scholarly journals Comparison between eletrosurgery and cold blade in tongues of rats: a preliminary experimental study

2009 ◽  
Vol 24 (5) ◽  
pp. 362-366
Author(s):  
Belmiro Cavalcanti do Egito Vasconcelos ◽  
Lígia Helena Macedo de Freitas ◽  
Luciana Karla de Morais Santos ◽  
José Ricardo Dias Pereira ◽  
Riedel Frota ◽  
...  
Keyword(s):  
Experimental Study ◽  
Wistar Rats ◽  
Histological Study ◽  
Healing Process ◽  
Inflammatory Cells ◽  
The Other ◽  
Epithelial Proliferation ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Fibroblastic Proliferation

PURPOSE: To compare tissue cicatrization in the tongue of rats using electrosurgery and the cold blade. METHODS: Ten adult male Wistar rats were divided into two groups of 5 animals each, according to the time of sacrifice (3 and 7 days). Each animal had two incisions, one made with a cold blade and the other with an electric blade, both of which were approximately 0.5 mm in length. Following sacrifice of the animals, the tongues were submitted to a histological study in order to classify the presence of angiogenesis, fibroblastic proliferation, epithelial proliferation and inflammatory cells as good, moderate and weak. RESULTS: The wound made with electric blade presented a delay in the healing process and a greater inflammatory response as compared with the cold blade, despite the fact that there was no statistically significant difference (p<0.05). CONCLUSION: There were no significant statistical differences between electrosurgery and the cold blade in relation to angiogenesis, fibroblastic proliferation, epithelial proliferation or the presence of inflammatory cells at any time of evaluation.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals Infiltrasi Neutrofil pada Tikus dengan Periodontitis setelah Pemberian Ekstrak Etanolik Kulit Manggis

2014 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Rendra Chriestedy Prasetya ◽  
Nunuk Purwanti ◽  
Tetiana Haniastuti
Keyword(s):  
Inflammatory Disease ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Inflammatory Cells ◽  
Neutrophil Infiltration ◽  
Chronic Inflammatory Disease ◽  
Periodontopathic Bacteria ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Hematoxylin Eosin

Periodontitis adalah inflamasi kronis yang disebabkan oleh bakteri periodontopatogen. Pada periodontitis terjadi peningkatan infiltrasi neutrofil yang berfungsi untuk memfagositosis bakteri yang menginfiltrasi jaringan gingiva. Kulit manggis mempunyai bahan aktif yaitu xanton yang mempunyai efek antiinflamasi dengan jalan menghambat sintesis PGE2 sehingga akan menurunkan infiltrasi sel inflamasi. Penelitian ini bertujuan untuk mengetahui infiltrasi sel neutrofil pada periodontitis setelah pemberian ekstrak etanolik kulit manggis. Tikus wistar jantan sebanyak empat puluh delapan ekor diinduksi periodontitis dengan ligasi pada daerah subgingiva gigi anterior rahang bawah selama 7 hari. Setelah hari ke-7, ligasi dilepas selanjutnya tikus dibagi menjadi 4 kelompok perlakuan yaitu ekstrak kulit manggis 30 mg/kg BB dan 60 mg/kg BB, ibuprofen dan saline. Tikus dikorbankan pada hari ke-1, 3, 5 dan 7 setelah perlakuan. Jaringan pada bagian anterior rahang bawah ditanam dalam paraffin dan dilakukan pemotongan serial kemudian diwarnai dengan hematoxylin eosin. Jumlah neutrofil dihitung di bawah mikroskop dengan perbesaran 400x. Data jumlah neutrofil dianalisa dengan Two Way Anova. Hasil Two Way Anova menunjukkan perbedaan yang bermakna rerata sel neutrofil antar kelompok perlakuan (p<0,05) mengindikasikan pemberian ekstrak etanolik kulit manggis berpengaruh terhadap jumlah sel neutrofil. Dapat disimpulkan bahwa pemberian ekstrak etanolik kulit manggis mampu menurunkan infiltrasi sel neutrofil pada tikus yang diinduksi periodontitis.Neutrophil infiltration in rats with periodontitis after the granting of Ethanolic Extract Skin Mangosteen. Periodontitis is a chronic inflammatory disease caused by periodontopathic bacteria. When periodontitis occurs are followed by neutrophil infiltration. Mangosteen rind contains xanthone, an anti-inflammatory substance which inhibits the synthesis of PGE2 and therefore reduces inflammatory cells infiltration. This research aimed to study neutrophil cells infiltration in experimental-induced periodontitis rats after mangosteen rind ethanolic extract administration. Forty-eight male wistar rats were induced the periodontitis by putting silk ligature subgingivally around the neck of the anterior lower teeth for seven days. After the ligation had been taken out, the rats were divided into four groups and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen, and saline. The rats were sacrificed on the 1st, 3rd, 4th, seventh day after the treatment. Their anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with hematoxylin-eosin. The neutrophil cells were observed and counted under the microscope (400x). The data were analyzed using Anova. Anova result showed a significant difference among group (p<0,05) indicating that mangosteen rind ethanolic extract affected the number of neutrophils. In conclusion, mangosteen rind ethanolic extract reduced the number of neutrophil infiltration in periodontitis rats.

scholarly journals Effect of single clove garlic on clinical symptoms of photoaging in wistar rats exposed with UV-B

2019 ◽  
Vol 2 (2) ◽  
pp. 40-44
Author(s):  
Nyoman Pratiwi Hapsari Dewi ◽  
I Gusti Ayu Dewi Ratnayanti ◽  
I Gusti Kamasan Nyoman Arijana ◽  
Ni Made Linawati
Keyword(s):  
Clinical Symptom ◽  
Wistar Rats ◽  
Clinical Symptoms ◽  
Light Exposure ◽  
Premature Aging ◽  
The Other ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Photoaging is a premature aging that occurs on skin due to the ultraviolet light exposure that causes the emergence of clinical symptoms, one of which is wrinkle. One kind of material that can prevent photoaging is antioxidant. A single clove garlic has the highest antioxidant activity compared to the other materials. Aim: This research aimed at investigating the effectivity of single clove garlic in inhibiting the clinical symptom of photoaging. Method: This research was an experimental research, utilizing pre-post-test control group design. The used sample were 30 male wistar rats which were divided into 6 experimental groups. All groups were exposed to UV-B light with the amount of 840 mJ/cm2. Control group (P1) was only exposed to UV-B while the other groups were treated with placebo cream (P2), sunblock (P3), 5% garlic cream (P4), 10% garlic cream (P5) and 20% garlic cream (P6) respectively. The clinical symptom in the form of wrinkle was observed by using dermascope and the observations were categorized based on Glogau Scale. The statistical analysis utilized Wilcoxon and Kruskal Wallis test which was followed by Mann Whitney test. Result: The result of the research showed that there was significant difference on the apparent wrinkle on P1 group and P3, P5 and P6. Meanwhile the comparison between P1, P2 and P4 was not significant. Conclusion: The single clove garlic extract can prevent photoaging and has the similar protective effect for the skin as of sunblock.

scholarly journals Jumlah sel makrofag gingiva tikus wistar jantan yang diinduksi periodontitis setelah pemberian ekstrak etanolik kulit manggis Amount of machropahges cells in periodontitis-induced wistar rats after mangosteen rind etanolic extract administration

2013 ◽  
Vol 12 (3) ◽  
pp. 135
Author(s):  
Rendra Chriestedy Prasetya
Keyword(s):  
Wistar Rats ◽  
Inflammatory Cells ◽  
Anova Test ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Anti Inflammatory

Periodontitis is characterized with macrophages infiltration that is used to fagocyt bacteria which infiltrated atgingiva. Mangosteen rind contains xantone, an anti-inflammatory substance which inhibits the synthesis of PGE2 andtherefore reduce inflammatory cells infiltration. This research aimed to study machropahges cells infiltration inperiodontitis-induced wistar rats after administration of mangosteen rind etanolic extract. Forty eight male wistar ratswere induced periodontitis for 7 days. After the ligation was taken out, the rats were divided into 4 groups, and treatedorally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen and saline. The rats were sacrified on the 1st, 3rd, 4th, and 7th day after the treatment. Their anterior lower jaws were processed histologically and stained withhematoxylin eosin. Two-way anova test showed a significant difference among groups (p<0.05). It was concluded thatmangosteen rind etanolic extract reduced the number of macropaghes cells gingiva in periodontitis male wistar rats.

scholarly journals The Effects of Borax (NA2B4O710H2O) on Histopathology of Wistar Rats’ Cerebrum (Rattus norvegicus)

2021 ◽  
Vol 11 (3) ◽  
pp. 484-488
Author(s):  
Fernanda Elfan ◽  
Suryo Kuncorojakti ◽  
Nusdianto Triakoso
Keyword(s):  
Experimental Study ◽  
Statistical Analysis ◽  
Pyramidal Cell ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
The Other ◽  
Control Treatment ◽  
Cell Necrosis ◽  
Treatment Groups ◽  
Significant Difference

The present study aimed to determine the effects of borax (Na2B4O7.10H2O) addition on the changes of histological cerebrum imaging in the brains of white mice (Rattus norvegicus). The current research was an experimental study with randomization of 24 white mice that were divided into four treatment groups with five replications. Borax was dissolved for each treatment with a dose of 19 mg/mouse/day, 26 mg/mouse/day, and 37 mg/mouse/day, and it was administered orally for 14 days. Then, it was analyzed statistically using the Kruskal-Wallis test. The statistical analysis results suggested that there were significantly different results in each treatment group. The control treatment with an administration dose of 26 mg/rat/day had a significantly different result in the worst cloudy swelling degeneration of cerebrum in histopathology imaging on Wistar rats (Rattus norvegicus). Using the Mann-Whitney test, it was found that the dose of borax at 37 mg/rat/day led to significant difference, compared to the other treatment groups, which means that 37 mg/rat/day of borax caused the worst pyramidal cell necrosis in histopathology imaging of the cerebrum on white mice. Borax exposure on Wistar rats (Rattus norvegicus) can cause cloudy swelling at a dose of 26mg/head/day, and pyramidal cell necrosis at a dose of 37 mg/head/day.

scholarly journals EFFECT OF CHRONIC RENAL DYSFUNCTION ON THE PERMEABILITY OF THE COLON TO WATER AND ELECTROLYTES: EXPERIMENTAL STUDY IN RATS

2019 ◽  
Vol 32 (4) ◽  
Author(s):  
Elionai Gomes FREIRE ◽  
José Cirlânio Sousa ALBUQUERQUE ◽  
Israel Pinto LEAL ◽  
Nayara Alves SOUSA ◽  
José Ronaldo Vasconcelos da GRAÇA
Keyword(s):  
Experimental Study ◽  
Renal Failure ◽  
Polyethylene Glycol ◽  
Gastrointestinal Tract ◽  
Wistar Rats ◽  
The Other ◽  
Male Wistar Rats ◽  
Progression Of Renal Failure ◽  
Colonic Secretion ◽  
Secretory Capacity

ABSTRACT Background: Renal insufficiency is a disease that affects several organs by provoking hypervolemia and uremia. The disease reaches more than 500 million people worldwide and few studies bring their influence on the gastrointestinal tract. Aim: To evaluate the influence of 5/6 nephrectomy-induced hypervolemia on colonic permeability to water and electrolytes. Method: Sixty male Wistar rats weighing between 280-300 g were divided into three groups: 3, 7 and 14 days after nephrectomy, each one having a false-operated/control and partially nephrectomized. For colonic permeability they were submitted to colonic perfusion with a solution of Tyroad containing phenolphthalein. Differences among the concentrations of Na+, K+ and Cl- were used to calculate the rate of colonic permeability for the electrolytes. Phenolphthalein concentrations were used to evaluate the rate of secretion and water absorption. Results: The colonic secretion of water and electrolytes occurred expressively in the group seven days after nephrectomy. Hemodynamic and biochemical assessments determined the progression of renal failure in all three groups and polyethylene glycol was shown to be effective in reversing the secretory capacity of the colon. Conclusion: Hypervolemia established after 7 days post-nephrectomy 5/6 caused marked colonic secretion for water and electrolytes. The organism presents progressive colonic secretion as the blood volume increases; on the other hand, polyethylene glycol was able to revert this secretory framework of the colon to water and electrolytes by reversing the hypervolemia.

scholarly journals Effects of the hydrophilic extract of Juniperus excelsa on renal function in male Wistar rats

2018 ◽  
Vol 8 (1) ◽  
pp. 34-37
Author(s):  
Sadrollah Mehrabi
Keyword(s):  
Renal Function ◽  
Wistar Rats ◽  
Urine Volume ◽  
Inflammatory Cells ◽  
Control Group ◽  
Renal Tubules ◽  
Serum Samples ◽  
Juniperus Excelsa ◽  
Significant Difference ◽  
Male Wistar Rats

Introduction: Ores plant (Juniperus excelsa) has been used for a long-time in the treatment of kidney disease. Objectives: The aim of this study was to investigate the effects of J. excelsa extract on renal function in male Wistar rats. Materials and Methods: In this study, 32 male Wistar rats were randomly assigned into four groups of eight rats. Distilled water was used for the healthy control group and the other three groups received doses of 10%, 25% and 50% of the extract for one month. Prior to the intervention and on the 15th and 30th days after intervention, 24-hour urine was collected for measurement of protein, creatinine, and urine volume. On the 30th day, the rats were anesthetized with ether and in addition to the urinary samples, serum samples were taken directly from their heart to check for creatinine, urea, sodium, and potassium. Additionally, both kidneys were removed and examined for histological changes. Results: There was a significant difference between the groups before and after intervention regarding creatinine clearance (P=0.008). The mean serum urea on the 15th and 30th days of study was respectively 93±37.33 and 86.47±71.07 mg/dL (P=0.001). In pathology examination, minimal infiltration of inflammatory cells in the interstitium and mild decrease in thickness of renal tubules was observed in 50% dose of the extract. Conclusion: This study showed that the greatest impact of J. excelsa on the renal function of the male Wistar rats was in doses of 50% of the extracts.

scholarly journals ANÁLISE MORFOLÓGICA DAS VIAS AÉREAS DE RATOS EXPOSTOS À FUMAÇA DO CIGARRO, E AO ÁLCOOL E SUA RELAÇÃO COM O EXERCÍCIO FÍSICO

2020 ◽  
Vol 12 (2) ◽  
pp. 35-41
Author(s):  
Fabiola de Azevedo Mello ◽  
Carolina Capelasso de Oliveira ◽  
Gabriela Vidotto Cavalieri ◽  
Armando Ribeiro Florido Neto ◽  
Lucas Agostini ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Alcohol Intake ◽  
Inflammatory Infiltrate ◽  
Inflammatory Cells ◽  
Respective Group ◽  
Physical Exercises ◽  
Significant Difference ◽  
Male Wistar Rats

The aim of the study was to quantify and compare, morphologically, the mucus and inflammatory infiltrate in rats exposed to cigarette smoke, alcohol and both agents, including physical exercise. Forty male Wistar rats were used, randomly divided into four groups. Control animals performed physical exercises. The other animals were exposed to cigarette smoke, alcohol intake and physical training, according to the respective group. The lungs of the animals were collected to assess the findings. There was no significant difference between the groups regarding the assessment of the airway perimeter and the amount of inflammatory cells in the animals' airways. It was concluded that there was no influence of physical exercise on the production of mucus and inflammatory infiltrate in rats exposed to alcohol and cigarette smoke. Thus, it is suggested to carry out further studies in order to elucidate the results.

scholarly journals Neutrophil count in the gingival wound healing process after apitoxin gel application (gingival wound healing model on Wistar rats)

2021 ◽  
Vol 6 (2) ◽  
pp. 65
Author(s):  
Urfa Tabtila ◽  
Swastiana Eka Yunita ◽  
Muhammad Nabil Pratama ◽  
Juni Handajani
Keyword(s):  
Wound Healing ◽  
Periodontal Disease ◽  
Neutrophil Count ◽  
Wistar Rats ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Punch Biopsy ◽  
Bee Venom ◽  
Wound Healing Process ◽  
Male Wistar Rats

Periodontal disease has a high prevalence in Indonesia. Gingival disease is a periodontal disease if it is severe, requires surgical therapy called a gingivectomy. Surgical periodontal procedures lead to a gingival wound that is usually treatedusing a periodontal pack. Periodontal pack has no curative property in general but assists in a wound healing process by protecting tissues. Neutrophils are inflammatory cells that act as a host’s first defense against infectious pathogens and they are important in wound healing. Wound healing can be accelerated by apitoxin gel from honeybee Apis mellifera species. The aim of this study was to determine the effectiveness of apitoxin gel on neutrophil count on gingival woundhealing process. Medical bee venom powder (Umea, Sweden) was mixed with double-distilled water to prepare a concentration of 0.01% bee venom. The solution was mixed with 10% propylene glycol, 0.01% methylparaben, and3% CMC-sodium. Thirty six gingiva of 3 month-old male Wistar rats (weighing 200-250 gram) were injured using punch biopsy (3 mm in diameter). They were treated in 4 groups: apitoxin gel, apitoxin gel with a periodontal pack, NSAIDsFlamar® gel with periodontal pack, and a periodontal pack. Three Wistar rats from each group were euthanized and their jaws were taken after 48 hours treatment to make histology slides with Hematoxylin Eosin (HE) staining. Neutrophil counts were observed. The data were analyzed using Anova test with 95% confidence level. The results showed there were significan defferebces among 4 groups (p<0.05). The highest neutrophil count on the 2nd day was found in the apitoxin gel induced the 2 day of gingival wound healing process.

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