during the development of PEM. To test this hypothesis, four 7-8 months old steers negative for BoHV-5 antibodies were inoculated intranasally with BoHV-5 and received amprolium from day 35 to day 105 after inoculation. Because PEM was not produced during this period, ammonium sulphate was given from day 114 to day 180 after inoculation. Two uninfected control steers received amprolium and ammonium sulphate for the same periods. All inoculated cattle developed antibodies against BoHV-5 after inoculation and the virus was isolated from nasal swabs, indicating that they were infected. Two inoculated steers had clinical signs of PEM after 118 and 146 days after virus inoculation. One was euthanized after a clinical manifestation period of seven days and had severe lesions of PEM and meningoencephalitis. BoHV-5 was isolated from the central nervous system of this animal. The other animal recovered but continued to manifest chronic signs of PEM and was euthanatized. On histological examination, the cerebral cortex, caudate nucleus and thalamus had multifocal areas of malacia and mild meningoencephalitis of the cortex. BoHV-5 was not isolated from the brain. One uninfected control steer had signs of neurological disease on day 158 and had lesions of PEM without meningoencephalitis at necropsy. The simultaneous production of PEM and diffuse meningoencephalitis, with isolation of BoHV-5, in one steer treated with ammonium sulphate, 118 days after BoHV-5 inoculation, suggests that latent BoHV-5 was reactivated in this animal submitted to experimental induction of PEM.

scholarly journals Molecular identification of Ehrlichia sp. in a beef cattle from brazilian Cerrado - case report

2019 ◽  
Vol 71 (6) ◽  
pp. 1940-1943
Author(s):  
G. Oliveira ◽  
A.W. Silveira ◽  
S. Sorgatto ◽  
M.A. Araújo ◽  
T.R. Antunes ◽  
...  
Keyword(s):  
Natural Infection ◽  
Clinical Signs ◽  
Boophilus Microplus ◽  
Molecular Evidence ◽  
Evidence Based ◽  
Brazilian Cerrado ◽  
Babesia Bigemina ◽  
Pathogenic Potential ◽  
Mato Grosso ◽  
Mato Grosso Do Sul

ABSTRACT Ehrlichia infections in cattle are frequent in Africa but have also been reported in Brazil and North America. This paper reports natural infection by Ehrlichia sp. associated with Babesia bigemina and Anaplasma marginale in a calf in the municipality of Campo Grande, state of Mato Grosso do Sul, Brazil, presenting polioencephalomalacia. The molecular evidence, based on a fragment of the dsb gene, indicates a species of Ehrlichia genetically related to Ehrlichia canis and other species of the genus found in the tick Rhipicephalus (Boophilus) microplus and a calf from Brazil (99 to 100% identity). It was not possible to associate the clinical signs with Ehrlichia infection due to co-infections and histological evidence of another disease. However, the circulation of the bacteria in bovines in Brazilian Cerrado was confirmed and more attention should be given to clinical suspicion of tick-borne pathogens in cattle to clarify the pathogenic potential of Ehrlichia sp.

scholarly journals Detection of Humoral Antigen and Antibody by Enzyme-Linked Immunosorbent Assay in Horses with Experimentally Induced Ehulichia Equi Infection

1993 ◽  
Vol 5 (1) ◽  
pp. 37-39 ◽  
Author(s):  
Richard E. Corstvet ◽  
Stephen D. Gaunt ◽  
Phillip A. Karns ◽  
Jere W. McBride ◽  
Ricardo A. Battistini ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Rectal Temperature ◽  
Diagnostic Method ◽  
Serum Antibody ◽  
Clinical Signs ◽  
Antigen Detection ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ehrlichia Equi

An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.

A Synopsis of Dicliptera (Acanthaceae) in Brazil, with the Description of Two New Species

2020 ◽  
Vol 45 (2) ◽  
pp. 340-348
Author(s):  
James Lucas da Costa-Lima ◽  
Earl Celestino de Oliveira Chagas
Keyword(s):  
New Species ◽  
South America ◽  
The State ◽  
Northeastern Brazil ◽  
Mato Grosso ◽  
The North ◽  
New Synonyms ◽  
Two New Species ◽  
Mato Grosso Do Sul ◽  
A New Species

Abstract—A synopsis of Dicliptera (Acanthaceae) for Brazil is presented. Six species are recognized: Dicliptera ciliaris, D. sexangularis, and D. squarrosa, widely distributed in South America; D. purpurascens, which ranges from the North Region of Brazil (in the state of Acre) to eastern Bolivia; D. gracilirama, a new species from the Atlantic Forest of northeastern Brazil; and D. granchaquenha, a new species recorded in dry and semideciduous forests in Bolivia and western Brazil, in the state of Mato Grosso do Sul. Furthermore, we propose new synonyms and designate lectotypes for eleven names. An identification key to the six accepted Dicliptera species in Brazil is provided.

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