scholarly journals Jumlah sel makrofag gingiva tikus wistar jantan yang diinduksi periodontitis setelah pemberian ekstrak etanolik kulit manggis Amount of machropahges cells in periodontitis-induced wistar rats after mangosteen rind etanolic extract administration

2013 ◽  
Vol 12 (3) ◽  
pp. 135
Author(s):  
Rendra Chriestedy Prasetya
Keyword(s):  
Wistar Rats ◽  
Inflammatory Cells ◽  
Anova Test ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Anti Inflammatory

Periodontitis is characterized with macrophages infiltration that is used to fagocyt bacteria which infiltrated atgingiva. Mangosteen rind contains xantone, an anti-inflammatory substance which inhibits the synthesis of PGE2 andtherefore reduce inflammatory cells infiltration. This research aimed to study machropahges cells infiltration inperiodontitis-induced wistar rats after administration of mangosteen rind etanolic extract. Forty eight male wistar ratswere induced periodontitis for 7 days. After the ligation was taken out, the rats were divided into 4 groups, and treatedorally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen and saline. The rats were sacrified on the 1st, 3rd, 4th, and 7th day after the treatment. Their anterior lower jaws were processed histologically and stained withhematoxylin eosin. Two-way anova test showed a significant difference among groups (p<0.05). It was concluded thatmangosteen rind etanolic extract reduced the number of macropaghes cells gingiva in periodontitis male wistar rats.

scholarly journals Infiltrasi Neutrofil pada Tikus dengan Periodontitis setelah Pemberian Ekstrak Etanolik Kulit Manggis

2014 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
Rendra Chriestedy Prasetya ◽  
Nunuk Purwanti ◽  
Tetiana Haniastuti
Keyword(s):  
Inflammatory Disease ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Inflammatory Cells ◽  
Neutrophil Infiltration ◽  
Chronic Inflammatory Disease ◽  
Periodontopathic Bacteria ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Hematoxylin Eosin

Periodontitis adalah inflamasi kronis yang disebabkan oleh bakteri periodontopatogen. Pada periodontitis terjadi peningkatan infiltrasi neutrofil yang berfungsi untuk memfagositosis bakteri yang menginfiltrasi jaringan gingiva. Kulit manggis mempunyai bahan aktif yaitu xanton yang mempunyai efek antiinflamasi dengan jalan menghambat sintesis PGE2 sehingga akan menurunkan infiltrasi sel inflamasi. Penelitian ini bertujuan untuk mengetahui infiltrasi sel neutrofil pada periodontitis setelah pemberian ekstrak etanolik kulit manggis. Tikus wistar jantan sebanyak empat puluh delapan ekor diinduksi periodontitis dengan ligasi pada daerah subgingiva gigi anterior rahang bawah selama 7 hari. Setelah hari ke-7, ligasi dilepas selanjutnya tikus dibagi menjadi 4 kelompok perlakuan yaitu ekstrak kulit manggis 30 mg/kg BB dan 60 mg/kg BB, ibuprofen dan saline. Tikus dikorbankan pada hari ke-1, 3, 5 dan 7 setelah perlakuan. Jaringan pada bagian anterior rahang bawah ditanam dalam paraffin dan dilakukan pemotongan serial kemudian diwarnai dengan hematoxylin eosin. Jumlah neutrofil dihitung di bawah mikroskop dengan perbesaran 400x. Data jumlah neutrofil dianalisa dengan Two Way Anova. Hasil Two Way Anova menunjukkan perbedaan yang bermakna rerata sel neutrofil antar kelompok perlakuan (p<0,05) mengindikasikan pemberian ekstrak etanolik kulit manggis berpengaruh terhadap jumlah sel neutrofil. Dapat disimpulkan bahwa pemberian ekstrak etanolik kulit manggis mampu menurunkan infiltrasi sel neutrofil pada tikus yang diinduksi periodontitis.Neutrophil infiltration in rats with periodontitis after the granting of Ethanolic Extract Skin Mangosteen. Periodontitis is a chronic inflammatory disease caused by periodontopathic bacteria. When periodontitis occurs are followed by neutrophil infiltration. Mangosteen rind contains xanthone, an anti-inflammatory substance which inhibits the synthesis of PGE2 and therefore reduces inflammatory cells infiltration. This research aimed to study neutrophil cells infiltration in experimental-induced periodontitis rats after mangosteen rind ethanolic extract administration. Forty-eight male wistar rats were induced the periodontitis by putting silk ligature subgingivally around the neck of the anterior lower teeth for seven days. After the ligation had been taken out, the rats were divided into four groups and treated orally with mangosteen rind extract 60 mg/kg BB, 30 mg/kg BB, ibuprofen, and saline. The rats were sacrificed on the 1st, 3rd, 4th, seventh day after the treatment. Their anterior lower jaws were processed for paraffin embedded tissue, cut serially and stained with hematoxylin-eosin. The neutrophil cells were observed and counted under the microscope (400x). The data were analyzed using Anova. Anova result showed a significant difference among group (p<0,05) indicating that mangosteen rind ethanolic extract affected the number of neutrophils. In conclusion, mangosteen rind ethanolic extract reduced the number of neutrophil infiltration in periodontitis rats.

scholarly journals Naringin and Hesperidin Counteract Diclofenac-Induced Hepatotoxicity in Male Wistar Rats via Their Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Rasha A. Hassan ◽  
Walaa G. Hozayen ◽  
Haidy T. Abo Sree ◽  
Hessah M. Al-Muzafar ◽  
Kamal A. Amin ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Liver Lipid ◽  
Diclofenac Sodium ◽  
Elevated Serum ◽  
Inflammatory Cells ◽  
Hepatic Necrosis ◽  
Hydropic Degeneration ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
Anti Inflammatory

This study is aimed at evaluating the preventive effect and at suggesting the mode of actions of naringin and hesperidin and their combination in diclofenac-induced hepatotoxicity. Male Wistar rats, intraperitoneally injected with diclofenac sodium (3 mg/kg b.wt/day), were orally treated with naringin (20 mg/kg b.wt/day) and hesperidin (20 mg/kg b.wt/day) and their combination for 4 weeks. The administrations of naringin and hesperidin to diclofenac-injected rats led to a significant decrease in the elevated serum ALT, AST, LDH, ALP, GGT, total bilirubin, TNF-α, and IL-17 levels as well as liver lipid peroxidation and liver p53 and caspase-3 mRNA expressions. In contrast, serum IL-4 level, liver GSH content, and liver GPx and SOD activities increased. In association, diclofenac-induced deleterious histological alterations including hydropic degeneration, cytoplasmic vacuolization, apoptosis, and focal hepatic necrosis of hepatocytes associated with inflammatory cells’ infiltration were remarkably improved by treatments with naringin and hesperidin. In conclusion, naringin, hesperidin, and their combination, which was the most potent, counteract diclofenac-induced liver injury via antioxidant, anti-inflammatory, and antiapoptotic actions. Thus, this study recommends the use of naringin and hesperidin or their combination to resolve the side effects of drugs like diclofenac on the liver.

scholarly journals Effects of the hydrophilic extract of Juniperus excelsa on renal function in male Wistar rats

2018 ◽  
Vol 8 (1) ◽  
pp. 34-37
Author(s):  
Sadrollah Mehrabi
Keyword(s):  
Renal Function ◽  
Wistar Rats ◽  
Urine Volume ◽  
Inflammatory Cells ◽  
Control Group ◽  
Renal Tubules ◽  
Serum Samples ◽  
Juniperus Excelsa ◽  
Significant Difference ◽  
Male Wistar Rats

Introduction: Ores plant (Juniperus excelsa) has been used for a long-time in the treatment of kidney disease. Objectives: The aim of this study was to investigate the effects of J. excelsa extract on renal function in male Wistar rats. Materials and Methods: In this study, 32 male Wistar rats were randomly assigned into four groups of eight rats. Distilled water was used for the healthy control group and the other three groups received doses of 10%, 25% and 50% of the extract for one month. Prior to the intervention and on the 15th and 30th days after intervention, 24-hour urine was collected for measurement of protein, creatinine, and urine volume. On the 30th day, the rats were anesthetized with ether and in addition to the urinary samples, serum samples were taken directly from their heart to check for creatinine, urea, sodium, and potassium. Additionally, both kidneys were removed and examined for histological changes. Results: There was a significant difference between the groups before and after intervention regarding creatinine clearance (P=0.008). The mean serum urea on the 15th and 30th days of study was respectively 93±37.33 and 86.47±71.07 mg/dL (P=0.001). In pathology examination, minimal infiltration of inflammatory cells in the interstitium and mild decrease in thickness of renal tubules was observed in 50% dose of the extract. Conclusion: This study showed that the greatest impact of J. excelsa on the renal function of the male Wistar rats was in doses of 50% of the extracts.

scholarly journals ANÁLISE MORFOLÓGICA DAS VIAS AÉREAS DE RATOS EXPOSTOS À FUMAÇA DO CIGARRO, E AO ÁLCOOL E SUA RELAÇÃO COM O EXERCÍCIO FÍSICO

2020 ◽  
Vol 12 (2) ◽  
pp. 35-41
Author(s):  
Fabiola de Azevedo Mello ◽  
Carolina Capelasso de Oliveira ◽  
Gabriela Vidotto Cavalieri ◽  
Armando Ribeiro Florido Neto ◽  
Lucas Agostini ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Alcohol Intake ◽  
Inflammatory Infiltrate ◽  
Inflammatory Cells ◽  
Respective Group ◽  
Physical Exercises ◽  
Significant Difference ◽  
Male Wistar Rats

The aim of the study was to quantify and compare, morphologically, the mucus and inflammatory infiltrate in rats exposed to cigarette smoke, alcohol and both agents, including physical exercise. Forty male Wistar rats were used, randomly divided into four groups. Control animals performed physical exercises. The other animals were exposed to cigarette smoke, alcohol intake and physical training, according to the respective group. The lungs of the animals were collected to assess the findings. There was no significant difference between the groups regarding the assessment of the airway perimeter and the amount of inflammatory cells in the animals' airways. It was concluded that there was no influence of physical exercise on the production of mucus and inflammatory infiltrate in rats exposed to alcohol and cigarette smoke. Thus, it is suggested to carry out further studies in order to elucidate the results.

scholarly journals Comparison between eletrosurgery and cold blade in tongues of rats: a preliminary experimental study

2009 ◽  
Vol 24 (5) ◽  
pp. 362-366
Author(s):  
Belmiro Cavalcanti do Egito Vasconcelos ◽  
Lígia Helena Macedo de Freitas ◽  
Luciana Karla de Morais Santos ◽  
José Ricardo Dias Pereira ◽  
Riedel Frota ◽  
...  
Keyword(s):  
Experimental Study ◽  
Wistar Rats ◽  
Histological Study ◽  
Healing Process ◽  
Inflammatory Cells ◽  
The Other ◽  
Epithelial Proliferation ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Fibroblastic Proliferation

PURPOSE: To compare tissue cicatrization in the tongue of rats using electrosurgery and the cold blade. METHODS: Ten adult male Wistar rats were divided into two groups of 5 animals each, according to the time of sacrifice (3 and 7 days). Each animal had two incisions, one made with a cold blade and the other with an electric blade, both of which were approximately 0.5 mm in length. Following sacrifice of the animals, the tongues were submitted to a histological study in order to classify the presence of angiogenesis, fibroblastic proliferation, epithelial proliferation and inflammatory cells as good, moderate and weak. RESULTS: The wound made with electric blade presented a delay in the healing process and a greater inflammatory response as compared with the cold blade, despite the fact that there was no statistically significant difference (p<0.05). CONCLUSION: There were no significant statistical differences between electrosurgery and the cold blade in relation to angiogenesis, fibroblastic proliferation, epithelial proliferation or the presence of inflammatory cells at any time of evaluation.

scholarly journals Effect of fenofibrate on acute and subacute inflammation in male Wistar rats

2018 ◽  
Vol 7 (11) ◽  
pp. 2190
Author(s):  
Serah J. ◽  
Torgal S. S. ◽  
Ramesh C. ◽  
Anna M.
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Histopathological Examination ◽  
Dry Weight ◽  
Study Groups ◽  
Paw Oedema ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Mean ◽  
Anti Inflammatory

Background: The objective of the study was to investigate the effect of fenofibrate on acute and subacute models of inflammation in adult male Wistar rats.Methods: After obtaining clearance from Institutional Animal Ethics Committee, six adult male Wistar rats were allocated to each of the three groups i.e. control, aspirin and fenofibrate. Acute inflammation was studied using carrageenan induced rat paw oedema and the volume displacement due to paw oedema was measured using the plethysmograph. Subacute inflammation was studied using foreign body insertion (cotton pellet and grass pith) models. Dry granuloma weight and histopathological examination of the granuloma were the outcome measures for measuring subacute inflammation. The percentage inhibition of inflammation with aspirin and fenofibrate was calculated in both acute and subacute models. The experiments were conducted according to the guidelines of the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA). The mean volume displacement obtained with a plethysmograph, the mean dry weight of granuloma and the percentage inhibition with aspirin and fenofibrate were analyzed by one way analysis of variance (ANOVA) using Graph pad prism software.Results: Aspirin and fenofibrate significantly reduced both acute and subacute inflammation (p<0.001). Dunnet’s test showed a significant difference in the study groups when compared to the control. The reduction of inflammation with fenofibrate was comparable to aspirin.Conclusions: Oral fenofibrate showed significant anti-inflammatory activity, which was comparable to aspirin, in both acute as well as sub-acute models of inflammation. This anti-inflammatory effect may benefit atherosclerosis in patients receiving fenofibrate for hyperlipidemia.

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

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