scholarly journals In vitro trypanocidal activity of solamargine and extracts from Solanum palinacanthum and Solanum lycocarpum of brazilian cerrado

2013 ◽  
Vol 85 (3) ◽  
pp. 903-907 ◽  
Author(s):  
RAQUEL R.D. MOREIRA ◽  
GILMARCIO Z. MARTINS ◽  
NATHALIA O. MAGALHAES ◽  
ADELIA E. ALMEIDA ◽  
ROSEMEIRE C.L.R. PIETRO ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Colorimetric Assay ◽  
Ethanolic Extract ◽  
Brazilian Cerrado ◽  
Strong Activity ◽  
Trypanocidal Activity ◽  
Mtt Colorimetric Assay ◽  
Ethanol Extracts ◽  
First Time

The present investigation was to evaluate the potential trypanocidal activity of crude ethanolic extract of the fruits of Solanum palinacanthum, Solanum lycocarpum and the glycoalcaloid, solamargine. S. palinacanthum and S. lycocarpum fruit powders were submitted to exhaustively extraction with 96% ethanol and solamargine were isolated from the extract of S. palinacanthum. Both extracts and solamargine were analysed for trypanocidal activity by using MTT colorimetric assay. Extracts of S. palinacanthum showed to be more active (IC50 = 175.9 µg.ml–1) than S. lycocarpum (IC50 = 194.7 µg.ml–1). Solamargine presented a strong activity (IC50 = 15.3 µg.ml–1), which can explain the better activity of the both extracts. Benznidazol (IC50 = 9.0 µg.ml–1) is the only drug used to treat Chagas' disease. These findings demonstrate for the first time that ethanol extracts obtained from both fruits of S. palinacanthum and S. lycocarpum and also solamargine have a potential anti-trypanosomal activity.

Evaluation of the in vitro trypanocidal activity of plant extracts from the Brazilian Cerrado

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WR Cunha ◽  
FM Dos Santos ◽  
JA Peixoto ◽  
RCS Veneziani ◽  
AEM Crotti ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Brazilian Cerrado ◽  
Trypanocidal Activity

Exploration of the in vitro cytotoxic and antiviral activities of different medicinal plants against infectious bursal disease (IBD) virus

2014 ◽  
Vol 9 (5) ◽  
pp. 531-542 ◽  
Author(s):  
Waqas Ahmad ◽  
Sohail Ejaz ◽  
Khaleeq Anwar ◽  
Muhammad Ashraf
Keyword(s):  
Medicinal Plants ◽  
Colorimetric Assay ◽  
Rna Virus ◽  
Infectious Bursal Disease ◽  
Dna Viruses ◽  
Mtt Colorimetric Assay ◽  
Bursal Disease ◽  
Dried Fruit ◽  
Antiviral Activities

AbstractInfectious bursal disease (IBD) caused by non-enveloped double stranded RNA virus is an acute and contagious poultry disease. Outbreak of IBD could result in 10–75% mortality of the birds; hence it has gained socio-economic importance worldwide. Medicinal plants have shown broad spectrum anti-viral activities against RNA and DNA viruses. Moringa oleifera Lam (MOL), Phyllanthus emblicus Linn (PEL), Glycyrrhiza glabra Linn (GGL), and Eugenia jambolana Lam (EJL) are commonly available medicinal plants of the sub-continent and exhibited anti-viral potential against different viruses. Ethanolic extracts of the leaves of MOL and EJL, roots of GGL and dried fruit of PEL were investigated for their cytotoxic and anti-viral potential against IBD virus using MTT colorimetric assay and anti-viral assay. Significant anti-viral potential (P<0.001) was demonstrated at concentrations 12.5, 25, 50 and 100 µg ml−1 of GGL, PEL, MOL and EJL, respectively, with no cytotoxicity. Data also spotlighted that all tested plant extracts possess significant anti-viral potential and this trend was higher in GGL followed by PEL, MOL, and EJL. The data undoubtedly conclude that these medicinal plants contain several health beneficial phyto-chemicals which got significant anti-viral potential and effectively be utilized against IBD virus. Moreover, the outcomes of this study provide a platform on the way to discover novel anti-viral agents against IBD virus and other viruses from plant origin.

scholarly journals In-vitro Control of Oral Thrush Causal Organisms Using Medicinal Plants Extracts

2019 ◽  
Vol 11 (3) ◽  
pp. 376-382
Author(s):  
Abosede M. EBABHI ◽  
Adedotun A. ADEKUNLE ◽  
Yoma I. OGHENERHABOKE
Keyword(s):  
Medicinal Plants ◽  
Jatropha Curcas ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Zone Of Inhibition ◽  
Test Organisms ◽  
Zones Of Inhibition ◽  
Oral Thrush ◽  
Ethanol Extracts

Oral hygiene is important to the generality of the human healthcare system. For this, the antifungal activities of the aqueous and ethanol extracts of four medicinal plants of Jatropha curcas (stem), Eucaluptus golbulus (leaves), Vernonia amygdalina (stem) and Zanthoxylum zanthoxyloides (root) were carried out in vitro against three species of Candida associated with oral thrush namely C. albicans, C. glabrata, C. tropicalis using the disc diffusion agar assay. The zones of inhibition varied with the test organisms as well as the extracts. The ethanolic extract of Jatropha curcas showed the highest zone of inhibition of 10.88 ± 0.22 mm against C. albicans while the least zone of inhibition (6.13 ± 0.13 mm) was exhibited by the ethanol extract of Z. zanthoxyloides on C. glabrata. The preliminary phytochemical screening showed the presence of tannin, saponin, alkaloids, flavonoids and reducing sugar in all plant samples. This study can be further used as a foundation for the screening of phytochemical constituents by pharmaceuticals for the control and eradication of oral thrush.

scholarly journals Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

2019 ◽  
Vol 30 (3) ◽  
pp. 201-207 ◽  
Author(s):  
Francine Lorencetti-Silva ◽  
Priscilla Aparecida Tartari Pereira ◽  
Alyne Fávero Galvão Meirelles ◽  
Lúcia Helena Faccioli ◽  
Francisco Wanderley Garcia Paula-Silva
Keyword(s):  
Stem Cells ◽  
Prostaglandin E2 ◽  
Dental Pulp ◽  
Colorimetric Assay ◽  
Dental Pulp Stem Cells ◽  
Lipid Mediator ◽  
Water Emulsion ◽  
Mtt Colorimetric Assay ◽  
Oil In Water Emulsion

Abstract Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells.

scholarly journals Cytotoxic Activity of Lomatiol and 7-(3′-Hydroxymethyl-3′-methylallyloxy)coumarin

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100
Author(s):  
Serena Fiorito ◽  
Salvatore Genovese ◽  
Francesco Epifano ◽  
Veronique Mathieu ◽  
Robert Kiss ◽  
...  
Keyword(s):  
Structural Feature ◽  
Colorimetric Assay ◽  
Parent Compound ◽  
Side Chain ◽  
In Vitro Growth ◽  
Mtt Colorimetric Assay ◽  
Growth Inhibitory ◽  
Inhibitory Activities ◽  
Comparison Data

7-(3′-Hydroxymethyl-3′-methylallyloxy)coumarin, bearing a 3′-hydroxy-3′-methylallyl group as the O-side chain, and lomatiol, a lapachol derivative sharing the same structural feature, were tested for their in vitro growth inhibitory activities on six cancer cell lines using the MTT colorimetric assay, along with the respective 3′,3′-dimethylallyl derivatives and unprenylated products used for comparison. Data revealed that lomatiol displayed the strongest growth inhibitory activities in vitro although not as efficient as the parent compound lapachol. The oxidized O-prenylcoumarin recorded better growth inhibitory capacities than the prenylated and unprenylated parent products.

In Vitro Assessment of Antibacterial Activity and Cytotoxicity of Silver Contained Antibacterial HA Coating Material

2009 ◽  
Vol 620-622 ◽  
pp. 307-310 ◽  
Author(s):  
Zi Yuan Zhu ◽  
Fu Qiang Zhang ◽  
You Tao Xie ◽  
Yi Kai Chen ◽  
Xue Bin Zheng
Keyword(s):  
Antibacterial Activity ◽  
Zirconium Phosphate ◽  
Pathogenic Bacteria ◽  
Colorimetric Assay ◽  
Vacuum Plasma Spraying ◽  
Vacuum Plasma ◽  
Mtt Colorimetric Assay ◽  
Ha Coating ◽  
Covering Method

Failure of dental implant is often caused by specific pathogenic bacteria. In this paper, antibacterial HA coating was prepared by vacuum plasma spraying with silver loaded zirconium phosphate containing HA as feedstock. The antibacterial activity against Aa，Fn and Pg was evaluated using a film covering method. MTT colorimetric assay was used to measure cytotoxic effect of the antibacterial HA coatings. Results show that the HA coatings displayed significant antibacterial activity against Aa，Fn, Pg and no cytotoxicity to L929 murine cell lines when the content of silver-loaded zirconium phosphate antimicrobial is between 5wt% to 10wt%. The sensitivity of the bacteria to the antibacterial HA coating is in sequence of Pg >Fn >Aa.

scholarly journals A New Xanthone from the Pericarp of Garcinia mangostana

2013 ◽  
Vol 8 (12) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Manqin Fu ◽  
Samuel X. Qiu ◽  
Yujuan Xu ◽  
Jijun Wu ◽  
Yulong Chen ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Human Cancer ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Hep G2 ◽  
Garcinia Mangostana ◽  
Human Cancer Cell Lines ◽  
Mtt Colorimetric Assay ◽  
Mcf 7

A new prenylxanthone, garcimangostanol (1), was isolated from the EtOAc-soluble partition of the ethanol extract of the pericarp of Garcinia mangostana L., along with three known compounds, namely 8-deoxygartanin (2), 1-isomangostin (3), and garcinone C (4). The structure of compound 1 was elucidated on the basis of its 1D, 2D NMR and MS data. Compounds 1–4 exhibited either significant or moderate cytotoxicity against MCF-7, A549, Hep-G2 and CNE human cancer cell lines in vitro with IC50 values from 4.0 ± 0.3 to 23.6 ± 1.5 μM by MTT colorimetric assay.

scholarly journals In Vitro Anti-proliferative Effect of Naturally Occurring Oxyprenylated Chalcones

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Serena Fiorito ◽  
Francesco Epifano ◽  
Celine Bruyère ◽  
Robert Kiss ◽  
Salvatore Genovese
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Mechanism Of Action ◽  
Colorimetric Assay ◽  
Appreciable Effect ◽  
Proliferative Effect ◽  
Mtt Colorimetric Assay ◽  
Naturally Occurring

As a continuation of our ongoing studies aimed to depict the effects and mechanism of action of naturally occurring oxyprenylated phenylpropanoids and polyketides, in this paper we describe the synthesis and in vitro anti-proliferative effects of selected compounds belonging to the above cited classes of secondary metabolites on six cancer cell lines using the MTT colorimetric assay. Our study revealed that among the natural products tested, only oxyprenylated chalcones exhibited an appreciable effect (mean IC50 = 32 - 64 μM), while substituted alcohols, phenylpropenes, naphthoquinones, and aminoacid derivatives were by far less active or inactive.

scholarly journals Synthesis and “in Vitro” Trypanocidal Activity Evaluation of Some Organo-iron Compounds.

2002 ◽  
Vol 8 (6) ◽  
pp. 329-332 ◽  
Author(s):  
Máfircio L. A. e Silva ◽  
Alberto F. Neto ◽  
Silvia A. Cardoso ◽  
Sérgio Albuquerque ◽  
Joseph Miller
Keyword(s):  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
In Vitro Assay ◽  
In Vitro Assays ◽  
Ferrocene Derivatives ◽  
Iron Compounds ◽  
Vitro Assay ◽  
Trypanocidal Activity ◽  
Activity Evaluation

Eight organo-iron ferrocene derivatives and arenocenium salts were prepared and evaluated by “in vitro” assay against one strain of Trypanosoma cruzi (Y). Six of the eight organo-iron compounds assayed, piperazinium diferrocenoate 1, η6-(o-xylene)-η5-(cyclopentadienyl) Iron(II) hexafluorophosphate 3, η6 -(mesitylene)-η5 -(cyclopentadienyl) iron(II) hexafluorphosphate 5, η6 -(durene)-η5 -(cyclopentadienyl) iron(II) hexafluorphosphate 6, η6 -(ρ-chlorotoluene)-η5 -(cyclopentadienyl) Iron(II) hexafluorphosphate 7 and η6 -(chlorobenzene)-η5 -(cyclopentadienyl) iron(II) picrate 8 , were poorly active in the “in vitro” assays. Only two compounds 1,1'–(N-pyperidinocarbonyl) ferrocene 2(IC50=2.4    μg/mL) and η6-(o-xylene)-η5(cyclopentadienyl) iron(II) picrate 4(IC50=12.08    μg/mL), were more active. Thus, some of the compounds are promising to be used against Chagas' disease as a prophylactic agents.

scholarly journals Study of Antioxidant Activity of Stalk and Fruit of Solanum melongena L. (Solanaceae)

2019 ◽  
pp. 1-7
Author(s):  
Kady Diatta ◽  
William Diatta ◽  
Alioune Dior Fall ◽  
Serigne Ibra Mbacké Dieng ◽  
Amadou Ibrahima Mbaye ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Solanum Melongena ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
In Vivo Studies ◽  
Ethanol Extracts

Background : Nowadays with the appearance of diseases such as cancer, atherosclerosis, free radicals are often singled out. What motivates scientific research in natural antioxidants. Aim/Objective : The aim of this study was to determine the antioxidant activity of the stalks and the fruit of Solanum melongena L. Study Duration : The period of the study was done on 25th July, 2015 at the Department of Pharmacy, Faculty of Medecine, Pharmacy and Odontology, University of Dakar, Senegal. Methodology : Antioxidant activity was evaluated through two methods (DPPH and FRAP). Results : For the FRAP test, at the highest concentration (83.3 µg/ml) the aqueous extract of the fruit (0.90±0.08) has a higher reducing power compared to those of ethanol extracts from the fruit (0.77±0.41) and the stalk (0.85±0.004). These results remain inferior to that of tannic acid (0.95± 0.0005). The DPPH test reveals that the ethanolic extract of the fruit is more effective in reducing the free radical DPPH with an inhibitory concentration 50 (IC 50) equal to 3.37±0.03 μg / ml, followed by the ethanolic extract of the stalks (IC 50 = 4.46±0.24 μg / ml) and finally the aqueous extract of the fruit (IC50 = 9.6±0.026 μg / ml). Conclusion : These results make it possible to confirm the in vitro activity of the parts studied, but in vivo studies are necessary in order to know the acute and chronic toxicities. Finally, perform a bio-guided fractionation to determine the molecules responsible for the antioxidant activity.

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