scholarly journals Establishment of anammox process in sludge samples collected from swine wastewater treatment system

2011 ◽  
Vol 31 (6) ◽  
pp. 1170-1178 ◽  
Author(s):  
Caroline G Casagrande ◽  
Airton Kunz ◽  
Hugo M Soares ◽  
Marina C. de Prá ◽  
Guilherme F Schierholt Neto
Keyword(s):  
Wastewater Treatment ◽  
Good Alternative ◽  
Effluent Treatment ◽  
Swine Wastewater ◽  
Aeration Tank ◽  
Biological Removal ◽  
Start Up ◽  
Anammox Activity ◽  
The Difference ◽  
Anammox Process

The high load of nitrogen present in swine wastewater is one of the biggest management challenges of the activity. The Anammox process emerges as a good alternative for biological removal of nitrogen. This study aims to acclimate sludge collected from swine effluent treatment systems to establish the Anammox process. Two sludge samples were collected at Embrapa Swine and Poultry, Concordia - SC, Brazil, one from the bottom of an inactive anaerobic pond (inoculum A) and another from an aeration tank (inoculum B). Both were acclimated until the depletion of NO3-N, being subsequently inoculated in two reactors (Reactor A - Inoculum A and Reactor B - Inoculum B). The Reactor A showed activity after 110 days of operation, while the Reactor B needed 170 days. The difference in the start-up time could be explained by the different environmental conditions to which each sludge was submitted. FISH and PCR analyses confirmed the presence of microorganisms with Anammox activity, demonstrating that the sludge of swine wastewater treatment systems is a good source of inoculum for the development of the Anammox process.

scholarly journals Achieving fast start-up of anammox process by promoting the growth of anammox bacteria with FeS addition

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Nitrite Reductase ◽  
Removal Rate ◽  
Anammox Bacteria ◽  
Gene Encoding ◽  
Start Up ◽  
Fast Start ◽  
The Difference ◽  
Anammox Process

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

Distribution and characterization of anammox in a swine wastewater activated sludge facility

2013 ◽  
Vol 67 (10) ◽  
pp. 2330-2336 ◽  
Author(s):  
Takao Yamagishi ◽  
Mio Takeuchi ◽  
Yuichiro Wakiya ◽  
Miyoko Waki
Keyword(s):  
Activated Sludge ◽  
Swine Wastewater ◽  
Assay Method ◽  
15N Tracer ◽  
Anammox Bacteria ◽  
Aeration Tank ◽  
Ammonium Oxidation ◽  
High Concentration ◽  
Anammox Activity ◽  
Biological Nitrogen

Anaerobic ammonium oxidation (anammox) is a novel biological nitrogen removal process that oxidizes NH4+ to N2 with NO2− as an electron acceptor. The purpose of this study was to examine the potential activity and characteristics of anammox in a conventional swine wastewater treatment facility, which uses an activated sludge system consisting of three cascade aeration tanks equipped with ceramic support material. Anammox activity was estimated by a 15N tracer assay method and was detected in all the sludge and biofilm samples in each aeration tank. Biofilm taken from the third aeration tank, in which the dissolved oxygen concentration was 7.5 mg/L and the wastewater included a high concentration of NO3−, showed by far the highest anammox activity. A clone library analysis showed the existence of anammox bacteria closely related to ‘Candidatus Jettenia asiatica’ and ‘Ca. Brocadia caroliniensis’. The optimum conditions for anammox activity were a pH of 6.7–7.2, a temperature of 35 °C, a NO2− concentration of 10 mmol/L or less, and an NH4+ concentration of 32 mmol/L or less.

scholarly journals Quick start-up of Mudanjiang wastewater treatment plant and factors influencing phosphorous removal

2013 ◽  
Vol 8 (1) ◽  
pp. 1-8
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Phosphorus Removal ◽  
Treatment Plant ◽  
Phosphorus Release ◽  
Aeration Tank ◽  
Phosphorous Removal ◽  
Start Up ◽  
The One

Successful start-up of a full-scale wastewater treatment plant (WWTP) is a key issue for the succeeding operation of WWTP on the one hand and the nutritious phosphorus removal is of great concern on the other. After the construction of Mudanjiang WWTP with a flow rate of 100,000 m3 d-1 in Heilongjiang Province of China, a novel way of start-up through feeding wastewater continuously into the system was attempted against the conventional start-up method of inoculating activated sludge in the aeration tank by feeding wastewater intermittently. Activated sludge was cultivated and proliferated in the aeration tanks instead of dosing acclimated sludge from other source. After one-month’s start-up operation, MLSS, SV and SVI increased to 2.5 kg m-3, 30% and nearly 80% respectively, which indicated that quick and simple start-up had been achieved. After successful start-up, an investigation into phosphorus removal was conducted with the emphasis on influencing factors such as ORP and NOx-N concentration etc. When the aeration tank was switched from aerobic to anaerobic mode, phosphorus removal efficiency of 80% could be realized within the whole treatment system. Experimental results revealed that an ORP of -140 mV and NOX-N of 2 mg l-1 were critical for the anaerobic phosphorus release, and DO in the range of 1.7-2.5 mg l-1, BOD5/TP of 20-30 and SVI of 70~80 as well as SRT of 5 days were the optimal phosphorus removal conditions for the aeration tanks.

scholarly journals Start-Up of Anammox SBR from Non-Specific Inoculum and Process Acceleration Methods by Hydrazine

Water ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 350
Author(s):  
Ivar Zekker ◽  
Oleg Artemchuk ◽  
Ergo Rikmann ◽  
Kelvin Ohimai ◽  
Gourav Dhar Bhowmick ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Biological Nutrient Removal ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Reject Water ◽  
Start Up ◽  
Anammox Activity ◽  
Optimum Salinity ◽  
Anammox Process

Biological nutrient removal from wastewater to reach acceptable levels is needed to protect water resources and avoid eutrophication. The start-up of an anaerobic ammonium oxidation (anammox) process from scratch was investigated in a 20 L sequence batch reactor (SBR) inoculated with a mixture of aerobic and anaerobic sludge at 30 ± 0.5 °C with a hydraulic retention time (HRT) of 2–3 days. The use of NH4Cl, NaNO2, and reject water as nitrogen sources created different salinity periods, in which the anammox process performance was assessed: low (<0.2 g of Cl−/L), high (18.2 g of Cl−/L), or optimum salinity (0.5–2 g of Cl−/L). Reject water feeding gave the optimum salinity, with an average nitrogen removal efficiency of 80%, and a TNRR of 0.08 kg N/m3/d being achieved after 193 days. The main aim was to show the effect of a hydrazine addition on the specific anammox activity (SAA) and denitrification activity in the start-up process to boost the autotrophic nitrogen removal from scratch. The effect of the anammox intermediate hydrazine addition was tested to assess its concentration effect (range of 2–12.5 mg of N2H4/L) on diminishing denitrifier activity and accelerating anammox activity at the same time. Heterotrophic denitrifiers’ activity was diminished by all hydrazine additions compared to the control; 5 mg of N2H4/L added enhanced SAA compared to the control, achieving an SAA of 0.72 (±0.01) mg N/g MLSS/h, while the test with 7.5 mg of N2H4/L reached the highest overall SAA of 0.98 (±0.09) mg N g/MLSS/h. The addition of trace amounts of hydrazine for 6 h was also able to enhance SAA after inhibition by organic carbon source sodium acetate addition at a high C/N ratio of 10/1. The start-up of anammox bacteria from the aerobic–anaerobic suspended biomass was successful, with hydrazine significantly accelerating anammox activity and decreasing denitrifier activity, making the method applicable for side-stream as well as mainstream treatment.

Sinks and sources of anammox bacteria in a wastewater treatment plant – screening with qPCR

2018 ◽  
Vol 78 (2) ◽  
pp. 441-451
Author(s):  
Linda Kanders ◽  
Maike Beier ◽  
Regina Nogueira ◽  
Emma Nehrenheim
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Quantitative Polymerase Chain Reaction ◽  
Treatment Plant ◽  
Anammox Bacteria ◽  
Reject Water ◽  
Start Up ◽  
Reliable Source ◽  
Polymerase Chain ◽  
Anammox Process

Abstract The deammonification process, which includes nitritation and anammox bacteria, is an energy-efficient nitrogen removal process. Starting up an anammox process in a wastewater treatment plant (WWTP) is still widely believed to require external seeding of anammox bacteria. To demonstrate the principle of a non-seeded anammox start-up, anammox bacteria in potential sources must be quantified. In this study, seven digesters, their substrates and reject water were sampled and quantitative polymerase chain reaction (qPCR) was used to quantify both total and viable anammox bacteria. The results show that mesophilic digesters fed with nitrifying sludge (with high sludge ages) can be classified as a reliable source of anammox bacteria. Sludge hygienization and dewatering of digestate reduce the amount of anammox bacteria by one to two orders of magnitude and can be considered as a sink. The sampled reject waters contained on average &gt;4.0 × 104 copies mL−1 and the majority of these cells (&gt;87%) were viable cells. Furthermore, plants with side-stream anammox treatment appear to have higher overall quantities of anammox bacteria than those without such treatment. The present study contributes to the development of sustainable strategies for both start-up of anammox reactors and the possibility of improving microbial management in WWTPs.

scholarly journals An Analysis of Operation Conditions and Microbial Characteristics in Swine Wastewater Treatment Plants with Spontaneously Enriched Anammox Bacteria

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1010
Author(s):  
Miyoko Waki ◽  
Chikako Ishimoto ◽  
Ryu Suto ◽  
Takafumi Nagamine ◽  
Toshimi Matsumoto ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Swine Wastewater ◽  
Continuous Reactor ◽  
Anammox Bacteria ◽  
Activity Levels ◽  
Operation Conditions ◽  
Microbial Characteristics ◽  
Anammox Activity ◽  
Treatment Facilities

The spontaneous enrichment of anammox bacteria has been reported in swine wastewater treatment facilities. However, their causative conditions and microbial characteristics, which this study aims to explain, are poorly understood. We discovered eight treatment facilities where the collected red biofilms exhibited high anammox activity levels at 57–843 µmol-N2/g-ignition loss (IL)/h and anammox DNA concentrations of 4.3 × 108–1.6 × 1012 copies/g-IL. The facilities used various wastewater treatment methods—six of them employed a multi-stage continuous reactor, whereas aeration tanks were continuously aerated at another combination of six facilities. Levels of dissolved oxygen (DO) in these tanks were fairly low at ≤1 mg/L. Pyrosequencing of the biofilms indicated the presence of 3–62.5% Planctomycetes, and the dominant anammox in each biofilm comprised three operational taxonomic units (OTUs) similar to Candidatus Jettenia asiatica, Ca. Brocadia fulgida, and Ca. B. caroliniensis. This suggested that some particular species of anammox bacteria naturally thrive when operating a swine wastewater treatment facility at low DO levels. The frequent enrichment of anammox biofilms at the sampled sites indicated that these treatment facilities were good seed sources of anammox; therefore, anammox treatment would be a viable method for the removal of nitrogen from swine wastewater.

scholarly journals ANÁLISE COMPARATIVA ENTRE ESTAÇÕES COMPACTAS E CONVENCIONAIS PARA O TRATAMENTO DE EFLUENTES NO MUNICÍPIO DE DOIS IRMÃOS, RS

2019 ◽  
Vol 10 (2) ◽  
pp. 121
Author(s):  
Cássio Rodrigo Dias Gomes ◽  
Éverton Hansen
Keyword(s):  
Wastewater Treatment ◽  
Conventional Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Rio Grande ◽  
Effluent Treatment ◽  
Septic Tank ◽  
Rio Grande Do Sul ◽  
The Difference

No Brasil, apenas uma pequena parcela do esgoto gerado pelas cidades é devidamente tratado em estações de tratamento de efluentes . O uso de soluções individuais (fossa séptica e sumidouro) é empregado em parte do esgoto gerado, e aproximadamente um terço do esgoto doméstico não possui coleta, tampouco tratamento. O presente estudo foi desenvolvido no município de Dois Irmãos, no Rio Grande do Sul. O município avaliado encontra-se em crescimento, com a implantação prevista de novos condomínios residenciais. Desta forma, o uso de estações compactas para o tratamento de efluentes líquidos foi avaliado, verificando sua eficiência e viabilidade econômica, em comparação com a tecnologia de fossa séptica e filtro anaeróbio coletivos, convencionalmente utilizada pelo município (estação de tratamento convencional). O trabalho mostra de forma comparativa, os custos de implantação, operação e manutenção, além de trazer as informações sobre a eficiência dos sistemas de tratamento de efluentes, quais sejam estação de tratamento compacta e estação de tratamento convencional. A questão mais relevante quando se compara os dois sistemas é a diferença no custo de implantação, onde o sistema compacto analisado seria até 81,22% mais econômico do que o convencional. Além da questão econômica, a eficiência do sistema compacto mostra-se superior ao sistema convencional. A estação compacta apresentou 97,97% de remoção de demanda bioquímica de oxigênio, enquanto a estação convencional removeu 77,67% do mesmo parâmetro. Palavras-chave: Estação de Tratamento de Efluente. ETE Compacta. ETE Convencional.ABSTRACTIn Brazil, only a small portion of the sewage generated by the cities is properly treated in wastewater treatment plants. The use of individual solutions (septic tank and sump) is used in part of the generated sewage, and approximately one-third of the domestic sewage has no collection or treatment. The present study was developed in the city of Dois Irmãos, Rio Grande do Sul. The evaluated municipality is growing, with the expected implementation of new residential condominiums. Thus, the use of compact stations for the treatment of liquid effluents was evaluated, verifying their efficiency and economic viability, in comparison with the technology of collective septic tank and anaerobic filter, conventionally used by the municipality (conventional treatment station). The work compares the implementation, operation and maintenance costs, as well as information on the efficiency of wastewater treatment systems (compact treatment plant and conventional treatment plant). The most relevant issue when comparing the two systems is the difference in deployment cost, where the compact system analyzed would be up to 81.22% more economical than the conventional one. Besides the economic issue, the efficiency of the compact system is superior to the conventional system. The compact station showed 97.97% of biochemical oxygen demand removal, while the conventional station removed 77.67% of the same parameter.Keywords: Effluent Treatment Station. Compact STE. Conventional STE.

Inhibition effect of swine wastewater heavy metals and antibiotics on anammox activity

2012 ◽  
Vol 66 (7) ◽  
pp. 1519-1526 ◽  
Author(s):  
T. Lotti ◽  
M. Cordola ◽  
R. Kleerebezem ◽  
S. Caffaz ◽  
C. Lubello ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Prolonged Exposure ◽  
Liquid Fraction ◽  
Swine Manure ◽  
Swine Wastewater ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Batch Tests ◽  
Anammox Activity ◽  
Anammox Process

The feasibility of anaerobic ammonium oxidation (anammox) process to treat wastewaters containing antibiotics and heavy metals (such as the liquid fraction of the anaerobically digested swine manure) was studied in this work. The specific anammox activity (SAA) was evaluated by means of manometric batch tests. The effects of oxytetracycline, sulfathiazole, copper and zinc were studied. The experimental data of the short-term assays were fitted with an inhibition model to identify the half maximal inhibitory concentration (IC50). After 24 h exposures, IC50-values equal to 1.9, 3.9, 650 and 1,100 mg L−1 were identified for copper, zinc, sulfathiazole and tetracycline respectively. The effect of prolonged exposure (14 days) to oxytetracycline and sulfathiazole was studied by means of repeated batch-assays. Anabolism and catabolism reactions were active during the inhibition tests indicating that anammox bacteria could grow even in the extreme conditions tested. Considering the average concentrations expected in swine wastewaters, the inhibitors studied do not seem to represent a problem for the application of the anammox process. However, in order to verify the effect of these compounds on the growth of anammox bacteria, continuous culture experiments could be conducted.

Sign in / Sign up

Export Citation Format

Share Document