scholarly journals Use of X-ray to evaluate damage caused by weevils in cowpea seeds

2010 ◽  
Vol 28 (4) ◽  
pp. 472-476 ◽  
Author(s):  
Roberto de A Melo ◽  
Victor Augusto Forti ◽  
Silvio M Cicero ◽  
Ana DLC Novembre ◽  
Paulo César T de Melo

In Brazil, the cowpea [Vigna unguiculata (L.) Walp], is important in the Northeast Region, where it is typically grown on family farms. The importance of the damage caused to the seed quality of this species by stored pests has been described in various studies. Using X-ray, it is possible to see the internal seed structures and identify possible changes and damage. The objective of this study was to identify the damage caused by the weevil [Callosobruchus maculatus (Fabr.)] by analyzing X-ray and evaluate its relationship to the physiological quality of the cowpea seed. Three cultivars were used (IPA-206, BRS-Pajeu and BRS-Potengi) and two lines (L 281.005 and L ESP 10). The samples were exposed to X-ray and germination test to determine the cause-effect relationship between weevil damage and seed germination. X-ray images were evaluated to determine damage severity and location in the seed. Seed damage classified as severe, located in the embryonic axis or in the cotyledons, resulted in abnormal seedlings or dead seeds. The X-ray test, therefore, is efficient for evaluating weevil damage in cowpea seeds and the damage caused to be associated with any resulting adverse germination effects.

2014 ◽  
Vol 36 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Elizabeth Rosemeire Marques ◽  
Eduardo Fontes Araújo ◽  
Roberto Fontes Araújo ◽  
Sebastião Martins Filho ◽  
Plínio César Soares

The objective of this study was to evaluate dormancy and physiological quality of seeds of rice cultivars during storage in different environments. After harvesting, the seeds of three rice cultivars (Seleta, Curinga and Relâmpago) were dried in the sun, to reach moisture content at around 13%. Then, they were packed in paper and stored in four environments: 5 ± 2 ºC / 70 ± 5% RH, 12 ± 2 ºC / 70 ± 5% RH, 18 ± 2 ºC / 65 ± 5% RH and in uncontrolled condition of temperature and relative humidity (natural). Physiological quality was evaluated at the beginning and at 3, 6, 9 and 12 months of storage by germination test, electrical conductivity, accelerated aging and sand emergence. The experiment was conducted in split plots in a completely randomized design with three replications. Environmental factor was applied in the plots, cultivars in the subplots and storage period in subplots. Cultivar Seleta showed higher dormancy, which was surpassed during storage regardless of environment conservation. In general, seeds stored in natural environment showed lower physiological quality. Only the seeds of cultivar Seleta, regardless of the environment, maintained germination above the minimum required for commercialization until six months of storage.


2017 ◽  
Vol 39 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Cristian Rafael Brzezinski ◽  
Julia Abati ◽  
Fernando Augusto Henning ◽  
Ademir Assis Henning ◽  
José de Barros França Neto ◽  
...  

Abstract: The aim of this work was to evaluate the effect of different spray volumes, via industrial treatment, on the physiological quality of soybean seeds with different levels of vigor. The experimental design was completely randomized in a 2x5 factor scheme, with four replications. Factors consisted in two levels of seed vigor (high and low) and five spray volumes (0, 600, 1200, 1800 and 2400 mL. 100 kg-1). Products used to obtain the volumes were: fungicide (carbendazin + thiram); insecticide (imidacloprid + thiodicarb); nematicide (abamectin); micronutrients (cobalt, molybdenum and zinc); polymer (peridiam); biostimulants (kinetin + gibberellic acid) and inoculum (Bradyrhizobium japonicum). The used cultivars were BRS 360 RR and BRS 1010 IPRO. The physiological seed quality was determined by the following evaluations: germination, first count of the germination test, seedling emergence in sand, emergency speed index, total length of shoot and root of the seedlings. In the different spray volumes, high vigor soybean seeds have higher physiological quality than low vigor seeds. The increased spray volumes in seed treatments reduce the physiological quality of low vigor soybean seeds.


2021 ◽  
Vol 43 ◽  
Author(s):  
Alessandra da Silva Ribeiro ◽  
Tássia Fernanda Santos Neri ◽  
André Dantas de Medeiros ◽  
Carla do Carmo Milagres ◽  
Laércio Junio da Silva

Abstract: Technologies based on electromagnetic radiation, such as the X-ray technique, has contributed to the establishment of new and promising methodologies for evaluating seed quality. This study aimed to relate parameters based on semi-automated analysis of radiographs of crambe seeds to their physiological quality. Radiographic images of seeds from 10 seed lots of cultivar FMS Brilhante were semi-automatically analyzed using ImageJ® software. Measurements of morphometric characteristics and tissue integrity were obtained for the seeds, as well as individually for the seed embryo. Following X-ray test, the seeds were subject to germination and seedling growth test. It was possible to visualize the internal structures of the seeds in the radiographs. There were differences in the physical parameters obtained by the semi-automated analysis of the radiographs between the seed lots. Also, the lots differed regarding the physiological quality of the seeds. Morphometric characteristics and tissue integrity, especially for the seed embryo, showed high correlation with the seed physiological quality. Therefore, this work presents an efficient approach to rapid and non-destructively assess the quality of crambe seeds.


2021 ◽  
Vol 17 (1) ◽  
pp. 25-35
Author(s):  
Mariana Zampar Toledo ◽  
Daniel Junior Bilck ◽  
Cleiton Banhara Machado

Reduced quality ofcowpea due to late harvest and therefore pathogen incidence may influence the results of the germination test, whenever losses are associated with the agentsadhered to seed coat;in that context, chemical treatmentof seeds during the evaluations may reflect more accurately physiological quality of the samples, which in turn may assurethe emergence results of healthy seedlings and establishment of appropriate plant populations inthe field. This study had the objective ofevaluating the effects of fungicide treatment on minimizing physiological quality losses of cowpea seedsdue to lateharvestin germination and seedling development tests. The experimental design was the completely randomized with four replications. Treatments consisted of cowpea, cultivar BRS Guariba, harvested at different times (0, 3, 6, 9, 12 and 15 days after physiological maturity)andtreated or not with fungicide carboxim+thiramfor seed quality tests. Seeds were initially characterized by seed moisture and mass. Afterwards, sampleswere evaluated by tests ofgermination percentage, germination first count, seedling length and dry matter. Data was submitted to variance analysisand means were analyzed as a 2x6 factorial. Means from chemical treatment and harvest times were compared by the Tukey test and regression analysis (p≤0.05), respectively, with subsequent unfolding of any significant interactions. Delayed harvest of cowpea seeds sharply reduces germination and vigor; fungicide treatment before performing physiological tests enables to establish thatseed quality losses can bein part caused by pathogen vehiculationonthe seed coat.


2018 ◽  
Vol 48 (4) ◽  
pp. 461-467
Author(s):  
Rogério Luciano Severiano ◽  
Paloma Rayane Pinheiro ◽  
Francisco Guilhien Gomes Júnior ◽  
Andréa Celina Ferreira Demartelaere ◽  
Márcio Dias Pereira

ABSTRACT The germination of papaya seeds is slow and uneven, usually due to inhibitors present in the sarcotesta; however, some removal procedures may cause physical damage, negatively interfering in the physiological quality and field performance. This study aimed to evaluate the physical and physiological quality of papaya seeds submitted to methods of sarcotesta removal by image analysis. Papaya Formosa ‘Tainung 01' seeds were submitted to the following methods of sarcotesta removal: fermentation + sieve, sand + sieve, sieve and blender + sieve. After the sarcotesta removal, the seeds were submitted to tests of germination, first germination count, germination speed and seedling length. For the image analysis, the seeds without sarcotesta were submitted to the X-ray test and to a computerized analysis of seedling images by the SVIS® software. The methods used were only promising for the sarcotesta removal, while the blender + sieve method obtained the highest physical and physiological seed quality, in addition to performing the best sarcotesta removal. However, the image analysis using the SVIS® software allowed to identify the high physical and physiological seed quality using the blender + sieve method, which promoted the highest efficiency in removing the sarcotesta.


Author(s):  
Hellismar W. da Silva ◽  
Luís S. R. Vale Junior ◽  
Camila F. Silva ◽  
Rannie do C. Souza ◽  
Renato S. Soares

ABSTRACT Drying air temperature has direct influence on seed quality, especially for vegetable species harvested with high moisture content. This study aimed to determine and model the drying curves of ‘Cabacinha’ pepper seeds (Capsicum chinense L.) at different temperatures, and to evaluate their physiological quality after drying and during storage. The experimental design was completely randomized in a 3 x 3 factorial scheme, with three drying temperatures (35, 38 and 42 °C) and three storage periods (0, 4 and 8 months), with four replicates. Seed quality was evaluated by germination test, first germination count, accelerated aging, electrical conductivity, seedling emergence, and speed of emergence. The Two Terms is the model that best represents the drying curves of ‘Cabacinha’ pepper seeds at temperatures of 35, 38 and 42 °C. Higher germination and vigor values are obtained in seeds dried at 35 and 38 °C. Drying at 42 °C and storage reduce the physiological quality of the seeds.


2019 ◽  
Vol 37 ◽  
Author(s):  
M.O. ASSIS ◽  
E.F. ARAUJO ◽  
F.C.L. FREITAS ◽  
L.J. SILVA ◽  
R.F. ARAUJO

ABSTRACT: Pre-harvest desiccation is an important strategy to anticipate harvesting, preventing the seed from being left in the field in unfavorable climatic conditions. However, little is known about the use of desiccants and their application times on cowpea (Vigna unguiculata). The objective of this study was to evaluate the effect of desiccant application at different seasons and of desiccant herbicides on productivity and physiological quality of cowpea seeds. Five dessicants were evaluated: paraquat, paraquat sequential application, glufosinate- ammonium, glyphosate with sequential application of paraquat and paraquat + diuron mixture applied in four seasons: 50% of the pods with green coloration and with seeds formed (season I); 70% of the pods in the purple coloration (season II), 90% of the pods in the coloration brown (season III) and 100% of the pods in the coloration brown (season IV), plus a control without the application of desiccants, in which harvesting was performed when 100% of the pods were brown. The number of days of anticipation of harvest, productivity, uniformity test, mass of one hundred seeds, germination and vigor of the seeds were evaluated. None of desiccation strategies affect the physiological seed quality, independent of the application season. The desquaments paraquat, glufosinate-ammonium and the paraquat+diuron mixture, when applied in seasons I and II, provided anticipation of the harvest in up to 13 days and 9 days, respectively, however, the size of the seeds and consequently the productivity were affected.


2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Mary W. Njonjo ◽  
James W. Muthomi ◽  
Agnes W. Mwang’ombe

Over 80% of farmers in the developing countries use seeds from the informal seed supply that is of unknown quality status with low physical purity, reduced vigour, and contamination with seed-borne pathogens. A survey involving 114 farmers was conducted in Makueni and Taita counties using a semistructured questionnaire to determine cowpea production practices. Forty-seven cowpea seed samples were collected from farmers, and thirty-four were collected from markets and analysed for physical and physiological quality. The data from the questionnaire were analysed using SPSS package. Majority, over 76% of farmers used farm-saved seeds and intercropped cowpea with cereals (56%). The common storage container was polythene bags (56%), and farmers did not treat the seeds. The seed was below the recommended purity standard of 98%. In Taita, farm-saved and market-sourced seeds met the recommended 75% germination at 82.7% and 76.8%, respectively. Even though the germination standard was met, seeds were of low physical purity and reduced vigour. Farmers should be enlightened on recommended production practices, methods of harvesting, and postharvest handling practices to reduce seed quality loss during storage and maximize production.


2020 ◽  
Vol 33 (4) ◽  
pp. 878-887
Author(s):  
ELIZETH REGINA RAISSE ◽  
MIQUÉIAS DE OLIVEIRA ASSIS ◽  
EDUARDO FONTES ARAUJO ◽  
FRANCISCO CLÁUDIO LOPES DE FREITAS ◽  
ROBERTO FONTES ARAUJO

ABSTRACT Information about the effect of herbicides on cowpea seed quality is scarce. The objective of this work was to evaluate the effect of using chemical desiccants at pre-harvest on the anticipation of harvest and physiological quality of cowpea seeds of the cultivar BRS-Tumucumaque, before and after storage. Two experiments were conducted: experiment 1 was conducted under a randomized block design, with four replications; the treatments consisted of seven herbicides (carfentrazone-ethyl, saflufenacil, glyphosate, paraquat, flumioxazin, glufosinate-ammonium, and diquat) and a control treatment; anticipation of harvest, and seed water content, germination, and vigor were evaluated. The seeds were stored for six months in controlled environment and not-controlled environment rooms and evaluated for water content, germination, and vigor in Experiment 2, which was conducted in a 7^2+1 factorial arrangement, with four replications; the first factor consisted of the seven herbicides, the second factor was the two storage environments, plus a control treatment. The results showed 3 to 9 days of anticipation of harvest relative to the control. The glufosinate -ammonium herbicide negatively affected seed germination and vigor. The glyphosate herbicide decreased seed germination and vigor after storage. The physiological quality of cowpea seeds at the post -harvest period and after six months of storage was not affected by the carfentrazone-ethyl and flumioxazin herbicides. Paraquat and diquat were the most efficient herbicides for the anticipation of harvest (nine days). Glyphosate and glufosinate -ammonium were the most harmful herbicides to seed physiological quality. The controlled environment room was the most adequate environment for seed conservation.


Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 286-292
Author(s):  
Cristiane Fortes Gris ◽  
Edila Vilela de Resende Von Pinho ◽  
Maria Laene de Moreira Carvalho ◽  
Rafael Parreira Diniz ◽  
Thaís de Andrade

Differences in levels of lignin in the plant between conventional and transgenic cultivars RR has been reported by several authors, however, there are few studies evaluating the influence of spraying of glyphosate on the lignin in the plant and RR soybean seeds. The aim of this study was to evaluate the physiological quality of RR transgenic soybean seeds and the lignin contents of plants sprayed with the herbicide glyphosate. The assays were conducted both in greenhouse and field in the municipality of Lavras, MG, in the agricultural year 2007/08. The experiment was arranged in a splitplot design with four replicates, considering the treatments hand weeding and herbicide glyphosate as plots, and five RR soybean cultivars (BRS 245 RR, BRS 247 RR, Valiosa RR, Silvânia RR and Baliza RR) as splitplots. In the greenhouse, the cultivars tested were BRS 245 RR and Valiosa RR in a randomized block design with four replicates. The sprayings were carried out at stages V3, V7 and early R5 (3L/ha). The 1000 seed weight, mechanical injury, germination and germination velocity index, emergence velocity index, accelerated aging, electrical conductivity and water soaking seed test, lignin content in the seed coat, in the stem and legumes were determined. The spraying of glyphosate herbicide, in greenhouse and field, did not alter the physiological quality of seeds and the lignin contents in the plant.


Sign in / Sign up

Export Citation Format

Share Document