Bakterienisolate aus dem unteren Respirationstrakt von erkrankten Hunden und deren aktuelle Resistenzsituation

2012 ◽  
Vol 40 (05) ◽  
pp. 309-317 ◽  
Author(s):  
E. Prenger-Berninghoff ◽  
N. Bauer ◽  
R. Weiß ◽  
A. Moritz ◽  
A. Steinfeld

Zusammenfassung Gegenstand: Retrospektive Untersuchung der mittels bronchoalveolärer Lavage (BAL) gewonnenen Keimflora der tiefen Atemwege erkrankter Hunde auf ihre Antibiotikaempfindlichkeit über 5 Jahre. Material und Methoden: Auswertung der Ergebnisse der Agardiffusionstests von Bakterienisolaten, die in den Jahren 2004–2009 von 84 Hunden isoliert wurden, und Vergleich mit den Resultaten einer gleichartigen Analyse der Jahre 1999/2000. Ergebnisse: Bei den 99 geprüften Bakterienisolaten handelte es sich um Pasteurella spp. (27,3%), Bordetella bronchiseptica (20,2%), Staphylococcus spp. (18,2%), Escherichia coli (15,2%), Klebsiella spp. (8,1%), Pseudomonas spp. (7,0%) und Streptococcus spp. (4,0%). Bei acht Hunden lagen Mischkulturen vor. Die Mehrzahl der Bordetella-(B.-)bronchiseptica-Isolate erwies sich als sensibel gegenüber den Fluorchinolonen sowie Tetracyclin, Doxycyclin und Polymyxin B. Im Vergleich zu 1999/2000 zeigte sich ein höherer Anteil der gegenüber Amoxicillin/Clavulansäure und Chloramphenicol empfindlichen B.-bronchiseptica-Isolate, wobei die Zunahme bezüglich Chloramphenicol statistisch signifikant war. Die überwiegende Anzahl der Staphylococcus-Isolate erwies sich gegenüber Enrofloxacin, Marbofloxacin, Amoxicillin/Clavulansäure, Chloramphenicol, Cephalexin, Doxycyclin und Polymyxin B sensibel. Im Vergleich zu 1999/2000 ergab sich ein erhöhter Anteil an Chloramphenicolund Tetracyclin-sensiblen Staphylococcus-Isolaten. Der Anteil Enrofloxacin-sensibler KlebsiellenIsolate betrug 62,5%, während er 1999/2000 bei 100% lag. Eine Sensibilität aller getesteten Klebsiellen-Isolate ließ sich noch gegenüber Polymyxin B nachweisen. Kein Antibiotikum zeigte eine Wirksamkeit gegenüber allen E.-coli-Isolaten. Statistisch signifikant war die Abnahme der gegenüber Amoxicillin/Clavulansäure-sensiblen Isolate. Schlussfolgerung und klinische Relevanz: Die Studie unterstreicht den Nutzen der BAL für eine ätiologische Diagnostik und den anschließenden fundierten Einsatz von Antibiotika. Die Mehrzahl der Bakterienisolate erwies sich als empfindlich gegenüber den Fluorchinolonen.

2014 ◽  
Vol 39 (4) ◽  
pp. 352-358
Author(s):  
Jaiberth Antonio Cardona Arias ◽  
Claudia Patricia Orrego Marin ◽  
Claudia Patricia Henao Mejia

Introducción: Las infecciones del tracto urinario (ITU), constituyen una de las infecciones bacterianas más prevalentes, sus agentes etiológicos incluyen Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones. Objetivo: Determinar la prevalencia de ITU, uropatógenos y el perfil de susceptibilidad antimicrobiana en una Institución Prestadora de Servicios de Salud (IPS) de Medellín, 2011-2012. Métodos: Estudio de prevalencia en 1.959 individuos atendidos en una IPS de tercer nivel. Se calcularon medidas de resumen, proporciones, razones de prevalencia, Chi cuadrado y Fisher. Se cuantificó la modificación del efecto (confusión o interacción) con análisis estratificado y modelos de regresión logística binaria en SPSS 21.0®. Resultados: La prevalencia de ITU fue 31% los principales agentes etiológicos fueron E. coli (69%), Enterococcus spp (11%) y Klebsiella spp (8%). La ITU y la infección por E. coli fueron estadísticamente mayores en mujeres y adultos mayores. La mayor frecuencia de resistencia de E. coli fue para ampicilina (61%), Ácido nalidixico (48%), TrimetropinSulfa (48%) y Ciprofloxacina (42%); mientras que en Klebsiella spp fue Ampicilina (100%), TrimetropinSulfa (23%), Ampicilina- Sulbactam (22%) y Cefalotina (19%). Conclusión: La elevada prevalencia de ITU, la multiplicidad de uropatógenos aislados, la identificación de grupos de mayor riesgo y la diversidad de perfiles de resistencia antibiótica, evidencian la necesidad de desarrollar investigaciones locales que permitan orientar las acciones en salud y vigilancia epidemiológica, acordes con las particularidades de cada población.


2014 ◽  
Vol 34 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Vania M. Carvalho ◽  
Tatiana Spinola ◽  
Fabrizia Tavolari ◽  
Kinue Irino ◽  
Rosana M. Oliveira ◽  
...  

As infecções bacterianas do trato urinário (ITUs) são causa comum de doença em cães, gatos e humanos. Embora bactérias Gram positivas como Staphylococcus spp., Streptococcus spp. e Enterococcus spp., possam ocasionar ITUs, as bactérias Gram negativas (Escherichia coli, Proteus spp., Klebsiella spp., Pseudomonas spp. e Enterobacter spp.) respondem por 75% dos casos. Este estudo teve como objetivo determinar a frequência de diferentes gêneros de bactérias em ITUs em cães e gatos, bem como a sua sensibilidade aos antimicrobianos utilizados na rotina clínica. Portanto, amostras de urina de 100 cães e gatos com sinais de ITU foram coletadas assepticamente, sofrendo avaliação microbiológica por meio de métodos qualitativos e quantitativos, além de urinálise. Todos os isolados foram submetidos a testes de sensibilidade aos antimicrobianos. ITU foi confirmada em 74% dos animais, não havendo predominância quanto ao sexo. No que diz respeito à idade, 85% dos cães e 87% dos gatos tinham idades superiores a seis anos. Noventa e cinco cepas bacterianas foram isoladas, com maior frequência de Escherichia coli (55% do total) dos sorogrupos O6 e O2. Constatou-se níveis elevados de resistência a antimicrobianos nas cepas isoladas. Para as cepas Gram positivas, tetraciclina (46,1%), enrofloxacina, cotrimazol e estreptomicina (42,3% cada) foram as drogas com os maiores índices de resistência. Para as Gram negativas, amoxacilina e tetraciclina apresentaram percentuais acima de 50%. Multiresistência foi verificada em mais de 50% dos principais gêneros isolados. Considerando-se que as cepas de E. coli apresentam potencial zoonótico e forte participação na disseminação de resistência aos antimicrobianos, ressalta-se a importância do papel do médico veterinário na prevenção e controle das ITUs animais e sua contribuição para a saúde pública.


2017 ◽  
Vol 55 (2) ◽  
pp. 113
Author(s):  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
P. TSAKOS (Π. ΤΣΑΚΟΣ) ◽  
K. ANATOLIOTIS (Κ. ΑΝΑΤΟΛΙΩΤΗΣ)

Nine hundred and fifty two milk samples from clinical bovine mastitis cases, originated from 269 farms in Northern Greece, were tested. Escherichia coli was isolated in 49.3% of samples. Furthermore, Staphylococcus spp, Corynebacterium spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Klebsiella spp or a combination of the above bacteria were isolated. The bacteriological result from 4.3% of samples was negative. No correlation between clinical mastitis cases and seasonal variation was observed. Resistance of E. coli isolates to tetracycline was 82-96%, to enrofloxacin 10-30%, to gentamicin 58-80%, to cephalosporins 47-75%, to sulfamethoxazole-trimethoprime 46-81%, to ampicillin 71-92% and to neomycin 83-97%. The highest resistance rate and the appearance of multi-resistant isolates of E. coli (6%), to 8 antibacterials, were recorded during the last year of the survey.


2020 ◽  
Vol 8 ◽  
pp. 109
Author(s):  
Lisa Ibarrola de Ávila ◽  
Lourdes Talavera ◽  
María Marta González ◽  
Fernando Ávila

Las infecciones del tracto urinario son las infecciones más comunes en todo el mundo y se presentan en todos los grupos de población, se estima que entre el 40% y el 50% de las mujeres  presentarán al menos una durante su vida, y de estas el  11% tendrá una infección al año, siendo la causa más frecuente de origen bacteriano (80%-90%). Constituyen una causa frecuente de consulta en atención primaria, afecta mayoritariamente a las mujeres, generalmente son monobacterianas ,sus  agentes etiológicos incluyen :  Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones por lo que es relevante contar con esta  información para generar un perfil clínico-epidemiológico propio que permita la implementación de medidas terapéuticas correctas, así como  la atención y control de la resistencia bacteriana, acordes con las particularidades de la población del lugar de estudio.


2017 ◽  
Vol 23 (1) ◽  
pp. 10 ◽  
Author(s):  
Juan Sebastián Herrera Rodríguez ◽  
Jesica Tatiana Muñóz Romero ◽  
Carlos Andrés Botero García ◽  
Iván Alberto Méndez Rodríguez

Introducción: Los dispositivos médicos como el fonendoscopio, son manipulados constantemente en el entorno del paciente. El auge que han tenido los teléfonos celulares en la actualidad, los ha convertido en un objeto de uso común inclusive para el personal de atención en salud Objetivos: Identificar los microorganismos y su patrón de sensibilidad a antibióticos aislados de celulares y fonendoscopios de estudiantes de medicina de pregrado y postgrado. Metodología: Se tomaron por hisopado 77 muestras entre celulares y fonendoscopios (43/34) y se cultivaron en agar sangre a 37°C por 24-36 horas, se realizaron pruebas específicas para determinar el microorganismo y el patrón de sensibilidad. Resultados: De 43 muestras en celulares se aislaron 92 microorganismos principalmente  Staphylococcus coagulasa negativo (SCN) (51%), Staphylococcus aureus meticilino sensible (SAMS) (14%), Escherichia coli (5%), Klebsiella spp (3%), Acinetobacter spp (2%), Pseudomonas spp (2%), Staphylococcus aureus meticilino resistente (SAMR) (2%). De 34 muestras de fonendosocopios se hallaron 59 microorganismos distribuidos así: SCN (61%), SAMS (15%), SAMR (5%), Pseudomonas spp (3%), Klebsiella spp (3%), E. coli (3%), Acinetobacter spp (2%). El 25% y 13% de los Staphylococcus aureus en fonendos y celulares fueron SAMR, en cuanto a los bacilos Gram negativos en fonendoscopios, la mayoría mostró sensibilidad a los antibióticos empleados a excepción de algunas cepas de Klebsiella spp, Acinetobacter spp y Escherichia coli. En celulares el patrón de sensibilidad fue más heterogéneo, destacando que el 50% de las cepas de Pseudomonas spp fueron resistentes a la Ceftriaxona y el 100% de las cepas de Acinetobacter spp a los β-lactámicos probados. Conclusiones: La colonización de dispositivos médicos y electrónicos por microorganismos patógenos resistentes a diferentes familias de antimicrobianos puede presentar un riesgo de transmisión a pacientes hospitalarios y ambulatorios al exponerlos al contacto con estos.


Author(s):  
T. S. P. J. Jayaweera ◽  
J. L. P. C. Randika ◽  
H. G. C. L. Gamage ◽  
N. N. Udawatta ◽  
W. U. N. T. S. Ellepola ◽  
...  

Aims: Mastitis is one of the very important and most common diseases among dairy cattle globally which leads to severe economical losses in the dairy industry. For the sustainability of the dairy sector it is critical that efficient, economically feasible treatment regime is available for clinical cases of mastitis as a part of the control program with minimum risk for residues in milk. Antimicrobials are the most common drugs of choice for controlling and preventing this devastating condition. But the frequent use of antibiotics leads to the development of resistant bacteria which could have an adverse effect on human health as well. To mitigate this destructive constraint in the industry, identifying the etiology and their susceptibilities to remedial measures are of paramount importance. Hence this study was aimed at isolating and identifying the common bacterial etiology Escherichia coli, Klebsiella spp. and Staphylococcus spp. of mastitis and evaluating the antimicrobial susceptibility of the isolates in order to develop mastitis control strategies in the area. Study Design: Milk samples were collected from mastitic cows in different stages including subclinical and clinical cases based on the results of California Mastitis Test Place and Duration of Study: Samples were collected from dairy farms in Nuwera Eliya District, Sri Lanka and Laboratory investigations were carried out in the Laboratory of Livestock Production, Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanka, Between Aug. 2017 and Nov. 2017. Methodology: E.coli, Klebsiella spp. and Staphylococcus spp. were isolated from 31 milk samples and susceptibility to commonly used antibiotics (Trimethoprim, Oxytetracycline, Chloramphenicol, Cephalexin, Enrofloxacin and Ciprofloxacin) was determined by Kirby Bauer disk diffusion method. Results: The study revealed that the most common isolate was the Klebsiella spp. and it is 54.8% and other two organisms Staphylococcus spp. had 51.6% and Escherichia coli 41.9%. Of all isolated pathogen, 97.1% exhibited resistant to Cephalexin and it was the highest while lowest resistance was to Chloramphenicol (31.4%). Among the other antibiotics, 54.3% of total isolates showed resistance to Trimethoprim followed by 42.9% to Oxytetracycline and Enrofloxacin, 34.3% was resistant to Ciprofloxacin. Resistance to at least one antibiotic was observed for the isolated microorganisms. All the three isolated pathogens are more resistant to Cephalexin. Both E. coli and Klebsiella spp. show 100% resistance to Cephalexin while Staphylococci had 92.9% resistance. This further revealed that E. coli (10%) and Klebsiella spp. (27.3%) are showing the least resistance to Chloramphenicol, None of Staphylococcus spp. (0%) isolated show resistance to Enrofloxacin. Conclusion: Most common organisms isolated were Klebsiella spp. followed by Staphylococcus spp., E. coli and there is a resistance of isolated organisms to some commonly used antibiotics.


2017 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Md Ibrahim Miah ◽  
Tasnia Ahmed ◽  
Saurab Kishore Munshi

Urinary tract infection (UTI) is the infection that occurs in the urinary tract by the invasion of pathogenic bacteria, fungi, virus etc. under some circumstances only. Some common pathogenic bacteria associated with UTI include Escherichia coli, Proteus spp., Streptococcus spp., Staphylococcus spp., Pseudomonas spp. In the ongoing research work, about 30 UTI patients were selected randomly to detect the pathogen responsible for causing urinary tract infection and also to demonstrate their drug resistant traits. Among the 30 samples, about 14 samples were found to harbor Proteus spp. (1×104 cfu/ml to 1×105 cfu/ml). Escherichia coli was found to be the second predominant agent for UTI and recovered from 13 samples. Among the rest 3 samples, Pseudomonas spp. was found in 2 samples and Klebsiella spp. was found in one sample. Identified isolates showed resistance against different ranges of antibiotics. The study findings revealed the fact that emerging drug resistance of different pathogenic bacteria could become the major difficulties in the treatment of infectious diseases like UTI.Stamford Journal of Microbiology, Vol.6(1) 2016: 34-38


2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23


2020 ◽  
Vol 48 (02) ◽  
pp. 78-88
Author(s):  
Maria Brockmann ◽  
Heike Aupperle-Lellbach ◽  
Elisabeth Müller ◽  
Anton Heusinger ◽  
Michael Pees ◽  
...  

Zusammenfassung Gegenstand und Ziel Bakterielle Hautinfektionen kommen bei Reptilien häufig vor. Obwohl viele dieser Infektionen durch multifaktorielle Probleme verursacht werden, ist eine spezifische Behandlung nötig. Ziel der Studie war, das Keimspektrum und die Resistenzlage von aeroben Bakterien in Hautläsionen von Reptilien zu untersuchen. Material und Methoden Tupferproben dermaler Läsionen von 219 Reptilien wurden bakteriologisch untersucht (01/2017–06/2018). Die Identifizierung der Bakterien erfolgte anhand von Selektivnährböden, biochemischen Parametern sowie MALDI-TOF-MS, die Erstellung der Antibiogramme mittels Mikrodilutionsmethode. Ergebnisse Bei den insgesamt identifizierten 306 Keimisolaten handelte es sich überwiegend um gramnegative Spezies. Pseudomonas spp. (n = 48), Citrobacter spp. (n = 31, nur bei Schildkröten), aerobe Sporenbildner (n = 30), Aeromonas spp. (n = 20), Acinetobacter spp. (n = 20), Proteus spp. (n = 15), Staphylococcus spp. (n = 15), Klebsiella spp. (n = 13), Enterococcus spp. (n = 13) sowie Morganella spp. (n = 11) machten den Hauptteil aus, daneben konnten weitere gramnegative (n = 78) und grampositive (n = 12) Bakterienspezies identifiziert werden. Mischkulturen mit 2 (n = 80) oder mehr (n = 16) Keimen traten bei 96 Tieren auf. Von 208 der 306 Isolate wurden Antibiogramme erstellt. Gegenüber Enro- (E) und Marbofloxacin (M) waren viele Isolate sensibel (minimale Hemmkonzentration [MHK] in µg/ml ≤ Grenzwert), beispielsweise Pseudomonas spp. (E: 86,4 % MHK ≤ 0,5; M: 95,5 % MHK ≤ 1), Citrobacter spp. (E: 86,4 % MHK ≤ 0,5; M: 90,9 % MHK ≤ 1) und Aeromonas spp. (E: 75,0 % MHK ≤ 0,5; M: 100 % MHK ≤ 1). Trimethoprim/Sulfamethoxazol erwies sich als wirksam gegen die meisten Citrobacter- (90,9 % MHK ≤ 2/38) und Aeromonas- (75,0 % MHK ≤ 2/38) Isolate. Amikacin war wirksam gegen fast alle Pseudomonas spp. (97,7 % MHK ≤ 16), Citrobacter spp. (95,5 % MHK ≤ 16) sowie Aeromonas spp. (93,8 % MHK ≤ 16). Schlussfolgerung und klinische Relevanz Das Keimspektrum von Reptilienhautläsionen umfasst vor allem gramnegative Bakterien, deren klinische Relevanz für jeden Einzelfall abzuwägen ist. Viele Isolate dieser Studie waren sensibel für Fluorchinolone sowie Aminoglykoside. Da der Einsatz dieser Antibiotika zurückhaltend erfolgen sollte und gegenüber jeder getesteten Antibiotikagruppe auch resistente Isolate identifiziert wurden, wird eine Antibiogrammerstellung empfohlen.


2021 ◽  
Vol 27 (1) ◽  
pp. e2021
Author(s):  
Isabela Fernanda Spinelli Perossi ◽  
Paulo Baptista Martinelli ◽  
Marita Vedovelli Cardoso ◽  
Julieta Rodini Engracia De Moraes

Objective. The aim of this study was to characterize the bacteria of a veterinary autopsy room, their antimicrobial susceptibility profile and testing the efficiency of two sanitizers against these microorganisms. Materials and methods. Three points of the room that professionals do not normally wear personal protective equipment (PPE) getting in direct contact with these bacterias. Anaerobic and aerobic mesophilic count were performed before and after disinfection with hypochlorite and sodium alkylbenzene sulfonate then, isolates were identified by their morphotintorials and biochemical characteristics and their antibiotic susceptibility. Results. Preliminary results indicated that the hypochlorite was the sanitizing agent of choice for surface disinfection and against the most frequent potential pathogenic bacterials isolated such as Staphylococcus spp (75%), E. coli and Klebsiella spp (15%), and Pseudomonas spp (10%). In addition, 25% of the staphylococci were resistant to at least one antimicrobial tested and Klebsiella spp, E. coli, and Pseudomonas spp were taken into consideration, wide antimicrobial resistance tested were observed. Conclusions. The characterization of these bacteria found in the autopsy room is important to alert professionals about the biological risks they are exposed to, as well as the precautions they should take.


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