Dynamics of the rangpur lime seed germination test conducted under different temperatures

Abstract: Adoption of a standard procedure to perform the germination test allows comparison of results between laboratories. Thus, the aim of this study was to evaluate the germination process of rangpur lime (Citrus limonia Osbeck) seeds conducted under different temperatures. The germination test was carried out in five seed lots under three temperatures (25, 30 and 35 °C). The dynamics of the germination process was studied through the following variables: germination (%), time to first germination (days), time to last germination (days), time interval between first and last germination (days), time required to reach 50% of germination (days), mean speed of germination (day-1), synchrony of the germination process (dimensionless), and uncertainty of the germination process (bits). The experimental design was completely randomized, and analysis of variance was performed in a 5 (lots) x 3 (temperatures) factorial arrangement, with four replicates of each treatment. Means were compared using Tukey’s test (p < 0.05). The germination process in rangpur lime seeds is slow, homogenous, and asynchronous; and the temperature of 30 °C is the most favorable for conducting the germination test, with the first count at 21 days and the last at 30 days.

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Weibull distribution and dynamics of germination of Rangpur lime seeds at different temperatures

Journal of Seed Science ◽

10.1590/2317-1545v41n3214831 ◽

2019 ◽

Vol 41 (3) ◽

pp. 328-335

Author(s):

Marizangela Rizzatti Ávila ◽

Juliana Barbosa ◽

Nelson da Silva Fonseca Júnior ◽

Getúlio Takashi Nagashima ◽

Carolina Maria Gaspar de Oliveira

Keyword(s):

Weibull Distribution ◽

Cumulative Distribution Function ◽

Cumulative Distribution ◽

Germination Test ◽

Test Installation ◽

Rangpur Lime ◽

Randomized Design ◽

Germination Process ◽

Different Temperatures ◽

Completely Randomized Design

Abstract: Rangpur lime is the most commonly used rootstock in Brazil, and its propagation occurs through seeds. Currently, no methodology is described in the prescriptions of the Rules for Seed Testing to perform the germination test of Rangpur lime. Thus, this research aimed to study the dynamics of germination in Rangpur lime seeds according to the Weibull distribution under the effect of different temperatures and lots. The experiment was conducted in a completely randomized design, with treatments arranged in a 3 × 5 factorial scheme (temperatures of 25, 30 and 35 °C for the germination test and five seed lots, respectively) and four replications. The percentage of germination, the number of days required obtaining 50% of germination, and the parameters of the Weibull equation were determined. The optimum temperature for germination of Rangpur lime seeds was 30 °C, with the first and last counts at 21 and 30 days after test installation, respectively. The germination process can be described by the three-parameter Weibull cumulative distribution function.

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Phytotoxicity assessment of conventional and biodegradable plastic bags using seed germination test

Ecological Indicators ◽

10.1016/j.ecolind.2019.03.005 ◽

2019 ◽

Vol 102 ◽

pp. 569-580 ◽

Cited By ~ 15

Author(s):

Elena Balestri ◽

Virginia Menicagli ◽

Viviana Ligorini ◽

Sara Fulignati ◽

Anna Maria Raspolli Galletti ◽

...

Keyword(s):

Seed Germination ◽

Biodegradable Plastic ◽

Germination Test ◽

Plastic Bags ◽

Seed Germination Test

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Seed germination test for heavy metal phytotoxicity assessment

Canadian Journal of Plant Science ◽

10.4141/cjps-2014-018 ◽

2014 ◽

Vol 94 (8) ◽

pp. 1519-1521 ◽

Cited By ~ 6

Author(s):

Jichul Bae ◽

Gaston Mercier ◽

Alan K. Watson ◽

Diane L. Benoit

Keyword(s):

Heavy Metal ◽

Seed Germination ◽

Metal Toxicity ◽

Petri Dish ◽

Germination Test ◽

Test Species ◽

Centrifuge Tube ◽

Metal Treatment ◽

Agar Media ◽

Seed Germination Test

Bae, J., Mercier, G., Watson, A. K. and Benoit, D. L. 2014. Seed germination test for heavy metal phytotoxicity assessment. Can. J. Plant Sci. 94: 1519–1521. We introduced a germination assay for metal toxicity using micro-centrifuge tube and agar media as germination substrate. The additives associated with metal treatment preparation had no effect on germination of four test species. This method can circumvent some of the limitations derived from the traditional petri-dish germination assays.

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CONTROL OF Bipolaris oryzae (Breda de Haan) USING Curcuma longa (Linnaeus) EXTRACT AND EFFECT OF THIS EXTRACT ON RICE SEED PHYSIOLOGY

Revista Caatinga ◽

10.1590/1983-21252018v31n112rc ◽

2018 ◽

Vol 31 (1) ◽

pp. 99-105

Author(s):

KEILOR DA ROSA DORNELES ◽

PAULO CESAR PAZDIORA ◽

FÁBIO JÚNIOR ARAÚJO SILVA ◽

RENATA MOCCELLIN ◽

CÂNDIDA RENATA JACOBSEN FARIAS

Keyword(s):

Seed Germination ◽

Mycelial Growth ◽

Curcuma Longa ◽

Control Treatment ◽

Rice Seed ◽

Germination Test ◽

Bipolaris Oryzae ◽

Seed Physiology ◽

Seed Germination Test

ABSTRACT This study was conducted to evaluate the use of Curcuma longa (Linnaeus) extract in the in vitro control of Bipolaris oryzae (Breda de Haan) and to characterize the effect of this extract on rice seed germination. A completely randomized arranged in a factorial experimental design was used: three isolates of B. oryzae from rice seed from different rice-growing regions of Rio Grande do Sul (Fronteira Oeste, Campanha, and Sul) were tested with three concentrations (20, 40, and 80 mg/mL) of C. longa plus a control treatment (0 mg/mL). Each reaction was repeated in quadruplicate. The effect of the extract upon the disease development was evaluated based on mycelial growth (PMG) and spore production; rice seed germination was evaluated using a germination test (Germitest®). The PMG results demonstrate that the treatments were effective in reducing PMG, with a stronger response observed as the concentration of the extract increased. An average inhibition of 84% of sporulation was observed for the tested strains compared with the control treatment. There were, however, no significant differences in terms of seed germination test with the different C. longa concentrations. Therefore, treatment of rice seeds with C. longa extract does not affect seed germination but positively inhibits mycelial growth and sporulation, affecting the in vitro sporulation of the different isolates of B. oryzae.

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The Effect of Seed Sources Variation and Presowing Treatments on the Seed Germination ofAcacia catechuandElaeocarpus floribundusSpecies in Bangladesh

International Journal of Forestry Research ◽

10.1155/2014/984194 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Niamjit Das

Keyword(s):

Seed Germination ◽

Hot Water ◽

Cow Dung ◽

Germination Test ◽

Rural Bangladesh ◽

Seed Sources ◽

Germination Process ◽

Germination Success ◽

Acacia Catechu ◽

Source Variation

The seed germination of seed sources and presowing treatments ofAcacia catechuandElaeocarpus floribundusseeds were conducted in the nursery of Bangladesh Agricultural University. The seeds were collected from matured and healthy trees from four different locations in Bangladesh and treated with six presowing methods. The germination test was conducted in polybags with a mixture of topsoil and cow dung in a ratio of 3 : 1. The results of ANOVA showed no significant differences among seed sources but statistically significant differences among the presowing treatments for both species. Thus the presowing methods affected the germination process of seeds, and then the highest germination success was found to be 91.26% in hot water (80°C for 10 min), treatment inAcacia catechuand the highest germination success (89.81%) ofElaeocarpus floribunduswas found in H2SO4treatment followed by 86.35% and 78.42% in treatments with hot water (100°C for 12 min) and scarification. The study also revealed that the interactions between seed source variation and presowing methods effect significantly differed in seed germination percentages. Therefore, it is concluded that hot water treatment can be suggested on seed germination of both species for developing nurseries and rural Bangladesh.

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Composting of food waste in passive aerated bioreactor with turning mode

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/1195/1/012001 ◽

2021 ◽

Vol 1195 (1) ◽

pp. 012001

Author(s):

A Y Zahrim ◽

M Darwis ◽

D Samantha ◽

A Z Siti Hasanah ◽

S A Nur Aqeela ◽

...

Keyword(s):

Seed Germination ◽

Organic Carbon ◽

Plant Growth ◽

Moisture Content ◽

Total Organic Carbon ◽

Food Waste ◽

Physicochemical Characteristics ◽

Germination Test ◽

Index Value ◽

Seed Germination Test

Abstract Almost 45% of municipal solid waste in Malaysia consist of food waste. Composting is one of the sustainable ways to manage food waste compared to incineration and landfilling. This paper investigates the physicochemical and phytotoxicity characteristics during food waste composting in passive aerated bioreactor assisted with compost turning. The initial compost mixture consists of 124 kg of food waste mixed with 62 kg of dry leaves. The composting process was conducted for 40 days, and physicochemical characteristics i.e., temperature, moisture content, total organic carbon, pH and conductivity were monitored. Seed germination test was conducted with cabbage seeds (Brassica oleracea). The highest temperature and final moisture content obtained were 42 °C and 78%, respectively. The seed germination index value was 127%, indicating that the compost is suitable for plant growth.

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GERMINAÇÃO DE SEMENTES DE PAU-DE-ESPETO (Casearia gossypiosperma) EM DIFERENTES TEMPERATURAS

FLORESTA ◽

10.5380/rf.v45i1.27599 ◽

2014 ◽

Vol 45 (1) ◽

pp. 97

Author(s):

Ademir Kleber Morbeck Oliveira ◽

Juliana Santos Souza ◽

Júnior Manoel Braga Carvalho ◽

Simone Alves Souza

Keyword(s):

Seed Germination ◽

Economic Potential ◽

Mato Grosso ◽

Lack Of Information ◽

Germination Process ◽

Germination Speed ◽

Different Temperatures ◽

Ecological Importance ◽

Mato Grosso Do Sul ◽

Forest Seeds

Casearia gossypiosperma Briq., uma espécie arbórea pertencente à família Salicaceae, é utilizada na construção civil, arborização e paisagismo. Em virtude de sua importância econômico-ecológica e da escassez de informações sobre seu processo germinativo, o presente trabalho foi realizado com o objetivo de determinar as temperaturas mais adequadas para a germinação de sementes de pau-de-espeto. As sementes utilizadas no experimento foram coletadas no Pantanal de Miranda, Mato Grosso do Sul, Brasil. Em seguida, elas foram submetidas a seis tratamentos, com temperaturas constantes de 20, 25, 30 e 35 °C e alternadas de 20-30 e 25-35 °C, em câmaras de germinação. Os resultados obtidos evidenciaram maior porcentagem de germinação das sementes nas temperaturas de 25 e30 °C, atingindo 83 e 84%, maior velocidade de germinação (10,0 em 25 °C e 10,7 em 30 °C) e comprimento de plântula (10,3 mm em 25 °C e10,8 mm em 30 °C). Portanto, a faixa de temperatura ótima para a germinação de sementes de pau-de-espeto está entre 25 e 30 ºC.Palavras-chave: Pantanal; sementes florestais; formação de plântulas; Salicaceae. AbstractGermination of Casearia gossypiosperma seeds at different temperatures. The species Casearia gossypiosperma is a tree, Salicaceae family, and its wood is suitable for civil construction, afforest, and landscaping. Due to its economic potential, ecological importance, and lack of information on its germination process, the aim of this research was to evaluate seed germination at different temperatures. The seeds used were collected from Pantanal de Miranda, Mato Grosso do Sul – Brazil and were submitted to six different temperatures, constant of 20, 25, 30 and35 °C and alternating, 20-30 and 25-35 °C, in germination chambers. The results revealed that most seeds germinated at 25 and30 °C, producing 83 and 84% of germination, higher germination speed (10,0 at25 °C and 10.7 at30 °C), and the greatest seedlings (10.3 mm at25 °C and10.8 mm at30 °C). Therefore, the best temperatures to germination are between 25 and30 °C.Keywords: Pantanal; forest seeds; seedlings formation; Salicaceae.

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Seed germination test for toxicity evaluation of compost: Its roles, problems and prospects

Waste Management ◽

10.1016/j.wasman.2017.09.023 ◽

2018 ◽

Vol 71 ◽

pp. 109-114 ◽

Cited By ~ 76

Author(s):

Yuan Luo ◽

Jie Liang ◽

Guangming Zeng ◽

Ming Chen ◽

Dan Mo ◽

...

Keyword(s):

Seed Germination ◽

Toxicity Evaluation ◽

Germination Test ◽

Seed Germination Test

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An instrumental set up for seed germination studies with temperature control and automatic image recording

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202007000200002 ◽

2007 ◽

Vol 19 (2) ◽

pp. 99-108 ◽

Cited By ~ 5

Author(s):

Pedro C.N. Teixeira ◽

José A. Coelho Neto ◽

Humberto Rocha ◽

Juracy M. de Oliveira

Keyword(s):

Seed Germination ◽

Forest Restoration ◽

Time Intervals ◽

Image Recording ◽

Germination Process ◽

Different Temperatures ◽

Set Up ◽

Germinating Seeds ◽

Flat Bed Scanner ◽

Predetermined Time

This paper describes a new instrumental methodology proposed for the study of seed germination which can be applied to germination physiology, agriculture and forest restoration. This instrument performs the recording of images of germinating seeds through the use of a flat-bed scanner coupled to a computer, where a specific program commands the capture of these images at predetermined time intervals. These images are archived for posterior analysis. The device allows the collection of data and the elaboration of germination curves with high accuracy. Furthermore, the device permits the study of seed germination over a broad range of temperatures as well as the easy application of chemical agents to the seeds in order to study their influence on the germination process. Lactuca sativa seeds were used in this study as an experimental model. Germination curves were plotted at different temperatures in order to evaluate the device for experimental work. This paper also presents a methodology of statistical analysis that supports the reliability of this equipment.

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