scholarly journals DOES THE ASSOCIATION OF TACROLIMUS AND MYCOPHENOLATE MOFETIL CHANGE THE HEALING OF THE ABDOMINAL WALL? STUDY IN RATS SUBMITTED TO ISCHEMIA AND KIDNEY REPERFUSION

2020 ◽  
Vol 33 (4) ◽  
Author(s):  
André Luís Conde WATANABE ◽  
Jorge Eduardo Fouto MATIAS
Keyword(s):  
Mycophenolate Mofetil ◽  
Abdominal Wall ◽  
Histological Examination ◽  
Postoperative Period ◽  
Breaking Strength ◽  
Immunosuppressive Agents ◽  
Control Group ◽  
Strength Measurement ◽  
Standard Operation ◽  
Group I

ABSTRACT Background: Tacrolimus and mycophenolate mofetil are immunosuppressive agents widely used on the postoperative period of the transplants. Aim: To evaluate the influence of the association of them on the abdominal wall healing in rats. Methods: Thirty-six Wistar rats were randomly assigned in three groups of 12. On the early postoperative period, four of the control group and three of the experimental groups died. The three groups were nominated as follow: control group (GC, n=8); group I (GI, n=11, standard operation, mycophenolate mofetil and tacrolimus); group II (GII, n=10, standard operation, mycophenolate mofetil and tacrolimus). The standard operation consisted of right total nephrectomy and 20 min ischemia of the left kidney followed by reperfusion. Both NaCl 0.9% and the immunosuppressive agents were administered starting on the first postoperative day and continuing daily until the day of death on the 14th day. On the day of their deaths, two strips of the anterior abdominal wall were collected and submitted to breaking strength measurement and histological examination. Results: There were no significant differences in wound infection rates (p=0,175), in the breaking strength measurement and in the histological examination among the three groups. Conclusion: The combination of the immunosuppressive agents used in the study associated with renal ischemia and reperfusion does not interfere in the abdominal wall healing of rats.

scholarly journals CHANGE IN THE QUALITY OF LIFE OF CHILDREN AFTER SURGICAL TREATMENT OF RETINAL DETACHMENT

2018 ◽  
Vol 25 (6) ◽  
pp. 149-153
Author(s):  
A. N. Sergienko ◽  
V. V. Dashina ◽  
A. V. Malyshev ◽  
O. I. Lysenko ◽  
S. V. Yanchenko
Keyword(s):  
Quality Of Life ◽  
Statistical Analysis ◽  
Surgical Treatment ◽  
Retinal Detachment ◽  
Postoperative Period ◽  
Control Group ◽  
Russian Version ◽  
Group I ◽  
Group Ii

Aim.The study was designed for the evaluation of the quality of life of children with retinal detachment during vitrectomy.Materials and methods. 23 children with retinal detachment aged from 10 to 17 years were treated. Surgical treatment consisted of a three-port vitrectomy and a laser endocoagulation of the retina. 3 groups were formed with the help of a random sampling. During the surgical treatment of group I (n=8), only balanced non-oxidant solutions of salts (Balsed Salt Solution − BSS) were used. Tablets of antioxidant preparations were not assigned. Saline solutions with antioxidants (BSS plus) were used to carry out surgery for group II (n=8). Group III (n=7) was additionally taking antioxidant preparations peros for 3 months in the postoperative period . The quality of life was studied using the abridged Russian version of the VFQ-25 questionnaire. 10 children of the same age with no indications of surgical treatment of retinal detachment were selected for the control group. Statistical analysis of the obtained data was carried out using a set of programs for applied statistical analysis Analyst Soft, Bio Stat 2007.Results.A week after the surgical treatment, no increase in visometric data was observed. Six months later, a significant increase in visual acuity was revealed. The highest visometric data were observed in group II due to the minimal progression of lens opacities. Group I patients had the lowest values of this index.Conclusion.The positive effect of the surgical treatment of retinal detachment positively influenced the patients’ quality of life in the early and late postoperative period.

scholarly journals Histological changes of the arterial bed of the hind limbs of the rats under condition of the acute ischemia-reperfusion and correction with the carbacetam

2020 ◽  
Vol 26 (2) ◽  
pp. 5-11
Author(s):  
T.O. Veresiuk ◽  
P.R. Selskyy ◽  
A.T. Televiak
Keyword(s):  
Histological Examination ◽  
Ischemia Reperfusion ◽  
Control Group ◽  
Acute Ischemia ◽  
Histological Changes ◽  
Arterial Blood ◽  
Vascular Bed ◽  
Hind Limbs ◽  
Group I ◽  
Ischemic Period

The ischemic-reperfusion lesion is a complex multifactorial damage of the primary ischemic tissues as a result of restoration of the arterial blood circulation in them, which is accompanied by local morpho-functional reorganization of the vascular bed of the hind limbs of the rats. One of the promising means in the treatment and prevention of the reperfusion disorders is a carbacetam, which smooths the phenomena of hypo- and hyperperfusion in the post-ischemic period. The aim of the study was to established the manifestations of the morpho-functional remodeling of the vascular bed of the hind limbs of the rats in ischemia-reperfusion and under conditions of correction with carbacetam. Histological examination of the vascular bed of the hind limbs of 30 rats under conditions of ischemia-reperfusion (group I) and 30 rats in the simulation of ischemia-reperfusion, which in the post-ischemic period administered carbacetam once a day (5 mg/kg) for 14 days (group II) were done. There were 6 intact animals in the control group. Simulation of ischemia was performed by applying SWAT rubber tourniquets on the hind limbs for 2 hours, and reperfusion – by removing of the tourniquet. The animals of the experimental groups were divided into 5 subgroups with reperfusion terms after 1, 2 hours and 1 day, as well as after 7 and 14 days. Histological examination was performed according to generally accepted methods. The vascular bed in the middle third of the thigh and the shin below the tourniquet was examined using a Bresser Trino Researcher 40x–1000x microscope. Analyzing of the obtained results, was established that after 1 hour of the reperfusion the histological changes became a systemic, and after 1 day it were more significant. It should be noted that the thickness of the vessel walls increased, and the elastic membranes were partially eligned, thinned and torned. The stepwise clarity of the arterials walls structure was lost. The edema acquired a total nature. The histological examination of the vessels after 7 days revealed that the swelling of the walls decreased and the condition of the elastic frame was improved. There was a proliferation of collagen fibers in the adventitia, which was a response to ischemic effects. It is noted that after 14 days in all wall membranes the proliferative activity of fiboblasts was remained. Under the conditions of the correction with the carbacetam after 2 hours, the structural positive dynamics became more pronounced and increased to a maximum level after 7 days of the experiment. The number of the modified and exfoliated endothelial cells decreased, and the condition of smooth myocytes increased. Histologically, the gradual restoration of endothelial coverage of the intima was established. As follows, ischemia and reperfusion cause vascular remodeling after 1 hour with a peak of the manifestations after 1 day of the reperfusion, which includes edematous syndrome, dystrophic-degenerative changes with an inflammatory response to the damage, and in the late reperfusion period increased a fibroblasts activity. Gradual return of morphological changes occurs after 14 days of the experiment. Under the conditions of correction, the acceleration of the remodeling with stabilization of the process and the most possible structural restoration after 7 days of the study was noted.

scholarly journals Effects of abdominal sepsis in the healing of abdominal wall: experimental study in rats

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 38-44 ◽  
Author(s):  
Alexandre Malta Brandão ◽  
Naiara Galvão da Silva ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Breaking Strength ◽  
Abdominal Sepsis ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Postoperative Sepsis ◽  
Silk Thread ◽  
The Third ◽  
The Mean

PURPOSE: To evaluate the influence of sepsis in the process of wound healing in the abdominal wall. METHODS: 40 rats divided into two groups of twenty animals: group of study (E) - septic, and the control group (C) - not septic. The two groups were divided into subgroups of 10 to be killed on the third day (n = 10) or seventh (n = 10) postoperative. Sepsis was induced by ligation and puncture of the cecum. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. On the day of re-laparotomy, the abdominal wall was removed for analysis of the breaking strength and histopathological analysis. RESULTS: The mean breaking strength was at third day: E group (1.44 ± 1.22) and C group (0.35 ± 0.46). At seventh day the mean breaking strength was: E group (13.01 ± 7.09) and C group (11.66 ± 7.38). There was statistical difference in tensile strength. E group at third day with control group (p = 0.019). CONCLUSION: The induction of peritoneal sepsis reduced the breaking strength of the abdominal wall on the third day after surgery.

scholarly journals Effects of bromopride on abdominal wall healing with induced peritoneal sepsis after segmental colectomy and colonic anastomosis in rats

2011 ◽  
Vol 26 (6) ◽  
pp. 433-437 ◽  
Author(s):  
Marcos Vinícius Melo de Oliveira ◽  
Alexandre Malta Brandão ◽  
Pedro Henrique Alves de Morais ◽  
Naiara Galvão da Silva ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Saline Solution ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Left Colon ◽  
Control Group ◽  
The Third ◽  
Segmental Colectomy ◽  
Group B ◽  
Harmful Effects

PURPOSE: Evaluate the effects of bromopride on abdominal wall healing of rats with induced peritoneal sepsis after segmental colectomy and colonic anastomosis. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of bromopride (bromopride group - B) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (GB3 and GC3) or seventh postoperative day (GB7 and GC7). It was analyzed the following characteristics: breaking strength of the abdominal wall's wound; surgical and histopathological features of the abdominal wall; and clinical features of the rats. RESULTS: There was no difference between the groups in relation to the weight of the rats and the breaking strength of the abdominal wall's wound. The GB7 group presented less edema and less quantity of fibrin during histopathological evaluation compared to the GC7 group. CONCLUSION: Bromopride did not have harmful effects on the healing of abdominal wall in rats.

scholarly journals Role of insulin in oleic acid-induced acute lung injury in rat model

2021 ◽  
Vol 65 ◽  
pp. 103-108
Author(s):  
Sudhashekhar Kumar ◽  
Priyanka Bhagat ◽  
Shashikant C. U. Patne ◽  
Ratna Pandey
Keyword(s):  
Acute Lung Injury ◽  
Oleic Acid ◽  
Lung Injury ◽  
Water Content ◽  
Histological Examination ◽  
Rat Model ◽  
Control Group ◽  
Progressive Decrease ◽  
Immunomodulatory Agent ◽  
Group I

Objectives: Acute lung injury (ALI) is an inflammatory condition, therefore, this study was undertaken to determine the effect of insulin (an immunomodulatory agent) in oleic acid (OA)-induced ALI in rat model. Materials and Methods: The experiments were performed on adult male albino rats (total n = 18). The trachea, jugular vein and carotid artery of anaesthetised adult rats were cannulated to keep the respiratory tract patent, deliver saline/drugs and recording of blood pressure, respectively. Animals were divided into three groups. In Group I (control group), normal saline (75 μL) was injected and this group served as control group. In Group II (OA group), OA (75 μL) was administered to induce ALI in rats. In Group III (insulin + OA), OA (75 μL) was injected in insulin pre-treated rats. Respiratory frequency (RF), heart rate (HR) and mean arterial pressure (MAP) were recorded on computerised chart recorder; arterial blood sample was collected to determine PaO2/FiO2. Further, pulmonary water content was determined, and histological examination of the lung was done in all animals. Results: Injection of OA produced ALI indicated by significant increase in RF by 30 min followed by progressive decrease and ultimately death of animal. Significant increase in the pulmonary water content and decrease in PaO2/FiO2 were observed in these animals. Histological examination of lungs showed damage to the lung parenchyma. An immediate decrease in HR and MAP followed by some improvement and then progressive decrease was also observed. Conclusions: Insulin (an immunomodulatory agent) pre-treatment delayed initiation of OA-induced ALI as indicated by protection against OA-induced severe alteration in the RF in the initial stage and less lung injury in histological examination, although; it could not alter the overall course of the disease.

Clear Cell Carcinoma of the abdominal wall

2017 ◽  
Vol 89 (6) ◽  
pp. 40-43 ◽  
Author(s):  
Ewa Kostrzeba ◽  
M. Barczyk ◽  
M. Wichtowski ◽  
R. Garstecki ◽  
D. Murawa
Keyword(s):  
Cesarean Section ◽  
Cell Carcinoma ◽  
Abdominal Wall ◽  
Clear Cell ◽  
Clear Cell Carcinoma ◽  
Good Condition ◽  
Gjb2 Gene ◽  
Control Group ◽  
Exon 2 ◽  
Group I

Background: Clear cell carcinoma in scars after cesarean section is extremely rare, with only 22 cases reported in the literature. Management of this condition needs to be further explored. Here, we report of a patient with clear cell carcinoma of the abdominal wall that developed 35 years after cesarean section. Case Report: The material of the study was a group of 61 patients divided into two groups. Group I – 35 deaf or with profound sensorineural hearing loss children (the pupils of the deaf and hard of hearing school), aged 5–17 years (average 9,2 years), 14 males, 21 females, II – control group comprised 26 normal hearing patients, aged 5–16 years (average 10,4 years), 14 males, 12 females (patients of Department of Pediatric Otolaryngology, Audiology and Phoniatrics, Medical University of Lodz). In both groups, exon 2 sequencing of GJB2 gene was performed. Results. A 58-year-old woman was admitted to our department due to abdominal pain and a progressively growing mass in the abdominal wall. Based on biopsy, a preliminary diagnosis of clear cell carcinoma was made. A wide surgical excision of the tumor with clear margins, hysterectomy with bilateral salpingo-oophorectomy, and abdominal wall reconstruction using synthetic mesh were performed. The patient was discharged in good condition after fifteen days of hospitalization. The patient remained recurrence-free 6 months after the treatment. Conclusions: T Lack of standardized management of rare malignant transformations hinders patient care. Due to a growing number of cesarean deliveries, we can expect clear cell carcinoma prevalence of the abdominal wall to increase. Therefore, patients and clinicians should attend to any pain, itching, or change in the size of abdominal wall scars.

scholarly journals Histological aspects of remodeling of the placental complex in pregnancy complicated by late preeclampsia with different options for its correction

2021 ◽  
Vol 25 (3) ◽  
pp. 394-397
Author(s):  
U. Ya. Franchuk ◽  
S.V. Khmil ◽  
Yu. M. Orel
Keyword(s):  
Metabolic Syndrome ◽  
Histological Examination ◽  
Therapy Group ◽  
Cell Structure ◽  
Pathological Examination ◽  
Control Group ◽  
Study Group ◽  
Group Iii ◽  
Group I ◽  
Calcium Magnesium

Annotation. The study of the problem of preeclampsia is still relevant because it is one of the most common complications of pregnancy and is one of the causes of maternal mortality, ranking second after obstetric bleeding. Perinatal mortality rates vary widely and depend on the severity and duration of late preeclampsia. The aim of the study was to investigate the histological aspects of placental complex remodeling in patients whose pregnancy was complicated by late preeclampsia on the background of metabolic syndrome. Women, pregnancy complicated by late gestosis on the background of metabolic syndrome, were divided into two groups: patients of group I received standard protocol therapy group (31 women), women of group II received preventive, modified, pathogenetically justified therapy – protocol treatment + vitamin D in dose of 2000 IU per day, L-arginine, calcium, magnesium sulfate and salicylates 1 sachet for 10 days (38 patients), group III consisted of 40 women whose physiological pregnancy was without complications and extragenital pathology. The obtained histological specimens were examined using a Nikon Eclipse Ci-E light microscope. The algorithm of histological examination was regulated by the generally accepted protocol of pathological examination of manure. In the first study group there were histological changes, which should be regarded as morphological markers of functional disorders in the system “mother-placenta-foetus”, accompanying late preeclampsia. Metaplasia of the cylindrical epithelium of the foetal surface into a multi-layered flat one was observed in the placental amnion, and in some places increased proliferative activity of epitheliocytes was observed, which formed plaque-like structures and growths. In the second study group, which received a modified treatment, the tissue structure of the manure was also characterized by deviations from the histological picture of the control group. However, the intensity and prevalence of pathological changes were significantly lower than in the first study group. In most of the studied histological sections, the cell structure of the amniotic epithelium was preserved, desquamation was not observed. Circulatory disorders in the villous chorion were present, but the structure of most vessels remained unchanged. Based on the results of histological examination, it can be concluded that a comprehensive, modified therapy of late preeclampsia on the background of metabolic syndrome, allows you to normalize the structure of the placenta.

scholarly journals Influence of metoclopramide on abdominal wall healing in rats subjected to colonic anastomosis in the presence of peritoneal sepsis induced

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 92-99 ◽  
Author(s):  
Naiara Galvão da Silva ◽  
Alexandre Malta Brandão ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Foreign Body Reaction ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Cecal Ligation ◽  
Left Colon ◽  
Control Group ◽  
Silk Thread ◽  
Inflammatory Infiltration ◽  
Histopathological Evaluation

PURPOSE: To evaluate the effects of metoclopramide on abdominal wall healing in rats in the presence of sepsis. METHODS: 40 rats divided into two groups of twenty animals, subdivided into two subgroups of 10 animals each: group (E) - treated with metoclopramide, and saline-treated control group. The two groups were divided into subgroups of 10 to be killed on the 3rd day (n = 10) or day 7 (n = 10) after surgery. Sepsis was induced by cecal ligation and puncture. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. We measured the breaking strength of the abdominal wall and made the histopathological evaluation. RESULTS: on 3rd day postoperative, the average breaking strength in the E group was 0.83 ± 0.66 and in group C was 0.35 ± 0.46 (p = 0.010). On the seventh day, the breaking strength in group E was11.44 ± 5.07, in group C 11.66 ± 7.38 (p = 1.000). The E7 group showed lower inflammatory infiltration, foreign body reaction, fibrin than control. CONCLUSION: animals treated with metoclopramide had a higher resistance of the abdominal wall on the 3rd postoperative day.

Perioperative neorectal ischemia. Experimental model

2020 ◽  
Vol 15 (4) ◽  
pp. 47-53
Author(s):  
D.D. Morozov ◽  
◽  
O.L. Morozova ◽  
L.O. Severgina ◽  
T.D. Marchuk ◽  
...  
Keyword(s):  
Histological Examination ◽  
Postoperative Period ◽  
Long Term Results ◽  
Intestinal Tissue ◽  
Laboratory Rats ◽  
Group I ◽  
Factor C ◽  
Tissue Tension ◽  
Hypoxia Markers

Objective. To experimentally determine and analyze the role of perioperative tissue tension in the intestinal area brought down to the proper topographic and anatomical position (neorectum) and its vascularization impairments during anorectoplasty. Materials and methods. This experiment performed on laboratory rats consisted of 3 parts: clinical (creation of an operation model and assessment of clinical parameters in the early postoperative period); morphological (histological examination of biopsy specimens taken from the surgical area); and laboratory (measurement of tissue hypoxia markers (including vascular endothelial growth factor-C (VEGF-C) and hypoxia-induced factor 1-alpha (HIF-1-alpha)) in serum and intestinal homogenate using enzyme-linked immunosorbent assay (ELISA) (specimens were collected 3 days postoperatively). Forty laboratory rats weighing from 370 to 550 g (mean weight 482 g) were divided into four equal groups: group I – ‘impaired neorectal vascularization;’ group II – ‘neorectal tension;’ group III – ‘rectal replantation;’ and the control group. During the first stage of the experiment (3 days), we assessed overall condition of the animals, number of stools, type of defecation, and pain severity. The animals received no treatment in the postoperative period. On day 3, all rats were euthanized. Tissue specimens were collected from the neorectum to evaluate its morphological characteristics and to produce intestinal homogenate for subsequent ELISA. Blood specimens were obtained by puncturing the left ventricle and then centrifuged at 3000g for 15 minutes. Blood supernatants and neorectum tissues were stored at -80°C for maximum 1 month before testing. Intestinal tissue samples for histological examination were stored in a 10% formalin solution. The levels of hypoxia biomarkers were measured in serum and intestinal homogenate using the Stat Fax 2100. Data analysis was conducted using the SPSS software (with no preliminary calculation of the sample size). We used the Mann–Whitney U-test [H.B.Mann, D.R.Whitney] and Kruskal–Wallis test [W.Kruskall, W.A.Walles]. The differences were considered significant at р < 0.05. Results. The most common morphological changes were caused by impaired vascularization of the rectum. We have found a significant increase (p < 0.001) in the levels of hypoxia markers in intestinal homogenates in all three experimental compared to controls. However, there was no significant difference in the levels of these markers between the experimental groups. The defecation process was abnormal in rats from groups I and II. Conclusion. In anorectoplasty, excessive mobilization of the rectum with its improper vascularization and formation of the anus under conditions of intestinal tissue tension lead to perioperative neorectal ischemia. This experiment should be continued to evaluate long-term results. The proposed models of perioperative neorectal ischemia can be used to analyze the role of many factors that can potentially affect the outcome of anorectal malformation correction. In the future, this problem may be investigated in clinical studies. Key words: anorectal malformations, perioperative ischemia, neorectum, tension, devascularization

scholarly journals Acellular dermal graft for repair of abdominal wall defects in rabbits

2006 ◽  
Vol 77 (2) ◽  
Author(s):  
A.K. Gangwar ◽  
A.K. Sharma ◽  
Naveen Kumar ◽  
N. Kumar ◽  
S.K. Maiti ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Test Group ◽  
Control Group ◽  
Abdominal Wall Defects ◽  
Full Thickness ◽  
Dermal Graft ◽  
Group I ◽  
Group A ◽  
Acellular Dermal

Sixteen clinically healthy New Zealand white rabbits of either sex were divided into 2 equal groups (I and II) of 8 animals each. Under thiopental sodium (2.5 %) anaesthesia a 2 × 3 cm full-thickness abdominal wall defect in the mid-ventral abdominal wall was created and repaired with an acellular dermal graft (ADG) in all the animals of group I (test group). In animals of group II (control group) a full-thickness linear midline abdominal muscular wall incision was made and repaired with a continuous suture pattern using 2-0 nylon.

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