scholarly journals Peracute bovine mastitis caused by Klebsiella pneumoniae

2008 ◽  
Vol 60 (2) ◽  
pp. 485-488 ◽  
Author(s):  
M.G. Ribeiro ◽  
R.G. Motta ◽  
A.C. Paes ◽  
S.D. Allendorf ◽  
T. Salerno ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Bovine Mastitis

scholarly journals Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis

2021 ◽  
Author(s):  
Jesús Silva-Sanchez ◽  
Humberto Barrios-Camacho ◽  
Emmanuel Hernández-Rodriguez ◽  
Josefina Duran-Bedolla ◽  
Alejandro Sanchez-Perez ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Bovine Mastitis

scholarly journals Whole-Genome Sequencing of Gram-Negative Bacteria Isolated From Bovine Mastitis and Raw Milk: The First Emergence of Colistin mcr-10 and Fosfomycin fosA5 Resistance Genes in Klebsiella pneumoniae in Middle East

2021 ◽  
Vol 12 ◽  
Author(s):  
Yasmine H. Tartor ◽  
Norhan K. Abd El-Aziz ◽  
Rasha M. A. Gharieb ◽  
Hend M. El Damaty ◽  
Shymaa Enany ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Genome Sequencing ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Raw Milk ◽  
Whole Genome ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli

Antimicrobial resistance is a major concern in the dairy industry. This study investigated the prevalence, antimicrobial resistance phenotypes, and genome sequencing of Gram-negative bacteria isolated from clinical (n = 350) and subclinical (n = 95) bovine mastitis, and raw unpasteurized milk (n = 125). Klebsiella pneumoniae, Aeromonas hydrophila, Enterobacter cloacae (100% each), Escherichia coli (87.78%), and Proteus mirabilis (69.7%) were the most prevalent multidrug-resistant (MDR) species. Extensive drug-resistance (XDR) phenotype was found in P. mirabilis (30.30%) and E. coli (3.33%) isolates. Ten isolates (four E. coli, three Klebsiella species and three P. mirabilis) that displayed the highest multiple antibiotic resistance (MAR) indices (0.54–0.83), were exposed to whole-genome sequencing (WGS). Two multilocus sequence types (MLST): ST2165 and ST7624 were identified among the sequenced E. coli isolates. Three E. coli isolates (two from clinical mastitis and one from raw milk) belonging to ST2165 showed similar profile of plasmid replicon types: IncFIA, IncFIB, IncFII, and IncQ1 with an exception to an isolate that contained IncR, whereas E. coli ST7624 showed a different plasmid profile including IncHI2, IncHI2A, IncI1α, and IncFII replicon types. ResFinder findings revealed the presence of plasmid-mediated colistin mcr-10 and fosfomycin fosA5 resistance genes in a K. pneumoniae (K1) isolate from bovine milk. Sequence analysis of the reconstructed mcr-10 plasmid from WGS of K1 isolate, showed that mcr-10 gene was bracketed by xerC and insertion sequence IS26 on an IncFIB plasmid. Phylogenetic analysis revealed that K1 isolate existed in a clade including mcr-10-harboring isolates from human and environment with different STs and countries [United Kingdom (ST788), Australia (ST323), Malawi (ST2144), Myanmar (ST705), and Laos (ST2355)]. This study reports the first emergence of K. pneumoniae co-harboring mcr-10 and fosA5 genes from bovine milk in the Middle East, which constitutes a public health threat and heralds the penetration of the last-resort antibiotics. Hence, prudent use of antibiotics in both humans and animals and antimicrobial surveillance plans are urgently required.

scholarly journals Klebsiella pneumoniae isolated from bovine mastitis is cytopathogenic for bovine mammary epithelial cells

2020 ◽  
Vol 103 (4) ◽  
pp. 3493-3504
Author(s):  
Jia Cheng ◽  
Jv Zhang ◽  
Bo Han ◽  
Herman W. Barkema ◽  
Eduardo R. Cobo ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Klebsiella Pneumoniae ◽  
Mammary Epithelial Cells ◽  
Bovine Mastitis ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells

scholarly journals Detection and Molecular Characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-Lactamases from Bovine Mastitis Isolates in the United Kingdom

2013 ◽  
Vol 58 (2) ◽  
pp. 789-794 ◽  
Author(s):  
Dorina Timofte ◽  
Iuliana E. Maciuca ◽  
Nicholas J. Evans ◽  
Helen Williams ◽  
Andrew Wattret ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
United Kingdom ◽  
Klebsiella Pneumoniae ◽  
Bovine Mastitis ◽  
Conjugative Plasmid ◽  
Sensitivity Testing ◽  
Content Type ◽  
E Coli ◽  
The United Kingdom

ABSTRACTRecent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in twoEscherichia coliand threeKlebsiella pneumoniaesubsp.pneumoniaeisolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids.E. coliisolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carriedblaCTX-M-15andblaTEM-1, whileK. pneumoniaesubsp.pneumoniaeisolates carriedblaSHV-12andblaTEM-1. Conjugation experiments demonstrated thatblaCTX-M-15andblaTEM-1were carried on a conjugative plasmid inE. coli, and PBRT identified this to be an IncI1 plasmid. The resistance genes were nontransferable inK. pneumoniaesubsp.pneumoniaeisolates. Moreover, in theE. coliisolates, an association of ISEcp1 and IS26withblaCTX-M-15was found where the IS26element was inserted upstream of both ISEcp1and theblaCTX-Mpromoter, a genetic arrangement highly similar to that described in some United Kingdom human isolates. We report the first cases in Europe of bovine mastitis due toE. coliCTX-M-15 and also of bovine mastitis due toK. pneumoniaesubsp.pneumoniaeSHV-12 β-lactamases in the United Kingdom. We also describe the genetic environment ofblaCTX-M-15and highlight the role that IncI1 plasmids may play in the spread and dissemination of ESBL genes, which have been described in both human and cattle isolates.

scholarly journals First evidence of Klebsiella pneumoniae infection in Aceh cattle: Pathomorphology and antigenic distribution in the lungs

2021 ◽  
Vol 14 (4) ◽  
pp. 1007-1013
Author(s):  
Darniati Darniati ◽  
Surachmi Setiyaningsih ◽  
Dewi Ratih Agungpriyono ◽  
Ekowati Handharyani
Keyword(s):  
Klebsiella Pneumoniae ◽  
Chronic Infection ◽  
Interstitial Fibrosis ◽  
Acute Infection ◽  
Bovine Mastitis ◽  
Foodborne Pathogen ◽  
Wall Thickening ◽  
Alveolar Wall ◽  
Chronic Infections ◽  
Bronchial Dilatation

Background and Aim: Klebsiella pneumoniae is an emerging zoonotic and foodborne pathogen worldwide. Hypervirulent K. pneumoniae (hvKp) was reported as the causative agent of bovine mastitis. This is the first study in Indonesia that has been conducted to determine the capsular serotype of K. pneumoniae, pulmonary gross pathology and histopathology, and distribution of hvKp in the lungs of Aceh cattle. Materials and Methods: The presence of K. pneumoniae in Aceh cattle was investigated in two slaughterhouses in Banda Aceh and Aceh Besar, Indonesia. Lung tissues with gross pathological lesions were collected from 15 cattle presenting with depression, dehydration, or cachexia. The confirmation and capsular serotyping of K. pneumoniae isolates were performed using polymerase chain reaction. The tissues were stained with hematoxylin-eosin and immunohistochemistry to observe the histopathological lesions and the distribution of the hvKp antigens. Results: The pneumonic lesions identified in the lungs of Aceh cattle included hyperemia, hemorrhage, consolidation, and atelectasis. K. pneumoniae was isolated in all 15 lung tissues with pathological pneumonic lesions. Two patterns of infection were observed histopathologically. Acute infection was characterized by hyperemia, inflammatory cell infiltration, hemorrhage, bronchiolar epithelium hyperplasia, bronchial and bronchiolar obstruction with purulent exudates, edema, and atelectasis. On the other hand, chronic infection was defined by macrophage infiltration, emphysema, bronchial dilatation, pleural fibrosis, and alveolar wall thickening by interstitial fibrosis. Immunohistochemical staining using monospecific antisera induced by the hvKp isolate confirmed the presence of K. pneumoniae-specific antigens in the acute infection, predominantly in the bronchiolar, vascular, and alveolar areas. In contrast, generally diffuse infiltrates were found in the pleura and interstitial alveolar areas in chronic infection. Conclusion: hvKp can be detected in the lungs of Aceh cattle, representing acute and chronic infections. The distribution of Klebsiella antigens in the lung tissue was consistent with the histopathological findings.

scholarly journals CTX-M1 ESBL-Producing Klebsiella pneumoniae subsp. pneumoniae Isolated from Cases of Bovine Mastitis

2010 ◽  
Vol 48 (10) ◽  
pp. 3822-3823 ◽  
Author(s):  
C. Locatelli ◽  
L. Scaccabarozzi ◽  
G. Pisoni ◽  
P. Moroni
Keyword(s):  
Klebsiella Pneumoniae ◽  
Bovine Mastitis

scholarly journals Genetic Characterization of CTX-M-2-Producing Klebsiella pneumoniae and Klebsiella oxytoca Associated With Bovine Mastitis in Japan

2021 ◽  
Vol 8 ◽  
Author(s):  
Takeshi Tsuka ◽  
Hiroichi Ozaki ◽  
Daisuke Saito ◽  
Toshiyuki Murase ◽  
Yoshiharu Okamoto ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Klebsiella Oxytoca ◽  
Bovine Mastitis ◽  
Animal Husbandry ◽  
Polymorphism Analysis ◽  
Total Prevalence ◽  
Digestion Pattern ◽  
Causative Agents ◽  
In The Wild ◽  
Mastitic Milk

CTX-M-2-producing Klebsiella oxytoca (K. oxytoca) has not received much attention in animal husbandry compared with Klebsiella pneumoniae (K. pneumoniae), a major reservoir of extended-spectrum β-lactamase (ESBL) genes. Bacteriological examinations of 1,466 mastitic milk samples between October 2012 and December 2014 were conducted. Ninety-five K. pneumoniae isolates (total prevalence: 6.5%) and 81 K. oxytoca isolates (total prevalence: 5.5%) were obtained. Seventeen K. pneumoniae isolates obtained from 15 animals reared on 11 farms and 9 K. oxytoca isolates obtained from 9 animals reared on the same farm were phenotypically confirmed to be ESBL producers. All nine ESBL-producing K. oxytoca isolates were obtained from one farm between June and November 2013 and related to a significantly (p < 0.05) higher monthly prevalence of mild mastitis (in June, August, September, October, and November 2013). Pulsed-field gel electrophoresis (PFGE) patterns of ESBL-producing K. pneumoniae isolates were distinguished from each other by more than 6-band differences except for two isolates from two animals, whereas all nine K. oxytoca isolates showed an identical PFGE pattern. Transferability of the blaCTX−M−2 gene was found in 14 K. pneumoniae and 9 K. oxytoca isolates by conjugation analysis. Of these isolates, the blaCTX−M−2 gene was detected on plasmids belonging to the incompatibility (Inc) groups P and N derived from five K. pneumoniae and nine K. oxytoca isolates, respectively, although the plasmids from the remaining nine K. pneumoniae were untypeable. All the transconjugants exhibited elevated minimum inhibitory concentrations of ampicillin, cefotaxime, and ceftiofur compared with those in the wild-type, recipient strain. Restriction fragment length polymorphism analysis demonstrated that the IncN plasmids extracted from eight of nine transconjugants, which received resistance against β-lactams from K. oxytoca, showed an identical DraI digestion pattern. These results suggest that the CTX-M-2-producing K. oxytoca strain with the above-mentioned characteristics may have clonally spread within a farm, whereas the blaCTX−M−2 gene in K. pneumoniae possibly disseminated among the farms through different plasmids. Thus, monitoring of ESBL genes, including the blaCTX−M−2 gene, among causative agents of bacterial mastitis in cows can help to develop relevant treatments and control practices.

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