scholarly journals Comparison of subcutaneous inflammatory response to commercial and engineered zinc hydroxyapatite implants in rabbits

2019 ◽  
Vol 71 (6) ◽  
pp. 1873-1879
Author(s):  
L.C. Muñoz ◽  
S. Cardona.-Ramirez ◽  
R.F. Silva
Keyword(s):  
Bone Repair ◽  
Subcutaneous Tissue ◽  
Zinc Nitrate ◽  
Tissue Response ◽  
Polymorphonuclear Cells ◽  
Metal Compounds ◽  
Zn Concentration ◽  
Femur Diaphysis ◽  
Synthetic Hydroxyapatite ◽  
Histopathologic Analysis

ABSTRACT Hydroxyapatite (HA) is widely used as a biomaterial for bone repair and metallic prostheses coating. The main limitations of the current commercial synthetic hydroxyapatite compounds include high cost and decreased availability, especially for veterinary medicine purposes. Additionally, it is thought that HA biocompatibility and bioactivity could be enhanced by the addition of metal compounds. The objective of this work was to compare the subcutaneous tissue response of commercial and engineered hydroxyapatite obtained from the bovine femur diaphysis mixed with different concentrations of hexa-hydrated Zinc Nitrate in rabbits. Twenty-Five New Zealand female rabbits were used. Five treatments were done according to HA composition (commercial HA, no Zn-HA, 0.1M Zn, 0.2M Zn, and 0.3M Zn). Each treatment was evaluated at five time-points (8, 15, 30, 60 and 90 days post-implantation). Histopathologic analysis was performed to assess inflammation by polymorphonuclear cells infiltration, neovascularization, and fibrosis. Results obtained in this work suggest that general inflammation decreased after 60 days of implantation regardless of Zn concentration. Fibrosis score was increased in the commercial HP compared to control and Zn-hydrated HA. This paper shows that bovine hydroxyapatite is a biocompatible material regardless of nitrate Zinc concentration and has the same properties of commercial hydroxyapatite.

A Novelty of Synthetic Hydroxyapatite from Cockle Shell and Characterization

2015 ◽  
Vol 1087 ◽  
pp. 429-433 ◽  
Author(s):  
Rusnah Mustaffa ◽  
Mohd Reusmaazran Mohd Yusof ◽  
Yusof Abdullah
Keyword(s):  
Bone Graft ◽  
Bone Repair ◽  
Artificial Bone ◽  
Synthetic Bone ◽  
Potential Source ◽  
Mineral Constituent ◽  
Bone Graft Substitutes ◽  
Synthetic Hydroxyapatite ◽  
Anadara Granosa ◽  
Cockle Shell

In Malaysia recently, it was found that cockle shell (Anadara granosa) is a potential source of biomaterial for bone repair. It is the most abundant sea species cultured in Malaysia. A possible advantage of using cockle shell as a biomaterial is that they may act as an antilog of calcium carbonate. Malaysian Nuclear Agency took this challenge to develop synthetic bone graft from natural cockle shell. To date, the artificial bone graft substitutes developed from hydroxyapatite (Ca10(PO4)6(OH)2) a bio ceramic is similar to the mineral constituent of human bone. The structure and the composition of hydroxyapatite (HA) are similar to the mineral phase of bone and, its bioactivity and biocompatibility makes it a preferred bone graft.

scholarly journals Photosensitizers and Exposure Times To Light Showed Tissue Compatibility In Isogenic Mice

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Raquel Assed Bezerra da Silva ◽  
Daniela Silva Barroso de Oliveira ◽  
Katharina Morant Holanda de Oliveira ◽  
Carolina Maschietto Pucinelli ◽  
Paulo Nelson Filho ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Chloride Solution ◽  
Light Exposure ◽  
Subcutaneous Tissue ◽  
Phase 1 ◽  
Tissue Response ◽  
Phase 2 ◽  
Small Magnitude ◽  
Tissue Compatibility

Objective: The aim of this study was to evaluate the subcutaneous tissue response after different protocols to photodynamic therapy (PDT). In Phase 1, were tested the diode laser (used for 1min) associated to the photosensitizer phenothiazine chloride solution (PCS) in different concentrations. In Phase 2 – the diode laser and LED were tested associated to two different photosensitizers, PCS and Curcumin, in different exposure times of light application. Material and Methods: After 7, 21 and 63-days the animals were euthanized and the subcutaneous tissue processed to histological analysis. Qualitative and semi-quantitative descriptions of the inflammatory process and immunohistochemical technique were performed. The obtained data were analyzed by Kruskal-Wallis and Dunn’s post-test (alpha = 0.5). Results: On Phase 1, the tissue response was very similar among the groups. For the inflammatory infiltrate, PCS with concentration of 10mg/mL exhibited the most intense reaction (p > 0.05). On Phase 2, at 7-days period, the analyzed parameters presented small magnitude and after 21 and 63-days, all the parameters demonstrated tissue compatibility. Conclusion: Both photosensitizers presented proper tissue compatibility regardless the different concentrations used on Phase 1 and different durations of light exposure on Phase 2.   Keywords Photodynamic therapy; Phenothiazine chloride solution; Curcumin; Isogenic mice; Subcutaneous tissue.

scholarly journals Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

2013 ◽  
Vol 9 (3) ◽  
pp. 5464-5474 ◽  
Author(s):  
Matilde Bongio ◽  
Jeroen J.J. van den Beucken ◽  
M. Reza Nejadnik ◽  
Zeinab Tahmasebi Birgani ◽  
Pamela Habibovic ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Guinea Pigs ◽  
Subcutaneous Tissue ◽  
Medullary Cavity ◽  
Tissue Response ◽  
Calcium Phosphate Nanoparticle

scholarly journals Comparative study of tissue response to polyglecaprone 25, polyglactin 910 and polytetrafluorethylene suture materials in rats

2002 ◽  
Vol 13 (2) ◽  
pp. 86-91 ◽  
Author(s):  
Hugo Nary Filho ◽  
Mariza Akemi Matsumoto ◽  
Aline Carvalho Batista ◽  
Luís César Lopes ◽  
Fernanda Costa Grizzo de Sampaio Góes ◽  
...  
Keyword(s):  
Suture Material ◽  
Oral Surgery ◽  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Biological Behavior ◽  
Dorsal Region ◽  
Suture Materials ◽  
Polyglactin 910 ◽  
Hematoxylin And Eosin ◽  
Selection Of

The authors evaluated the biocompatibility of three suture materials, polyglecaprone 25, polyglactin 910 and polytetrafluorethylene, implanted into subcutaneous tissue in the dorsal region of 20 Wistar albinus rats. After periods of 2, 7, 14 and 21 days, the rats were sacrificed and the specimens were processed for routine histotechnical analysis and stained with hematoxylin and eosin. The rate of fibrosis, angioblastic and fibroblastic proliferation, and also the intensity of inflammation were observed with the optic microscope. The results showed that polyglecaprone 25 suture material induced a mild inflammatory reaction, followed by polyglactin 910 and polytetrafluorethylene, respectively. Such biological behavior must be considered during the selection of the suture material to be used in oral surgery.

Subcutaneous tissue response in rats to an aluminoborate cement

1983 ◽  
Vol 10 (1) ◽  
pp. 41-50 ◽  
Author(s):  
TREVOR L. P. WATTS ◽  
BARRY W. BERTENSHAW
Keyword(s):  
Subcutaneous Tissue ◽  
Tissue Response

scholarly journals Enzyme histochemical study of multinucleated giant cells responding to synthetic hydroxyapatite in rat jaw bone and subcutaneous tissue.

1991 ◽  
Vol 33 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Takumi Akagi ◽  
Nobuyoshi Takeshita ◽  
Tetsundo Nojima ◽  
Toyokazu Ozeki ◽  
Osamu Fujii ◽  
...  
Keyword(s):  
Histochemical Study ◽  
Subcutaneous Tissue ◽  
Giant Cells ◽  
Multinucleated Giant Cells ◽  
Jaw Bone ◽  
Synthetic Hydroxyapatite

scholarly journals The Dynamics of Subcutaneous Tissue Response to Microorganisms Associated with the Extract of Araçá (Psidium cattleianum): An Edemogenic and Microscopic Analysis

2017 ◽  
Vol 20 (2) ◽  
pp. 93 ◽  
Author(s):  
Alessandra Cury Machado ◽  
Denise Belucio Ruviere ◽  
Renata Zoccal Novais ◽  
Carlos Roberto Emerenciano Bueno ◽  
Elerson Gaetti Jardim Jr ◽  
...  
Keyword(s):  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Male Rats ◽  
Saline Control ◽  
Control Group ◽  
Dorsal Region ◽  
Hydroalcoholic Extract ◽  
Psidium Cattleianum ◽  
Significant Difference

<p><strong>Objective:</strong> To evaluate <em>in vivo </em>tissue reaction to the extract of araçá (<em>Psidium cattleianum</em>) associated with inactivated microorganisms. <strong>Material and Methods:</strong> A 0.1 mL suspension was used containing Porphyromonas gingivalis, Prevotella intermedia, <em>Fusobacterium nucleatum, Enterococcus faecalis, Peptostreptococcus micros</em>, and <em>Porphyromonas endodontalis,</em> which were inactivated by heat and mixed into a 1.0 mL saline (control group), an aqueous solution, or a hydroalcoholic extract of araçá. Eighteen male rats (<em>Rattus norvegiccus</em>) under general anesthesia received 0.2 mL of 1% intravenous Evans blue. Thirty minutes later, 0.1 mL of one of the associations was injected into the animals’ dorsal region. The animals were euthanized after 3 and 6 hours, and the materials obtained were placed in formamide for 72 hours then analyzed in a spectrophotometer (λ=630 hm). For the morphological analysis, 30 rats received polyethylene tubes implants with the extracts or the saline with the associations in the dorsal region and euthanized after 7 and 30 days to be analyzed according to an inflammation cell score. <strong>Results:</strong> No significant difference (p&gt;0.05) was observed in the edema among groups. The optical microscopy results showed a repair in the 30-day-period, which was higher when compared to the 7-day-period (p&lt;0.0001). Nevertheless, in the 7-day-period, the hydroalcoholic extract presented a significant response compared to the aqueous extract (p=0.05) and a trend for better results than the control group. <strong>Conclusion: </strong>The aqueous and hydroalcoholic araçá extracts associated with inactivated microorganisms showed similar responses to control, indicating no interference on the toxic effects of the bacterial components in tissue repair.</p><p><strong>Keywords</strong></p><p>Anaerobic bacteria; Edema; Inflammation; Plant extracts; <em>Psidium.</em></p>

scholarly journals Analysis of tissue response around injectable hydrogels in the subcutaneous tissue of Wistar rats

2016 ◽  
Vol 4 ◽  
Author(s):  
Oliveira Newton ◽  
Duek Eliana ◽  
Aoki Khatharine ◽  
Gurfinkel Yuri ◽  
Mistura Daniel
Keyword(s):  
Wistar Rats ◽  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Injectable Hydrogels

scholarly journals In Vivo Investigation of Soft Tissue Response of Novel Silver/Poly(Vinyl Alcohol)/ Graphene and Silver/Poly(Vinyl Alcohol)/Chitosan/Graphene Hydrogels Aimed for Medical Applications – The First Experience

2018 ◽  
Vol 68 (3) ◽  
pp. 321-339 ◽  
Author(s):  
Tijana Lužajić Božinovski ◽  
Danica Marković ◽  
Vera Todorović ◽  
Bogomir Prokić Bolka ◽  
Ivan Milošević ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Vinyl Alcohol ◽  
Subcutaneous Tissue ◽  
Tissue Response ◽  
Surrounding Tissue ◽  
Poly Vinyl Alcohol ◽  
Medical Applications ◽  
Capsule Thickness ◽  
Soft Tissue Response

Abstract In this paper, we have shown for the fi rst time the soft tissue response of novel silver/ poly(vinyl alcohol)/graphene (Ag/PVA/Gr) and silver/poly(vinyl alcohol)/chitosan/ graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels aimed for medical applications. These novel hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices as described in our previously published works. Both Ag/PVA/Gr and Ag/PVA/CHI/Gr, as well as controls Ag/PVA, Ag/PVA/CHI and commercial Suprasorb©hydrogel discs, were implanted in the subcutaneous tissue of rats. Implants with the surrounding tissue were dissected after post-implantation on days 7, 15, 30 and 60, and then processed for histological examination. The tissue irritation index (TIrI) score, according to ISO 10993-6, 2007, as well as the number of leukocytes in the peri-implant zone and connective tissue capsule thickness were examined. The results show that each TIrI score, the leukocyte number around the implanted materials and capsule thickness gradually decreased during the observation period. At the endpoint of follow-up, the Ag/PVA/CHI/Gr implant was surrounded with a thinner capsule, while both the TIrI score and the number of leukocytes of the peri-implant zone were greater compared to the Ag/PVA/Gr implant. Despite the observed differences, we can conclude that our in vivo experiment suggested that both novel hydrogels were biocompatible and suitable for medical use.

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