scholarly journals Morphological characterization of fruit, seed and seedling, and seed germination test of Campomanesia guazumifolia

2018 ◽  
Vol 40 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Michele Trombin Souza ◽  
Mireli Trombin Souza ◽  
Maristela Panobianco

Abstract: Campomanesia guazumifolia (Cambess.) O. Berg. is a native fruit species, with ecological importance and potential to be exploited by food and medical industries. There is a lack of studies enabling its on-site conservation and propagation for commercial purposes. The objective of this work was to morphologically characterize the fruit, seed and seedling development stage of this species, as well as to determine an efficient protocol for the germination test, aiming at the commercial production of seedlings. Different temperatures (20, 25, 30 and 20-30 °C), substrates (blotting paper, sand and vermiculite) and light regimes (presence and absence) were tested in order to evaluate the germination power of seeds. Fruits are bacoid, rounded, with a fleshy pericarp and hairy surface, and with an intense yellow coloration. Seeds have a reniform shape and a lateral recess, with a membranous, brown and verrucose-glanulose tegument. It has a crass embryo, with a well developed hypocotyl-radicle axis and vestigial cotyledons. Seedlings are epigeal and phanerocotylar. It is recommended to conduct the germination test for the species with temperatures of 25 or 30 ºC, on paper substrates, with light presence or absence, and with the first test count performed 23 days and the final count 50 days after sowing.

2016 ◽  
Vol 76 (2) ◽  
pp. 367-373 ◽  
Author(s):  
A. B. Lone ◽  
R. C. Colombo ◽  
B. L. G. Andrade ◽  
L. S. A. Takahashi ◽  
R. T. Faria

Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.


Cerâmica ◽  
2007 ◽  
Vol 53 (328) ◽  
pp. 422-447
Author(s):  
F. C. D. Lemos ◽  
D. M. A. Melo ◽  
P. S. de Lima ◽  
C. A. Paskocimas ◽  
E. Longo ◽  
...  

Rare earth modified lead titanate powders Pb1-xRExTiO3 (REPT), x = 0.01, 0.05, 0.07 and RE = Yb, Y, were prepared by the Pechini method. The materials were calcined under flowing oxygen at different temperatures from 300 to 700 ºC. Nanostructured REPT were investigated using X-ray diffraction, scanning electron microscopy and surface area analysis (BET). The results suggest that the modifier cation incorporated into the system has notable influence in the microstructure and a notable decrease in the crystallite sizes.


2019 ◽  
Vol 41 (3) ◽  
pp. 344-351
Author(s):  
Marizangela Rizzatti Ávila ◽  
Juliana Barbosa ◽  
Nelson da Silva Fonseca Júnior ◽  
Getúlio Takashi Nagashima ◽  
Carolina Maria Gaspar de Oliveira ◽  
...  

Abstract: Adoption of a standard procedure to perform the germination test allows comparison of results between laboratories. Thus, the aim of this study was to evaluate the germination process of rangpur lime (Citrus limonia Osbeck) seeds conducted under different temperatures. The germination test was carried out in five seed lots under three temperatures (25, 30 and 35 °C). The dynamics of the germination process was studied through the following variables: germination (%), time to first germination (days), time to last germination (days), time interval between first and last germination (days), time required to reach 50% of germination (days), mean speed of germination (day-1), synchrony of the germination process (dimensionless), and uncertainty of the germination process (bits). The experimental design was completely randomized, and analysis of variance was performed in a 5 (lots) x 3 (temperatures) factorial arrangement, with four replicates of each treatment. Means were compared using Tukey’s test (p < 0.05). The germination process in rangpur lime seeds is slow, homogenous, and asynchronous; and the temperature of 30 °C is the most favorable for conducting the germination test, with the first count at 21 days and the last at 30 days.


2009 ◽  
Vol 1178 ◽  
Author(s):  
Olivia Maria Berengue ◽  
Cleocir J. Dalmaschio ◽  
Tiago G. Conti ◽  
Adenilson J. Chiquito ◽  
Edson R. Leite

AbstractSn3O4 nanobelts were grown by a carbothermal evaporation process of SnO2 powders in association with the well known vapour-solid mechanism (VS). The nanobelts crystal structure was investigated by x-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM), raman spectroscopy and field emission gun scanning electron microscopy (FEG-SEM). The structural and morphological characterization has confirmed the growth of single crystal nanobelts. The electrical characterization (current-voltage, temperature-dependent resistance curves) of individual Sn3O4 nanobelts was performed at different temperatures and light excitation. The experiments revealed a semiconductor – like character as evidenced by the resistance decreasing at high temperatures. The transport mechanism was identified as the variable range hopping.


Author(s):  
Turgut Yeşiloğlu ◽  
Yıldız Aka Kacar ◽  
Bilge Yılmaz ◽  
Meral İncesu ◽  
Berken Çimen

In this study, carried out in University of Cukurova, Faculty of Agriculture, Department of Horticulture, morphological and molecular characterization of 20 persimmon accessions were performed. To determine morphological characterization, plant development stage, growth, and brunch structure, characteristics of leaf, fruit and seed were investigated. Data obtained by morphological characterization were subjected to similarity coefficient, principal components and cluster analyses to demonstrate the overall phenotypic relationships among these genotypes. The first 3 components contained 55.11% of the total multivariate variation. According to a cluster analysis, the similarity index of the population consists of 20 persimmon genotypes ranged between 6.01% and 67.47%. According to molecular characterization by full name (RAPD) markers, high level of genetic polymorphism was determined in persimmons. Although the high polymorphism, close genetic relationships have been determined among some genotypes. According to the RAPD markers, 82% similarity level was identified between ‘Hana Fuyu’ (Fr) and ‘Hachiya’ (Fr). Also ‘Fuji’ variety has shown a similarity of 80% between these genotypes. In addition, ‘Kaki Tipo’ (Fr), 07 TH 05, TH 01 07 TH 06 and 33 genotypes were clustered in the same group, and similarity level has been determined to be above 65%.


2013 ◽  
Vol 35 (4) ◽  
pp. 524-531 ◽  
Author(s):  
Alex Caetano Pimenta ◽  
Suelen Santos Rego ◽  
Katia Christina Zuffellato-Ribas ◽  
Antônio Carlos Nogueira ◽  
Henrique Soares Koehler

Araticum (Annona crassiflora Mart. - Annonaceae) is a species with great ecological importance and potential for fruit production. Its fruits, seeds and seedlings were morphologically described in this article as being the main objective of this study. To describe and illustrate the examples, 100 seeds and 42 fruits were analyzed; the latter being collected in Santo Antônio do Leverger - MT - Brazil, in 2011. To describe the morphology of the seedling, 100 seeds were germinated in two different environments. The araticum plant has a compound, multiple strobiliform, globose-subglobose and fleshy fruit, with average measures of 12.9 cm length, 13.5 cm width, 12.7 cm thickness, weight of 1,187.0 g and 99.2 seeds per fruit. The seeds are obovoid, hairless, smooth with a bony aspect and a pale brown color; they have an average of 17. 7 mm length, 10.8 mm width and 8.3 thickness. Epigeous and phanerocotylar germination begins about 148 days after sowing. All morphological data of araticum fruits, seeds and seedlings can be used to recognize the botanical family and, when associated to other features, to recognize the species in the field.


2019 ◽  
Vol 40 (5) ◽  
pp. 1737
Author(s):  
Verônica Pellizzaro ◽  
Mônica Satie Omura ◽  
Felipe Favoretto Furlan ◽  
Denis Santiago da Costa ◽  
Raissa Marrafon Ponce ◽  
...  

Physalis peruviana L. is a plant that produces small sweet berries of great nutritional and medicinal importance. This plant is propagated mainly through seeds, which requires information on germination response to temperature and light changes. Thus, the present study aimed to evaluate the use of different temperatures and light wavelengths on the physiological potential of P. peruviana seed batches. The experiment was conducted with seeds from plants cultivated in 2015/15 and 2016/16. The treatments applied were the following: two germination temperatures (constant temperature of 25 °C and alternated between 20/30 °C), two seed batches (2015/15 and 2016/16), and four forms of light exposure during germination (blue, red, white, and dark). The data were analyzed by principal component analysis using the correlation matrix obtained through the standardized mean equal to zero and variance 1 of the parameters evaluated for each treatment. The eigenvalues and eigenvectors of the matrix were obtained using the SAS University Edition® statistical software. The analyzed variables were: Germination test performed on the 7th and 28th day considering root protrusion, normal seedlings, shoot length, root length, and total dry matter mass. The data showed that the seed germination test of Physalis peruviana L. should be performed under a constant temperature of 25 °C, in the presence of light (white, blue, or red). When the option is the application of the test in the dark, it is necessary to use a thermoperiod of 20–30 °C to provide the expression of maximum physiological potential.


10.5219/1096 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 634-643
Author(s):  
Matej Pospiech ◽  
Zdeňka Javůrková ◽  
Bohuslava Tremlová ◽  
Hana Běhalová

One of the options to determine botanical origin of trees or honey is the analysis of pollen grains. The characteristics of pollen grains in Czech flora has not been sufficiently described yet. Within this work, fruit trees pollen of Czech origin was characterized on the basis of morphological and spectral description of pollen grains produced by fruit species of M. domestica, P. armenica, P. persica, P. domestica, P. avium and P. cerasus. The morphological characterization results of the studied fruit species are consistent with results by other authors, but certain differences between the pollen grains of some fruit trees were confirmed. Most morphological differences were confirmed among the Malus and Prunus genera. Results of morphological and spectral analyzes further confirmed the differences between some types of fruit trees, but homogeneity remained for individual species even in mixed samples. Morphological and spectral analysis can therefore be used for botanical identification of pollen. If this knowledge is applied to pollen analysis in honey, these methods can also be used to verify the botanical origin of honey.


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
APPR Amarasinghe ◽  
RP Karunagoda ◽  
DSA Wijesundara

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