scholarly journals Physical purity and germination of sugarcane seeds (caryopses) (Saccharum spp.)

2010 ◽  
Vol 32 (2) ◽  
pp. 140-145 ◽  
Author(s):  
Juliana Terezinha Caieiro ◽  
Maristela Panobianco ◽  
João Carlos Bespalhok Filho ◽  
Osvaldo de Castro Ohlson
Keyword(s):  
Plant Breeding ◽  
Seed Viability ◽  
Germination Test ◽  
Paper Substrate ◽  
Breeding Programs ◽  
Saccharum Spp ◽  
Test Study ◽  
Alternating Temperature ◽  
Incubation Temperatures ◽  
Favorable Conditions

Plant breeding is generally done through sexual reproduction even when the species is propagated asexually for commercial exploitation, as for example, in sugarcane. Therefore, the development of procedures to evaluate sugarcane seed viability is important for plant breeding programs. The objective of this research was to develop a methodology for analyzing the viability of sugarcane seeds (Saccharum spp.). Three crosses were used, two biparental crosses and one polycross. For the germination test study, two substrates (paper and sand) and three constant incubation temperatures (25 ºC, 30 ºC and 35 ºC), in the presence of constant light and also an alternating temperatures (20-30 ºC), with 8 hours light (30 ºC) and 16 hours darkness (20 ºC), were studied. Seedlings were evaluated every five days. The results demonstrated that temperature affected sugarcane seed germination with the most favorable conditions being the alternating temperature (20-30 ºC) and the constant temperature of 30 ºC on a paper substrate.

scholarly journals Making waves in Breedbase: An integrated spectral data storage and analysis pipeline for plant breeding programs

tppj ◽  
2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Jenna Hershberger ◽  
Nicolas Morales ◽  
Christiano C. Simoes ◽  
Bryan Ellerbrock ◽  
Guillaume Bauchet ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Spectral Data ◽  
Data Storage ◽  
Analysis Pipeline ◽  
Breeding Programs

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

Integrating Biotechnology into Woody Plant Breeding Programs

1988 ◽  
pp. 433-449 ◽  
Author(s):  
D. E. Riemenschneider ◽  
B. E. Haissig ◽  
E. T. Bingham
Keyword(s):  
Plant Breeding ◽  
Woody Plant ◽  
Breeding Programs

scholarly journals Review: Cost-Effective Unmanned Aerial Vehicle (UAV) Platform for Field Plant Breeding Application

2020 ◽  
Vol 12 (6) ◽  
pp. 998 ◽  
Author(s):  
GyuJin Jang ◽  
Jaeyoung Kim ◽  
Ju-Kyung Yu ◽  
Hak-Jin Kim ◽  
Yoonha Kim ◽  
...  
Keyword(s):  
Plant Breeding ◽  
Unmanned Aerial Vehicle ◽  
High Throughput ◽  
New Technologies ◽  
Cost Effective ◽  
New Wave ◽  
Breeding Programs ◽  
Modern Agriculture ◽  
Aerial Vehicle ◽  
High Throughput Phenotyping

Utilization of remote sensing is a new wave of modern agriculture that accelerates plant breeding and research, and the performance of farming practices and farm management. High-throughput phenotyping is a key advanced agricultural technology and has been rapidly adopted in plant research. However, technology adoption is not easy due to cost limitations in academia. This article reviews various commercial unmanned aerial vehicle (UAV) platforms as a high-throughput phenotyping technology for plant breeding. It compares known commercial UAV platforms that are cost-effective and manageable in field settings and demonstrates a general workflow for high-throughput phenotyping, including data analysis. The authors expect this article to create opportunities for academics to access new technologies and utilize the information for their research and breeding programs in more workable ways.

scholarly journals SSR Marker-Assisted Management of Parental Germplasm in Sugarcane (Saccharum spp. hybrids) Breeding Programs

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 449 ◽  
Author(s):  
Jiantao Wu ◽  
Qinnan Wang ◽  
Jing Xie ◽  
Yong-Bao Pan ◽  
Feng Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Capillary Electrophoresis ◽  
Population Structure ◽  
Ssr Markers ◽  
High Performance ◽  
Breeding Programs ◽  
Saccharum Spp ◽  
Parental Lines ◽  
Assisted Management ◽  
High Performance Capillary Electrophoresis

Sugarcane (Saccharum spp. hybrids) is an important sugar and bioenergy crop with a high aneuploidy, complex genomes and extreme heterozygosity. A good understanding of genetic diversity and population structure among sugarcane parental lines is a prerequisite for sugarcane improvement through breeding. In order to understand genetic characteristics of parental lines used in sugarcane breeding programs in China, 150 of the most popular accessions were analyzed with 21 fluorescence-labeled simple sequence repeats (SSR) markers and high-performance capillary electrophoresis (HPCE). A total of 226 SSR alleles of high-resolution capacity were identified. Among the series obtained from different origins, the YC-series, which contained eight unique alleles, had the highest genetic diversity. Based on the population structure analysis, the principal coordinate analysis (PCoA) and phylogenetic analysis, the 150 accessions were clustered into two distinct sub-populations (Pop1 and Pop2). Pop1 contained the majority of clones introduced to China (including 28/29 CP-series accessions) while accessions native to China clustered in Pop2. The analysis of molecular variance (AMOVA), fixation index (Fst) value and gene flow (Nm) value all indicated the very low genetic differentiation between the two groups. This study illustrated that fluorescence-labeled SSR markers combined with high-performance capillary electrophoresis (HPCE) could be a very useful tool for genotyping of the polyploidy sugarcane. The results provided valuable information for sugarcane breeders to better manage the parental germplasm, choose the best parents to cross, and produce the best progeny to evaluate and select for new cultivar(s).

Dormancy break and germination requirements in acorns of two bottomland Quercus species (Sect. Lobatae) of the eastern United States with references to ecology and phylogeny

2020 ◽  
Vol 30 (3) ◽  
pp. 199-205
Author(s):  
Tracy S. Hawkins
Keyword(s):  
United States ◽  
Cold Stratification ◽  
Eastern United States ◽  
Closely Related Species ◽  
Temperature Regimes ◽  
Quercus Species ◽  
Physiological Dormancy ◽  
Alternating Temperature ◽  
Incubation Temperatures ◽  
Germination Requirements

AbstractQuercus species are ecologically and economically important components of deciduous forests of the eastern United States. However, knowledge pertinent to a thorough understanding of acorn germination dynamics for these species is lacking. The objectives of this research were to determine dormancy break and germination requirements for acorns of two eastern United States bottomland species, Quercus nigra and Quercus phellos (Section Lobatae), and to present results within ecological and phylogenetic contexts. Three replicates of 50 acorns of each species received 0 (control), 6, 12 or 18 weeks of cold stratification, followed by incubation in alternating temperature regimes of 15/6, 20/10, 25/15 and 30/20°C. Eighteen weeks of cold stratification were not sufficient for dormancy break in Q. nigra acorns. Cumulative germination percentages at 4 weeks of incubation were ≥77%, but only in incubation temperatures of 25/15 and 30/20°C. Dormancy break in Q. phellos acorns was achieved with 18 weeks of cold stratification, and cumulative germination percentages were ≥87% at 4 weeks of incubation in all test temperature regimes. Gibberellic acid solutions were not an effective substitute for cold stratification in either species. Phylogenetically, Q. nigra and Q. phellos are closely related species and, ecologically, both grow in the same habitat. Acorns of both species possess deep physiological dormancy (PD), but dormancy break and germination requirements differ in acorns of these two Quercus species.

scholarly journals Seed germination at different stratification temperatures and development of Phytelephas macrocarpa Ruiz & Pavón seedlings

2017 ◽  
Vol 39 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Sidney Alberto do Nascimento Ferreira ◽  
Daniel Felipe de Oliveira Gentil
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seed Viability ◽  
Seedling Development ◽  
Development Stages ◽  
Plastic Bags ◽  
Alternating Temperature

Abstract: Phytelephas macrocarpa (ivory palm) is an Amazonian palm vulnerable to exploitation pressure, as its seeds are widely used in regional handicrafts. The aims of this study were to evaluate the effectiveness of different stratification temperatures in overcoming seed dormancy and to analyze the seedling development stages of this species. In germination under stratification, the seeds were placed in plastic bags containing moistened vermiculite, and maintained at the constant temperatures of 25, 30, 35 and 40 °C, and an alternating temperature from 26 to 40 °C. In the study about the development of seedlings, seeds were sown in vermiculite under plastic cover (growing house), and the evolution of the seedling was evaluated, from the formation of germinative button to the complete blade expansion of the first eophyll. Stratification at alternating temperatures (26 to 40 °C) helped overcoming seed dormancy. Stratification at 25 °C kept the seed viability for nine months. Germination, characterized by the formation of the germinative button took an average of 114 ± 24 days, and the seedling development until the first extended eophyll lasted 244 ± 57 days.

scholarly journals Viability of Carica papaya L. Seeds by the Tetrazolium Test

2018 ◽  
Vol 10 (2) ◽  
pp. 335 ◽  
Author(s):  
Sara M. C. Carvalho ◽  
Salvador B. Torres ◽  
Erivanessa C. Sousa ◽  
Danielle M. M. Sousa ◽  
Kleane T. O. Pereira ◽  
...  
Keyword(s):  
Decision Making ◽  
Carica Papaya ◽  
Seed Viability ◽  
Germination Test ◽  
Long Period ◽  
Physiological Quality ◽  
Rapid Tests ◽  
Papaya Seeds ◽  
Tetrazolium Test

Germination test for papaya seeds demands thirty days to obtain the results, which is a long period for decision-making. Thus, rapid tests become essential tools to evaluate seed viability, and the tetrazolium test is an alternative for this purpose. Hence, this study aimed to establish the adequate procedure to apply this test, in order to reduce the time of evaluation of papaya seeds. For that, four tetrazolium solution concentrations (0.05, 0.1, 0.5 and 1%) and three staining times (3, 6 and 9 hours) were tested at temperatures of 35 and 40 ºC. The experimental design was completely randomized in 4 × 3 + 1 factorial scheme (concentrations × staining times + one control = germination test). It was found that the tetrazolium test allows to evaluate the physiological quality of papaya seeds, thus reducing the time for decision-making. For the test, we recommend using the concentrations of 0.1% for 9 hours or 1% for 6 hours of staining, at temperature of 40 ºC.

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