scholarly journals Influence of metoclopramide on abdominal wall healing in rats subjected to colonic anastomosis in the presence of peritoneal sepsis induced

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 92-99 ◽  
Author(s):  
Naiara Galvão da Silva ◽  
Alexandre Malta Brandão ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Foreign Body Reaction ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Cecal Ligation ◽  
Left Colon ◽  
Control Group ◽  
Silk Thread ◽  
Inflammatory Infiltration ◽  
Histopathological Evaluation

PURPOSE: To evaluate the effects of metoclopramide on abdominal wall healing in rats in the presence of sepsis. METHODS: 40 rats divided into two groups of twenty animals, subdivided into two subgroups of 10 animals each: group (E) - treated with metoclopramide, and saline-treated control group. The two groups were divided into subgroups of 10 to be killed on the 3rd day (n = 10) or day 7 (n = 10) after surgery. Sepsis was induced by cecal ligation and puncture. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. We measured the breaking strength of the abdominal wall and made the histopathological evaluation. RESULTS: on 3rd day postoperative, the average breaking strength in the E group was 0.83 ± 0.66 and in group C was 0.35 ± 0.46 (p = 0.010). On the seventh day, the breaking strength in group E was11.44 ± 5.07, in group C 11.66 ± 7.38 (p = 1.000). The E7 group showed lower inflammatory infiltration, foreign body reaction, fibrin than control. CONCLUSION: animals treated with metoclopramide had a higher resistance of the abdominal wall on the 3rd postoperative day.

scholarly journals Effects of abdominal sepsis in the healing of abdominal wall: experimental study in rats

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 38-44 ◽  
Author(s):  
Alexandre Malta Brandão ◽  
Naiara Galvão da Silva ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Breaking Strength ◽  
Abdominal Sepsis ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Postoperative Sepsis ◽  
Silk Thread ◽  
The Third ◽  
The Mean

PURPOSE: To evaluate the influence of sepsis in the process of wound healing in the abdominal wall. METHODS: 40 rats divided into two groups of twenty animals: group of study (E) - septic, and the control group (C) - not septic. The two groups were divided into subgroups of 10 to be killed on the third day (n = 10) or seventh (n = 10) postoperative. Sepsis was induced by ligation and puncture of the cecum. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. On the day of re-laparotomy, the abdominal wall was removed for analysis of the breaking strength and histopathological analysis. RESULTS: The mean breaking strength was at third day: E group (1.44 ± 1.22) and C group (0.35 ± 0.46). At seventh day the mean breaking strength was: E group (13.01 ± 7.09) and C group (11.66 ± 7.38). There was statistical difference in tensile strength. E group at third day with control group (p = 0.019). CONCLUSION: The induction of peritoneal sepsis reduced the breaking strength of the abdominal wall on the third day after surgery.

scholarly journals Effects of bromopride on abdominal wall healing with induced peritoneal sepsis after segmental colectomy and colonic anastomosis in rats

2011 ◽  
Vol 26 (6) ◽  
pp. 433-437 ◽  
Author(s):  
Marcos Vinícius Melo de Oliveira ◽  
Alexandre Malta Brandão ◽  
Pedro Henrique Alves de Morais ◽  
Naiara Galvão da Silva ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Saline Solution ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Left Colon ◽  
Control Group ◽  
The Third ◽  
Segmental Colectomy ◽  
Group B ◽  
Harmful Effects

PURPOSE: Evaluate the effects of bromopride on abdominal wall healing of rats with induced peritoneal sepsis after segmental colectomy and colonic anastomosis. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of bromopride (bromopride group - B) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (GB3 and GC3) or seventh postoperative day (GB7 and GC7). It was analyzed the following characteristics: breaking strength of the abdominal wall's wound; surgical and histopathological features of the abdominal wall; and clinical features of the rats. RESULTS: There was no difference between the groups in relation to the weight of the rats and the breaking strength of the abdominal wall's wound. The GB7 group presented less edema and less quantity of fibrin during histopathological evaluation compared to the GC7 group. CONCLUSION: Bromopride did not have harmful effects on the healing of abdominal wall in rats.

scholarly journals DOES THE ASSOCIATION OF TACROLIMUS AND MYCOPHENOLATE MOFETIL CHANGE THE HEALING OF THE ABDOMINAL WALL? STUDY IN RATS SUBMITTED TO ISCHEMIA AND KIDNEY REPERFUSION

2020 ◽  
Vol 33 (4) ◽  
Author(s):  
André Luís Conde WATANABE ◽  
Jorge Eduardo Fouto MATIAS
Keyword(s):  
Mycophenolate Mofetil ◽  
Abdominal Wall ◽  
Histological Examination ◽  
Postoperative Period ◽  
Breaking Strength ◽  
Immunosuppressive Agents ◽  
Control Group ◽  
Strength Measurement ◽  
Standard Operation ◽  
Group I

ABSTRACT Background: Tacrolimus and mycophenolate mofetil are immunosuppressive agents widely used on the postoperative period of the transplants. Aim: To evaluate the influence of the association of them on the abdominal wall healing in rats. Methods: Thirty-six Wistar rats were randomly assigned in three groups of 12. On the early postoperative period, four of the control group and three of the experimental groups died. The three groups were nominated as follow: control group (GC, n=8); group I (GI, n=11, standard operation, mycophenolate mofetil and tacrolimus); group II (GII, n=10, standard operation, mycophenolate mofetil and tacrolimus). The standard operation consisted of right total nephrectomy and 20 min ischemia of the left kidney followed by reperfusion. Both NaCl 0.9% and the immunosuppressive agents were administered starting on the first postoperative day and continuing daily until the day of death on the 14th day. On the day of their deaths, two strips of the anterior abdominal wall were collected and submitted to breaking strength measurement and histological examination. Results: There were no significant differences in wound infection rates (p=0,175), in the breaking strength measurement and in the histological examination among the three groups. Conclusion: The combination of the immunosuppressive agents used in the study associated with renal ischemia and reperfusion does not interfere in the abdominal wall healing of rats.

scholarly journals Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon

2012 ◽  
Vol 27 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Pedro Henrique Alves de Morais ◽  
Igor Eduardo Caetano de Farias ◽  
Leonardo de Castro Durães ◽  
Fabiana Pirani Carneiro ◽  
Paulo Gonçalves de Oliveira ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Wound Healing ◽  
Tensile Strength ◽  
Abdominal Wall ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Abdominal Closure ◽  
Carbon Dioxide Pneumoperitoneum ◽  
Resection And Anastomosis

PURPOSE: To evaluate the influence of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats. METHODS: Eighty rats underwent laparotomy, segmental left colon resection, and anastomosis. The animals were divided into three experimental groups and one control group: EI = pneumoperitoneum for 30 minutes before laparotomy (n=20); EII = pneumoperitoneum for 30 minutes after abdominal closure (n=20); EIII = pneumoperitoneum for 30 minutes before laparotomy and 30 minutes after abdominal closure (n=20); C = control group, without pneumoperitoneum (n=20). In each group, 10 animals were killed 7 days and 10 animals 14 days postoperatively. A segment of the abdominal wall was resected and subjected to tensile strength testing. Another segment of abdominal muscle was used for histopathological analysis; the specimens were fixed in formalin and stained with hematoxylin and eosin. RESULTS: There were no differences in histopathology and tensile strength values among animals in the experimental and control groups 7 or 14 days after surgery. CONCLUSION: Under the present experimental conditions, carbon dioxide pneumoperitoneum did not interfere with abdominal wall wound healing.

scholarly journals Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

2012 ◽  
Vol 27 (12) ◽  
pp. 892-896 ◽  
Author(s):  
Romulo Medeiros de Almeida ◽  
João Batista de Sousa ◽  
Paulo Roberto Faria Ribeiro ◽  
Silvana Marques e Silva ◽  
Marco Aurélio Pereira Firmino ◽  
...  
Keyword(s):  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Control Group ◽  
Nitric Oxide Synthase Inhibitor ◽  
The Third ◽  
Significant Difference ◽  
Nitrite Nitrate ◽  
Synthase Inhibitor ◽  
Oxide Synthase ◽  
And Control

PURPOSE: To evaluate the effects of S-methylisothiourea hemisulfate (SMT) on the healing of colonic anastomosis in rats. METHODS: Sixty rats Wistar were distributed into two groups of 30 animals: experimental (E) and control C). The animals of experimental group received intraperitoneal SMT at 50mg/kg/dose every 12 hours for 72 hours. The control group received intraperitoneal saline at the same volume of SMT. The rats were subdivided into subgroups groups of 10 for euthanasia on the third, seventh, and 14th postoperative days (POD). We evaluated clinical and weight evolution, breaking strength and histopathology; also, a blood sample was collected for serum dosage of nitrite/nitrate. RESULTS: There was more vascular neoformation (p=0.006) and granulation (p=0.002) in the E3 group, and more mononuclear infiltrates in the C3 group (p=0.041). There was also more edema in the C14 group (p=0.008). There was no statistically significant difference in breaking strength, nitrite/nitrate dosage, and the remaining histopathological parameters. CONCLUSION: The use of S-methylisothiourea hemisulfate improved the healing of colonic anastomosis in rats on the third postoperative day by accelerating the proliferative stage of healing, but without interfering with the breaking strength of the anastomosis.

scholarly journals Does metoclopramide impair anastomotic healing of the left colon of rats?

2011 ◽  
Vol 26 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Silvana Marques e Silva ◽  
Vânia Maria Moraes Ferreira ◽  
Omar Feres ◽  
Paulo Gonçalves de Oliveira ◽  
João Batista de Sousa
Keyword(s):  
Saline Solution ◽  
Breaking Strength ◽  
Anastomotic Dehiscence ◽  
Left Colon ◽  
Control Group ◽  
Colonic Anastomoses ◽  
Intestinal Anastomoses ◽  
Hydroxyproline Concentration ◽  
Harmful Effects ◽  
Experimental Group

PURPOSE: To evaluate the effects of metoclopramide on the formation of adhesion and the healing of left colonic anastomoses in rats. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of metoclopramide (experimental group - E) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (E3 and C3) or seventh postoperative day (E7 and C7). Adhesion was assessed, and a colonic segment containing the anastomosis was removed for analysis of breaking strength and hydroxyproline concentration. RESULTS: There were no deaths or dehiscence on the 3rd postoperative day. There was one death and one blocked anastomotic dehiscence in the E7 group. No significant differences between groups were found in the analysis of clinical outcome, intra-cavity adhesion, adhesion to the anastomosis or breaking strength on the 3rd and 7th postoperative day. Hydroxyproline concentration was higher in the control group on the 3rd (p=0.006) but not on the 7th postoperative day (p=0.241). CONCLUSION: Metoclopramide did not have harmful effects on the healing of intestinal anastomoses in rats.

scholarly journals Effects of enoxaparin on the healing of abdominal wall in rats: study on breaking strength and histopatology

2006 ◽  
Vol 21 (4) ◽  
pp. 247-251 ◽  
Author(s):  
Paulo Gonçalves de Oliveira ◽  
João Batista de Sousa ◽  
Luiz Guilherme C. V. Reys ◽  
Albino Verçosa de Magalhães ◽  
Francieli de Sousa Rabelo ◽  
...  
Keyword(s):  
Body Weight ◽  
Abdominal Wall ◽  
Breaking Strength ◽  
Anterior Abdominal Wall ◽  
Male Rats ◽  
Histopathological Study ◽  
Significant Difference ◽  
Histopathological Evaluation ◽  
Median Laparotomy ◽  
Wistar Male Rats

PURPOSE: To evaluate the influence of enoxaparin on the abdominal wall healing. METHODS: Sixty Wistar male rats were randomly distributed into two experimental groups. Group E: consisted of 30 rats, submitted to abdominal suture and treated with enoxaparin in a dose of 1 mg/kg of body weight, subcutaneously administered from immediate postoperative until the 7th day; Group C: consisted of 30 rats submitted to abdominal suture and treated with the equivalent dose per volume of NaCl 0.9%. The animals were submitted to median laparotomy and were operated again at the 3rd, 7th or 14th postoperative day for the resection of the anterior abdominal wall. The tissue's stretching resistance was tested and a histopathological evaluation was performed. Through Mann-Whitney's U test, a statistical analysis was conducted considering significant values for p <0.05. RESULTS: The tensile breaking strength presented a median of 0.64N for group C and 0.41N for group E (p=0.60) at the 3rd postoperative day; of 2.27N for group C and of 4.32N for group E (p=0.01) at the 7th POD; of 5.72N for group C and of 6.62N for group E (p=0.97) at the 14th POD. In relation to the histopathological study, the results did not show significant statistical differences, except for the amount of collagen at the 7th POD, which medians were 2 on group C and 1 on group E (p=0.007). CONCLUSION: Considering the conditions in which this study was conducted, the administration of enoxaparin led to an strengthening of the tissue's breaking resistance at the 7th POD; on the histopathological exam, a significant difference was observed at the 7th POD, with a better collagen disposition on group C.

scholarly journals Polypropylene meshes coated with chitosan/polyethylene glycol for the reconstruction of the abdominal wall: an experimental study in rats

2019 ◽  
Vol 71 (4) ◽  
pp. 1198-1206
Author(s):  
D.F. Rodrigues ◽  
F.F. Mendes ◽  
R.M. Melo ◽  
L.B. Menezes ◽  
L.L.B. Guimarães ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Foreign Body ◽  
Abdominal Wall ◽  
Foreign Body Reaction ◽  
Tissue Reaction ◽  
Collagen Fibers ◽  
Control Group ◽  
Histochemical Analysis ◽  
Peritoneal Adhesions ◽  
Tissue Reactions

ABSTRACT The aim of this study was to characterize the tissue reactions triggered by the polypropylene mesh coated with chitosan and polyethylene glycol film, and if it’s able to prevent the formation of peritoneal adhesions. Defects in the abdominal wall of rats were induced and polypropylene meshes coated with chitosan/polyethylene glycol (CPEG group, n= 12) and uncoated (PP control group, n= 12) were implanted. On the fourth and forty-fifth postoperative day the formation of adhesion and the tissue reaction to the biomaterial was evaluated through histological and histochemical analysis. The area (P= 0.01) and severity (P= 0.002) of the adhesion was significatively less in the CPEG group. On the fourth day the foreign body reaction was less intense in CPEG group (P= 0.018) and the production of collagen fibers was more intense in this group (P= 0.041). The tissue reactions caused by the biomaterials were similar on the 45th day, with the exception of the high organization of collagen fibers in the CPEG group. The CPEG meshes did not fully prevent the formation of adhesions, but minimized the severity of the process. The foreign body reaction promoted by polypropylene meshes coated with CPEG is less intense than that triggered by uncoated polypropylene meshes.

Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

2020 ◽  
Vol 28 (3) ◽  
pp. 323-333
Author(s):  
Sergej V. Ivanov ◽  
Ilya S. Ivanov ◽  
Evgenij G. Obyedkov ◽  
Liliya P. Popova
Keyword(s):  
Abdominal Wall ◽  
Inflammatory Reaction ◽  
Inflammatory Process ◽  
Anterior Abdominal Wall ◽  
Dynamic Change ◽  
Main Group ◽  
Control Group ◽  
Surgical Department ◽  
Regeneration Stage

Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

scholarly journals Comparison of the Abdominal Wall Muscle Thickness in Female Rugby Players Versus Non-Athletic Women: A Cross-Sectional Study

Medicina ◽  
2019 ◽  
Vol 56 (1) ◽  
pp. 8 ◽  
Author(s):  
Vanesa Abuín-Porras ◽  
Mónica de la Cueva-Reguera ◽  
Pedro Benavides-Morales ◽  
Rocío Ávila-Pérez ◽  
Blanca de la Cruz-Torres ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Female Athletes ◽  
Muscular Activity ◽  
Transversus Abdominis ◽  
Control Group ◽  
Neck Muscle ◽  
Cross Sectional ◽  
Sport Practice ◽  
Ultrasound Evaluation ◽  
Rugby Players

Background and Objectives: Rugby players engage in demanding, high loading muscular activity in the spine. Study of the abdominal wall architecture in female rugby athletes is relevant to the possible muscular asymmetry secondary to sport practice and the relationship between the abdominal wall and the pelvic floor muscles. Activation of the transversus abdominis (TrAb) generates an increase in the bladder neck muscle. Moreover, an increased interrecti distance (IRD) is related to urinary incontinence and has a higher prevalence in athletic women. The aim of the present study was to compare and quantify, with ultrasound imaging (USI), the thickness of the transversus abdominis (TrAb), external oblique (EO), internal oblique (IO), rectus abdominis (RA), and interrecti distance (IRD) in female rugby players versus non-athletic women in order to improve upon existing knowledge about abdominal wall configuration in female athletes. Materials and Methods: A sample of 32 women was recruited at the Universidad Europea Research Lab and divided in two groups: a rugby group (n = 16) and a non-athletic women group (n = 16). The thickness of the TrAb, EO, IO, RA, and IRD were assessed by USI in both groups. Results: There were statistically significant differences for the ultrasound evaluation thickness of the right TrAb (p = 0.011; d = 0.10), EO (p = 0.045; d = 0.74), IO (p = 0.003; d = 1.32), and RA (p = 0.001; d = 1.38) showing a thickness increase for the rugby group with respect to the control group. For the IRD thickness, there were no significant differences (p > 0.05) between groups. Conclusions: An increased TrAb, IO, EO, and RA thickness may be shown in female rugby players versus non-athletic women. Nevertheless, statistically relevant differences were not found for the IRD between both groups.

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