scholarly journals Effects of abdominal sepsis in the healing of abdominal wall: experimental study in rats

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 38-44 ◽  
Author(s):  
Alexandre Malta Brandão ◽  
Naiara Galvão da Silva ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Breaking Strength ◽  
Abdominal Sepsis ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Postoperative Sepsis ◽  
Silk Thread ◽  
The Third ◽  
The Mean

PURPOSE: To evaluate the influence of sepsis in the process of wound healing in the abdominal wall. METHODS: 40 rats divided into two groups of twenty animals: group of study (E) - septic, and the control group (C) - not septic. The two groups were divided into subgroups of 10 to be killed on the third day (n = 10) or seventh (n = 10) postoperative. Sepsis was induced by ligation and puncture of the cecum. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. On the day of re-laparotomy, the abdominal wall was removed for analysis of the breaking strength and histopathological analysis. RESULTS: The mean breaking strength was at third day: E group (1.44 ± 1.22) and C group (0.35 ± 0.46). At seventh day the mean breaking strength was: E group (13.01 ± 7.09) and C group (11.66 ± 7.38). There was statistical difference in tensile strength. E group at third day with control group (p = 0.019). CONCLUSION: The induction of peritoneal sepsis reduced the breaking strength of the abdominal wall on the third day after surgery.

scholarly journals Effects of bromopride on abdominal wall healing with induced peritoneal sepsis after segmental colectomy and colonic anastomosis in rats

2011 ◽  
Vol 26 (6) ◽  
pp. 433-437 ◽  
Author(s):  
Marcos Vinícius Melo de Oliveira ◽  
Alexandre Malta Brandão ◽  
Pedro Henrique Alves de Morais ◽  
Naiara Galvão da Silva ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Saline Solution ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Left Colon ◽  
Control Group ◽  
The Third ◽  
Segmental Colectomy ◽  
Group B ◽  
Harmful Effects

PURPOSE: Evaluate the effects of bromopride on abdominal wall healing of rats with induced peritoneal sepsis after segmental colectomy and colonic anastomosis. METHODS: Forty rats underwent sectioning of the left colon and end-to-end anastomosis and were divided into two groups of 20 animals for the administration of bromopride (bromopride group - B) or saline solution (control group - C). Each group was divided into subgroups of 10 animals each to be killed on the third (GB3 and GC3) or seventh postoperative day (GB7 and GC7). It was analyzed the following characteristics: breaking strength of the abdominal wall's wound; surgical and histopathological features of the abdominal wall; and clinical features of the rats. RESULTS: There was no difference between the groups in relation to the weight of the rats and the breaking strength of the abdominal wall's wound. The GB7 group presented less edema and less quantity of fibrin during histopathological evaluation compared to the GC7 group. CONCLUSION: Bromopride did not have harmful effects on the healing of abdominal wall in rats.

scholarly journals Influence of metoclopramide on abdominal wall healing in rats subjected to colonic anastomosis in the presence of peritoneal sepsis induced

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 92-99 ◽  
Author(s):  
Naiara Galvão da Silva ◽  
Alexandre Malta Brandão ◽  
Marcos Vinícius Melo de Oliveira ◽  
Pedro Henrique Alves de Morais ◽  
Silvana Marques e Silva ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Foreign Body Reaction ◽  
Colonic Anastomosis ◽  
Breaking Strength ◽  
Cecal Ligation ◽  
Left Colon ◽  
Control Group ◽  
Silk Thread ◽  
Inflammatory Infiltration ◽  
Histopathological Evaluation

PURPOSE: To evaluate the effects of metoclopramide on abdominal wall healing in rats in the presence of sepsis. METHODS: 40 rats divided into two groups of twenty animals, subdivided into two subgroups of 10 animals each: group (E) - treated with metoclopramide, and saline-treated control group. The two groups were divided into subgroups of 10 to be killed on the 3rd day (n = 10) or day 7 (n = 10) after surgery. Sepsis was induced by cecal ligation and puncture. We performed also the section and anastomosis in left colon. The synthesis of the abdominal wall was made with 3-0 silk thread. We measured the breaking strength of the abdominal wall and made the histopathological evaluation. RESULTS: on 3rd day postoperative, the average breaking strength in the E group was 0.83 ± 0.66 and in group C was 0.35 ± 0.46 (p = 0.010). On the seventh day, the breaking strength in group E was11.44 ± 5.07, in group C 11.66 ± 7.38 (p = 1.000). The E7 group showed lower inflammatory infiltration, foreign body reaction, fibrin than control. CONCLUSION: animals treated with metoclopramide had a higher resistance of the abdominal wall on the 3rd postoperative day.

scholarly journals Evaluation of the effects of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats undergoing segmental resection and anastomosis of the left colon

2012 ◽  
Vol 27 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Pedro Henrique Alves de Morais ◽  
Igor Eduardo Caetano de Farias ◽  
Leonardo de Castro Durães ◽  
Fabiana Pirani Carneiro ◽  
Paulo Gonçalves de Oliveira ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Wound Healing ◽  
Tensile Strength ◽  
Abdominal Wall ◽  
Left Colon ◽  
Control Group ◽  
Histopathological Analysis ◽  
Abdominal Closure ◽  
Carbon Dioxide Pneumoperitoneum ◽  
Resection And Anastomosis

PURPOSE: To evaluate the influence of carbon dioxide pneumoperitoneum on abdominal wall wound healing in rats. METHODS: Eighty rats underwent laparotomy, segmental left colon resection, and anastomosis. The animals were divided into three experimental groups and one control group: EI = pneumoperitoneum for 30 minutes before laparotomy (n=20); EII = pneumoperitoneum for 30 minutes after abdominal closure (n=20); EIII = pneumoperitoneum for 30 minutes before laparotomy and 30 minutes after abdominal closure (n=20); C = control group, without pneumoperitoneum (n=20). In each group, 10 animals were killed 7 days and 10 animals 14 days postoperatively. A segment of the abdominal wall was resected and subjected to tensile strength testing. Another segment of abdominal muscle was used for histopathological analysis; the specimens were fixed in formalin and stained with hematoxylin and eosin. RESULTS: There were no differences in histopathology and tensile strength values among animals in the experimental and control groups 7 or 14 days after surgery. CONCLUSION: Under the present experimental conditions, carbon dioxide pneumoperitoneum did not interfere with abdominal wall wound healing.

scholarly journals Effects of topical gel formulation of Ficus carica latex on cutaneous leishmaniasis induced by Leishmania major in BALB/c mice

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ali Pouryousef ◽  
Erfan Eslami ◽  
Sepehr Shahriarirad ◽  
Sina Zoghi ◽  
Mehdi Emami ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Murine Model ◽  
Leishmania Major ◽  
Considerable Effect ◽  
Ficus Carica ◽  
Control Group ◽  
Topical Gel ◽  
The Third ◽  
Mean Size ◽  
The Mean

Abstract Objectives The current study aimed to evaluate the effects of Ficus carica latex on the treatment of cutaneous leishmaniasis (CL), induced by Leishmania major. A 5% topical gel with F. carica latex was prepared. BALB/c mice were infected by inoculation of amastigotes form of L. major. Thirty BALB/c mice were divided into five groups, where the first group was treated daily, the second group twice per day, and the third group every other day with the 5% topical gel, for 3 weeks. The sizes of the lesions were measured before and during the course of treatment. Results Although the mean size of lesions in the mice group treated with the 5% F. carica gel, especially in the group receiving daily treatment, was less than the mean size of the lesions in the control group, yet, the differences was not statistically significant (p > 0.05). The findings of the current study demonstrated that the 5% F. carica latex with a 3-week course of treatment had no considerable effect in recovery or control of CL induced by L. major in the murine model. Using higher concentration of F. carica latex and with longer treatment lengths may increase its efficacy in the treatment of CL.

scholarly journals DOES THE ASSOCIATION OF TACROLIMUS AND MYCOPHENOLATE MOFETIL CHANGE THE HEALING OF THE ABDOMINAL WALL? STUDY IN RATS SUBMITTED TO ISCHEMIA AND KIDNEY REPERFUSION

2020 ◽  
Vol 33 (4) ◽  
Author(s):  
André Luís Conde WATANABE ◽  
Jorge Eduardo Fouto MATIAS
Keyword(s):  
Mycophenolate Mofetil ◽  
Abdominal Wall ◽  
Histological Examination ◽  
Postoperative Period ◽  
Breaking Strength ◽  
Immunosuppressive Agents ◽  
Control Group ◽  
Strength Measurement ◽  
Standard Operation ◽  
Group I

ABSTRACT Background: Tacrolimus and mycophenolate mofetil are immunosuppressive agents widely used on the postoperative period of the transplants. Aim: To evaluate the influence of the association of them on the abdominal wall healing in rats. Methods: Thirty-six Wistar rats were randomly assigned in three groups of 12. On the early postoperative period, four of the control group and three of the experimental groups died. The three groups were nominated as follow: control group (GC, n=8); group I (GI, n=11, standard operation, mycophenolate mofetil and tacrolimus); group II (GII, n=10, standard operation, mycophenolate mofetil and tacrolimus). The standard operation consisted of right total nephrectomy and 20 min ischemia of the left kidney followed by reperfusion. Both NaCl 0.9% and the immunosuppressive agents were administered starting on the first postoperative day and continuing daily until the day of death on the 14th day. On the day of their deaths, two strips of the anterior abdominal wall were collected and submitted to breaking strength measurement and histological examination. Results: There were no significant differences in wound infection rates (p=0,175), in the breaking strength measurement and in the histological examination among the three groups. Conclusion: The combination of the immunosuppressive agents used in the study associated with renal ischemia and reperfusion does not interfere in the abdominal wall healing of rats.

Circulating immunoreactive somatostatin in man. Effect of age, pregnancy, growth hormone deficiency and achlorhydria

1985 ◽  
Vol 110 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Susan M. Webb ◽  
John A. H. Wass ◽  
Erica Penman ◽  
M. Murphy ◽  
José Serrano ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Hormone Deficiency ◽  
Pernicious Anaemia ◽  
Hormone Deficiency ◽  
Control Group ◽  
Normal Children ◽  
The Third ◽  
Group 12 ◽  
The Mean ◽  
Effect Of Age

Abstract. Plasma immunoreactive somatostatin (IRS) levels were measured fasting at 09.00 h in groups of adult individuals and children of different ages, as well as in pregnant women, in patients with pernicious anaemia documented to be achlorhydric, and in children with growth hormone deficiency. There was a gradual rise in the mean level of IRS from the third decade (mean 35.8 ± 3.8 pg/ml), which reached significance at the seventh (61.1 ± 8.4 pg/ml), eighth (66.7 ± 5 pg/ml) and ninth decade (82.6 ± 13.8 pg/ml). No change was observed in the second 28.3 ± 3.8 pg/ml) and third (31.1 ± 3.2 pg/ml) trimester of pregnancy when compared with matched, non-pregnant controls (29.7 ± 2.2 pg/ml); however, the children aged under 2 years (69.6 ± 11.2 pg/ml) had significantly higher values than the eldest group (12 to 16 years old) (46.3 ± 7.2 pg/ml) (P < 0.05). In achlorhydric patients, basal (27.2 ± 3.7 pg/ml; P < 0.01) and postprandial IRS (42.8 ± 7.7 pg/ml; P < 0.001) was significantly lower than in a matched, normal control group (basal 59.4 ± 7.2; postprandial 132.1 ± 26.3 pg/ml). Growth hormone deficiency was not associated with any differences in circulating IRS, basally or after insulin hypoglycaemia, when compared with values in normal children. These results would suggest, 1) that age has a significant effect on plasma IRS, and should be considered in the interpretation of fasting plasma levels of IRS; 2) that pregnancy and growth hormone deficiency is not accompanied by any changes in circulating IRS and presumably, somatostatin binding proteins; 3) that gastric acid is necessary for a normal release of IRS from the gastrointestinal tract to the circulation.

scholarly journals The Effect of Subconjunctival Bevacizumab on Angiogenesis in Rabbit Model

2020 ◽  
Vol 55 (4) ◽  
pp. 290
Author(s):  
Nurwasis Nurwasis ◽  
Diana Yuliawati ◽  
Evelyn Komaratih ◽  
Heriyawati Heriyawati
Keyword(s):  
Blood Vessels ◽  
Healing Process ◽  
Rabbit Model ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Histopathological Analysis ◽  
Subconjunctival Injection ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

The wound healing process is major cause of glaucoma surgery failure and enhances the incapability of controlling the IOP. This aim of study was to evaluate the angiogenesis effect of Bevacizumab injection on the amount and density of blood vessels in the rabbit model after trabeculectomy. It was a true experimental study using 16 eyes of 16 New Zealand White Rabbit (Oryctolagus cuniculus) that divided into two groups. The control group was treated with 0.05 ml of Balanced Saline Solution (BSS). The experimental group was treated with subconjunctival injection of 1.25 mg Bevacizumab in 0.05 BSS. All rabbit was sacrificed, and the eye was enucleated. Thus, the bleb area was dissected after 14 days. Histopathological analysis was performed to evaluate the amount and density of blood vessels. The mean amount of blood vessels in the control group was 22.63 ± 11.02, and the experimental group was 14,75 ± 4.92 (p=0.043). The mean of blood vessel density in the control group was 19.10 ± 1.69 %, and the experimental group was 16.53 ± 2.90 % (p=0.029)%. There was a statistically significant difference between the two groups (p<0.05). Subconjunctival bevacizumab in the rabbit model reduces the amount and density of blood vessels compared with subconjunctival BSS injection.

scholarly journals Different Doses of Intraumbilical Oxytocin on the Third Stage of Labor

2018 ◽  
pp. 155
Author(s):  
Nurul Islamy ◽  
Nuswil Bernolian ◽  
Firmansyah BasiR ◽  
Theodorus Theodorus
Keyword(s):  
Intervention Group ◽  
Control Group ◽  
Chi Square ◽  
Randomised Study ◽  
The Third ◽  
Third Stage Of Labor ◽  
Treatment Dose ◽  
Third Stage ◽  
The Mean ◽  
Post Hoc

  Objective: To compare the dose of oxytocin injected intraumbilicalltowards the duration of the third stage, blood loss volume, hemoglobinand hematocrit.   Methods: This study is a prospective randomised study with acontrol. The control group was given an intramuscular injection of10 IU oxytocin. The intervention of the three groups intraumbilicaloxytocin treatment dose of 10 IU, 20 IU and 30 IU diluted in 50 ml ofnormal saline solution and administered intraumbilically. Thesample selection by purposive sampling and the distribution groupbased on systematic random sampling (10 samples each). Data weretaken from the period April 2016-January 2017 with the inclusionand exclusion criteria. Data were analysed using Chi-square, T-test,ANOVA and Post hoc tests.   Results: Characteristics study for variables of age, occupation,parity, education, episiotomy and neonates weight showed homogeneouscharacteristics. The mean duration of the third stage forall groups was between 366.7  159.0 seconds and 440.1  244.99seconds. While the average number of postpartum haemorrhage forall group 61.894  226.3ml and 309.5  110.26 ml. There were nodifferences in the dose of oxytocin on the duration of the thirdstage (p> 0.05) and the amount of bleeding (p> 0.005). There was adifference of haemoglobin between intervention group of oxytocindose of 10 IU and 30 IU intraumbilical (p = 0.031). There was nodifference between the mean hematocrit levels between the groups(p> 0.005).   Conclusion: There were no differences in the dose of oxytocinintraumbilical towards the duration of the third stage, the amountof bleeding and hematocrit levels. The decrease of haemoglobingreater in 30 IU intraumbilical significantly. Keywords: duration of the third stage, haemoglobin and hematocrit,oxytocin intraumbilical, the amount of bleeding

scholarly journals Comparison of Salivary Alpha-Amylase, Sialic Acid, and pH in Pregnant and Nonpregnant Subjects

2021 ◽  
Author(s):  
Masoomeh Shirzaiy ◽  
Zohreh Dalirsani
Keyword(s):  
Sialic Acid ◽  
Pregnant Women ◽  
First Trimester ◽  
Control Group ◽  
Case Group ◽  
Third Trimester ◽  
The Third ◽  
Significant Difference ◽  
Salivary Ph ◽  
The Mean

Abstract Objectives During pregnancy, systemic physiological alterations lead to some changes in the oral cavity, which could prepare the mouth environment for oral and dental problems. This study was aimed to investigate salivary α-amylase, sialic acid levels, and pH levels in pregnant and nonpregnant females. Materials and Methods In this analytical, case–control study, unstimulated saliva samples were collected with spiting method from 35 pregnant women (case group) and 35 nonpregnant women (control group) and transferred to the laboratory to assess salivary α-amylase, sialic acid, and pH levels. Data were analyzed by SPSS (version: 19) software through statistical methods of independent t-test and analysis of variance. Results The mean sialic acid levels were 2.285 ± 1.230 mg/dL in pregnant and 2.744 ± 1.326 in nonpregnant women without any significant difference (p = 0.138). The mean salivary α-amylase concentrations were 2.461 ± 1.869 U/L and 2.439 ± 2.058 U/L, respectively, in pregnant and nonpregnant women, with no significant difference (p = 0.963).The mean salivary pH in nonpregnant women was significantly more than that in pregnant women (7.845 ± 0.430 and 6.868 ± 0.413, respectively) (p < 0.001). Also, the mean salivary pH levels in pregnant women were 7.474 ± 0.420 in the first trimester, 6.868 ± 0.413 in the second trimester, and 6.568 ± 0.387 in the third trimester, which were significantly different (p < 0.001). Conclusion Salivary sialic acid and α-amylase levels among pregnant women were no different from those of other subjects. During pregnancy, the salivary pH significantly reduced, and the mean salivary pH during pregnancy had a decreasing trend from the first trimester to the third trimester.

scholarly journals TEAMMATES CONSULT STRATEGY TOWARDS STUDENTS’ MOTIVATION AND READING NARRATIVE TEXT

2018 ◽  
Vol 5 (1) ◽  
pp. 7
Author(s):  
Fathurrahman Imran ◽  
K. Dedy Sandiarsa S.
Keyword(s):  
Data Analysis ◽  
Experimental Research ◽  
Research Data ◽  
Narrative Text ◽  
Control Group ◽  
The Third ◽  
The Mean ◽  
And Control ◽  
Experimental Group

This research was generally attempted to find the effect of Teammates Consult Strategy towards students’ motivation and reading narrative text at FPBS IKIP Mataram. This research was an experimental research with MANOVA as the data analysis. The population of this research was the students of the third semester of FPBS students. The sample of the research was 45 students consisted of two classes. From the research data found that with the mean score of motivation was 81,04 and control group was 78,00. With the significance 0.000 < 0,05. While the mean for the reading of experimental group was 82,54 and control group was 79,09 with the significance 0.000 < 0,05. It can be concluded that the result of the research showed that there was a significant effect of Teammates Consult strategy towards students’ motivation, there was a significant effect of Teammates consult strategy toward reading narrative text, there was a significant effect of motivation and reading narrative text, there was a significant effect of Teammates Consult strategy towards students motivation and reading narrative text and there was no correlation between motivation and reading narrative text.

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