scholarly journals Distinct features of Pepper yellow mosaic virus isolates from tomato and sweetpepper

2004 ◽  
Vol 29 (6) ◽  
pp. 663-667 ◽  
Author(s):  
Luis C. V. da Cunha ◽  
Renato de O. Resende ◽  
Tatsuya Nagata ◽  
Alice K. Inoue-Nagata
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Untranslated Region ◽  
Sweet Pepper ◽  
Yellow Mosaic Virus ◽  
Horticultural Crops ◽  
Virus Diversity ◽  
Potyvirus Species ◽  
Capsicum Spp ◽  
Distinct Features

Determination of virus diversity in the field is vital to support a sustainable breeding program for virus resistance of horticultural crops. The present study aimed to characterize four field potyvirus isolates found naturally infecting sweet pepper (Capsicum annuum) (Sa66 and Sa115) and tomato (Lycopersicon esculentum) (IAC3 and Sa21) plants. Their biological characteristics revealed differences among the isolates in their ability to infect distinct Capsicum spp. and tomato genotypes, and in the severity of symptoms caused by these isolates compared to the infection caused by an isolate of Pepper yellow mosaic virus (PepYMV). Absence of cross-reaction was found among the studied isolates with antiserum against Potato virus Y (PVY). However, all isolates reacted, at different intensities, with antiserum against PepYMV. All isolates showed high identity percentage (97 to 99%) of the amino acid sequence of the coat protein with PepYMV (accession AF348610) and low (69 to 80%) with other potyvirus species. The comparison of the 3' untranslated region also confirmed this finding with 97 to 98% identity with PepYMV, and of 47 to 71% with other potyviruses. The results showed that PepYMV isolates were easily differentiated from PVY by serology and that the host response of each isolate could be variable. In addition, the nucleotide sequence of the coat protein and 3' untranslated region was highly conserved among the isolates.

scholarly journals Sources of resistance against the Pepper yellow mosaic virus in chili pepper

2009 ◽  
Vol 27 (2) ◽  
pp. 196-201 ◽  
Author(s):  
Cíntia dos S Bento ◽  
Rosana Rodrigues ◽  
Francisco Murilo Zerbini Júnior ◽  
Cláudia P Sudré
Keyword(s):  
Mosaic Virus ◽  
Rating Scale ◽  
Sweet Pepper ◽  
Negative Control ◽  
Yellow Mosaic Virus ◽  
Sources Of Resistance ◽  
Visual Evaluation ◽  
Area Reduction ◽  
Randomized Design ◽  
Capsicum Spp

The Pepper yellow mosaic virus (PepYMV) naturally infects chili and sweet pepper, as well as tomato plants in Brazil, leading to severe losses. This work reports the reaction to the PepYMV of 127 Capsicum spp. accessions, aiming at identifying resistance sources useful in breeding programs. The experiment was carried out in a completely randomized design, with eight replications, in greenhouse conditions. Plants were protected with an insect-proof screen to avoid virus dissemination by aphids. Leaves of Nicotiana debneyi infected with the PepYMV were used as the inoculum source. Plants were inoculated with three to four fully expanded leaves. A second inoculation was done 48 hours later to avoid escapes. Only the youngest fully expanded leaf was inoculated. Two plants were inoculated only with buffer, as negative control. Symptoms were visually scored using a rating scale ranging from 1 (assymptomatic plants) to 5 (severe mosaic and leaf area reduction). Nine accessions were found to be resistant based on visual evaluation. Their resistance was confirmed by ELISA. Two resistance accessions belong to the species C. baccatum var. pendulum, while the seven other were C. chinense. No resistant accessions were identified in C. annuum var. annuum, C. annuum var. glabriusculum, and C. frutescens.

Transformation of Wheat With the Gene Encoding the Coat Protein of Barley Yellow Mosaic Virus

1996 ◽  
Vol 23 (4) ◽  
pp. 429 ◽  
Author(s):  
S Karunaratne ◽  
A Sohn ◽  
A Mouradov ◽  
J Scott ◽  
HH Steinbiss ◽  
...  
Keyword(s):  
Gene Expression ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Bar Gene ◽  
Gene Encoding ◽  
Barley Yellow Mosaic Virus ◽  
Virus Coat Protein ◽  
Wheat Embryos ◽  
Virus Coat

Wheat plants (Triticum aestivum cv. Hartog) were stably transformed with the bar gene and the gene encoding the barley yellow mosaic virus coat protein. Cultured immature wheat embryos were bombarded with tungsten particles coated with the pEmuPAT-cp construct. Fifteen regenerating 'PPT- resistant' plants were selected on medium containing phosphinothricin. Of these, 11 plants had both the bar and cp genes integrated into the wheat genome and two plants had only the bar gene. Transmission of the two genes to progeny of two independent plants was confirmed. The barley yellow mosaic virus coat protein was detected in both the parent and progeny plants; however, bar gene expression occurred only in the parent plants.

Sequence diversity in the coat protein and 3?-untranslated region of Chinese yam necrotic mosaic virus RNA

2003 ◽  
Vol 69 (6) ◽  
pp. 397-399 ◽  
Author(s):  
Toru Kondo ◽  
Dong-Kyoon Kang ◽  
Shin-ichi Fuji ◽  
Moo-Ung Chang
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Untranslated Region ◽  
Sequence Diversity ◽  
Chinese Yam ◽  
Virus Rna

scholarly journals Role of Recombination in the Evolution of Host Specialization Within Bean yellow mosaic virus

2009 ◽  
Vol 99 (5) ◽  
pp. 512-518 ◽  
Author(s):  
S. J. Wylie ◽  
R. A. C. Jones
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Genetic Recombination ◽  
Bean Yellow Mosaic Virus ◽  
Host Specialization ◽  
Yellow Mosaic Virus ◽  
Complete Genomes ◽  
Tentative Evidence ◽  
Firm Evidence

Seven complete genomes and 64 coat protein gene sequences belonging to Bean yellow mosaic virus (BYMV) isolates from different continents were examined for evidence of genetic recombination using six different recombination-detection programs. In the seven complete genomes and a single complete genome of the related virus Clover yellow vein virus (ClYVV), evidence for eight recombination patterns was found by four or more programs, giving firm evidence of their presence, and five additional recombination patterns were detected by three or fewer programs, giving tentative evidence of their occurrence. When the nucleotide sequences of 64 BYMV and one ClYVV coat protein genes were analyzed, three firm recombination patterns were detected in 21 isolates (32%). With another six isolates (9%), tentative evidence was found for three further recombination patterns. Of the 19 firm or tentative recombination patterns detected within and between strain groups of BYMV, and with ClYVV, 12 involved a generalist group of isolates as a parent but none of the other BYMV groups acted as parents more than six times. These findings suggest that recombination played an important role in the evolution of BYMV strain groups that specialize in infecting particular groups of domesticated plants.

scholarly journals First Report of Bean yellow mosaic virus in Alaska from Clover (Trifolium spp.)

Plant Disease ◽  
2010 ◽  
Vol 94 (3) ◽  
pp. 372-372 ◽  
Author(s):  
N. L. Robertson ◽  
K. L. Brown
Keyword(s):  
Coat Protein ◽  
Host Range ◽  
Mosaic Virus ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Degenerate Primers ◽  
First Report ◽  
Chlorotic Spot ◽  
Putative Coat Protein ◽  
Das Elisa

In mid-June 2008, distinct mosaic leaves were observed on a cluster of clover (Trifolium spp.) with light pink and white flowers growing at the edge of a lawn in Palmer, AK. Virus minipurification from leaves of affected clover and protein extractions on a polyacrylamide electrophoresis implicated a ~35-kDa putative coat protein (CP). Subsequent western blots and ELISA with a universal potyvirus antiserum (Agdia Inc., Elkhart, IN) confirmed potyvirus identity. Total RNA extracts (RNeasy Plant Mini Kit, Qiagen Inc., Valencia, CA) from the same plant were used for reverse transcription (RT)-PCR. Three sets of degenerate primers that targeted potyvirus-specific genes, HC-Pro (helper component protease) and CI (cylindrical inclusion protein) and the genomic 3′-terminus that included a partial NIb (nuclear inclusion), CP (coat protein), and UTR (untranslated region), produced the expected PCR segments (~0.7, ~0.7, and ~1.6 kbp, respectively) on 1% agarose gels (1). Direct sequencing of the HC-Pro (GenBank No. GQ181115), CI (GQ181116), and CP (GU126690) segments revealed 98, 97, and 99% nucleotide identities (no gaps), respectively, to Bean yellow mosaic virus (BYMV)-chlorotic spot (CS) strain, GenBank No. AB373203. The next closest BYMV percent identity comparisons decreased to 79% for HC-Pro (GenBank No. DQ641248; BYMV-W), 79% for CI (U47033; BYMV-S) partial genes, and 96% for CP (AB041971; BYMV-P242). Mechanical inoculations of purified virus preparations produced local lesions on Chenopodium amaranticolor Coste & A. Reyn. (2 of 5) and C. quinoa Willd. (6 of 7), and mosaic on Nicotiana benthamiana Domin (5 of 5). BYMV was specifically confirmed on tester plants using a double-antibody sandwich (DAS)-ELISA BYMV (strain 204 and B25) kit (AC Diagnostics, Inc., Fayetteville, AR) as directed. The absence of another potyvirus commonly found in clover, Clover yellow vein virus (ClYVV), was verified in parallel DAS-ELISA ClYVV assays (AC Diagnostics, Inc). The BYMV isolate was maintained in N. benthamiana, and virion or sap extracts inoculated to the following host range (number of infected/total inoculated plants [verified by BYMV ELISA]): Cucumis sativus L. ‘Straight Eight’ (0/5), Gomphrena globosa L. (1/4), Nicotiana clevelandii A. Gray (4/7), Phaseolus vulgaris L. ‘Bountiful’ (1/3), Pisum sativum L. (Germplasm Resources Information Network Accession Nos. -PI 508092 (8/12), -W6 17525 (13/13), -W6 17529 (0/13), -W6 17530 (13/14), -W6 17537 (0/12), -W6 17538 (0/12), and -W6 17539 (0/21), Tetragonia tetragoniodes (2/2), Trifolium pretense L. ‘Altaswede’ (6/10), T. repens L. ‘Pilgrim’ (0/8), and Vicia faba L. (1/3). All infected plants had symptoms ranging from systemic mosaic (T. pretense, P. sativum) to leaf distortions (N. clevelandii, Tetragonia tetragoniodes). Interestingly, the host range and genomic sequences of the BYMV Alaskan strain resemble the BYMV-CS (chlorotic spot) strain that was originally isolated from a diseased red clover (T. pretense) plant in Japan more than 40 years ago (2). Although BYMV occurs worldwide and has a wide host range in dictoyledonous and monocotyledonous plants (3), to our knowledge, this is the first report of a natural occurrence of BYMV in Alaska. The incidence and distribution of BYMV in clover and other plant species are not known in Alaska. References: (1) C. Ha et al. Arch. Virol. 153:36, 2008. (2) H. Kume et al. Mem. Fac. Agric. Hokkaido Univ. 7:449, 1970. (3) S. J. Wylie et al. Plant Dis. 92:1596, 2008.

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