Genetic Characterization of CTX-M-2-Producing Klebsiella pneumoniae and Klebsiella oxytoca Associated With Bovine Mastitis in Japan

CTX-M-2-producing Klebsiella oxytoca (K. oxytoca) has not received much attention in animal husbandry compared with Klebsiella pneumoniae (K. pneumoniae), a major reservoir of extended-spectrum β-lactamase (ESBL) genes. Bacteriological examinations of 1,466 mastitic milk samples between October 2012 and December 2014 were conducted. Ninety-five K. pneumoniae isolates (total prevalence: 6.5%) and 81 K. oxytoca isolates (total prevalence: 5.5%) were obtained. Seventeen K. pneumoniae isolates obtained from 15 animals reared on 11 farms and 9 K. oxytoca isolates obtained from 9 animals reared on the same farm were phenotypically confirmed to be ESBL producers. All nine ESBL-producing K. oxytoca isolates were obtained from one farm between June and November 2013 and related to a significantly (p < 0.05) higher monthly prevalence of mild mastitis (in June, August, September, October, and November 2013). Pulsed-field gel electrophoresis (PFGE) patterns of ESBL-producing K. pneumoniae isolates were distinguished from each other by more than 6-band differences except for two isolates from two animals, whereas all nine K. oxytoca isolates showed an identical PFGE pattern. Transferability of the blaCTX−M−2 gene was found in 14 K. pneumoniae and 9 K. oxytoca isolates by conjugation analysis. Of these isolates, the blaCTX−M−2 gene was detected on plasmids belonging to the incompatibility (Inc) groups P and N derived from five K. pneumoniae and nine K. oxytoca isolates, respectively, although the plasmids from the remaining nine K. pneumoniae were untypeable. All the transconjugants exhibited elevated minimum inhibitory concentrations of ampicillin, cefotaxime, and ceftiofur compared with those in the wild-type, recipient strain. Restriction fragment length polymorphism analysis demonstrated that the IncN plasmids extracted from eight of nine transconjugants, which received resistance against β-lactams from K. oxytoca, showed an identical DraI digestion pattern. These results suggest that the CTX-M-2-producing K. oxytoca strain with the above-mentioned characteristics may have clonally spread within a farm, whereas the blaCTX−M−2 gene in K. pneumoniae possibly disseminated among the farms through different plasmids. Thus, monitoring of ESBL genes, including the blaCTX−M−2 gene, among causative agents of bacterial mastitis in cows can help to develop relevant treatments and control practices.

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Mutations Which Uncouple Transport and Phosphorylation in the d-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14

Journal of Bacteriology ◽

10.1128/jb.185.7.2267-2276.2003 ◽

2003 ◽

Vol 185 (7) ◽

pp. 2267-2276 ◽

Cited By ~ 7

Author(s):

Susanne Otte ◽

Annette Scholle ◽

Sevket Turgut ◽

Joseph W. Lengeler

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Klebsiella Oxytoca ◽

Single Copy ◽

High Rate ◽

Phosphotransferase System ◽

Good Efficiency ◽

In The Wild ◽

Different Strains ◽

K 12

ABSTRACT Mutants of Escherichia coli K-12 were isolated which lack the normal phosphotransferase system-dependent catabolic pathway for d-mannitol (Mtl). In some mutants the pts genes for the general proteins enzyme I and histidine protein of the phosphoenolpyruvate-dependent carbohydrate phosphotransferase systems were deleted. Other mutants expressed truncated mannitol-specific enzymes II (IIMtl) which lacked the IIAMtl or IIBAMtl domain(s), and the mtlA genes originated either from E. coli K-12 or from Klebsiella pneumoniae 1033-5P14. The dalD gene from Klebsiella oxytoca M5a1 was cloned on single-copy plasmids and transformed into the strains described above. This gene encodes an NAD-dependent d-arabinitol dehydrogenase (DalD) which converts d-arabinitol into d-xylulose and also converts d-mannitol into d-fructose. The different strains were used to isolate mutations which allow efficient transport of mannitol through the nonphosphorylated IIMtl complexes by selecting for growth on this polyhydric alcohol. More than 40 different mutants were analyzed to determine their ability to grow on mannitol, as well as their ability to bind and transport free mannitol and, after restoration of the missing domain(s), their ability to phosphorylate mannitol. Four mutations were identified (E218A, E218V, H256P, and H256Y); all of these mutations are located in the highly conserved loop 5 of the IIC membrane-bound transporter, and two are located in its GIHE motif. These mutations were found to affect the various functions in different ways. Interestingly, in the presence of all IIMtl variants, whether they were in the truncated form or in the complete form, in the phosphorylated form or in the nonphosphorylated form, and in the wild-type form or in the mutated form, growth occurred on the low-affinity analogue d-arabinitol with good efficiency, while only the uncoupled mutated forms transported mannitol at a high rate.

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Антимікробна резистентність провідних збудників інфекцій сечових шляхів у Києві (Україна)

International Journal of Antibiotics and Probiotics ◽

10.31405/ijap.1-2.17.03 ◽

2017 ◽

Vol 1 (2) ◽

pp. 48-60

Author(s):

A.G. Salmanov ◽

A.V. Rudenko

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Staphylococcus Epidermidis ◽

Klebsiella Oxytoca ◽

Enterobacter Aerogenes ◽

Enterobacter Cloacae ◽

Proteus Vulgaris ◽

Providencia Rettgeri ◽

E Coli

Мета роботи — вивчити резистентність до антибіотиків бактеріальних збудників інфекцій сечових шляхів (ІСШ), виділених у пацієнтів урологічного стаціонару в м. Києві. Матеріали і методи. Досліджено 1612 штамів бактерій, виділених із сечі хворих з ІСШ (цистит, уретрит, пієлонефрит), госпіталізованих в урологічне відділення ДУ «Інститут урології НАМН України» у м. Києві протягом 2016 р. Серед пацієнтів переважали жінки — 1201 (74,5 %). Вік хворих становив від 17 до 74 років. Для збору даних використано медичну документацію лікарні. Мікробіологічні дослідження виконано у лабораторії мікробіології ДУ «Інститут урології НАМН України». Аналізували результати культурального дослідження зразків сечі, зібраних за наявності клінічних ознак ІСШ. Дослідження клінічного матеріалу та інтерпретацію отриманих результатів проводили загальноприйнятими методами. Вивчено чутливість уропатогенів до 31 антибіотика дискодифузійним методом відповідно до рекомендацій Інституту клінічних та лабораторних стандартів США (Clinical and Laboratory Standards Institute (CLSI)). Результати та обговорення. Аналіз мікробного спектра сечі виявив домінування серед уропатогенів штамів Escherichia coli (32,0 %), Enterococcus faecalis (19,5 %), Klebsiella pneumoniae (10,9 %), Staphylococcus epidermidis (8,9 %), S. haemolyticus (6,5 %) та Pseudomonas aeruginosa (6,4 %). Частка Enterococcus faecium, Enterobacter aerogenes і Streptococcus viridans становила відповідно 2,5, 2,2 і 1,6 %, Enterobacter cloacae, Klebsiella oxytoca, Acinetobacter baumannii, Proteus vulgaris та Providencia rettgeri — менше 1,0 %. У більшості випадків (69,7 %) мікроорганізми виділено у монокультурі, у решті випадків — у мікробних асоціа- ціях. Високу резистентність до тестованих антибіотиків виявили штами E. aerogenes (45,1 %), E. cloacae (45,7 %), E. faecium (40,9 %), E. faecalis (40,7 %), E. coli (39,9 %), P. aeruginosa (34,0 %), K. pneumoniae (28,6 %). Найбільш активними до уропатогенів були іміпенем (E. coli — 87,6 %, P. aeruginosa — 75,7 %, E. cloacae — 67,3 %, E. aerogenes — 72,6 %, K. pneumoniae — 93,2 %), меропенем (E. coli — 89,1 %, P. aeruginosa — 76,7 %, K. pneumoniae — 82,6 %), лефлоцин (E. coli — 74,5 %, ентерококи — 78,7 %, P. aeruginosa — 76,7 %, E. cloacae — 73,9 %, E. aerogenes — 80,4 %, K. pneumoniae — 83,5 %), амоксицилін/клавуланат (ентерококи — 84,6 %), фурагін (ентерококи — 82,6 %), цефоперазон (K. pneumoniae — 89,2 %, P. aeruginosa — 73,8 %), цефтріаксон (K. pneumoniae — 80,1 %). Висновки. Антибіотикорезистентність збудників ІСШ — важлива терапевтична проблема. Найбільшою активністю до уропатогенів характеризуються іміпенем, меропенем, лефлоцин, амоксицилін/ клавуланат, фурагін, цефоперазон, цефтріаксон, які можна розглядати як препарат вибору для призначення стартової терапії ІСШ. Необхідно здійснювати постійний моніторинг за резистентністю до дії антибіотиків. Політику використання антибіотиків у кожному стаціонарі слід визначати залежно від локальних даних щодо резистентності до протимікробних препаратів.

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STUDIES ON SOME BACTERIAL DISEASES IN OREOCHROMIS NILOTICUS WITH SPECIAL REFERENCES TO TREATMENT

Mansoura Veterinary Medical Journal ◽

10.35943//mvmj.2018.19.1414 ◽

2018 ◽

pp. 39-47

Author(s):

Viola Zaki ◽

Ahmed EL-gamal ◽

Yasmin Reyad

Keyword(s):

Oreochromis Niloticus ◽

Bacterial Infections ◽

Antimicrobial Agents ◽

Histopathological Examination ◽

Klebsiella Oxytoca ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Tissue Samples ◽

Hydropic Degeneration ◽

Total Prevalence

he present research carried out to study the common bacterial infections in Oreochromis niloticus (Nile tilapia) in Manzala area at Dakahlia governorate and possible antimicrobial agents used for treatment. A total number of 400 fish were randomly collected from Manzala private farms at Dakahlia governorate and subjected to the clinical, bacteriological and histopathological examination. The highest prevalence of bacterial isolates during the whole period of examination of naturally infected O.niloticus was recorded for A.hydrophila (22.66%), followed by V.alginolyticus (19.01%), V.parahemolyticus (13.80%), Streptococcus spp. (12.24%), A.caviae (11.72%), V.cholera (10.16%), A.salmonicida (7.55%), while the lowest prevalence was recorded for Klebsiella oxytoca (2.86%). The seasonal highest total prevalence of bacterial isolates from examined naturally infected O. niloticus was recorded in spring (30.21%), followed by autumn (28.39%), then summer (22.40%) and the lowest prevalence was recorded in winter (19.01%). Histopathological findings of the tissue samples which collected from different organs of naturally infected O.niloticus revealed that spleen show marked hemosiderosis and sever hemorrhage, gills showsever congestion of lamellar capillaries with marked aneurysm, necrosis and hemorrhage of lamellar epithelium and liver show sever hydropic degeneration and necrosis of hepatocytes, Ciprofloxacin was the most effective antibiotic against all isolated bacterial strains

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Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis

Brazilian Journal of Microbiology ◽

10.1007/s42770-021-00445-y ◽

2021 ◽

Author(s):

Jesús Silva-Sanchez ◽

Humberto Barrios-Camacho ◽

Emmanuel Hernández-Rodriguez ◽

Josefina Duran-Bedolla ◽

Alejandro Sanchez-Perez ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Molecular Characterization ◽

Bovine Mastitis

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Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens

Antibiotics ◽

10.3390/antibiotics10050594 ◽

2021 ◽

Vol 10 (5) ◽

pp. 594

Author(s):

Sydney E. Schnur ◽

Raghavendra G. Amachawadi ◽

Giovanna Baca ◽

Sarah Sexton-Bowser ◽

Davina H. Rhodes ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Phenolic Compounds ◽

Enterococcus Faecalis ◽

Campylobacter Jejuni ◽

Bacterial Pathogens ◽

Klebsiella Oxytoca ◽

Bovine Mastitis ◽

Campylobacter Coli ◽

Foodborne Illnesses ◽

Phenolic Extract

Antimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds, and the grain extract may have the potential as a natural antimicrobial alternative. The study’s objective was to determine antimicrobial effects of sorghum phenolic extract on bacterial pathogens that cause bovine mastitis and human foodborne illnesses. Bacterial pathogens tested included Escherichia coli, Salmonella Typhimurium, Campylobacter jejuni, Campylobacter coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Klebsiella oxytoca, Staphylococcus aureus, and Enterococcus faecalis. Antibacterial activities of sorghum phenolic extracts were determined by agar-well diffusion assay. Sorghum phenolic extract was added to the wells in concentrations of 0, 100, 200, 500, 1000, or 4000 µg/mL. The control wells did not receive phenolic extract. Plates were incubated for 18–24 h, and the diameter of each zone of inhibition was measured. The results indicated that sorghum phenolic extract had inhibitory effects on Staphylococcus aureus, Enterococcus faecalis, Campylobacter jejuni, and Campylobacter coli.

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Asociación en un mismo plásmido de blaVIM-1 y qnrS2 en Klebsiella pneumoniae y Klebsiella oxytoca aisladas en Sevilla

Enfermedades Infecciosas y Microbiología Clínica ◽

10.1016/j.eimc.2011.09.020 ◽

2012 ◽

Vol 30 (5) ◽

pp. 246-248

Author(s):

José M. Rodríguez-Martínez ◽

M. Carmen Conejo ◽

Paula Díaz de Alba ◽

Lorena López-Cerero ◽

Pedro Fernandez-Echauri ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Klebsiella Oxytoca

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Piperacillin Tazobactam Compared with Co-Amoxiclav, Ampicillin plus Sulbactam and Timentin against Beta-Lactamase-Producing Clinical Isolates of Escherichia coli, Klebsiella pneumoniae and Klebsiella oxytoca

Chemotherapy ◽

10.1159/000239366 ◽

1995 ◽

Vol 41 (5) ◽

pp. 345-352 ◽

Cited By ~ 1

Author(s):

Walter H. Traub ◽

Birgit Leonhard

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Klebsiella Oxytoca ◽

Clinical Isolates ◽

Beta Lactamase

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An Important Public Health Problem "Carbapenem Resistant Enterobacteriaceae Frequency": An Example From A Training And Research Hospital

Gevher Nesibe Journal, IESDR ◽

10.46648/gnj.256 ◽

2021 ◽

Vol 6 (14) ◽

pp. 51-55

Author(s):

Ülkü VERANYURT ◽

Betül AKALIN

Keyword(s):

Public Health ◽

Klebsiella Pneumoniae ◽

Klebsiella Oxytoca ◽

Public Health Problem ◽

Important Public Health Problem ◽

Klebsiella Species ◽

Research Hospital ◽

Control Programs ◽

Carbapenem Resistant ◽

Training And Research

Background: Carbapenem-resistant Enterobacterales (CRE) infections are a significant threat to public health due to the limited availability of antibiotics and the effect on mortality. This study was conducted retrospectively to determine the prevalence of CRE in a teaching and research hospital in Istanbul. Materials and Methods: In 2016, 2017 and in the first half of 2018 Klebsiella species were evaluated retrospectively in culture samples that were sent to Microbiology Laboratory in an educational hospital. The typing of Klebsiella species were performed with MALDITOF-MS device (Biomerieux, France). Imipenem, merapenem, ertapenem susceptibilities of the strains were evaluated with VITEC2 Compact (Biomerieux, France) according to EUCAST (European Committee on Antimicrobial Susceptibility Testing). If the strains were found to be resistant, the results were confirmed by the antibiotic gradient test. Results: In our study, 257 samples; Klebsiella oxytoca 9 (3.49%) and Klebsiella pneumoniae 248 (96.51%) were detected. 130 endotracheal aspirates, 57 wounds, 34 blood, 15 urine, 9 sputum, 3 catheters, 4 tissue biopsies, 2 mediastinum, 2 peritoneal fluid and 1 pleural fluid strains were identified. While none of the Klebsiella oxytoca strains were resistant to carbapenems, the percentages of Klebsiella pneumoniae resistance against imipenem, merapenem and ertapenem were found to be 23.29%, 16.94% and 29.44% respectively. Conclusion: The increasing problem of carbapenem-resistant (CR) Klebsiella pneumoniae in the last decade has been observed in our hospital for the last 2 years. CR strains often show increased resistance to other antibiotics and their treatment possibilities are limited. It increases the importance of controlling this factor. The application of effective infection control programs and the use of rational antibiotics are of great importance.

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CMY-31 and CMY-36 Cephalosporinases Encoded by ColE1-Like Plasmids

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01284-08 ◽

2008 ◽

Vol 53 (3) ◽

pp. 1256-1259 ◽

Cited By ~ 24

Author(s):

A. Zioga ◽

J. M. Whichard ◽

S. D. Kotsakis ◽

L. S. Tzouvelekis ◽

E. Tzelepi ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Salmonella Enterica ◽

Klebsiella Oxytoca ◽

Citrobacter Freundii

ABSTRACT Two CMY-2 derivatives, CMY-31 (Gln215→Arg) from Salmonella enterica serotype Newport and CMY-36 (Ala77→Cys and Gln193→Glu) from Klebsiella pneumoniae, were characterized. Both cephalosporinases functionally resembled CMY-2. bla CMY alleles occurred as parts of a putative transposon comprising ISEcp1B and a Citrobacter freundii-derived sequence carried by ColE1-like plasmids similar to CMY-5-encoding pTKH11 from Klebsiella oxytoca.

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