scholarly journals Importance of biopsies and histological evaluation in patients with chronic diarrhea and normal colonoscopies

2014 ◽  
Vol 27 (3) ◽  
pp. 184-187 ◽  
Author(s):  
Franciane Mayra Nicoli KAGUEYAMA ◽  
Fernanda Michely NICOLI ◽  
Mauro Willemann BONATTO ◽  
Ivan Roberto Bonotto ORSO
Keyword(s):  
Differential Diagnosis ◽  
Terminal Ileum ◽  
Chronic Diarrhea ◽  
Collagenous Colitis ◽  
Normal Mucosa ◽  
Cross Sectional Study ◽  
Lymphoid Hyperplasia ◽  
Histological Evaluation ◽  
Cross Sectional ◽  
Eosinophilic Colitis

BACKGROUND: In patients with chronic diarrhea, colonoscopy may identify inflammatory causes or some occult disease, and also can show a normal mucosa. Serial biopsies of intestinal mucosa can be useful for a differential diagnosis, and to modify the treatment. AIM: To evaluate whether the biopsies performed in patients with chronic diarrhea and a normal colonoscopy contribute to the differential diagnosis and alter the therapeutic approach. METHODS: A descriptive, retrospective and cross-sectional study using a computerized database was done. Patients with chronic diarrhea and a normal colonoscopy underwent serial biopsies of the terminal ileum, ascending colon and rectum. RESULTS: From 398 records, 214 were excluded. Of the 184 patients enrolled, 91 showed histological changes: 40% nonspecific inflammation; 5.18% lymphocytic inflammation, 10.37% eosinophilic inflammation; 39.26% lymphoid hyperplasia; 2.22% collagenous colitis; 2.22% melanosis; and 0.74% pseudomelanose. The sites with the largest number of changes were the terminal ileum and right colon. CONCLUSIONS: Serial biopsies in patients with chronic diarrhea and normal colonoscopy identified changes in almost 50% of cases and 22% of these cases may had modified the treatment after identification of collagenous, lymphocytic and eosinophilic colitis.

Original Article: MicroRNA Dysregulation in the Gastric Carcinogenesis Cascade: Can We Anticipate Its Role in Individualized Care?

Pathobiology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Inês Pita ◽  
Diogo Libânio ◽  
Francisca Dias ◽  
Ana Luísa Teixeira ◽  
Inês Nogueira ◽  
...  
Keyword(s):  
Tissue Expression ◽  
Normal Mucosa ◽  
Cross Sectional Study ◽  
Gastric Carcinogenesis ◽  
Endoscopic Biopsy ◽  
Cross Sectional ◽  
Oncogenic Potential ◽  
Individualized Care ◽  
Gastric Neoplasia ◽  
Microrna Dysregulation

<b><i>Background:</i></b> Gastric carcinogenesis progresses from normal mucosa, atrophic/metaplastic gastritis, and dysplasia to adenocarcinoma. MicroRNAs (miRNAs) regulate DNA expression and have been implicated; however, their role is not fully established. <b><i>Aims:</i></b> The aim of this study was to characterize plasma and tissue expression of several miRNAs in gastric carcinogenesis stages. <b><i>Methods:</i></b> Single-center cross-sectional study in 64 patients: 19 controls (normal mucosa); 15 with extensive atrophic/metaplastic gastritis; and 30 with early gastric neoplasia (EGN). Seven miRNAs (miR-21, miR-146a, miR-181b, miR-370, miR-375, miR 181b, and miR-490) were quantified by real time-qPCR in peripheral blood and endoscopic biopsy samples. <b><i>Results:</i></b> We found a significant upregulation of miR-181b, miR-490, and miR-21 in the EGN mucosa (overexpression 2–14-times higher than controls). We observed a significant underexpression of miR-146a and miR-370 in atrophic/metaplastic gastritis (86 and 66% decrease, <i>p</i> = 0.008 and <i>p</i> = 0.001) and in EGN (89 and 62% reduction, <i>p</i> = 0.034 and <i>p</i> = 0.032) compared with controls. There were no differences between lesions and nonneoplastic mucosa and no dysregulation of plasma miRNAs. <b><i>Conclusion:</i></b> We found significant dysregulation of 5 miRNAs in gastric carcinogenesis, suggesting a tumor suppressor role for miR-146a and miR-370 and oncogenic potential for miR-21, miR-181, and miR-490. These changes happen diffusely in the gastric mucosa, suggesting a high-risk field defect, which may influence these patients’ surveillance.

scholarly journals Terminal ileum of patients who underwent colonoscopy: endoscopic, histologic and clinical aspects

2009 ◽  
Vol 46 (2) ◽  
pp. 102-106 ◽  
Author(s):  
Marcelo Maia Caixeta de Melo ◽  
Patrícia Maluf Cury ◽  
Luiz Sérgio Ronchi ◽  
Francisco de Assis Gonçalves-Filho ◽  
Geni Satomi Cunrath ◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Terminal Ileum ◽  
Chronic Diarrhea ◽  
Clinical Importance ◽  
Histological Evaluation ◽  
Clinical Aspects ◽  
Ileal Mucosa ◽  
Histological Alterations ◽  
Kappa Test ◽  
A Prospective Study

CONTEXT: For the diagnosis of the diseases which affect the terminal ileum, the colonoscopy allows macroscopic evaluation and the performing of biopsies. Studies with criteria for the endoscopic and histological characterization of this segment are scarce and there are still some doubts about the need of biopsies in patients with normal ileoscopy. OBJECTIVE: Study the terminal ileum of patients who underwent colonoscopy considering: endoscopic and histological correlation; agreement between results of the initial histological evaluation and slides review, and the chance of subjects with normal ileoscopy with abdominal pain and/or chronic diarrhea to show histological alterations. METHODS: In a prospective study, 111 patients who presented smooth mucosa without enanthema in the endoscopic exam of the terminal ileum were selected. Biopsies of the ileal mucosa of such patients were performed, being the slides routinely examined and reviewed afterwards. RESULTS: The correlation between patients with normal ileoscopy and ileum with preserved histological architecture was of 99.1%. The agreement between initial histological evaluation and slides review calculated by the Kappa test was 0.21. In patients with abdominal pain and/or chronic diarrhea, the chance of showing histological alterations was 2.5 times higher than the others. CONCLUSIONS: The correlation between endoscopic and histological findings was high. The agreement between the initial histologic evaluation and slides review was not satisfactory. The chance of subjects with normal ileoscopy with abdominal pain and/or chronic diarrhea, showing histological alterations was higher in relation to the asymptomatic ones or with other symptoms, although the clinical importance of this datum was not evaluated.

scholarly journals VAGINAL SONOGRAPHY a HYSTEROSCOPY

2009 ◽  
Vol 16 (03) ◽  
pp. 432-437
Author(s):  
MASOMEH ASGHARNIA ◽  
Zahra Mohammad Tabar ◽  
MARZIEH MEHRAFZA ◽  
Mary am Shakiba ◽  
MONA OUDI ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Uterine Cavity ◽  
Cross Sectional Study ◽  
Therapeutic Modality ◽  
Histological Evaluation ◽  
Complete Evaluation ◽  
Cross Sectional ◽  
Predictive Values ◽  
Infertile Women ◽  
Sensitivity Specificity

B a c k g r o u n d : Hysteroscopy is a valuable diagnostic and therapeutic modality in the management of infertility. A i m : To evaluatethe consistency of hysteroscopy based on a histopathological report from endometrial specimens for intrauterine disorders. Materials andMethods: This is a cross-sectional study. The study included 115 infertile patients. All were admitted for investigation of infertile women beforeassisted reproduction in Mehr infertility institute between 2006 and 2007 hysteroscopy, and histological evaluation of endometrial biopsyperformed.We compared the efficacy of hysteroscopy in the diagnosis of benign intrauterine pathology in infertile women in whom the diagnosiswas confirmed by histologic studies. The women had a complete evaluation with preoperative hysteroscopy, and histological analysis of uterinecavity specimens. Sensitivity, specificity, predictive and negative predictive values were calculated for hysteroscopy considering the histologicalstudy as 100%. Results: Sensitivity and specificity of sonography in diagnosing the polyp were stated 81 % and 64% respectively. Sensitivityand specificity of hysteroscopy showed of polyps revealed 85% and 84% respectively. The results indicated that Sensitivity and specificity ofsonography in diagnosing the myoma were 25% and 98% respectively. Sensitivity and specificity of hysteroscopy in diagnosing the myomawere expressed 50% and 93% respectively. C o n c l u s i o n : Hysteroscopy is a safe and rapid direct visualisation of the uterine cavity. We believeit should be replaced by the diagnostic hysteroscopy as a first line infertility investigation.

scholarly journals Differential diagnosis between chronic versus aggressive periodontitis and staging of aggressive periodontitis: A cross-sectional study

2017 ◽  
Vol 8 (4) ◽  
pp. 594 ◽  
Author(s):  
SrinivasSulugodu Ramachandra ◽  
VivekVijay Gupta ◽  
DhoomSingh Mehta ◽  
KalyanC Gundavarapu ◽  
Nibali Luigi
Keyword(s):  
Differential Diagnosis ◽  
Aggressive Periodontitis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional
Sign in / Sign up

Export Citation Format

Share Document