scholarly journals Infecções do trato urinário (ITU) de cães e gatos: etiologia e resistência aos antimicrobianos

2014 ◽  
Vol 34 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Vania M. Carvalho ◽  
Tatiana Spinola ◽  
Fabrizia Tavolari ◽  
Kinue Irino ◽  
Rosana M. Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

As infecções bacterianas do trato urinário (ITUs) são causa comum de doença em cães, gatos e humanos. Embora bactérias Gram positivas como Staphylococcus spp., Streptococcus spp. e Enterococcus spp., possam ocasionar ITUs, as bactérias Gram negativas (Escherichia coli, Proteus spp., Klebsiella spp., Pseudomonas spp. e Enterobacter spp.) respondem por 75% dos casos. Este estudo teve como objetivo determinar a frequência de diferentes gêneros de bactérias em ITUs em cães e gatos, bem como a sua sensibilidade aos antimicrobianos utilizados na rotina clínica. Portanto, amostras de urina de 100 cães e gatos com sinais de ITU foram coletadas assepticamente, sofrendo avaliação microbiológica por meio de métodos qualitativos e quantitativos, além de urinálise. Todos os isolados foram submetidos a testes de sensibilidade aos antimicrobianos. ITU foi confirmada em 74% dos animais, não havendo predominância quanto ao sexo. No que diz respeito à idade, 85% dos cães e 87% dos gatos tinham idades superiores a seis anos. Noventa e cinco cepas bacterianas foram isoladas, com maior frequência de Escherichia coli (55% do total) dos sorogrupos O6 e O2. Constatou-se níveis elevados de resistência a antimicrobianos nas cepas isoladas. Para as cepas Gram positivas, tetraciclina (46,1%), enrofloxacina, cotrimazol e estreptomicina (42,3% cada) foram as drogas com os maiores índices de resistência. Para as Gram negativas, amoxacilina e tetraciclina apresentaram percentuais acima de 50%. Multiresistência foi verificada em mais de 50% dos principais gêneros isolados. Considerando-se que as cepas de E. coli apresentam potencial zoonótico e forte participação na disseminação de resistência aos antimicrobianos, ressalta-se a importância do papel do médico veterinário na prevenção e controle das ITUs animais e sua contribuição para a saúde pública.

PREVALENCIA DE INFECCIÓN URINARIA, UROPATÓGENOS Y PERFIL DE SUSCEPTIBILIDAD ANTIMICROBIANA, MEDELLÍN 2011-2012

2014 ◽  
Vol 39 (4) ◽  
pp. 352-358
Author(s):  
Jaiberth Antonio Cardona Arias ◽  
Claudia Patricia Orrego Marin ◽  
Claudia Patricia Henao Mejia
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Introducción: Las infecciones del tracto urinario (ITU), constituyen una de las infecciones bacterianas más prevalentes, sus agentes etiológicos incluyen Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones. Objetivo: Determinar la prevalencia de ITU, uropatógenos y el perfil de susceptibilidad antimicrobiana en una Institución Prestadora de Servicios de Salud (IPS) de Medellín, 2011-2012. Métodos: Estudio de prevalencia en 1.959 individuos atendidos en una IPS de tercer nivel. Se calcularon medidas de resumen, proporciones, razones de prevalencia, Chi cuadrado y Fisher. Se cuantificó la modificación del efecto (confusión o interacción) con análisis estratificado y modelos de regresión logística binaria en SPSS 21.0®. Resultados: La prevalencia de ITU fue 31% los principales agentes etiológicos fueron E. coli (69%), Enterococcus spp (11%) y Klebsiella spp (8%). La ITU y la infección por E. coli fueron estadísticamente mayores en mujeres y adultos mayores. La mayor frecuencia de resistencia de E. coli fue para ampicilina (61%), Ácido nalidixico (48%), TrimetropinSulfa (48%) y Ciprofloxacina (42%); mientras que en Klebsiella spp fue Ampicilina (100%), TrimetropinSulfa (23%), Ampicilina- Sulbactam (22%) y Cefalotina (19%). Conclusión: La elevada prevalencia de ITU, la multiplicidad de uropatógenos aislados, la identificación de grupos de mayor riesgo y la diversidad de perfiles de resistencia antibiótica, evidencian la necesidad de desarrollar investigaciones locales que permitan orientar las acciones en salud y vigilancia epidemiológica, acordes con las particularidades de cada población.

scholarly journals Análisis epidemiológico de las infecciones urinarias en pacientes ambulatorios del Centro Médico Bautista

2020 ◽  
Vol 8 ◽  
pp. 109
Author(s):  
Lisa Ibarrola de Ávila ◽  
Lourdes Talavera ◽  
María Marta González ◽  
Fernando Ávila
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Las Mujeres ◽  
Staphylococcus Spp ◽  
Los Grupos ◽  
Klebsiella Spp

Las infecciones del tracto urinario son las infecciones más comunes en todo el mundo y se presentan en todos los grupos de población, se estima que entre el 40% y el 50% de las mujeres  presentarán al menos una durante su vida, y de estas el  11% tendrá una infección al año, siendo la causa más frecuente de origen bacteriano (80%-90%). Constituyen una causa frecuente de consulta en atención primaria, afecta mayoritariamente a las mujeres, generalmente son monobacterianas ,sus  agentes etiológicos incluyen :  Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones por lo que es relevante contar con esta  información para generar un perfil clínico-epidemiológico propio que permita la implementación de medidas terapéuticas correctas, así como  la atención y control de la resistencia bacteriana, acordes con las particularidades de la población del lugar de estudio.

Bakterienisolate aus dem unteren Respirationstrakt von erkrankten Hunden und deren aktuelle Resistenzsituation

2012 ◽  
Vol 40 (05) ◽  
pp. 309-317 ◽  
Author(s):  
E. Prenger-Berninghoff ◽  
N. Bauer ◽  
R. Weiß ◽  
A. Moritz ◽  
A. Steinfeld
Keyword(s):  
Escherichia Coli ◽  
Polymyxin B ◽  
Bordetella Bronchiseptica ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Klinische Relevanz ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Zusammenfassung Gegenstand: Retrospektive Untersuchung der mittels bronchoalveolärer Lavage (BAL) gewonnenen Keimflora der tiefen Atemwege erkrankter Hunde auf ihre Antibiotikaempfindlichkeit über 5 Jahre. Material und Methoden: Auswertung der Ergebnisse der Agardiffusionstests von Bakterienisolaten, die in den Jahren 2004–2009 von 84 Hunden isoliert wurden, und Vergleich mit den Resultaten einer gleichartigen Analyse der Jahre 1999/2000. Ergebnisse: Bei den 99 geprüften Bakterienisolaten handelte es sich um Pasteurella spp. (27,3%), Bordetella bronchiseptica (20,2%), Staphylococcus spp. (18,2%), Escherichia coli (15,2%), Klebsiella spp. (8,1%), Pseudomonas spp. (7,0%) und Streptococcus spp. (4,0%). Bei acht Hunden lagen Mischkulturen vor. Die Mehrzahl der Bordetella-(B.-)bronchiseptica-Isolate erwies sich als sensibel gegenüber den Fluorchinolonen sowie Tetracyclin, Doxycyclin und Polymyxin B. Im Vergleich zu 1999/2000 zeigte sich ein höherer Anteil der gegenüber Amoxicillin/Clavulansäure und Chloramphenicol empfindlichen B.-bronchiseptica-Isolate, wobei die Zunahme bezüglich Chloramphenicol statistisch signifikant war. Die überwiegende Anzahl der Staphylococcus-Isolate erwies sich gegenüber Enrofloxacin, Marbofloxacin, Amoxicillin/Clavulansäure, Chloramphenicol, Cephalexin, Doxycyclin und Polymyxin B sensibel. Im Vergleich zu 1999/2000 ergab sich ein erhöhter Anteil an Chloramphenicolund Tetracyclin-sensiblen Staphylococcus-Isolaten. Der Anteil Enrofloxacin-sensibler KlebsiellenIsolate betrug 62,5%, während er 1999/2000 bei 100% lag. Eine Sensibilität aller getesteten Klebsiellen-Isolate ließ sich noch gegenüber Polymyxin B nachweisen. Kein Antibiotikum zeigte eine Wirksamkeit gegenüber allen E.-coli-Isolaten. Statistisch signifikant war die Abnahme der gegenüber Amoxicillin/Clavulansäure-sensiblen Isolate. Schlussfolgerung und klinische Relevanz: Die Studie unterstreicht den Nutzen der BAL für eine ätiologische Diagnostik und den anschließenden fundierten Einsatz von Antibiotika. Die Mehrzahl der Bakterienisolate erwies sich als empfindlich gegenüber den Fluorchinolonen.

scholarly journals Prevalence of bovine mastitis pathogens and antibioresistance of Escherichia coli isolates

2017 ◽  
Vol 55 (2) ◽  
pp. 113
Author(s):  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
P. TSAKOS (Π. ΤΣΑΚΟΣ) ◽  
K. ANATOLIOTIS (Κ. ΑΝΑΤΟΛΙΩΤΗΣ)
Keyword(s):  
Escherichia Coli ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Resistance Rate ◽  
Northern Greece ◽  
E Coli ◽  
Milk Samples ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Mastitis Pathogens

Nine hundred and fifty two milk samples from clinical bovine mastitis cases, originated from 269 farms in Northern Greece, were tested. Escherichia coli was isolated in 49.3% of samples. Furthermore, Staphylococcus spp, Corynebacterium spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Klebsiella spp or a combination of the above bacteria were isolated. The bacteriological result from 4.3% of samples was negative. No correlation between clinical mastitis cases and seasonal variation was observed. Resistance of E. coli isolates to tetracycline was 82-96%, to enrofloxacin 10-30%, to gentamicin 58-80%, to cephalosporins 47-75%, to sulfamethoxazole-trimethoprime 46-81%, to ampicillin 71-92% and to neomycin 83-97%. The highest resistance rate and the appearance of multi-resistant isolates of E. coli (6%), to 8 antibacterials, were recorded during the last year of the survey.

scholarly journals Prevalencia y Patrones de Sensibilidad de Microorganismos Aislados en Celulares y Estetoscopios de Estudiantes de Medicina de Pregrado y Posgrado Rotando en un Hospital de 4 Nivel en Bogotá, D.C

2017 ◽  
Vol 23 (1) ◽  
pp. 10 ◽  
Author(s):  
Juan Sebastián Herrera Rodríguez ◽  
Jesica Tatiana Muñóz Romero ◽  
Carlos Andrés Botero García ◽  
Iván Alberto Méndez Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Acinetobacter Spp ◽  
Para Determinar ◽  
Klebsiella Spp ◽  
De Medicina

Introducción: Los dispositivos médicos como el fonendoscopio, son manipulados constantemente en el entorno del paciente. El auge que han tenido los teléfonos celulares en la actualidad, los ha convertido en un objeto de uso común inclusive para el personal de atención en salud Objetivos: Identificar los microorganismos y su patrón de sensibilidad a antibióticos aislados de celulares y fonendoscopios de estudiantes de medicina de pregrado y postgrado. Metodología: Se tomaron por hisopado 77 muestras entre celulares y fonendoscopios (43/34) y se cultivaron en agar sangre a 37°C por 24-36 horas, se realizaron pruebas específicas para determinar el microorganismo y el patrón de sensibilidad. Resultados: De 43 muestras en celulares se aislaron 92 microorganismos principalmente  Staphylococcus coagulasa negativo (SCN) (51%), Staphylococcus aureus meticilino sensible (SAMS) (14%), Escherichia coli (5%), Klebsiella spp (3%), Acinetobacter spp (2%), Pseudomonas spp (2%), Staphylococcus aureus meticilino resistente (SAMR) (2%). De 34 muestras de fonendosocopios se hallaron 59 microorganismos distribuidos así: SCN (61%), SAMS (15%), SAMR (5%), Pseudomonas spp (3%), Klebsiella spp (3%), E. coli (3%), Acinetobacter spp (2%). El 25% y 13% de los Staphylococcus aureus en fonendos y celulares fueron SAMR, en cuanto a los bacilos Gram negativos en fonendoscopios, la mayoría mostró sensibilidad a los antibióticos empleados a excepción de algunas cepas de Klebsiella spp, Acinetobacter spp y Escherichia coli. En celulares el patrón de sensibilidad fue más heterogéneo, destacando que el 50% de las cepas de Pseudomonas spp fueron resistentes a la Ceftriaxona y el 100% de las cepas de Acinetobacter spp a los β-lactámicos probados. Conclusiones: La colonización de dispositivos médicos y electrónicos por microorganismos patógenos resistentes a diferentes familias de antimicrobianos puede presentar un riesgo de transmisión a pacientes hospitalarios y ambulatorios al exponerlos al contacto con estos.

scholarly journals ПОКАЗНИКИ АНТИЛІЗОЦИМНОЇ ТА АНТИКОМПЛЕМЕНТАРНОЇ АКТИВНОСТІ МІКРООРГАНІЗМІВ ДИСТАЛЬНОГО ВІДДІЛУ КИШЕЧНИКА ПОРОСЯТ ЗА ЗМІШАНОЇ НЕМАТОДОЗНО-ПРОТОЗООЗНОЇ ІНВАЗІЇ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 248-251
Author(s):  
R.A. Peleno
Keyword(s):  
Intestinal Parasites ◽  
Anticomplementary Activity ◽  
Candida Spp ◽  
Distal Intestine ◽  
E Coli ◽  
Vital Functions ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Lactobacillus Spp

The article deals with the results of search of the influence of intestinal parasites on the indicators of antilysozyme and anticomplementary activity of microorganism of distal department of intestine. It was established, that the products of intestinal parasites of pigs reduce anti lysozyme activity of such obligate anaerobes as Bifidobacterium spp. to 42.1% (р < 0.01), Prevotella spp. to 14.3%, Clostridium spр. to 66.7% (р < 0,001). With MAFAnM to 38.5% (р < 0.05) in Lactobacillus spp. and to 50.0% (р < 0.01) in Streptococcus spp. Higher compared with the control, antilysozyme activity was in Bacteroides spp. (р < 0.05), Eubacterium spp. (р < 0,001), Propionіbacterium spp. (р < 0.01), Peptostreptococcus spp. (р < 0.05), Fusobacterium spp. (р < 0.01), E. coli lact. «+» hem «-» (р < 0.05), Klebsiella spp. (р < 0.001), Citrobacter spp. (р < 0.01), Enterococcus spp. (р < 0.05), Staphylococcus spp. (р<0.001), Candida spp. (р<0.001) abstracted from the distal intestine of pigs affected with mixed nematodosis - protoozic invasion. With the action of products of vital functions of ascarices, eymeria and balantides, the anticomplementary activity significantly increases in Prevotella spp. (р < 0.01), Clostridium spр. (р < 0.001), Eubacterium spp. (р < 0.001), Propionіbacterium spp. (р < 0.001), Peptostreptococcus spp. (р < 0,001), Fusobacterium spp. (р < 0.001), Lactobacillus spp. (р < 0.05), E. coli lact. «+» hem «-» (р < 0.01), E. coli lact. «-» hem «+» (р < 0.001), Klebsiella spp. (р < 0.05), Enterobacter spp. (р < 0.001), Enterococcus spp. (р < 0.001), Streptococcus spp. (р < 0.001) and only in Bacteroides spp. it was significantly lower (р < 0.001), compared with the control.

scholarly journals Isolation of Escherichia coli, Klebsiella spp. and Staphylococcus spp. from Bovine Mastitic Milk in Nuwera Eliya District of Sri Lanka and their Sensitivity to Commonly Used Antibiotics

2018 ◽  
pp. 1-13
Author(s):  
T. S. P. J. Jayaweera ◽  
J. L. P. C. Randika ◽  
H. G. C. L. Gamage ◽  
N. N. Udawatta ◽  
W. U. N. T. S. Ellepola ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sri Lanka ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Milk Samples ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Clinical Cases

Aims: Mastitis is one of the very important and most common diseases among dairy cattle globally which leads to severe economical losses in the dairy industry. For the sustainability of the dairy sector it is critical that efficient, economically feasible treatment regime is available for clinical cases of mastitis as a part of the control program with minimum risk for residues in milk. Antimicrobials are the most common drugs of choice for controlling and preventing this devastating condition. But the frequent use of antibiotics leads to the development of resistant bacteria which could have an adverse effect on human health as well. To mitigate this destructive constraint in the industry, identifying the etiology and their susceptibilities to remedial measures are of paramount importance. Hence this study was aimed at isolating and identifying the common bacterial etiology Escherichia coli, Klebsiella spp. and Staphylococcus spp. of mastitis and evaluating the antimicrobial susceptibility of the isolates in order to develop mastitis control strategies in the area. Study Design: Milk samples were collected from mastitic cows in different stages including subclinical and clinical cases based on the results of California Mastitis Test Place and Duration of Study: Samples were collected from dairy farms in Nuwera Eliya District, Sri Lanka and Laboratory investigations were carried out in the Laboratory of Livestock Production, Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanka, Between Aug. 2017 and Nov. 2017. Methodology: E.coli, Klebsiella spp. and Staphylococcus spp. were isolated from 31 milk samples and susceptibility to commonly used antibiotics (Trimethoprim, Oxytetracycline, Chloramphenicol, Cephalexin, Enrofloxacin and Ciprofloxacin) was determined by Kirby Bauer disk diffusion method. Results: The study revealed that the most common isolate was the Klebsiella spp. and it is 54.8% and other two organisms Staphylococcus spp. had 51.6% and Escherichia coli 41.9%. Of all isolated pathogen, 97.1% exhibited resistant to Cephalexin and it was the highest while lowest resistance was to Chloramphenicol (31.4%). Among the other antibiotics, 54.3% of total isolates showed resistance to Trimethoprim followed by 42.9% to Oxytetracycline and Enrofloxacin, 34.3% was resistant to Ciprofloxacin. Resistance to at least one antibiotic was observed for the isolated microorganisms. All the three isolated pathogens are more resistant to Cephalexin. Both E. coli and Klebsiella spp. show 100% resistance to Cephalexin while Staphylococci had 92.9% resistance. This further revealed that E. coli (10%) and Klebsiella spp. (27.3%) are showing the least resistance to Chloramphenicol, None of Staphylococcus spp. (0%) isolated show resistance to Enrofloxacin. Conclusion: Most common organisms isolated were Klebsiella spp. followed by Staphylococcus spp., E. coli and there is a resistance of isolated organisms to some commonly used antibiotics.

scholarly journals Pathogenic identification and antibiotic susceptibility of the microorganisms isolated from urine samples of UTI patients

2017 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Md Ibrahim Miah ◽  
Tasnia Ahmed ◽  
Saurab Kishore Munshi
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pathogenic Bacteria ◽  
Research Work ◽  
Ongoing Research ◽  
Pseudomonas Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Urinary tract infection (UTI) is the infection that occurs in the urinary tract by the invasion of pathogenic bacteria, fungi, virus etc. under some circumstances only. Some common pathogenic bacteria associated with UTI include Escherichia coli, Proteus spp., Streptococcus spp., Staphylococcus spp., Pseudomonas spp. In the ongoing research work, about 30 UTI patients were selected randomly to detect the pathogen responsible for causing urinary tract infection and also to demonstrate their drug resistant traits. Among the 30 samples, about 14 samples were found to harbor Proteus spp. (1×104 cfu/ml to 1×105 cfu/ml). Escherichia coli was found to be the second predominant agent for UTI and recovered from 13 samples. Among the rest 3 samples, Pseudomonas spp. was found in 2 samples and Klebsiella spp. was found in one sample. Identified isolates showed resistance against different ranges of antibiotics. The study findings revealed the fact that emerging drug resistance of different pathogenic bacteria could become the major difficulties in the treatment of infectious diseases like UTI.Stamford Journal of Microbiology, Vol.6(1) 2016: 34-38

scholarly journals Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Salmonella Spp ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

scholarly journals Antibacterial susceptability of E. coli strains isolated from raw milk

2021 ◽  
Vol 2 (3) ◽  
pp. 48-54
Author(s):  
Valerii USHKALOV ◽  
Vyacheslav DANCHUK ◽  
Artem USHKALOV ◽  
Aidyn SALMANOV ◽  
Yuriy VISHOVAN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Raw Milk ◽  
Farm Animals ◽  
Strategy Development ◽  
Diffusion Method ◽  
E Coli ◽  
Milk Samples ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Hygienic Conditions

ntroduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia coli, which developed resistance to antibacterial drugs.Material and methods. Raw milk samples were obtained from clinically healthy cows on farms from Kiev and Poltava regions to identify E . coli, Staphylococcus spp., Enterococcus spp. isolates. Antimicrobial susceptibility testing was performed using the EUCAST disk diffusion method and MU on “Determination of microbial susceptibility to antibacterial drugs”. Results. The examined milk samples revealed the presence of E . coli, Staphylococcus spp. and Enterococcus spp. isolates, which proves poor sanitary and hygienic conditions of milk production process. Escherichia coli isolates were found susceptible to Ampicillin/sulbactam, Cefoxitin (100%), Meropenem, Tobramycin (100%), Netilin, Tigecycline, Nitroxoline (100%), Gatifloxacin, and Nitrofurantoin (100%). The studied E. coli isolates were found resistant to Ampicillin (100%), Imipenem, Tetracycline, and Doxycycline (100%). 41.7% of isolates of Staphylococcus epidermidis, Staphylococcus aureus were found resistant to Oxacillin, of which 90% were resistant to Benzylpenicillin and 20% to Rifampicin. Conclusions.The circulation of antibiotic-resistant Enterobacteriaceae strains among farm animals is a major problem requiring a strategy development aimed to prevent the emergence and spread of antibiotic resistance worldwide.

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