scholarly journals Effects of bromopride on abdominal wall healing with induced peritoneal sepsis after segmental colectomy and colonic anastomosis in rats

2011 ◽  
Vol 26 (6) ◽  
pp. 433-437 ◽  
Author(s):  
Marcos Vinícius Melo de Oliveira ◽  
Alexandre Malta Brandão ◽  
Pedro Henrique Alves de Morais ◽  
Naiara Galvão da Silva ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Saline Solution ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Left Colon ◽  
Control Group ◽  
The Third ◽  
Segmental Colectomy ◽  
Group B ◽  
Harmful Effects

PURPOSE: Evaluate the effects of bromopride on abdominal wall healing of rats with induced peritoneal sepsis after segmental colectomy and colonic anastomosis. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of bromopride (bromopride group - B) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (GB3 and GC3) or seventh postoperative day (GB7 and GC7). It was analyzed the following characteristics: breaking strength of the abdominal wall's wound; surgical and histopathological features of the abdominal wall; and clinical features of the rats. RESULTS: There was no difference between the groups in relation to the weight of the rats and the breaking strength of the abdominal wall's wound. The GB7 group presented less edema and less quantity of fibrin during histopathological evaluation compared to the GC7 group. CONCLUSION: Bromopride did not have harmful effects on the healing of abdominal wall in rats.

scholarly journals Does metoclopramide impair anastomotic healing of the left colon of rats?

2011 ◽  
Vol 26 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Silvana Marques e Silva ◽  
Vânia Maria Moraes Ferreira ◽  
Omar Feres ◽  
Paulo Gonçalves de Oliveira ◽  
João Batista de Sousa
Keyword(s):  
Saline Solution ◽  
Breaking Strength ◽  
Anastomotic Dehiscence ◽  
Left Colon ◽  
Control Group ◽  
Colonic Anastomoses ◽  
Intestinal Anastomoses ◽  
Hydroxyproline Concentration ◽  
Harmful Effects ◽  
Experimental Group

PURPOSE: To evaluate the effects of metoclopramide on the formation of adhesion and the healing of left colonic anastomoses in rats. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of metoclopramide (experimental group - E) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (E3 and C3) or seventh postoperative day (E7 and C7). Adhesion was assessed, and a colonic segment containing the anastomosis was removed for analysis of breaking strength and hydroxyproline concentration. RESULTS: There were no deaths or dehiscence on the 3rd postoperative day. There was one death and one blocked anastomotic dehiscence in the E7 group. No significant differences between groups were found in the analysis of clinical outcome, intra-cavity adhesion, adhesion to the anastomosis or breaking strength on the 3rd and 7th postoperative day. Hydroxyproline concentration was higher in the control group on the 3rd (p=0.006) but not on the 7th postoperative day (p=0.241). CONCLUSION: Metoclopramide did not have harmful effects on the healing of intestinal anastomoses in rats.

scholarly journals Effects of abdominal sepsis in the healing of abdominal wall: experimental study in rats

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 38-44 ◽  
Author(s):  
Alexandre Malta Brandão ◽  
Naiara Galvão da Silva ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Breaking Strength ◽  
Abdominal Sepsis ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Postoperative Sepsis ◽  
Silk Thread ◽  
The Third ◽  
The Mean

PURPOSE: To evaluate the influence of sepsis in the process of wound healing in the abdominal wall. METHODS: 40 rats divided into two groups of twenty animals: group of study (E) - septic, and the control group (C) - not septic. The two groups were divided into subgroups of 10 to be killed on the third day (n = 10) or seventh (n = 10) postoperative. Sepsis was induced by ligation and puncture of the cecum. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. On the day of re-laparotomy, the abdominal wall was removed for analysis of the breaking strength and histopathological analysis. RESULTS: The mean breaking strength was at third day: E group (1.44 ± 1.22) and C group (0.35 ± 0.46). At seventh day the mean breaking strength was: E group (13.01 ± 7.09) and C group (11.66 ± 7.38). There was statistical difference in tensile strength. E group at third day with control group (p = 0.019). CONCLUSION: The induction of peritoneal sepsis reduced the breaking strength of the abdominal wall on the third day after surgery.

scholarly journals Influence of metoclopramide on abdominal wall healing in rats subjected to colonic anastomosis in the presence of peritoneal sepsis induced

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 92-99 ◽  
Author(s):  
Naiara Galvão da Silva ◽  
Alexandre Malta Brandão ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Foreign Body Reaction ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Cecal Ligation ◽  
Left Colon ◽  
Control Group ◽  
Silk Thread ◽  
Inflammatory Infiltration ◽  
Histopathological Evaluation

PURPOSE: To evaluate the effects of metoclopramide on abdominal wall healing in rats in the presence of sepsis. METHODS: 40 rats divided into two groups of twenty animals, subdivided into two subgroups of 10 animals each: group (E) - treated with metoclopramide, and saline-treated control group. The two groups were divided into subgroups of 10 to be killed on the 3rd day (n = 10) or day 7 (n = 10) after surgery. Sepsis was induced by cecal ligation and puncture. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. We measured the breaking strength of the abdominal wall and made the histopathological evaluation. RESULTS: on 3rd day postoperative, the average breaking strength in the E group was 0.83 ± 0.66 and in group C was 0.35 ± 0.46 (p = 0.010). On the seventh day, the breaking strength in group E was11.44 ± 5.07, in group C 11.66 ± 7.38 (p = 1.000). The E7 group showed lower inflammatory infiltration, foreign body reaction, fibrin than control. CONCLUSION: animals treated with metoclopramide had a higher resistance of the abdominal wall on the 3rd postoperative day.

scholarly journals Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

2012 ◽  
Vol 27 (12) ◽  
pp. 892-896 ◽  
Author(s):  
Romulo Medeiros de Almeida ◽  
João Batista de Sousa ◽  
Paulo Roberto Faria Ribeiro ◽  
Silvana Marques e Silva ◽  
Marco Aurélio Pereira Firmino ◽  
...  
Keyword(s):  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Control Group ◽  
Nitric Oxide Synthase Inhibitor ◽  
The Third ◽  
Significant Difference ◽  
Nitrite Nitrate ◽  
Synthase Inhibitor ◽  
Oxide Synthase ◽  
And Control

PURPOSE: To evaluate the effects of S-methylisothiourea hemisulfate (SMT) on the healing of colonic anastomosis in rats. METHODS: Sixty rats Wistar were distributed into two groups of 30 animals: experimental (E) and control C). The animals of experimental group received intraperitoneal SMT at 50mg/kg/dose every 12 hours for 72 hours. The control group received intraperitoneal saline at the same volume of SMT. The rats were subdivided into subgroups groups of 10 for euthanasia on the third, seventh, and 14th postoperative days (POD). We evaluated clinical and weight evolution, breaking strength and histopathology; also, a blood sample was collected for serum dosage of nitrite/nitrate. RESULTS: There was more vascular neoformation (p=0.006) and granulation (p=0.002) in the E3 group, and more mononuclear infiltrates in the C3 group (p=0.041). There was also more edema in the C14 group (p=0.008). There was no statistically significant difference in breaking strength, nitrite/nitrate dosage, and the remaining histopathological parameters. CONCLUSION: The use of S-methylisothiourea hemisulfate improved the healing of colonic anastomosis in rats on the third postoperative day by accelerating the proliferative stage of healing, but without interfering with the breaking strength of the anastomosis.

scholarly journals DOES THE ASSOCIATION OF TACROLIMUS AND MYCOPHENOLATE MOFETIL CHANGE THE HEALING OF THE ABDOMINAL WALL? STUDY IN RATS SUBMITTED TO ISCHEMIA AND KIDNEY REPERFUSION

2020 ◽  
Vol 33 (4) ◽  
Author(s):  
André Luís Conde WATANABE ◽  
Jorge Eduardo Fouto MATIAS
Keyword(s):  
Mycophenolate Mofetil ◽  
Abdominal Wall ◽  
Histological Examination ◽  
Postoperative Period ◽  
Breaking Strength ◽  
Immunosuppressive Agents ◽  
Control Group ◽  
Strength Measurement ◽  
Standard Operation ◽  
Group I

ABSTRACT Background: Tacrolimus and mycophenolate mofetil are immunosuppressive agents widely used on the postoperative period of the transplants. Aim: To evaluate the influence of the association of them on the abdominal wall healing in rats. Methods: Thirty-six Wistar rats were randomly assigned in three groups of 12. On the early postoperative period, four of the control group and three of the experimental groups died. The three groups were nominated as follow: control group (GC, n=8); group I (GI, n=11, standard operation, mycophenolate mofetil and tacrolimus); group II (GII, n=10, standard operation, mycophenolate mofetil and tacrolimus). The standard operation consisted of right total nephrectomy and 20 min ischemia of the left kidney followed by reperfusion. Both NaCl 0.9% and the immunosuppressive agents were administered starting on the first postoperative day and continuing daily until the day of death on the 14th day. On the day of their deaths, two strips of the anterior abdominal wall were collected and submitted to breaking strength measurement and histological examination. Results: There were no significant differences in wound infection rates (p=0,175), in the breaking strength measurement and in the histological examination among the three groups. Conclusion: The combination of the immunosuppressive agents used in the study associated with renal ischemia and reperfusion does not interfere in the abdominal wall healing of rats.

scholarly journals Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon

2012 ◽  
Vol 27 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Pedro Henrique Alves de Morais ◽  
Igor Eduardo Caetano de Farias ◽  
Leonardo de Castro Durães ◽  
Fabiana Pirani Carneiro ◽  
Paulo Gonçalves de Oliveira ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Wound Healing ◽  
Tensile Strength ◽  
Abdominal Wall ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Abdominal Closure ◽  
Carbon Dioxide Pneumoperitoneum ◽  
Resection And Anastomosis

PURPOSE: To evaluate the influence of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats. METHODS: Eighty rats underwent laparotomy, segmental left colon resection, and anastomosis. The animals were divided into three experimental groups and one control group: EI = pneumoperitoneum for 30 minutes before laparotomy (n=20); EII = pneumoperitoneum for 30 minutes after abdominal closure (n=20); EIII = pneumoperitoneum for 30 minutes before laparotomy and 30 minutes after abdominal closure (n=20); C = control group, without pneumoperitoneum (n=20). In each group, 10 animals were killed 7 days and 10 animals 14 days postoperatively. A segment of the abdominal wall was resected and subjected to tensile strength testing. Another segment of abdominal muscle was used for histopathological analysis; the specimens were fixed in formalin and stained with hematoxylin and eosin. RESULTS: There were no differences in histopathology and tensile strength values among animals in the experimental and control groups 7 or 14 days after surgery. CONCLUSION: Under the present experimental conditions, carbon dioxide pneumoperitoneum did not interfere with abdominal wall wound healing.

scholarly journals Fatal and non-fatal inflammatory injuries induced by different concentrations of L-arginine in Kunming rats

2018 ◽  
Vol 16 ◽  
pp. 1721727X1775381
Author(s):  
Chunxia Li ◽  
Ge Zhang ◽  
Aijun Dai ◽  
Xiuyan Wang
Keyword(s):  
Mortality Rate ◽  
Animal Models ◽  
Control Group ◽  
Pathological Changes ◽  
The Third ◽  
Group A ◽  
Inflammatory Edema ◽  
Group B ◽  
Biochemical Indices

To establish animal models of fatal and non-fatal pancreatitis, we randomly divided 55 Kunming rats into three groups: Group A received three intraperitoneal injections of 8% L-arginine (L-arg); Group B received three intraperitoneal injections of 10% L-arg; and Group C (the control group) received three intraperitoneal injections of saline. After the third injection, biochemical indices and pathological changes in the pancreas and lungs were evaluated. In Group A, the rats experienced peak inflammatory edema at 48 h after the injections and exhibited the highest pathological score, but the biochemical indices showed no phase-related changes. In Group B, the rats exhibited significantly increased biochemical indices at 12 h after injection and experienced peak inflammatory edema at 24 h; the pathological score was also the highest, significantly different from the observations for Groups A and C ( P < 0.05), and the mortality rate was 64% over 72 h. Thus, it was inferred that L-arg could be used to establish pancreatitis models and that different concentrations lead to different lesions.

scholarly journals Effects of the povidone-iodine (PVPI) in treatment of bacterial peritonitis induced in rats

2010 ◽  
Vol 25 (4) ◽  
pp. 322-327 ◽  
Author(s):  
Ivana Duval de Araujo ◽  
Giovanni Cezar Xavier Grossi ◽  
Simone Odília Fernandes Diniz ◽  
Tarcizo Afonso Nunes ◽  
Eduardo Ângelo Braga ◽  
...  
Keyword(s):  
Peritoneal Cavity ◽  
Saline Solution ◽  
Povidone Iodine ◽  
Absorption Index ◽  
Control Group ◽  
Phagocytic Cells ◽  
Bacterial Peritonitis ◽  
Viable Bacteria ◽  
Group A ◽  
Group B

PURPOSE: To evaluate the effectiveness of the use of the povidone-iodine (PVI) added to the liquid of wash of the peritoneal cavity in the reduction of bacterial absorption and in the remainder non-phagocyted bacteria in the circulating blood of rat. METHODS: Thirty four Wistar females rats were used, distributed in the following groups: A (n=10), non-treated; B (n=9), wash of the peritoneal cavity with solution of PVI to 1% in saline solution; C (n=15), wash of the cavity with saline solution. After anesthesia, it was made intraperitoneal infusion of solution of Escherichia coli labeled with 99mTc containing 10(8) CFU/ml. After 40 minutes, it was made the treatment, in the group A, manipulation of the viscera; in the group B, irrigation of the peritoneal cavity with warm solution of 1% PVPI to 37,5ºC, and in the group C irrigation with warm saline (37,5ºC). After 15 minutes of the treatment, blood samples and fragments of liver, spleen and lung was obtained for count of the radioactivity, and animals killed by abdominal aorta section. There were determined the bacterial absorption index and the remainder index in the bloodstream. RESULTS: Of the total of bacteria infused in the peritoneum, there was absorption of 0,92% (0,14% to 2,13%) in the animals of the group A (controls), 0,49% (0,18% to 0,71%) after use of topical PVPI (group B) and 0,80% (0,04% to 3,8%) after wash with saline solution (group C). There was significant reduction of the absorption when compared the treated animals with PVPI and the controls (p=0,003). Of the total of bacteria absorbed for the circulatory current, the percentile amount of bacteria non-phagocyted in the outlying blood was of 2,9% (1,1% to 17,7%) in the control group, 15,2% (8,3% to 21,4%) in those treated with PVPI (group B) and 6,9% (0,8% to 29,7%) after wash with saline solution (group C), with difference among controls and treated with PVPI (p=0,01). CONCLUSION: The wash of the cavity peritoneal of mice with solution containing PVPI showed to be capable to reduce the absorption of bacteria by peritoneum of rat; however it seems to interfere with the function of the phagocytic cells for the observation of the increase of viable bacteria in the outlying blood of those animals.

scholarly journals Antimicrobial prophylaxis in addition to premedication in gastrointestinal surgery

2014 ◽  
Vol 64 (1) ◽  
pp. 149-155
Author(s):  
Alan Genter ◽  
Hrvoje Capak ◽  
Marija Lipar ◽  
Marko Samardžija ◽  
Ivica Harapin ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Clavulanic Acid ◽  
Enterococcus Faecalis ◽  
Saline Solution ◽  
Antimicrobial Prophylaxis ◽  
Gastrointestinal Surgery ◽  
Control Group ◽  
Sterile Syringe ◽  
E Coli ◽  
Number Of Bacteria

Abstract The aim of this study was to evaluate three combinations of antibiotics applied perorally prior to laparotomy and gastrointestinal surgery and to select the most favorable combination. Research was performed on 24 female and male dogs divided into four groups. Following abdominal wall incision the caecum was compressed manually and 5 mL of saline solution was injected into it. Five mL of caecum contents was aspirated in a sterile syringe and only 0.1 mL was incubated into blood agar and homogenous agar. Bacterial colonies were counted and determined. Prior to surgery the control group (n=6) received no antibiotics. The group which received the combination of gentamicin and clindamycin (n=6) achieved the best reduction of E. coli whereas Enterococcus faecals was 100% destroyed. The group which received amoxicillin with clavulanic acid and metronidazole (n=6) acquired a reduction of bacteria Enterococcus faecalis, which could be sufficient for prophylaxis. The combination of gentamicin and erythromycin (n=6) failed to accomplish a reduction of the number of bacteria.

scholarly journals Use of the amniotic membrane to cover the peritoneal cavity in the reconstruction of the abdominal wall with polypropylene mesh in rats

2015 ◽  
Vol 42 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Rafael Calvão Barbuto ◽  
Ivana Duval de Araujo ◽  
Daniel de Oliveira Bonomi ◽  
Luciene Simões de Assis Tafuri ◽  
Antônio Calvão Neto ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Amniotic Membrane ◽  
Polypropylene Mesh ◽  
Control Group ◽  
Angiogenic Activity ◽  
Microscopic Evaluation ◽  
Significant Difference ◽  
Female Wistar Rats ◽  
Group A ◽  
Group B

OBJECTIVE: to evaluate the efficacy of the amniotic membrane used with polypropylene mesh against the formation of adhesions and its influence on healing. METHODS: twenty five female Wistar rats were anesthetized for creating a parietal defect in the anterior abdominal wall. Its correction was made with polypropylene mesh alone and associated with amniotic membrane. In the control group (n=11), the screen was inserted alone. In group A (n=7) we interposed the amniotic membrane between the screen and the abdominal wall. In group B, the amniotic membrane was placed on the mesh, covering it. After seven days, the animals were euthanized for macroscopic and microscopic evaluation of healing. RESULTS: adhesions were observed in all animals except one in the control group. Severe inflammation was observed in all animals in groups A and B and in three of the control group, with significant difference between them (A and B with p=0.01). Pronounced angiogenic activity was noted in one animal in the control group, six in group A and four in group B, with a significant difference between the control group and group A (p=0.002) and group B (p=0.05). The scar collagen was predominantly mature, except in five animals of the control group, with significant difference between the control group and group A (p=0.05) and group B (p=0.05). CONCLUSION: The amniotic membrane did not alter the formation of adhesions in the first postoperative week. There were also pronounced inflammation, high angiogenic activity and predominance of mature collagen fibers, regardless of the anatomical plane that it was inserted in.

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